Open Access

ARTICLE

Organogenesis and plant regeneration of Arachis villosa Benth. (Leguminosae) through leaf culture

MARÍA LAURA FONTANA, LUIS AMADO MROGINSKI, HEBE YOLANDA REY*

Instituto de Botánica del Nordeste (IBONE). Facultad de Ciencias Agrarias. Universidad Nacional del Nordeste, Corrientes, Argentina.
* Address correspondence to: Hebe Yolanda Rey. E-mail: heberey@agr.unne.edu.ar

BIOCELL 2009, 33(3), 179-186. https://doi.org/10.32604/biocell.2009.33.179

Abstract

With the aim of developing an efficient plant regeneration protocol, leaflet explants of three accessions of Arachis villosa Benth. (S2866, S2867 and L97) were cultured on basic Murashige and Skoog medium supplemented with different combinations of plant growth regulators: α-naphthalenacetic acid, indole-3-butyric acid, 6-benzylaminopurine, kinetin and thidiazuron. The accession L97 was the only one able to differentiate buds through indirect organogenesis. The most suitable combination for bud regeneration was the basic medium added with 13.62 μM thidiazuron and 4.44 μM 6-benzylaminopurine. These results show the important role of the genotype in morphogenetic responses and the organogenetic effect of thidiazuron in Arachis villosa accession L97. A thidiazuron lacking media (only 0.54 μM α-naphthalenacetic acid, 13.95 μM kinetin and 13.32 μM 6-benzylaminopurine were added) promoted the elongation of the regenerated buds. Adventitious rooting was achieved 90 days after the isolated shoots were transferred to a rooting medium containing 0.54 μM α-naphthalenacetic acid.

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