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Brief Note: A glass bead protocol for recovery of host cell free Ehrlichia canis and quantification by Sybr-green real-time PCR

G. P. CARDOZO1 , E. V. SANTOS1 , A. L. FACHIN1, S. C. FRANÇA1 , AND M. MARINS1,2,*

1. Unidade de Biotecnologia, UNAERP, Ribeirão Preto, Brazil.
2. Heranza – Biotecnologia, Ribeirão Preto, Brazil.

*Address correspondence to: Mozart Marins. Unidade de Biotecnologia, UNAERP, Ribeirão Preto, Brazil. Tel.: (+55-16) 3603 6892. Fax: (+55 16) 3603 7030. E-mail: email

BIOCELL 2011, 35(1), 35-36. https://doi.org/10.32604/biocell.2011.35.035

Abstract

E. canis infection of the canine cell line DH82 is a routine in studies with this bacteria. A protocol for isolation of host cell free bacteria was developed based on the use of glass beads. Improvement of infection with E. canis isolated by this method was detected by real-time PCR.

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APA Style
, G.P.C., , E.V.S., FACHIN, A.L., , S.C.F., MARINS, A.M. (2011). Brief note: A glass bead protocol for recovery of host cell free <i>ehrlichia canis</i> and quantification by sybr-green real-time PCR. BIOCELL, 35(1), 35-36. https://doi.org/10.32604/biocell.2011.35.035
Vancouver Style
GPC, EVS, FACHIN AL, SCF, MARINS AM. Brief note: A glass bead protocol for recovery of host cell free <i>ehrlichia canis</i> and quantification by sybr-green real-time PCR. BIOCELL . 2011;35(1):35-36 https://doi.org/10.32604/biocell.2011.35.035
IEEE Style
G.P.C. , E.V.S. , A.L. FACHIN, S.C.F. , and A.M. MARINS "Brief Note: A glass bead protocol for recovery of host cell free <i>Ehrlichia canis</i> and quantification by Sybr-green real-time PCR," BIOCELL , vol. 35, no. 1, pp. 35-36. 2011. https://doi.org/10.32604/biocell.2011.35.035

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