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Brief Note: A glass bead protocol for recovery of host cell free Ehrlichia canis and quantification by Sybr-green real-time PCR

G. P. CARDOZO1 , E. V. SANTOS1 , A. L. FACHIN1, S. C. FRANÇA1 , AND M. MARINS1,2,*

1. Unidade de Biotecnologia, UNAERP, Ribeirão Preto, Brazil.
2. Heranza – Biotecnologia, Ribeirão Preto, Brazil.

*Address correspondence to: Mozart Marins. Unidade de Biotecnologia, UNAERP, Ribeirão Preto, Brazil. Tel.: (+55-16) 3603 6892. Fax: (+55 16) 3603 7030. E-mail:

BIOCELL 2011, 35(1), 35-36. https://doi.org/10.32604/biocell.2011.35.035

Abstract

E. canis infection of the canine cell line DH82 is a routine in studies with this bacteria. A protocol for isolation of host cell free bacteria was developed based on the use of glass beads. Improvement of infection with E. canis isolated by this method was detected by real-time PCR.

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CARDOZO, G. P., FRANÇA, S. C. (2011). Brief Note: A glass bead protocol for recovery of host cell free Ehrlichia canis and quantification by Sybr-green real-time PCR. BIOCELL, 35(1), 35–36.

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This work is licensed under a Creative Commons Attribution 4.0 International License , which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
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