Open Access

ARTICLE

Angiotensin II stimulates expression of transcription factors c-Jun and c-Fos in cyclosporine induced human gingival fibroblasts

Tamilselvan SUBRAMANI^1,2, Suresh RAO², Kamatchiammal SENTHILKUMAR³, Soundararajan PERIASAMY⁴, Noorjahan Banu ALITHEEN¹

1Department of Cell and Molecular Biology, Faculty of Biotechnology and Biomolecular Sciences, Universiti Putra Malaysia, Serdang, Malaysia.
2 Department of Periodontics, Faculty of Dental Science, Sri Ramachandra University, Chennai, India.
3 Virology Division, National Environmental Engineering Research Institute, Chennai, India.
4 Department of Nephrology, Faculty of Medicine, Sri Ramachandra University, Chennai, India.

*Corresponding author:

BIOCELL 2013, 37(3), 71-76. https://doi.org/10.32604/biocell.2013.37.071

Abstract

The present study demonstrates that the expression of c-Jun and c-Fos are elevated in gingival fibroblast cells treated with angiotensin II and cyclosporine. The healthy human gingival tissues were collected and gingival fibroblasts were isolated and cultured. We used RT-PCR and Western blot analysis to identify the expression of c-Jun and c-Fos in cyclosporine and angiotensin II treated human gingival fibroblast cells. We found that angiotensin II in combination with cyclosporine induces c-Jun and c-Fos expressions significantly; however, the angiotensin II antagonist losartan inhibits the expression of c-Jun and c-Fos (p < 0.01). The data suggest that angiotensin II in combination with cyclosporine modulates the expression of c-Jun and c-Fos in human gingival fibroblast cells.

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