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Potential detoxification of aflatoxin B2 using Kluyveromyces lactis and Saccharomyces cerevisiae integrated nanofibers

MAHMOUD MOUSTAFA1,2*, TAREK TAHA3, MOHAMED ELNOUBY4, NEHAL EL-DEEB5, GAMAL HAMAD6, M.A. ABUSAIED7, SULAIMAN ALRUMMAN1
1 Department of Biology, College of Science, King Khalid University, 9004, Abha, Kingdom of Saudi Arabia (KSA)
2 Botany Department, Faculty of Science, South Valley University, Qena, Egypt
3 Environmental Biotechnology Department, Genetic Engineering and Biotechnology Research Institute (GEBRI), City of Scientific Research and Technological Applications (SRTA-City), New Borg El-Arab City, 21934, Alexandria, Egypt
4 Composite and Nanostructured Materials Research Department, Advanced Technology and New Materials Research Institute, City of Scientific Research and Technological Applications (SRTA-City), New Borg El-Arab City, 21934, Alexandria, Egypt.
5 Department of Biopharmaceutical Product Research, Genetic Engineering and Biotechnology Research Institute (GEBRI), City of Scientific Research and Technological Applications (SRTA-City), New Borg El-Arab City, 21934, Alexandria, Egypt.
6 Food Technology Department, Arid Land Cultivation Research Institute (ALCRI), City of Scientific Research and Technological Applications (SRTA-City), New Borg El-Arab City, 21934, Alexandria, Egypt.
7 Polymer Materials Research Department, Advanced Technology and New Materials Research Institute, City of Scientific Research and Technological Applications (SRTA-City), New Borg El-Arab City, 21934, Alexandria, Egypt.
* Address correspondence to: Mahmoud Moustafa

BIOCELL 2017, 41(2-3), 67-73. https://doi.org/10.32604/biocell.2017.41.067

Abstract

Current investigation has shown that human exposure to aflatoxins is not limited to the administration of contaminated cereals, but water is another possible source. This study was aimed to design easily applicable method to eliminate aflatoxin B2 (AFB2) from contaminated drinking water. Electrospinning has been used for preparation of probiotic-coated polyvinyl alcohol (PVA) and cellulose acetate (CA) nanofibers. Both of these hybrid nanofibers were studied by scanning electron microscopy (SEM) and Fourier-transformed infrared spectroscopy (FT-IR). SEM showed the proper coating of probiotic strains (Kluyveromyces lactis CBS 2359 and Saccharomyces cerevisiae ATCC 9763) on both nanofiber types. Different areas (1-5 cm2) of the probiotic-nanofiber hybrid were used to enhance the removal of 20 ng/ml of aflatoxin B2 (AFB2) from prepared AFB2-contaminated water over time. Results revealed that a 5 cm2 area of probiotic-coated PVA nanofibers can eliminate 97.5% of AFB2 as compared to 87.5%, 90.5%, 93.5%, and 95.5%, for 1 cm2, 2 cm2, 3 cm2, and 4 cm2, respectively, while probiotic-coated CA nanofibers were slightly less effective. Nevertheless, the cytotoxicity of probiotics-CA treated water on cultured human fibroblasts was almost 10 times lower than the cytotoxicity recorded in probiotics-PVA treated water. Therefore, results of the current research suggest that probiotics-polymer nanofiber membranes can be used as an extra stage in the water purification system for the treatment of AFB2-contaminated water.

Keywords

Nanofibers, Aflatoxin B2, Cytotoxicity, Water treatment

Cite This Article

MOUSTAFA, M., TAHA, T., ELNOUBY, M., EL-DEEB, N., HAMAD, G. et al. (2017). Potential detoxification of aflatoxin B2 using Kluyveromyces lactis and Saccharomyces cerevisiae integrated nanofibers. BIOCELL, 41(2-3), 67–73.

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This work is licensed under a Creative Commons Attribution 4.0 International License , which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
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