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Spontaneous running wheel improves neuroprotection efficacy of ischemic postconditioning in mice following ischemia/reperfusion injury

Hong YE1,6,#, WeiWei Wang2,#, Yu Ding3,#, XiaoLei Liu4,#, WenJI Jia1, WeiLi Luo1, HuiJuan Fan1, HongQun Zhou1, Jin Wang1, JianLong Ju1, DongMing Zhou7, TianHao Bao5,1,*, YuHong Zhu1,*

1 Department of Neurology, the Second Affiliated Hospital of Kunming Medical University, Kunming, China
2 Department of Cardiology, the Second Affiliated Hospital of Kunming Medical University, Kunming, China
3 Department of Ultrasound, the Second Affiliated Hospital of Kunming Medical University, Kunming, China
4 Department of Neurology, the First Affiliated Hospital of Kunming Medical University, Kunming, China
5 The Mental Health Center of Kunming Medical University, Kunming, China
6 Kunming Medical University, Kunming, China
7 Biomedical Engineering, University of Alberta, Edmonton, Alberta, Canada
# Hong Ye, Yu Ding, WeiWei Wang, XiaoLei Liu contributed equally to this article.

* Address correspondence to: TianHao Bao, email; YuHong Zhu, email

BIOCELL 2018, 42(3), 79-86. https://doi.org/10.32604/biocell.2018.04615

Abstract

Ischemic postconditioning (IP) has been shown to provide protection for ischemia/reperfusion (IR) injury, but its efficacy is limited. In this study we hypothesized that spontaneous running wheel (RW) could improve neuroprotection efficacy of IP for IR. We established mouse models of IR and showed that compared to Sham group, IR group had obvious brain infract and neurological dysfunction. In IR+IP group, brain infract and neurological dysfunction improved compared to IR group. However, in IR+IP+RW group brain infract and neurological dysfunction improved much better. TUNEL assay showed that IP but not RW significantly reduced the number of apoptotic cells after IR. However, the number of apoptotic cells was significantly reduced in RW+IP group. In addition, the levels of pro-apoptotic factors increased in IR group but significantly reduced in IR+IP+RW group, while the levels of antiapoptotic factors decreased in IR group but significantly increased in IR+IP+RW group. Moreover, in IR+IP+RW group, MDA level was further decreased and SOD level was further increased compared to IR+IP group. Finally, both PI3K inhibitor and STAT3 inhibitor significantly worsened brain infract and neurological dysfunction and promoted apoptosis in IR mice. In conclusion, RW combined with IP reduces brain infract and neurological dysfunction in mice after IR, and this is associated with enhanced anti-apoptotic and anti-oxidant benefits via the activation of PI3K and STAT3 pathways.

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APA Style
YE, H., Wang, W., Ding, Y., Liu, X., Jia, W. et al. (2018). Spontaneous running wheel improves neuroprotection efficacy of ischemic postconditioning in mice following ischemia/reperfusion injury. BIOCELL, 42(3), 79-86. https://doi.org/10.32604/biocell.2018.04615
Vancouver Style
YE H, Wang W, Ding Y, Liu X, Jia W, Luo W, et al. Spontaneous running wheel improves neuroprotection efficacy of ischemic postconditioning in mice following ischemia/reperfusion injury. BIOCELL . 2018;42(3):79-86 https://doi.org/10.32604/biocell.2018.04615
IEEE Style
H. YE et al., "Spontaneous running wheel improves neuroprotection efficacy of ischemic postconditioning in mice following ischemia/reperfusion injury," BIOCELL , vol. 42, no. 3, pp. 79-86. 2018. https://doi.org/10.32604/biocell.2018.04615

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cc Copyright © 2018 The Author(s). Published by Tech Science Press.
This work is licensed under a Creative Commons Attribution 4.0 International License , which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
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