Open Access
ARTICLE
Spontaneous running wheel improves neuroprotection efficacy of ischemic postconditioning in mice following ischemia/reperfusion injury
Hong YE1,6,#, WeiWei Wang2,#, Yu Ding3,#, XiaoLei Liu4,#, WenJI Jia1, WeiLi Luo1, HuiJuan Fan1, HongQun Zhou1, Jin Wang1, JianLong Ju1, DongMing Zhou7, TianHao Bao5,1,*, YuHong Zhu1,*
1
Department of Neurology, the Second Affiliated Hospital of Kunming Medical University, Kunming, China
2
Department of Cardiology, the Second Affiliated Hospital of Kunming Medical University, Kunming, China
3
Department of Ultrasound, the Second Affiliated Hospital of Kunming Medical University, Kunming, China
4
Department of Neurology, the First Affiliated Hospital of Kunming Medical University, Kunming, China
5
The Mental Health Center of Kunming Medical University, Kunming, China
6
Kunming Medical University, Kunming, China
7
Biomedical Engineering, University of Alberta, Edmonton, Alberta, Canada
# Hong Ye, Yu Ding, WeiWei Wang, XiaoLei Liu contributed equally to this article.
* Address correspondence to: TianHao Bao, ; YuHong Zhu,
BIOCELL 2018, 42(3), 79-86. https://doi.org/10.32604/biocell.2018.04615
Abstract
Ischemic postconditioning (IP) has been shown to provide protection for ischemia/reperfusion (IR)
injury, but its efficacy is limited. In this study we hypothesized that spontaneous running wheel (RW) could improve
neuroprotection efficacy of IP for IR. We established mouse models of IR and showed that compared to Sham group,
IR group had obvious brain infract and neurological dysfunction. In IR+IP group, brain infract and neurological
dysfunction improved compared to IR group. However, in IR+IP+RW group brain infract and neurological dysfunction
improved much better. TUNEL assay showed that IP but not RW significantly reduced the number of apoptotic cells
after IR. However, the number of apoptotic cells was significantly reduced in RW+IP group. In addition, the levels of
pro-apoptotic factors increased in IR group but significantly reduced in IR+IP+RW group, while the levels of antiapoptotic factors decreased in IR group but significantly increased in IR+IP+RW group. Moreover, in IR+IP+RW
group, MDA level was further decreased and SOD level was further increased compared to IR+IP group. Finally, both
PI3K inhibitor and STAT3 inhibitor significantly worsened brain infract and neurological dysfunction and promoted
apoptosis in IR mice. In conclusion, RW combined with IP reduces brain infract and neurological dysfunction in mice
after IR, and this is associated with enhanced anti-apoptotic and anti-oxidant benefits via the activation of PI3K and
STAT3 pathways.
Keywords
Cite This Article
YE, H., Wang, W., Ding, Y., Liu, X., Jia, W. et al. (2018). Spontaneous running wheel improves neuroprotection efficacy of ischemic postconditioning in mice following ischemia/reperfusion injury.
BIOCELL, 42(3), 79–86. https://doi.org/10.32604/biocell.2018.04615
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