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Nerve growth factor alleviates cerebral infarction and neurologic deficits by regulating VEGF, SDF-1 and S100A12 expression through PI3K pathway

Zheng LI1, §, Zhongle ZHAO2, §, Peng ZHANG3

1 Department of Neurosurgery, People’s Hospital of Deyang City, Deyang, China
2 Department of Emergency, the Forth People’s Hospital of Shaanxi, Xi’an, China
3 Department of Emergency, First People’s Hospital of Nantong, Nantong, China
§ These authors contributed equally to this work

* Address correspondence to: Zhongle Zhao, email

BIOCELL 2019, 43(3), 183-190. https://doi.org/ 10.32604/biocell.2019.06922

Abstract

Stroke remains the leading cause of death and disability worldwide, which destroys the quality of patients’ lives and thus is becoming a heavy burden to the society. However, the current therapeutic approaches are far from satisfaction. The objective of this study is to elucidate the impact of nerve growth factor (NGF) on the brain damage induced by cerebral ischemia and its potential molecular mechanism. Middle cerebral artery occlusion (MCAO) rats were used as animal models and neurological functions were evaluated by modified Neurological Severity Score (NSS). Brain cell apoptosis was analyzed by TUNEL-positive staining while brain infarct size was determined according to 2% 2,3,5-triphenyltetrazolium chloride (TCC) staining volume. Rats receiving NGF demonstrated significantly alleviated brain damage, reflected by a substantial improvement in the neurobehavioral outcome, a decrease in brain cell apoptosis and shrinkage of brain infarct volume. Further analysis revealed a markedly elevated circulating vascular endothelial growth factor (VEGF) and stromal cell-derived factor 1 (SDF-1) levels as well as a significant downregulation of SA10012 expression in NGF treated group compared with the untreated group. Strikingly, the protective effect of NGF on cerebral ischemic injury was abolished in rats treated with both NGF and PI3K inhibitors, indicating that phosphoinositide-3-kinase (PI3K) signaling is essential for NGF function. In conclusion, NGF treatment might be a potential therapeutic approach against cerebral infarction by downregulating SA10012 expression and upregulating VEGF, SDF-1 in a PI3K signaling dependent manner.

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LI, Z., ZHAO, Z., ZHANG, P. (2019). Nerve growth factor alleviates cerebral infarction and neurologic deficits by regulating VEGF, SDF-1 and S100A12 expression through PI3K pathway. BIOCELL, 43(3), 183–190.



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