Open Access
REVIEW
Bengü ERGÜDEN
BIOCELL, Vol.43, No.3, pp. 103-118, 2019, DOI:10.32604/biocell.2019.06517
Abstract The expression of a gene is governed at various levels, from transcriptional to translational level. The
translational control is widely used to regulate gene expression, especially when a rapid, local, and selective control over
protein synthesis is required. The present review describes instructive examples of translational regulation in yeast,
together with regulatory elements within mRNAs. The review also outlines the important contributions of mRNAbinding proteins that act in harmony with several translational elements to generate appropriate translational signals
and responses. More >
Open Access
REVIEW
Ji SHI; Peixin SUN, Ye ZHANG, Bing YAO, Haozhe PIAO
BIOCELL, Vol.43, No.3, pp. 119-128, 2019, DOI:10.32604/biocell.2019.07577
Abstract Glioma is the most common primary malignant brain tumor with a poor survival rate. In recent years, no
significant progress has been made in the treatment of gliomas in contrast to the development of improved diagnosis
via molecular typing. Newcastle disease virus (NDV), a negative-stranded RNA virus that exhibits oncolytic activity,
has been investigated for its capacity to elicit antitumor activity in many types of cancers, including glioma. Therefore,
application of oncolytic viruses, such as NDV, as a new treatment strategy to specifically target aberrant signaling in
glioblastomas has brought new hope. For many years, NDV has been investigated for… More >
Open Access
ARTICLE
Wen JING1,§, Chuanfen LI2,3,§, Yingli LU4, Lianshi FENG4
BIOCELL, Vol.43, No.3, pp. 129-138, 2019, DOI:10.32604/biocell.2019.06087
Abstract This study aimed to investigate the microRNA expression profile and the characteristics of lipid metabolism
in the livers of rats undergoing a high-fat diet. Fifty male Sprague-Dawley (SD) rats were divided into a standard chow
group (C group, N = 10) and a high-fat diet group (H group, N = 40). After 12 weeks, the rat body weight, body length,
fat mass, and serum lipid concentration were measured. The expression profile of microRNAs and the gene and protein
expression levels involved in lipid metabolism in rat liver were detected. Body fat and serum lipid concentrations
were all significantly higher in… More >
Open Access
ARTICLE
Dairong LI1, Xianlu ZHUO1,2, Lumi HUANG1, Xiaohui JI1, Donglin WANG1
BIOCELL, Vol.43, No.3, pp. 139-144, 2019, DOI:10.32604/biocell.2019.06460
Abstract X-ray repair cross-complementing protein 1 (XRCC1) could repair cisplatin-induced DNA damage. XRCC1
Arg399Gln and Arg194Trp variants alter XRCC1 expression and function, leading to changes in cancer sensitivity to
cisplatin treatment. This study aimed to investigate the effects of XRCC1 Arg399Gln and Arg194Trp polymorphisms
on cell viability, apoptosis and XRCC1 expression in cisplatin-sensitive A549 and cisplatin-resistant A549/DDP nonsmall cell lung cancer (NSCLC) cells. Plasmids carrying XRCC1 Arg399Gln and Arg194Trp were constructed and
transfected into A549 and A549/DDP cells. RT–PCR, Western blot, MTT assay, and flow cytometry analysis were
performed to assess cell viability, apoptosis, and XRCC1 expression. Compared to control cells,… More >
Open Access
ARTICLE
Zahra HOSSEININIA1, Sara SOLTANIAN2, Naser MAHDAVI-SHAHRI3, Hesam DEHGHANI1,4
BIOCELL, Vol.43, No.3, pp. 145-154, 2019, DOI:10.32604/biocell.2019.06468
Abstract Maintenance of pluripotency depends to diverse regulatory factors. Studies in embryonic stem cells
(ESCs) have indicated that large intergenic non-coding RNAs (lincRNAs) are involved in the regulatory network
of pluripotency. However, the presence and function of pluripotency-associated lincRNAs in cancer cells with
pluripotency features are unknown. In this study, we used embryonal carcinoma (EC) P19 cell lines to investigate the
expression level of Halr1 in pluripotency and retinoic acid (RA)-induced differentiated states. Down-regulation of
pluripotency associated factors such as OCT4, NANOG, SSEA1 and alkaline phosphatase at transcript and protein
levels were used to confirm the differentiated status of P19 cells.… More >
Open Access
ARTICLE
Mahmoud MOUSTAFA1,2 , Saad ALAMRI1, Tarek TAHA3, Ali SHATI1, Sulaiman ALRUMMAN1, Mohamed ALKAHTANI1
BIOCELL, Vol.43, No.3, pp. 155-166, 2019, DOI:10.32604/biocell.2019.06814
Abstract The aim of the current research was to clone and to characterize the partial 66 kDa streptavidin-binding
peptide (SBP) found in the germinated embryos of Pisum sativum L. var. Alaska. The pea (P. sativum var. Alaska)
embryos possess prominent 66 kDa SBPs that gradually disappeared after few hours of germination in germinated
embryos, but not in the cotyledons. The total RNA was isolated from embryos of P. sativum but could not be isolated
from the cotyledons. The partial nucleotides sequences of 66 kDa SBPs of embryonic stalk (P. sativum var. Alaska) were
cloned and identified using pMOSBlue vector. 66 kDa… More >
Open Access
ARTICLE
Atif Kamil1, Mubarak Ali Khan1, Muhammad AAsim2, Nadir Zaman Khan2, Raham Sher Khan1, Muhsin Jamal3, Waqar Ahmad4, Mir Azam Khan4, Fazal Jalil4
BIOCELL, Vol.43, No.3, pp. 167-174, 2019, DOI:10.32604/biocell.2019.06729
Abstract Several toxic compounds are known to induce apoptosis in mammalian cell lines. The human neuroblastoma
cells (SH-SY5Y) were exposed to the phosphatase inhibiting toxin okadaic acid (OA) or hydrogen peroxide (H2O2)
to induce apoptosis as well as generate reactive oxygen species (ROS). Mitoxantrone (MXT) was used as a positive
control for apoptosis. The SH-SY5Y cells were transfected with eukaryotic expression plasmid pHyPer-dMito encoding
mitochondrial-targeted fluorescent or pHyPer-dCito encoding cytoplasmic-targeted fluorescent sensor for hydrogen
peroxide (HyPer). The ERp57, also called GRP58 (Glucose-regulated protein 58), is a stress protein induced in
conditions like glucose starvation and viral infection. Recently ERp57 was shown… More >
Open Access
ARTICLE
Abdulkareem Younis SULEIMAN1, Intisar Salim PITY2, Mohammed R MOHAMMED2, Bashar Abduljabar HASSAWI2
BIOCELL, Vol.43, No.3, pp. 175-181, 2019, DOI:10.32604/biocell.2019.06884
Abstract Ovarian lesions are commonly encountered pathologies that cannot be categorized clinicoradiologically.
Definite diagnosis is of great importance for therapeutic and prognostic purposes. Histopathology gives accurate
diagnosis in most cases. Few cases need supportive tests like immunohistochemistry. Objective: to study the
histomorphological diversity of ovarian lesions, their age and location in North of Iraq (Mosul and Duhok). Patients
and methods: In the period extended from January 2008 to December 2011, 161 cases of ovarian lesions were
collected from pathology departments in Azadi General Hospital “Duhok” and Al-Jamhori Teaching Hospital “Mosul”.
Automated tissue processor was used for histologic study and Streptavidin-biotin method… More >
Open Access
ARTICLE
Zheng LI1, §, Zhongle ZHAO2, §, Peng ZHANG3
BIOCELL, Vol.43, No.3, pp. 183-190, 2019, DOI: 10.32604/biocell.2019.06922
Abstract Stroke remains the leading cause of death and disability worldwide, which destroys the quality of patients’
lives and thus is becoming a heavy burden to the society. However, the current therapeutic approaches are far from
satisfaction. The objective of this study is to elucidate the impact of nerve growth factor (NGF) on the brain damage
induced by cerebral ischemia and its potential molecular mechanism. Middle cerebral artery occlusion (MCAO) rats
were used as animal models and neurological functions were evaluated by modified Neurological Severity Score (NSS).
Brain cell apoptosis was analyzed by TUNEL-positive staining while brain infarct size was determined… More >
Open Access
ARTICLE
Tong MA1, §, Fafen WANG2, §, Xiaohui WANG1
BIOCELL, Vol.43, No.3, pp. 191-198, 2019, DOI:10.32604/biocell.2019.06989
Abstract Cervical cancer (CC), has been identified as one of the most frequent malignant tumors all over the world,
with high mortality in females. A growing number of investigations have confirmed that long noncoding RNAs
(lncRNAs) play a crucial role in the progression of multiple cancers. Nonetheless, the biological function and regulatory
mechanism of LINC01772 in CC haven’t been explored so far. In this study, LINC01772 expression was found to be
upregulated in tissues and cells of CC. Knocking down LINC01772 suppressed CC cell proliferation, migration and
epithelial-mesenchymal transition (EMT) process. Through molecular mechanism assays, LINC01772 was verified
to be bound… More >
Open Access
ARTICLE
Jie REN1, Qian LIANG2
BIOCELL, Vol.43, No.3, pp. 199-206, 2019, DOI: 10.32604/biocell.2019.07128
Abstract This study aimed to investigate the role of high mobility group box-1 (HMGB1) expression in oral squamous
cell carcinoma; HMGB1 promoted the proliferation and invasion of oral squamous cell carcinoma via activating
epithelial-mesenchymal transformation (EMT). In this study, RNA transfection was used to silence the expression of
HMGB1 in oral squamous cell carcinoma cells. CCK-8, cell clone formation and trans-well assays were used to detect
the proliferation and invasion of cells before and after HMGB1 silencing. qRT-PCR and Western blot were used to
detect changes in EMT marker protein expression before and after transfection. HMGB1 was significantly higher in
OSCC… More >
Open Access
ARTICLE
Chunhua YANG1,2,3, Ye TIAN1,2,3
BIOCELL, Vol.43, No.3, pp. 207-214, 2019, DOI:10.32604/biocell.2019.07258
Abstract Sperm-associated antigen 9 (SPAG9) expression is increased in prostate tissues of prostate cancer
patients. This experimental study aimed to investigate the role of SPAG9 in bone metastasis of prostate cancer.
Immunohistochemical analysis showed that SPAG9 staining was positive in 81.67% of 240 cases of prostatic carcinoma
but only in 6.67% of 120 cases of benign prostate hyperplasia. Strong PAG9 staining was positively correlated with
Gleason score and bone metastasis in 240 prostate cancer patients (p < 0.05), but not with the age or serum prostatespecific antigen level (p > 0.05). PC-3 cells were transfected with shRNA against SPAG9, and CCK-8… More >
Open Access
ARTICLE
Mohammad UBAIDILLAH1,2, Miswar FAPERTA1,2, Kyung-Min KIM1
BIOCELL, Vol.43, No.3, pp. 215-224, 2019, DOI:10.32604/biocell.2019.07549
Abstract Abiotic stresses, such as drought and salinity, adversely affect plant growth and productivity. Comparison
between non transgenic and transgenic rice harboring CaMsrB2 gene, which induces tolerance to abiotic stress, is
important to observe response of gene under abiotic stress. Phytohormone showed a tendency to increase under
the drought stress or salinity stress in the transgenic plant. RT-PCR analysis showed that gene expression and
phytohormone levels under abiotic stress, to be closely related. The CaMsrB2 gene is related to the expression of JA and
ABA hormones. Therefore, the level of expression of these genes and hormones was observed. The transcription levels… More >
Open Access
ARTICLE
Farshid GOLSHANI1, Barat Ali FAKHERI1, Mahmood SOLOUKI1, Nafiseh MAHDINEZHAD1, Majid Reza KIANI FERIZ2
BIOCELL, Vol.43, No.3, pp. 225-232, 2019, DOI:10.32604/biocell.2019.07547
Abstract ITS (Internal Transcription Spacer of nuclear ribosomal DNA) regions method was applied to identify and
study the phylogenetic relationships existing between 41 individuals of Crocus, including 8 species collected in Iran (4
crop and 8 wild individuals) and 16 species (2 crop and 27 wild individuals) already registered in GenBank (NCBI).
PCR amplifications were performed using the primer designed on the nucleotide sequence of nuclear region (ITS).
A total of 12 sequences were obtained and registered in NCBI database. In particular, 4 of these sequences were not
already present in the scientific library. Nucleotide polymorphic sites were counted for ITS… More >
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