Home / Journals / BIOCELL / Vol.43, No.3, 2019
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  • Open Access

    REVIEW

    mRNA-specific translational regulation in yeast

    Bengü ERGÜDEN
    BIOCELL, Vol.43, No.3, pp. 103-118, 2019, DOI:10.32604/biocell.2019.06517
    Abstract The expression of a gene is governed at various levels, from transcriptional to translational level. The translational control is widely used to regulate gene expression, especially when a rapid, local, and selective control over protein synthesis is required. The present review describes instructive examples of translational regulation in yeast, together with regulatory elements within mRNAs. The review also outlines the important contributions of mRNAbinding proteins that act in harmony with several translational elements to generate appropriate translational signals and responses. More >

  • Open Access

    REVIEW

    The antitumor effects of Newcastle disease virus on glioma

    Ji SHI; Peixin SUN, Ye ZHANG, Bing YAO, Haozhe PIAO
    BIOCELL, Vol.43, No.3, pp. 119-128, 2019, DOI:10.32604/biocell.2019.07577
    Abstract Glioma is the most common primary malignant brain tumor with a poor survival rate. In recent years, no significant progress has been made in the treatment of gliomas in contrast to the development of improved diagnosis via molecular typing. Newcastle disease virus (NDV), a negative-stranded RNA virus that exhibits oncolytic activity, has been investigated for its capacity to elicit antitumor activity in many types of cancers, including glioma. Therefore, application of oncolytic viruses, such as NDV, as a new treatment strategy to specifically target aberrant signaling in glioblastomas has brought new hope. For many years, NDV has been investigated for… More >

  • Open Access

    ARTICLE

    MicroRNA expression profile and lipid metabolism characteristics in liver of rat undergoing high-fat diet

    Wen JING1,§, Chuanfen LI2,3,§, Yingli LU4, Lianshi FENG4
    BIOCELL, Vol.43, No.3, pp. 129-138, 2019, DOI:10.32604/biocell.2019.06087
    Abstract This study aimed to investigate the microRNA expression profile and the characteristics of lipid metabolism in the livers of rats undergoing a high-fat diet. Fifty male Sprague-Dawley (SD) rats were divided into a standard chow group (C group, N = 10) and a high-fat diet group (H group, N = 40). After 12 weeks, the rat body weight, body length, fat mass, and serum lipid concentration were measured. The expression profile of microRNAs and the gene and protein expression levels involved in lipid metabolism in rat liver were detected. Body fat and serum lipid concentrations were all significantly higher in… More >

  • Open Access

    ARTICLE

    XRCC1 Arg399Gln and Arg194Trp polymorphisms regulate XRCC1 expression and chemoresistance of non-small cell lung cancer cells

    Dairong LI1, Xianlu ZHUO1,2, Lumi HUANG1, Xiaohui JI1, Donglin WANG1
    BIOCELL, Vol.43, No.3, pp. 139-144, 2019, DOI:10.32604/biocell.2019.06460
    Abstract X-ray repair cross-complementing protein 1 (XRCC1) could repair cisplatin-induced DNA damage. XRCC1 Arg399Gln and Arg194Trp variants alter XRCC1 expression and function, leading to changes in cancer sensitivity to cisplatin treatment. This study aimed to investigate the effects of XRCC1 Arg399Gln and Arg194Trp polymorphisms on cell viability, apoptosis and XRCC1 expression in cisplatin-sensitive A549 and cisplatin-resistant A549/DDP nonsmall cell lung cancer (NSCLC) cells. Plasmids carrying XRCC1 Arg399Gln and Arg194Trp were constructed and transfected into A549 and A549/DDP cells. RT–PCR, Western blot, MTT assay, and flow cytometry analysis were performed to assess cell viability, apoptosis, and XRCC1 expression. Compared to control cells,… More >

  • Open Access

    ARTICLE

    Down-regulation of Halr1 during induced differentiation of embryonal carcinoma P19 cells

    Zahra HOSSEININIA1, Sara SOLTANIAN2, Naser MAHDAVI-SHAHRI3, Hesam DEHGHANI1,4
    BIOCELL, Vol.43, No.3, pp. 145-154, 2019, DOI:10.32604/biocell.2019.06468
    Abstract Maintenance of pluripotency depends to diverse regulatory factors. Studies in embryonic stem cells (ESCs) have indicated that large intergenic non-coding RNAs (lincRNAs) are involved in the regulatory network of pluripotency. However, the presence and function of pluripotency-associated lincRNAs in cancer cells with pluripotency features are unknown. In this study, we used embryonal carcinoma (EC) P19 cell lines to investigate the expression level of Halr1 in pluripotency and retinoic acid (RA)-induced differentiated states. Down-regulation of pluripotency associated factors such as OCT4, NANOG, SSEA1 and alkaline phosphatase at transcript and protein levels were used to confirm the differentiated status of P19 cells.… More >

  • Open Access

    ARTICLE

    Cloning and characterization of 66 kDa streptavidin-binding peptides (SBP) of Pisum sativum L. embryo specific to var. Alaska

    Mahmoud MOUSTAFA1,2 , Saad ALAMRI1, Tarek TAHA3, Ali SHATI1, Sulaiman ALRUMMAN1, Mohamed ALKAHTANI1
    BIOCELL, Vol.43, No.3, pp. 155-166, 2019, DOI:10.32604/biocell.2019.06814
    Abstract The aim of the current research was to clone and to characterize the partial 66 kDa streptavidin-binding peptide (SBP) found in the germinated embryos of Pisum sativum L. var. Alaska. The pea (P. sativum var. Alaska) embryos possess prominent 66 kDa SBPs that gradually disappeared after few hours of germination in germinated embryos, but not in the cotyledons. The total RNA was isolated from embryos of P. sativum but could not be isolated from the cotyledons. The partial nucleotides sequences of 66 kDa SBPs of embryonic stalk (P. sativum var. Alaska) were cloned and identified using pMOSBlue vector. 66 kDa… More >

  • Open Access

    ARTICLE

    Detection of ROS and translocation of ERP-57 in apoptotic induced human neuroblastoma (SH-SY5Y) cells

    Atif Kamil1, Mubarak Ali Khan1, Muhammad AAsim2, Nadir Zaman Khan2, Raham Sher Khan1, Muhsin Jamal3, Waqar Ahmad4, Mir Azam Khan4, Fazal Jalil4
    BIOCELL, Vol.43, No.3, pp. 167-174, 2019, DOI:10.32604/biocell.2019.06729
    Abstract Several toxic compounds are known to induce apoptosis in mammalian cell lines. The human neuroblastoma cells (SH-SY5Y) were exposed to the phosphatase inhibiting toxin okadaic acid (OA) or hydrogen peroxide (H2O2) to induce apoptosis as well as generate reactive oxygen species (ROS). Mitoxantrone (MXT) was used as a positive control for apoptosis. The SH-SY5Y cells were transfected with eukaryotic expression plasmid pHyPer-dMito encoding mitochondrial-targeted fluorescent or pHyPer-dCito encoding cytoplasmic-targeted fluorescent sensor for hydrogen peroxide (HyPer). The ERp57, also called GRP58 (Glucose-regulated protein 58), is a stress protein induced in conditions like glucose starvation and viral infection. Recently ERp57 was shown… More >

  • Open Access

    ARTICLE

    Histopathological patterns of ovarian lesions: A study of 161 cases

    Abdulkareem Younis SULEIMAN1, Intisar Salim PITY2, Mohammed R MOHAMMED2, Bashar Abduljabar HASSAWI2
    BIOCELL, Vol.43, No.3, pp. 175-181, 2019, DOI:10.32604/biocell.2019.06884
    Abstract Ovarian lesions are commonly encountered pathologies that cannot be categorized clinicoradiologically. Definite diagnosis is of great importance for therapeutic and prognostic purposes. Histopathology gives accurate diagnosis in most cases. Few cases need supportive tests like immunohistochemistry. Objective: to study the histomorphological diversity of ovarian lesions, their age and location in North of Iraq (Mosul and Duhok). Patients and methods: In the period extended from January 2008 to December 2011, 161 cases of ovarian lesions were collected from pathology departments in Azadi General Hospital “Duhok” and Al-Jamhori Teaching Hospital “Mosul”. Automated tissue processor was used for histologic study and Streptavidin-biotin method… More >

  • Open Access

    ARTICLE

    Nerve growth factor alleviates cerebral infarction and neurologic deficits by regulating VEGF, SDF-1 and S100A12 expression through PI3K pathway

    Zheng LI1, §, Zhongle ZHAO2, §, Peng ZHANG3
    BIOCELL, Vol.43, No.3, pp. 183-190, 2019, DOI: 10.32604/biocell.2019.06922
    Abstract Stroke remains the leading cause of death and disability worldwide, which destroys the quality of patients’ lives and thus is becoming a heavy burden to the society. However, the current therapeutic approaches are far from satisfaction. The objective of this study is to elucidate the impact of nerve growth factor (NGF) on the brain damage induced by cerebral ischemia and its potential molecular mechanism. Middle cerebral artery occlusion (MCAO) rats were used as animal models and neurological functions were evaluated by modified Neurological Severity Score (NSS). Brain cell apoptosis was analyzed by TUNEL-positive staining while brain infarct size was determined… More >

  • Open Access

    ARTICLE

    LncRNA LINC01772 promotes metastasis and EMT process in cervical cancer by sponging miR-3611 to relieve ZEB1

    Tong MA1, §, Fafen WANG2, §, Xiaohui WANG1
    BIOCELL, Vol.43, No.3, pp. 191-198, 2019, DOI:10.32604/biocell.2019.06989
    Abstract Cervical cancer (CC), has been identified as one of the most frequent malignant tumors all over the world, with high mortality in females. A growing number of investigations have confirmed that long noncoding RNAs (lncRNAs) play a crucial role in the progression of multiple cancers. Nonetheless, the biological function and regulatory mechanism of LINC01772 in CC haven’t been explored so far. In this study, LINC01772 expression was found to be upregulated in tissues and cells of CC. Knocking down LINC01772 suppressed CC cell proliferation, migration and epithelial-mesenchymal transition (EMT) process. Through molecular mechanism assays, LINC01772 was verified to be bound… More >

  • Open Access

    ARTICLE

    HMGB1 promotes the proliferation and invasion of oral squamous cell carcinoma via activating epithelial-mesenchymal transformation

    Jie REN1, Qian LIANG2
    BIOCELL, Vol.43, No.3, pp. 199-206, 2019, DOI: 10.32604/biocell.2019.07128
    Abstract This study aimed to investigate the role of high mobility group box-1 (HMGB1) expression in oral squamous cell carcinoma; HMGB1 promoted the proliferation and invasion of oral squamous cell carcinoma via activating epithelial-mesenchymal transformation (EMT). In this study, RNA transfection was used to silence the expression of HMGB1 in oral squamous cell carcinoma cells. CCK-8, cell clone formation and trans-well assays were used to detect the proliferation and invasion of cells before and after HMGB1 silencing. qRT-PCR and Western blot were used to detect changes in EMT marker protein expression before and after transfection. HMGB1 was significantly higher in OSCC… More >

  • Open Access

    ARTICLE

    SPAG9 promotes prostate cancer growth and metastasis

    Chunhua YANG1,2,3, Ye TIAN1,2,3
    BIOCELL, Vol.43, No.3, pp. 207-214, 2019, DOI:10.32604/biocell.2019.07258
    Abstract Sperm-associated antigen 9 (SPAG9) expression is increased in prostate tissues of prostate cancer patients. This experimental study aimed to investigate the role of SPAG9 in bone metastasis of prostate cancer. Immunohistochemical analysis showed that SPAG9 staining was positive in 81.67% of 240 cases of prostatic carcinoma but only in 6.67% of 120 cases of benign prostate hyperplasia. Strong PAG9 staining was positively correlated with Gleason score and bone metastasis in 240 prostate cancer patients (p < 0.05), but not with the age or serum prostatespecific antigen level (p > 0.05). PC-3 cells were transfected with shRNA against SPAG9, and CCK-8… More >

  • Open Access

    ARTICLE

    Identification of phytohormone changes and its related genes under abiotic stresses in transgenic rice

    Mohammad UBAIDILLAH1,2, Miswar FAPERTA1,2, Kyung-Min KIM1
    BIOCELL, Vol.43, No.3, pp. 215-224, 2019, DOI:10.32604/biocell.2019.07549
    Abstract Abiotic stresses, such as drought and salinity, adversely affect plant growth and productivity. Comparison between non transgenic and transgenic rice harboring CaMsrB2 gene, which induces tolerance to abiotic stress, is important to observe response of gene under abiotic stress. Phytohormone showed a tendency to increase under the drought stress or salinity stress in the transgenic plant. RT-PCR analysis showed that gene expression and phytohormone levels under abiotic stress, to be closely related. The CaMsrB2 gene is related to the expression of JA and ABA hormones. Therefore, the level of expression of these genes and hormones was observed. The transcription levels… More >

  • Open Access

    ARTICLE

    Study of phylogenic relationships of some wild and crop species of Iranian Crocus by ITS nuclear loci

    Farshid GOLSHANI1, Barat Ali FAKHERI1, Mahmood SOLOUKI1, Nafiseh MAHDINEZHAD1, Majid Reza KIANI FERIZ2
    BIOCELL, Vol.43, No.3, pp. 225-232, 2019, DOI:10.32604/biocell.2019.07547
    Abstract ITS (Internal Transcription Spacer of nuclear ribosomal DNA) regions method was applied to identify and study the phylogenetic relationships existing between 41 individuals of Crocus, including 8 species collected in Iran (4 crop and 8 wild individuals) and 16 species (2 crop and 27 wild individuals) already registered in GenBank (NCBI). PCR amplifications were performed using the primer designed on the nucleotide sequence of nuclear region (ITS). A total of 12 sequences were obtained and registered in NCBI database. In particular, 4 of these sequences were not already present in the scientific library. Nucleotide polymorphic sites were counted for ITS… More >

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