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YANGYI HE^1,2, DAVID SANCHEZ-LORENTE^3,4,5, MELISSA ACOSTA-PLASENCIA¹, MARC BOADA^3,4,5, ANGELA GUIRAO^3,4,5, RAMON M. MARRADES^4,5,6,7, LAUREANO MOLINS^3,4,5, ALFONS NAVARRO^1,4,5,*
BIOCELL, Vol.47, No.5, pp. 951-957, 2023, DOI:10.32604/biocell.2023.027718
Abstract Tumor-secreted extracellular vesicles (EVs) participate in the metastasis process through different mechanisms, including the preparation of the pre-metastatic niche to grant circulating tumor cells (CTCs) implantation and growth. The study of the metastasis process through the analysis of CTCs and tumor-derived EVs is difficult because of the dilution grade of these elements in peripheral blood. In early-stage lung cancer patients, the tumor-secreted products are even more diluted. An attractive strategy in surgical lung cancer patients is to purify them from a pulmonary tumor-draining vein where they are enriched. The information obtained from the analysis of EVs and CTCs purified from… More >

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ADEM OZCELIK^1,*, OZGE CEVIK²
BIOCELL, Vol.47, No.5, pp. 959-964, 2023, DOI:10.32604/biocell.2023.028371
Abstract Exosomes are important biomarkers for clinical diagnosis. It is critical to isolate secreted exosomes from bodily fluids such as blood, saliva, breast milk, and urine for liquid biopsy applications. The field of microfluidics provides numerous benefits for biosample processing, diagnostics, and prognostics. Several microfluidics-based methods have been employed for the isolation and purification of exosomes in the last ten years. These microfluidic methods can be grouped into two categories based on passive and active isolation mechanisms. In the first group, inertial and hydrodynamic forces are employed to separate exosomes based on their size differences. In the second group, external forcefields… More >

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YINGHUA LUO^1,#, YU ZHANG^2,#, TONG ZHANG^2,#, YANNAN LI², HUI XUE², JINGLONG CAO², WENSHUANG HOU², JIAN LIU², YUHE CUI², TING XU², CHENGHAO JIN^2,3,*
BIOCELL, Vol.47, No.5, pp. 965-975, 2023, DOI:10.32604/biocell.2023.027729
Abstract Marine collagen peptides (MCPs) are natural products prepared by hydrolyzing marine collagen protein through a variety of chemical methods or enzymes. MCPs have a range of structures and biological activities and are widely present in marine species. MCPs also have a small molecular weight, are easily modified, and absorbed by the body. These properties have attracted great interest from researchers studying antioxidant, anti-tumor, and anti-aging activities. MCPs of specific molecular weights have significant anti-tumor activity and no toxic side effects. Thus, MCPs have the potential use as anti-cancer adjuvant drugs. Free radicals produced by oxidation are closely related to human… More >

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MAN REN^1,2,3,#, YUAN YANG^1,2,#, DAN LI⁴, NANNAN ZHAO⁵, YUPING WANG^2,6,*, YONGNING ZHOU^2,6,*
BIOCELL, Vol.47, No.5, pp. 977-989, 2023, DOI:10.32604/biocell.2023.028402
Abstract Background: 1,2,3,4,6-penta-O-galloyl-beta-D-glucose (PGG) is a natural polyphenolic compound derived from multiple medicinal plants with favorable anticancer activity. Methods: In this study, the mechanisms of PGG against gastric cancer were explored through network pharmacology and molecular docking. First, the targets of PGG were searched in the Herbal Ingredients’ Targets (HIT), Similarity Ensemble Approach (SEA), and Super-PRED databases. The potential targets related to gastric cancer were predicted from the Human Gene Database (GeneCards) and DisGeNET databases. The intersecting targets of PGG and gastric cancer were obtained by Venn diagram and then subjected to protein-protein interaction analysis to screen hub targets. Functional and… More >

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YI WANG^1,#, SHANGQI CHEN^2,#, PENGCHENG YU¹, ZHEHAN BAO¹, CAN HU¹, YUHANG XIA¹, RUOLAN ZHANG¹, LI YUAN^3,4, HUA RUAN^5,*, JIANCHENG SUN^6,*
BIOCELL, Vol.47, No.5, pp. 991-1002, 2023, DOI:10.32604/biocell.2023.028587
Abstract Aim: Scavenger receptor class B, type I (SR-BI) is an integral plasma membrane protein that has been reported to be overexpressed in various malignancies, such as renal cancer, breast cancer, and prostate cancer, and is an independent prognostic factor. However, the clinical value and expression of SR-BI in GC are unknown. Our research aimed to explore the role of SR-BI in combination with immune markers as a diagnostic and prognostic marker for gastric cancer (GC). Methods: GC tissues, paracancerous tissues, and clinicopathological data of 149 patients were collected. The expression level of SR-BI, Tumor-infiltrating lymphocytes (TILs), and PD-L1 were evaluated… More >

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447

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SHA LI^1,2,#, YAQIONG LIU^3,#, CHAOLING YAO¹, ANJI XU¹, XIAOLING ZENG⁴, YUXIN GE⁴, XIAOWU SHENG⁴, HAILIN ZHANG^1,2, XIAO ZHOU^1,2,*, YING LONG^1,2,*
BIOCELL, Vol.47, No.5, pp. 1003-1019, 2023, DOI:10.32604/biocell.2023.027209
Abstract Background: Nuclear receptor binding SET domain protein-3 (NSD3) is a histone lysine methyltransferase and a crucial regulator of carcinogenesis in several cancers. We aimed to investigate the prognostic value and potential function of NSD3 in 33 types of human cancer. Methods: The data were obtained from The Cancer Genome Atlas. Kaplan-Meier analysis, CIBERSORT, gene set enrichment analysis, and gene set variation analysis were performed. The expression of NSD3 was measured using quantitative real-time polymerase chain reaction and western blot. Results: The expression of NSD3 was altered in pan-cancer samples. Patients with higher levels of NDS3 generally had shorter overall survival… More >

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XI YANG^1,5,#, HUIXIAN WU^2,3,#, CHAO XIONG^4,#, BO ZHAO¹, MEILIAN LIU¹, JIE QIN⁶, MEI DENG^1,2,3,*
BIOCELL, Vol.47, No.5, pp. 1021-1037, 2023, DOI:10.32604/biocell.2023.027103
Abstract Background: Cytochrome b561 (CYB561) plays a critical role in neuroendocrine function, cardiovascular regulation, and tumor growth; however, the prognostic value of CYB561 in patients with breast cancer and the relationship between CYB561 expression and immune infiltration in breast cancer remain unclear. Methods: The mRNA expression and clinical data of patients with breast cancer were obtained from The Cancer Genome Atlas database. Functional enrichment analysis was used to explore underlying biological functions associated with CYB561. The methylation status of CYB561 was analyzed using the MethSurv database. The enrichment score of immune cell infiltration for CYB561 in breast cancer was calculated using… More >

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456

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QIAOLING WU^1,2, ZHAOLEI CUI³, HONGMEI XIA¹, SHAN JIANG¹, JING BAI⁴, ZHUO SHAO⁴, YANG SUN^1,*
BIOCELL, Vol.47, No.5, pp. 1039-1050, 2023, DOI:10.32604/biocell.2023.027494
Abstract Background: Ovarian cancer (OC) is a leading cause of gynecological cancer-linked deaths worldwide. Exosomal miR-1825 and its target gene C-type lectin domain family 5 member A (CLEC5A) are associated with tumorigenesis in cancers that was further probed. Methods: Exosomal miR-1825 expression in exosomes and its impact on overall survival (OS) prediction were determined using Gene Expression Omnibus (GEO) and The Cancer Genome Atlas (TCGA) data. Target genes of miR-1825 were searched in five prediction databases and prognostically significant differentially expressed genes were identified. Gene Ontology (GO) and Kyoto Encyclopedia of Genes and Genomes (KEGG) enrichment analyses were carried out. The… More >

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445

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OMER ERDOGAN¹, GULEN MELIKE DEMIRBOLAT², OZGE CEVIK^1,*
BIOCELL, Vol.47, No.5, pp. 1051-1063, 2023, DOI:10.32604/biocell.2023.028129
Abstract Background: Raloxifene, a selective estrogen receptor modulator, is also known to be a lysosomotropic agent. The bioavailability of raloxifene is around 2% due to extensive hepatic transport. Exosomes are nanosized vesicles that are naturally released from cells. Method: In this study, exosomes released from HeLa cervical cancer cells were loaded with raloxifene to increase its bioavailability, and an aptamer was attached to the exosome membrane for targeting only HeLa cells. Characterization of exosomes isolated from HeLa cells was performed by transmission electron microscopy, zeta sizer, and western blotting. In addition, the cytotoxic, apoptotic, autophagic, and lysosomotropic effects of the prepared… More >

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LIHUA CHEN^1,2,#, HONGTIAN ZHANG^1,2,#, YONG XIA^1,2, KAI SUN¹, WENJIN CHEN¹, RUXIANG XU^1,2,*
BIOCELL, Vol.47, No.5, pp. 1065-1073, 2023, DOI:10.32604/biocell.2023.025924
Abstract Purpose: The present study summarized cases of children (n = 32) with medulloblastoma (MB) who were treated using stratified therapy based on risk grading and also discussed the factors affecting prognosis. Methods: According to the risk stratification criteria, the cases were divided into the following four risk groups: low, standard, high, and very high. The 5-year overall survival (OS) and progression-free survival (PFS) rates were summarized. Further, the effects on the prognosis of tumor size, tumor stage, degree of resection, treatment mode, metastatic recurrence, molecular typing, and risk stratification were analyzed. Results: In the present study, following surgery, 3 cases… More >

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ZHENG CAO^1,#, CHUNXIAO ZHOU^1,#, ZHIMIN WU¹, CHUNYAN WU¹, WEN ZHANG¹, SHILV CHEN¹, XINDONG ZHAO¹, SHAOLING WU^2,*
BIOCELL, Vol.47, No.5, pp. 1075-1083, 2023, DOI:10.32604/biocell.2023.027027
Abstract Background: Dihydroartemisinin (DHA) is reported to be a potential anticancer agent, and the mechanisms underlying the effects of DHA on diffuse large B cell lymphoma however are still obscure. This study aimed to assess the antitumor effect of DHA on diffuse large B cell lymphoma cells and to determine the potential underlying mechanisms of DHA-induced cell apoptosis. Methods: Here, the Cell Counting Kit 8 assay was conducted to study cell proliferation. We performed Annexin V-FITC/propidium iodide staining, real-time polymerase chain reaction, and western blot analysis to analyze cell apoptosis and potential molecular mechanisms. Results: The results showed that DHA substantially… More >

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XIN TIAN, XIANGLING HE^*, SHUQIN QIAN, RUNYING ZOU, KEKE CHEN, CHENGGUANG ZHU, ZEXI YIN
BIOCELL, Vol.47, No.5, pp. 1085-1093, 2023, DOI:10.32604/biocell.2023.027090
Abstract Background: The immunomodulatory effects of mesenchymal stem cells (MSCs) and their exosomes have been receiving increasing attention. This study investigated the immunoregulatory effects of human amniotic mesenchymal stem cells (hAMSCs) and their exosomes on phytohemagglutinin (PHA)-induced peripheral blood mononuclear cells (PBMCs). Methods: The hAMSCs used in the experiment were identified by light microscopy and flow cytometry, and the differentiation ability of the cells was determined by Oil Red O and Alizarin Red staining. The expressions of transforming growth factor (TGF)-β, indoleamine 2,3-dioxygenase (IDO), cyclooxygenase-2 (COX-2), hepatocyte growth factor (HGF), and interleukin (IL)-6 were detected by quantitative real-time polymerase chain reaction… More >

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MIZUNA YANO¹, KOTA HIROI¹, TETSUYA YUASA¹, KENJI INOUE¹, OSAMU YAMAMOTO¹, TAKAO NAKAMURA², DAISUKE SATO¹, ZHONGGANG FENG^1,*
BIOCELL, Vol.47, No.5, pp. 1095-1106, 2023, DOI:10.32604/biocell.2023.028186
Abstract Background: Human heart changes its energetic substrates from lactate and glucose to fatty acids during the neonatal period. Noticing the lack of fatty acids in media for the culture of cardiomyocytes derived from human pluripotent stem cells (hiPS-CM), researchers have supplemented mixtures of fatty acids to hiPS-CM and reported the enhancement in the maturation of hiPS-CM. In our previous studies, we separately supplemented two polyunsaturated fatty acids (PUFAs), docosahexaenoic acid (DHA) or arachidonic acid (AA), to rat fetal cardiomyocytes and found that the supplementations upregulated the expressions of mRNAs for cardiomyocyte differentiation, fatty acid metabolism, and cellular adhesion. The enhancement… More >

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476

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HAN WANG^1,2,#, YANG SUN^1,2,#, XIAOXU ZHANG^2,3, XIAOYING WANG⁴, YUJUN XIA^1,*, LISHENG WANG^1,2,*
BIOCELL, Vol.47, No.5, pp. 1107-1115, 2023, DOI:10.32604/biocell.2023.027131
Abstract Background: Inflammatory bowel disease (IBD) is a chronic inflammatory disease of the gastrointestinal tract. The destruction of the intestinal epithelial barrier is one of the major pathological processes in IBD pathology. Growing evidence indicated that epithelial cell ferroptosis is linked to IBD and is considered a target process. Methods: RAS-selective lethal 3 (RSL3) was used to induce ferroptosis in intestinal epithelial cell line No. 6 (IEC-6) cells, and cell ferroptosis and the effects of tanshinone IIA (Tan IIA) were determined by cell counting kit-8 (CCK-8), reactive oxygen species (ROS) staining, Giemsa staining and transmission electron microscope (TEM). The cell viability… More >

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XIANGCHAO DING^1,#, YANG ZHAO^2,#, XINGHUA ZHANG¹, HUIQING LIN^1,*
BIOCELL, Vol.47, No.5, pp. 1117-1126, 2023, DOI:10.32604/biocell.2023.026715
Abstract Background: Long-chain non-coding RNA (lncRNA) LINC00609 is a potential tumor suppressor, but the mechanism of action in non-small cell lung cancer (NSCLC) is yet to be understood.Objectives: The effects of LINC00609 on A549 cell proliferation, apoptosis, and cell cycle arrest were investigated. Methods: The LINC00609 levels in NSCLC and normal tissues were analyzed by bioinformatics. Expressions of LINC00609, miR-128-3p, and Rho family GTPase 3 (RND3) in NSCLC cells (A549) were determined by qRT-PCR. Bioinformatics analysis predicted target genes and dual-luciferase reporter assays to ensure that LINC00609 targeted miR-128-3p and miR-128-3p targeted RND3. The proliferation of cells was determined using EDU… More >

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WENQING LI¹, WANLI LI^2,3, BEI WANG¹, LIN ZHANG¹, SHENGLI LI³, CHENWAN LI¹, PINHUI WU¹, GUOQING YANG¹, GUOZHI ZHANG^1,*
BIOCELL, Vol.47, No.5, pp. 1127-1134, 2023, DOI:10.32604/biocell.2023.027863
（This article belongs to the Special Issue: )
Abstract Background: The selection of Marek’s disease (MD)-resistant breeds in Partridge Shank chicken, a popular local chicken breed in Henan Province of China, has practical value. We hypothesized that the two polymorphic sites (rs14527240 located in SMOC1 and GGaluGA156129 located in PTPN3) related to MD resistance in White Leghorn chickens are also applicable to Partridge Shank chickens. Methods: In this experiment, we screened 10 live hens and 2 live roosters with the double GG genotype by genotyping the two sites from 6500 Partridge Shank chickens. Nineteen one-day-old chicks with the double GG genotype were obtained by artificial insemination. Seventy-two one-day-old chickens… More >

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396

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ANA CECILIA LIAUDAT, FRANCISCA EBEL, BIANCA ANA OPIZZO, MARINA AURORA GONZALEZ, DAMIÁN BLOIS, PABLO BOSCH^#, NANCY RODRIGUEZ^#,*
BIOCELL, Vol.47, No.5, pp. 1135-1143, 2023, DOI:10.32604/biocell.2023.027628
Abstract Background: Capacitation is a set of physiological changes sperms undergo to acquire fertilizing capacity. In vivo, this process is directly associated with high calcium levels in sperm cytoplasm. Calcitriol, the vitamin D hypercalcemic metabolite, is related to human sperm motility, capacitation, and acrosome reaction. This work aimed to study the effect of calcitriol on bull sperm quality parameters and capacitation. Methods: One million freeze-thawed spermatozoa were obtained from different bulls and treated with 20 nM of calcitriol for 30 min. Untreated cells (negative control) and treated ones with calcitriol or heparin (100 µg/mL, positive capacitation control) were evaluated for motility,… More >

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QINGCHEN LUO^1,#,*, JIALU FENG^2,#, XIUQI DENG¹
BIOCELL, Vol.47, No.5, pp. 1145-1158, 2023, DOI:10.32604/biocell.2023.027847
Abstract As sessile organisms, plants possess a complex system to cope with environmental changes. Ca²⁺ functions as a vital second messenger in the stress signaling of plants, and the CBL-interacting protein kinases (CIPKs) serve as essential elements in the plant Ca²⁺ signaling pathway. In this study, calcineurin B-like protein-interacting protein kinase 26 (BdCIPK26) from Brachypodium distachyon was characterized. Overexpression of BdCIPK26 enhanced tolerance to drought and salt stress of transgenic plants. Further investigations revealed that BdCIPK26 participated in abscisic acid (ABA) signaling, conferred hypersensitivity to exogenous ABA in transgenic plants, and promoted endogenous ABA biosynthesis. Moreover, BdCIPK26 was found to maintain… More >

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479

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VIJAY KUMAR^1,#, PRAVEEN C. RAMAMURTHY^2,#, SIMRANJEET SINGH^2,#, DALJEET SINGH DHANJAL³, PARUL PARIHAR⁴, DEEPIKA BHATIA⁵, RAM PRASAD^6,*, JOGINDER SINGH^7,*
BIOCELL, Vol.47, No.5, pp. 1159-1176, 2023, DOI:10.32604/biocell.2023.022065
Abstract Phyllanthus emblica or Indian gooseberry is an integrated part of Ayurvedic and Traditional Chinese Medicines. For several decades, the well-known ancient herb has been extensively utilized in traditional medicine to cure diseases like fever, diabetes, constipation, jaundice, ulcers, biliousness, anemia, anorexia, and dyspepsia. In the traditional system, Indian gooseberry has various ethnomedicinal applications. In the Ayurvedic system, different methods of administration (anupan) have shown different ethnomedicinal properties of Indian gooseberry. Seventy well-known chemical components in Indian gooseberry have been identified through phytochemical evaluation, among which the flavonoids and phenols are most prominent. From the toxicity perspective, it is considered a… More >

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PAULA MARIELA GONZÁLEZ^1,2, JOAQUIN CABRERA^1,2, SUSANA PUNTARULO^1,2,*
BIOCELL, Vol.47, No.5, pp. 1177-1189, 2023, DOI:10.32604/biocell.2023.027107
Abstract

The purpose of this review is to briefly summarize the central role of iron (Fe) in terms of cellular alterations of the oxidative/protective balance with special emphasis on its possible involvement in ferroptosis-dependent disruption in aquatic organisms. In ferroptotic cells or tissues, the intracellular Fe level increases; meanwhile the treatment with Fe chelators limits ferroptosis. Eukaryotic algae can assimilate Fe from the environment through several mechanisms, and aquatic animals incorporate dissolved Fe and Fe bound to both inorganic particles and organic matter. The central role of lipid peroxidation mediating ferroptosis was demonstrated in some algae where both low and high… More >

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