Home / Journals / BIOCELL / Vol.47, No.5, 2023
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Artistic description of how we can obtain blood enriched in tumor secreted products by puncturing the pulmonary vein branch draining from the pulmonary segment where the lung tumor is located. The whole process is performed during the surgical resection of the lung cancer. The tumor-draining vein blood is enriched in tumor-derived products in comparison with the peripheral vein where they become highly diluted. Therefore tumor-draining vein analysis facilitates the study of circulating tumor cells (CTCs), extracellular vesicles, cfDNA or non-coding RNAs secreted by the tumor. There is no doubt of that it is an excellent source of tumor-derived products especially in early-stage patients treated with surgery.

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  • Open AccessOpen Access

    VIEWPOINT

    Analysis of tumor-draining vein secretome: A direct access to tumor-derived extracellular vesicles in surgical lung cancer patients

    YANGYI HE1,2, DAVID SANCHEZ-LORENTE3,4,5, MELISSA ACOSTA-PLASENCIA1, MARC BOADA3,4,5, ANGELA GUIRAO3,4,5, RAMON M. MARRADES4,5,6,7, LAUREANO MOLINS3,4,5, ALFONS NAVARRO1,4,5,*
    BIOCELL, Vol.47, No.5, pp. 951-957, 2023, DOI:10.32604/biocell.2023.027718
    Abstract Tumor-secreted extracellular vesicles (EVs) participate in the metastasis process through different mechanisms, including the preparation of the pre-metastatic niche to grant circulating tumor cells (CTCs) implantation and growth. The study of the metastasis process through the analysis of CTCs and tumor-derived EVs is difficult because of the dilution grade of these elements in peripheral blood. In early-stage lung cancer patients, the tumor-secreted products are even more diluted. An attractive strategy in surgical lung cancer patients is to purify them from a pulmonary tumor-draining vein where they are enriched. The information obtained from the analysis of EVs and CTCs purified from… More >

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    VIEWPOINT

    Microfluidic methods used in exosome isolation

    ADEM OZCELIK1,*, OZGE CEVIK2
    BIOCELL, Vol.47, No.5, pp. 959-964, 2023, DOI:10.32604/biocell.2023.028371
    Abstract Exosomes are important biomarkers for clinical diagnosis. It is critical to isolate secreted exosomes from bodily fluids such as blood, saliva, breast milk, and urine for liquid biopsy applications. The field of microfluidics provides numerous benefits for biosample processing, diagnostics, and prognostics. Several microfluidics-based methods have been employed for the isolation and purification of exosomes in the last ten years. These microfluidic methods can be grouped into two categories based on passive and active isolation mechanisms. In the first group, inertial and hydrodynamic forces are employed to separate exosomes based on their size differences. In the second group, external forcefields… More >

  • Open AccessOpen Access

    REVIEW

    Review on marine collagen peptides induce cancer cell apoptosis, necrosis, and autophagy by reducing oxidized free radicals

    YINGHUA LUO1,#, YU ZHANG2,#, TONG ZHANG2,#, YANNAN LI2, HUI XUE2, JINGLONG CAO2, WENSHUANG HOU2, JIAN LIU2, YUHE CUI2, TING XU2, CHENGHAO JIN2,3,*
    BIOCELL, Vol.47, No.5, pp. 965-975, 2023, DOI:10.32604/biocell.2023.027729
    Abstract Marine collagen peptides (MCPs) are natural products prepared by hydrolyzing marine collagen protein through a variety of chemical methods or enzymes. MCPs have a range of structures and biological activities and are widely present in marine species. MCPs also have a small molecular weight, are easily modified, and absorbed by the body. These properties have attracted great interest from researchers studying antioxidant, anti-tumor, and anti-aging activities. MCPs of specific molecular weights have significant anti-tumor activity and no toxic side effects. Thus, MCPs have the potential use as anti-cancer adjuvant drugs. Free radicals produced by oxidation are closely related to human… More >

  • Open AccessOpen Access

    ARTICLE

    Network pharmacology and molecular docking identify mechanisms of medicinal plant-derived 1,2,3,4,6-penta-O-galloyl-beta-D-glucose treating gastric cancer

    MAN REN1,2,3,#, YUAN YANG1,2,#, DAN LI4, NANNAN ZHAO5, YUPING WANG2,6,*, YONGNING ZHOU2,6,*
    BIOCELL, Vol.47, No.5, pp. 977-989, 2023, DOI:10.32604/biocell.2023.028402
    Abstract Background: 1,2,3,4,6-penta-O-galloyl-beta-D-glucose (PGG) is a natural polyphenolic compound derived from multiple medicinal plants with favorable anticancer activity. Methods: In this study, the mechanisms of PGG against gastric cancer were explored through network pharmacology and molecular docking. First, the targets of PGG were searched in the Herbal Ingredients’ Targets (HIT), Similarity Ensemble Approach (SEA), and Super-PRED databases. The potential targets related to gastric cancer were predicted from the Human Gene Database (GeneCards) and DisGeNET databases. The intersecting targets of PGG and gastric cancer were obtained by Venn diagram and then subjected to protein-protein interaction analysis to screen hub targets. Functional and… More >

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    ARTICLE

    SR-BI expression regulates the gastric cancer tumor immune microenvironment and is associated with poor prognosis

    YI WANG1,#, SHANGQI CHEN2,#, PENGCHENG YU1, ZHEHAN BAO1, CAN HU1, YUHANG XIA1, RUOLAN ZHANG1, LI YUAN3,4, HUA RUAN5,*, JIANCHENG SUN6,*
    BIOCELL, Vol.47, No.5, pp. 991-1002, 2023, DOI:10.32604/biocell.2023.028587
    Abstract Aim: Scavenger receptor class B, type I (SR-BI) is an integral plasma membrane protein that has been reported to be overexpressed in various malignancies, such as renal cancer, breast cancer, and prostate cancer, and is an independent prognostic factor. However, the clinical value and expression of SR-BI in GC are unknown. Our research aimed to explore the role of SR-BI in combination with immune markers as a diagnostic and prognostic marker for gastric cancer (GC). Methods: GC tissues, paracancerous tissues, and clinicopathological data of 149 patients were collected. The expression level of SR-BI, Tumor-infiltrating lymphocytes (TILs), and PD-L1 were evaluated… More >

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    ARTICLE

    Prognostic prediction and expression validation of NSD3 in pan-cancer analyses

    SHA LI1,2,#, YAQIONG LIU3,#, CHAOLING YAO1, ANJI XU1, XIAOLING ZENG4, YUXIN GE4, XIAOWU SHENG4, HAILIN ZHANG1,2, XIAO ZHOU1,2,*, YING LONG1,2,*
    BIOCELL, Vol.47, No.5, pp. 1003-1019, 2023, DOI:10.32604/biocell.2023.027209
    Abstract Background: Nuclear receptor binding SET domain protein-3 (NSD3) is a histone lysine methyltransferase and a crucial regulator of carcinogenesis in several cancers. We aimed to investigate the prognostic value and potential function of NSD3 in 33 types of human cancer. Methods: The data were obtained from The Cancer Genome Atlas. Kaplan-Meier analysis, CIBERSORT, gene set enrichment analysis, and gene set variation analysis were performed. The expression of NSD3 was measured using quantitative real-time polymerase chain reaction and western blot. Results: The expression of NSD3 was altered in pan-cancer samples. Patients with higher levels of NDS3 generally had shorter overall survival… More >

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    ARTICLE

    Comprehensive bioinformatics analysis of CYB561 expression in breast cancer: Link between prognosis and immune infiltration

    XI YANG1,5,#, HUIXIAN WU2,3,#, CHAO XIONG4,#, BO ZHAO1, MEILIAN LIU1, JIE QIN6, MEI DENG1,2,3,*
    BIOCELL, Vol.47, No.5, pp. 1021-1037, 2023, DOI:10.32604/biocell.2023.027103
    Abstract Background: Cytochrome b561 (CYB561) plays a critical role in neuroendocrine function, cardiovascular regulation, and tumor growth; however, the prognostic value of CYB561 in patients with breast cancer and the relationship between CYB561 expression and immune infiltration in breast cancer remain unclear. Methods: The mRNA expression and clinical data of patients with breast cancer were obtained from The Cancer Genome Atlas database. Functional enrichment analysis was used to explore underlying biological functions associated with CYB561. The methylation status of CYB561 was analyzed using the MethSurv database. The enrichment score of immune cell infiltration for CYB561 in breast cancer was calculated using… More >

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    ARTICLE

    The extracellular secretion of miR-1825 wrapped by exosomes increases CLEC5A expression: A potential oncogenic mechanism in ovarian cancer

    QIAOLING WU1,2, ZHAOLEI CUI3, HONGMEI XIA1, SHAN JIANG1, JING BAI4, ZHUO SHAO4, YANG SUN1,*
    BIOCELL, Vol.47, No.5, pp. 1039-1050, 2023, DOI:10.32604/biocell.2023.027494
    Abstract Background: Ovarian cancer (OC) is a leading cause of gynecological cancer-linked deaths worldwide. Exosomal miR-1825 and its target gene C-type lectin domain family 5 member A (CLEC5A) are associated with tumorigenesis in cancers that was further probed. Methods: Exosomal miR-1825 expression in exosomes and its impact on overall survival (OS) prediction were determined using Gene Expression Omnibus (GEO) and The Cancer Genome Atlas (TCGA) data. Target genes of miR-1825 were searched in five prediction databases and prognostically significant differentially expressed genes were identified. Gene Ontology (GO) and Kyoto Encyclopedia of Genes and Genomes (KEGG) enrichment analyses were carried out. The… More >

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    ARTICLE

    Raloxifene-loaded and aptamer-bonded exosomes induce autophagic and apoptotic death in HeLa cells by enhancing the lysosomotropic effect

    OMER ERDOGAN1, GULEN MELIKE DEMIRBOLAT2, OZGE CEVIK1,*
    BIOCELL, Vol.47, No.5, pp. 1051-1063, 2023, DOI:10.32604/biocell.2023.028129
    Abstract Background: Raloxifene, a selective estrogen receptor modulator, is also known to be a lysosomotropic agent. The bioavailability of raloxifene is around 2% due to extensive hepatic transport. Exosomes are nanosized vesicles that are naturally released from cells. Method: In this study, exosomes released from HeLa cervical cancer cells were loaded with raloxifene to increase its bioavailability, and an aptamer was attached to the exosome membrane for targeting only HeLa cells. Characterization of exosomes isolated from HeLa cells was performed by transmission electron microscopy, zeta sizer, and western blotting. In addition, the cytotoxic, apoptotic, autophagic, and lysosomotropic effects of the prepared… More >

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    ARTICLE

    Analysis of the personalized treatment and the relevant prognostic factors in children with medulloblastoma

    LIHUA CHEN1,2,#, HONGTIAN ZHANG1,2,#, YONG XIA1,2, KAI SUN1, WENJIN CHEN1, RUXIANG XU1,2,*
    BIOCELL, Vol.47, No.5, pp. 1065-1073, 2023, DOI:10.32604/biocell.2023.025924
    Abstract Purpose: The present study summarized cases of children (n = 32) with medulloblastoma (MB) who were treated using stratified therapy based on risk grading and also discussed the factors affecting prognosis. Methods: According to the risk stratification criteria, the cases were divided into the following four risk groups: low, standard, high, and very high. The 5-year overall survival (OS) and progression-free survival (PFS) rates were summarized. Further, the effects on the prognosis of tumor size, tumor stage, degree of resection, treatment mode, metastatic recurrence, molecular typing, and risk stratification were analyzed. Results: In the present study, following surgery, 3 cases… More >

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    ARTICLE

    Dihydroartemisinin enhances cell apoptosis in diffuse large B cell lymphoma by inhibiting the STAT3 activity

    ZHENG CAO1,#, CHUNXIAO ZHOU1,#, ZHIMIN WU1, CHUNYAN WU1, WEN ZHANG1, SHILV CHEN1, XINDONG ZHAO1, SHAOLING WU2,*
    BIOCELL, Vol.47, No.5, pp. 1075-1083, 2023, DOI:10.32604/biocell.2023.027027
    Abstract Background: Dihydroartemisinin (DHA) is reported to be a potential anticancer agent, and the mechanisms underlying the effects of DHA on diffuse large B cell lymphoma however are still obscure. This study aimed to assess the antitumor effect of DHA on diffuse large B cell lymphoma cells and to determine the potential underlying mechanisms of DHA-induced cell apoptosis. Methods: Here, the Cell Counting Kit 8 assay was conducted to study cell proliferation. We performed Annexin V-FITC/propidium iodide staining, real-time polymerase chain reaction, and western blot analysis to analyze cell apoptosis and potential molecular mechanisms. Results: The results showed that DHA substantially… More >

  • Open AccessOpen Access

    ARTICLE

    Immunoregulatory effects of human amniotic mesenchymal stem cells and their exosomes on human peripheral blood mononuclear cells

    XIN TIAN, XIANGLING HE*, SHUQIN QIAN, RUNYING ZOU, KEKE CHEN, CHENGGUANG ZHU, ZEXI YIN
    BIOCELL, Vol.47, No.5, pp. 1085-1093, 2023, DOI:10.32604/biocell.2023.027090
    Abstract Background: The immunomodulatory effects of mesenchymal stem cells (MSCs) and their exosomes have been receiving increasing attention. This study investigated the immunoregulatory effects of human amniotic mesenchymal stem cells (hAMSCs) and their exosomes on phytohemagglutinin (PHA)-induced peripheral blood mononuclear cells (PBMCs). Methods: The hAMSCs used in the experiment were identified by light microscopy and flow cytometry, and the differentiation ability of the cells was determined by Oil Red O and Alizarin Red staining. The expressions of transforming growth factor (TGF)-β, indoleamine 2,3-dioxygenase (IDO), cyclooxygenase-2 (COX-2), hepatocyte growth factor (HGF), and interleukin (IL)-6 were detected by quantitative real-time polymerase chain reaction… More >

  • Open AccessOpen Access

    ARTICLE

    Effects of docosahexaenoic acid or arachidonic acid supplementation on the behavior of cardiomyocytes derived from human pluripotent stem cells

    MIZUNA YANO1, KOTA HIROI1, TETSUYA YUASA1, KENJI INOUE1, OSAMU YAMAMOTO1, TAKAO NAKAMURA2, DAISUKE SATO1, ZHONGGANG FENG1,*
    BIOCELL, Vol.47, No.5, pp. 1095-1106, 2023, DOI:10.32604/biocell.2023.028186
    Abstract Background: Human heart changes its energetic substrates from lactate and glucose to fatty acids during the neonatal period. Noticing the lack of fatty acids in media for the culture of cardiomyocytes derived from human pluripotent stem cells (hiPS-CM), researchers have supplemented mixtures of fatty acids to hiPS-CM and reported the enhancement in the maturation of hiPS-CM. In our previous studies, we separately supplemented two polyunsaturated fatty acids (PUFAs), docosahexaenoic acid (DHA) or arachidonic acid (AA), to rat fetal cardiomyocytes and found that the supplementations upregulated the expressions of mRNAs for cardiomyocyte differentiation, fatty acid metabolism, and cellular adhesion. The enhancement… More >

  • Open AccessOpen Access

    ARTICLE

    Tanshinone IIA protects intestinal epithelial cells from ferroptosis through the upregulation of GPX4 and SLC7A11

    HAN WANG1,2,#, YANG SUN1,2,#, XIAOXU ZHANG2,3, XIAOYING WANG4, YUJUN XIA1,*, LISHENG WANG1,2,*
    BIOCELL, Vol.47, No.5, pp. 1107-1115, 2023, DOI:10.32604/biocell.2023.027131
    Abstract Background: Inflammatory bowel disease (IBD) is a chronic inflammatory disease of the gastrointestinal tract. The destruction of the intestinal epithelial barrier is one of the major pathological processes in IBD pathology. Growing evidence indicated that epithelial cell ferroptosis is linked to IBD and is considered a target process. Methods: RAS-selective lethal 3 (RSL3) was used to induce ferroptosis in intestinal epithelial cell line No. 6 (IEC-6) cells, and cell ferroptosis and the effects of tanshinone IIA (Tan IIA) were determined by cell counting kit-8 (CCK-8), reactive oxygen species (ROS) staining, Giemsa staining and transmission electron microscope (TEM). The cell viability… More >

  • Open AccessOpen Access

    ARTICLE

    LINC00609 inhibits A549 cells progression through the regulation of miR-128-3p/RND3 axis

    XIANGCHAO DING1,#, YANG ZHAO2,#, XINGHUA ZHANG1, HUIQING LIN1,*
    BIOCELL, Vol.47, No.5, pp. 1117-1126, 2023, DOI:10.32604/biocell.2023.026715
    Abstract Background: Long-chain non-coding RNA (lncRNA) LINC00609 is a potential tumor suppressor, but the mechanism of action in non-small cell lung cancer (NSCLC) is yet to be understood.Objectives: The effects of LINC00609 on A549 cell proliferation, apoptosis, and cell cycle arrest were investigated. Methods: The LINC00609 levels in NSCLC and normal tissues were analyzed by bioinformatics. Expressions of LINC00609, miR-128-3p, and Rho family GTPase 3 (RND3) in NSCLC cells (A549) were determined by qRT-PCR. Bioinformatics analysis predicted target genes and dual-luciferase reporter assays to ensure that LINC00609 targeted miR-128-3p and miR-128-3p targeted RND3. The proliferation of cells was determined using EDU… More >

  • Open AccessOpen Access

    ARTICLE

    Are the two polymorphic sites of anti-Marek’s disease in White Leghorn chickens also suitable for Partridge Shank chickens?

    WENQING LI1, WANLI LI2,3, BEI WANG1, LIN ZHANG1, SHENGLI LI3, CHENWAN LI1, PINHUI WU1, GUOQING YANG1, GUOZHI ZHANG1,*
    BIOCELL, Vol.47, No.5, pp. 1127-1134, 2023, DOI:10.32604/biocell.2023.027863
    (This article belongs to this Special Issue: )
    Abstract Background: The selection of Marek’s disease (MD)-resistant breeds in Partridge Shank chicken, a popular local chicken breed in Henan Province of China, has practical value. We hypothesized that the two polymorphic sites (rs14527240 located in SMOC1 and GGaluGA156129 located in PTPN3) related to MD resistance in White Leghorn chickens are also applicable to Partridge Shank chickens. Methods: In this experiment, we screened 10 live hens and 2 live roosters with the double GG genotype by genotyping the two sites from 6500 Partridge Shank chickens. Nineteen one-day-old chicks with the double GG genotype were obtained by artificial insemination. Seventy-two one-day-old chickens… More >

  • Open AccessOpen Access

    ARTICLE

    Calcitriol induces post-thawed bovine sperm capacitation

    ANA CECILIA LIAUDAT, FRANCISCA EBEL, BIANCA ANA OPIZZO, MARINA AURORA GONZALEZ, DAMIÁN BLOIS, PABLO BOSCH#, NANCY RODRIGUEZ#,*
    BIOCELL, Vol.47, No.5, pp. 1135-1143, 2023, DOI:10.32604/biocell.2023.027628
    Abstract Background: Capacitation is a set of physiological changes sperms undergo to acquire fertilizing capacity. In vivo, this process is directly associated with high calcium levels in sperm cytoplasm. Calcitriol, the vitamin D hypercalcemic metabolite, is related to human sperm motility, capacitation, and acrosome reaction. This work aimed to study the effect of calcitriol on bull sperm quality parameters and capacitation. Methods: One million freeze-thawed spermatozoa were obtained from different bulls and treated with 20 nM of calcitriol for 30 min. Untreated cells (negative control) and treated ones with calcitriol or heparin (100 µg/mL, positive capacitation control) were evaluated for motility,… More >

  • Open AccessOpen Access

    ARTICLE

    A Brachypodium distachyon calcineurin B-like protein-interacting protein kinase, BdCIPK26, enhances plant adaption to drought and high salinity stress

    QINGCHEN LUO1,#,*, JIALU FENG2,#, XIUQI DENG1
    BIOCELL, Vol.47, No.5, pp. 1145-1158, 2023, DOI:10.32604/biocell.2023.027847
    Abstract As sessile organisms, plants possess a complex system to cope with environmental changes. Ca2+ functions as a vital second messenger in the stress signaling of plants, and the CBL-interacting protein kinases (CIPKs) serve as essential elements in the plant Ca2+ signaling pathway. In this study, calcineurin B-like protein-interacting protein kinase 26 (BdCIPK26) from Brachypodium distachyon was characterized. Overexpression of BdCIPK26 enhanced tolerance to drought and salt stress of transgenic plants. Further investigations revealed that BdCIPK26 participated in abscisic acid (ABA) signaling, conferred hypersensitivity to exogenous ABA in transgenic plants, and promoted endogenous ABA biosynthesis. Moreover, BdCIPK26 was found to maintain… More >

  • Open AccessOpen Access

    REVIEW

    Phytochemistry and ethnomedicinal qualities of metabolites from Phyllanthus emblica L.: A review

    VIJAY KUMAR1,#, PRAVEEN C. RAMAMURTHY2,#, SIMRANJEET SINGH2,#, DALJEET SINGH DHANJAL3, PARUL PARIHAR4, DEEPIKA BHATIA5, RAM PRASAD6,*, JOGINDER SINGH7,*
    BIOCELL, Vol.47, No.5, pp. 1159-1176, 2023, DOI:10.32604/biocell.2023.022065
    Abstract Phyllanthus emblica or Indian gooseberry is an integrated part of Ayurvedic and Traditional Chinese Medicines. For several decades, the well-known ancient herb has been extensively utilized in traditional medicine to cure diseases like fever, diabetes, constipation, jaundice, ulcers, biliousness, anemia, anorexia, and dyspepsia. In the traditional system, Indian gooseberry has various ethnomedicinal applications. In the Ayurvedic system, different methods of administration (anupan) have shown different ethnomedicinal properties of Indian gooseberry. Seventy well-known chemical components in Indian gooseberry have been identified through phytochemical evaluation, among which the flavonoids and phenols are most prominent. From the toxicity perspective, it is considered a… More >

  • Open AccessOpen Access

    REVIEW

    Fe-dependent cellular alterations of oxidative balance in aquatic organisms. Could be ferroptosis involved?

    PAULA MARIELA GONZÁLEZ1,2, JOAQUIN CABRERA1,2, SUSANA PUNTARULO1,2,*
    BIOCELL, Vol.47, No.5, pp. 1177-1189, 2023, DOI:10.32604/biocell.2023.027107
    Abstract

    The purpose of this review is to briefly summarize the central role of iron (Fe) in terms of cellular alterations of the oxidative/protective balance with special emphasis on its possible involvement in ferroptosis-dependent disruption in aquatic organisms. In ferroptotic cells or tissues, the intracellular Fe level increases; meanwhile the treatment with Fe chelators limits ferroptosis. Eukaryotic algae can assimilate Fe from the environment through several mechanisms, and aquatic animals incorporate dissolved Fe and Fe bound to both inorganic particles and organic matter. The central role of lipid peroxidation mediating ferroptosis was demonstrated in some algae where both low and high… More >

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