Open Access
REVIEW
María P BENAVIDES1, Susana M GALLEGO1, Facundo RAMOS ARTUSO2, Mariana CHECOVICH3, Andrea GALATRO*
BIOCELL, Vol.40, No.1, pp. 1-6, 2016, DOI:10.32604/biocell.2016.40.001
Abstract Plants are exposed to environmental stress, in natural and agricultural conditions.Nitric oxide
(NO), a small gaseous molecule which plays important roles in plants, has been involved in many physiological
processes, and emerged as an important endogenous signaling molecule in the adaptation of plants to biotic and
abiotic stress. NO is produced from a variety of enzymatic and non enzymatic sources, which are not yet fully
understood. Also, NO and reactive nitrogen species (RNS) can produce posttranslational modifications affecting
protein function. Nitrate reductase, a key enzyme in the nitrogen metabolism, is a proposed source of NO in
plants which could be… More >
Open Access
ARTICLE
S.S. Bombicino*, D.E. Iglesias, I.A. Rukavina Mikusic, A. Boveris, L.B. Valdez
BIOCELL, Vol.40, No.1, pp. 7-10, 2016, DOI:10.32604/biocell.2016.40.007
Abstract Diabetic cardiomyopathy, i.e. the ventricular dysfunction in the absence of hypertension or coronary arterial disease, is a common complication of diabetes mellitus that leads to a heightened risk of heart failure and death among diabetic patients. This contractile dysfunction could be associated to mitochondrial dysfunction, in which mitochondrial biogenesis could emerge as a compensatory mechanism triggered in response to hyperglycemia. It has been proposed that nitric oxide synthase activities with enhanced NO production are involved in this process. Alterations in the contractile response and lusitropic reserve were observed in streptozotocin diabetic rats after β-adrenergic stimuli. Additionally, tissue O2 consumption was… More >
Open Access
ARTICLE
Juanita BUSTAMANTE1,*, Analía CZERNICZYNIEC2, Silvia LORES-ARNAIZ2
BIOCELL, Vol.40, No.1, pp. 11-14, 2016, DOI:10.32604/biocell.2016.40.011
Abstract The suppressive effects of ketamine on intracellular calcium has been reported in a variety of cells
although the mechanisms involved are not well understood. The aim of this work was to evaluate the ketamine
effect on the mitochondrial Ca2+ accumulation and the cellular Ca2+ mobilization using FLUO4-AM and flow
cytometry. The results showed that mitochondria from ketamine injected animals presented a lower ability to
retain calcium at concentrations higher than 20 μM, as compared with controls (saline injected animals). In addition, ketamine showed a significant decreased KCl-induced intracellular calcium concentration. KCl increased
calcium influx through cellular depolarization. According to the… More >
Open Access
ARTICLE
Jimena P. CABILLA, Sonia A. RONCHETTI, Beatriz H. DUVILANSKI*
BIOCELL, Vol.40, No.1, pp. 15-18, 2016, DOI:10.32604/biocell.2016.40.015
Abstract Environmental contamination with some metalloids and heavy metals (M/HM) raises concern due
to well known adverse effects on health. Among these pollutants, chromium VI (Cr VI), cadmium (Cd) and arsenic (As) are frequently present as a result of natural sources or due to industrial activities. They are able to easily
enter the organism and negatively affect many organs and systems. In vivo (exposure to Cr VI, Cd or As through
drinking water) and in vitro experiments (primary pituitary cell cultures) were performed in male Wistar rats
to address their actions on hypothalamus-pituitary axis. All the M/HM accumulated in hypothalamus and… More >
Open Access
ARTICLE
Nidia FERRAROTTI1, Rosario MUSACCO-SEBIO2, Christian SAPORITO-MAGRIÑÁ2, Juan Manuel ACOSTA2, Marisa REPETTO2 *
BIOCELL, Vol.40, No.1, pp. 19-22, 2016, DOI:10.32604/biocell.2016.40.019
Abstract The objective of this work was to study the in vivo time course of biochemical processes of oxidative
damage in the brain of Sprague-Dawley rats that received an acute overload of the redox active metals iron (Fe)
and copper (Cu), and the redox inactive cobalt (Co) and nickel (Ni). Oxidative stress indicators (phospholipid and
protein oxidation), glutathione (GSH), antioxidant enzymes and NADPH oxidase activities, and the plasma inflammatory cytokine (IL-6) were measured. The results showed that in brain oxidative mechanisms for both sets of metal
are different, however in both cases are irreversible. The mechanism for Fe and Cu oxidative… More >
Open Access
ARTICLE
Marcelo HERNANDO1, Christian HOUGHTON1, Leda GIANNUZZI2, Bernd KROCK3, Darío ANDRINOLO2, Gabriela MALANGA4,*
BIOCELL, Vol.40, No.1, pp. 23-26, 2016, DOI:10.32604/biocell.2016.40.023
Abstract Cyanobacteria are phototrophic organisms with great ecological and economical importance.
Species of the genus Microcystis are known for their potential ability to synthesize toxins, notably microcystins.
There is a growing interest in the evaluation of oxidative stress in relation to the impact of global climate change
on natural ecosystems in different trophic levels. Several studies have focused on the analysis of organismal
responses to mitigate the damage by controlling the generation of reactive oxygen species. Variations in environmental factors caused by climate change generate a situation of oxidative damage in Microcystis aeruginosa as a
direct or indirect consequence. In this… More >
Open Access
ARTICLE
Darío E. IGLESIAS*, Silvina S. BOMBICINO, Alberto BOVERIS, Laura B. VALDEZ
BIOCELL, Vol.40, No.1, pp. 27-30, 2016, DOI:10.32604/biocell.2016.40.027
Abstract Complex III plays a central role in the mitochondrial respiratory chain transferring electrons from
ubiquinol to cytochrome c and pumping protons to the intermembrane space, contributing to the protonmotive
force. Furthermore, complex III can act as a source of O2
•- in the presence of ubiquinol and antimycin, an
expermiental condition in which the oxidation of the cytochrome b hemes is blocked. The O2
•- dismutation
catalyzed by superoxide dismutase produces H2O2, a known second messenger in redox signalling. Results from
our laboratory have shown that NO, released from GSNO or from SPER -NO or generated by mtNOS, inhibits
electron… More >
Open Access
ARTICLE
Analia G. Karadayian1, Juanita Bustamante2, Silvia Lores-Arnaiz1, *
BIOCELL, Vol.40, No.1, pp. 31-34, 2016, DOI:10.32604/biocell.2016.40.031
Abstract Alcohol hangover (AH) is defined as the temporary state after alcohol binge-like drinking, starting
when EtOH is absent in plasma. Results from our laboratory have shown behavioral impairments and mitochondrial dysfunction in an experimental model of AH in mice. Our model consisted in a single i.p. injection of EtOH
(3.8 g/kg BW) or saline solution in male and female mice, sacrificing the animals 6 hours after injection. Motor and
affective behavior together with mitochondrial function and free radical production were evaluated in brain cortex
and cerebellum during AH. Results showed that hangover animals exhibited a significant reduction in neuromuscular coordination,… More >
Open Access
ARTICLE
Gabriela MALANGA, Paula Mariela GONZÁLEZ, Juan Manuel OSTERA, Susana PUNTARULO*
BIOCELL, Vol.40, No.1, pp. 35-38, 2016, DOI:10.32604/biocell.2016.40.035
Abstract The harmful effects of the reactive species may be due to the increase in their steady state
concentration either by the enhancement of their production rates and/or the decrease of their consumption
rate by antioxidant activity. The ascorbyl radical (A•
) can be considered as a final product of radical oxidative
transformations of ascorbate (AH-). The ratio A•
content/AH- content (A•
/AH-) has been widely used as an
interesting tool to estimate mild to moderate oxidative transformations, providing a quick and simple method
of diagnosis of stress in the hydrophilic cellular medium. The aim of this work was to summarize… More >
Open Access
ARTICLE
Natacha E PILONI, Elizabeth ROBELLO, Julián G BONETTO, Susana PUNTARULO*
BIOCELL, Vol.40, No.1, pp. 39-42, 2016, DOI:10.32604/biocell.2016.40.039
Abstract Fe is essential for human life because it constitutes the required cofactor for proteins of diverse biological
functions. However, the development of oxidative stress by exposure to excessive Fe, share signaling pathways with
other treatments including activation of redox-sensitive factors. This study was focused on the comparison on the
effects of Fe in the brain and other organs in vivo. The oxidative effects triggered by Fe overload strongly depend
not only on the administration protocol, but also on the Fe-compound used, and the studied organ. In both the liver
and the brain, Fe content drastically increased after Fe-dextran administration. However,… More >
Open Access
ARTICLE
M. Rodríguez, B. Buchholz, V. D’Annuzio, M. Donato, G.E. González, M. A. Goyeneche, T. Mazo, V. Pérez, L. Wilensky, R.J. Gelpi*
BIOCELL, Vol.40, No.1, pp. 43-46, 2016, DOI:10.32604/biocell.2016.40.043
Abstract Dystrophin is a membrane-associated protein responsible for structural stability of the sarcolemma in cardiac myocytes and is very sensitive to ischemic damage. The goal of our study was to determine if ischemic preconditioning could prevent dystrophin breakdown through inhibition of matrix metalloproteinase-2 (MMP-2) activity. Isolated rabbit hearts were subjected to global ischemia with or without reperfusion in order to evaluate if dystrophin is preserved by ischemic preconditioning through MMP-2 inhibition. Ischemic preconditioning significantly reduced the infarct size induced by 30 min of ischemia and 180 min of reperfusion. Importantly, it also diminished dystrophin proteolysis and attenuated MMP-2 activity after 30… More >
Open Access
ARTICLE
Virginia VANASCO, Timoteo MARCHINI, Natalia MAGNANI, Tamara VICO, Mariana GARCES, Lourdes CACERES, Alejandro GUAGLIANONE, Pablo EVELSON, Silvia ALVAREZ.*
BIOCELL, Vol.40, No.1, pp. 47-50, 2016, DOI:10.32604/biocell.2016.40.047
Abstract Several authors have addressed the importance of mitochondrial function in inflammatory syndromes, as it may play a role in the genesis of tissue injury. Sepsis and exposition to environmental particles are
examples of inflammatory conditions. Sepsis occurs with an exacerbated inflammatory response that damages
tissue mitochondria and impairs bioenergetic processes. One of the current hypotheses for the molecular mechanisms underlying the complex condition of sepsis is that enhanced NO production and oxidative stress lead to
mitochondrial dysfunction, bioenergetic derangement and organ failure. The mechanism of particulate matter-health effects are believed to involve inflammation and oxidative stress. Components in particles that… More >
Open Access
ARTICLE
Claudia G. REIDES1,2, Romina M. LASAGNI VITAR1,2, Agustina PEVERINI1, Natasha S. JANEZIC1, Ailen G. HVOZDA ARANA1, Sandra M. FERREIRA1,2, Susana F. LLESUY1,2*
BIOCELL, Vol.40, No.1, pp. 51-54, 2016, DOI:10.32604/biocell.2016.40.051
Abstract The purpose of this study was to evaluate the redox status changes of primary visual targets in the rat
brain of a high pressure-induced glaucoma model. The animal model consisted of inducing ocular hypertension by
cauterizing two episcleral veins on the left eye. The markers of oxidative damage and the oxidative balance evaluated
in the brain seven days postoperative were: nitrites concentration, levels of non-enzymatic antioxidants and antioxidant enzymes activity.
The increase in the nitrite content, which could be the result of the enhancement in the production of nitrogen
species, and in the activity of NADPH oxidase in the glaucoma… More >
Open Access
ARTICLE
Tamara ZAOBORNYJ, Darío E. IGLESIAS, Silvina S. BOMBICINO, Alberto BOVERIS, Laura B. VALDEZ*
BIOCELL, Vol.40, No.1, pp. 55-58, 2016, DOI:10.32604/biocell.2016.40.055
Abstract Normal cardiac function is accomplished through a continuous energy supply provided by
mitochondria. Heart mitochondria are the major source of reactive oxygen and nitrogen species: superoxide
anion (O2-) and nitric oxide (NO). NO production by mitochondrial NOS (mtNOS) is modified by metabolic
state and shows an exponential dependence on Δψ. The interaction between mtNOS and complexes I and IV
might be a mechanism involved in the regulation of mitochondrial NO production. NO exerts a high affinity,
reversible and physiological inhibition of cytochrome c oxidase activity. A second effect of NO on the respiratory
chain is accomplished through its interaction with… More >
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