Home / Journals / BIOCELL / Vol.28, No.2, 2004
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  • Open AccessOpen Access

    ARTICLE

    Histomorphological and quantitative immunohistochemical changes in the rat pancreas during aging

    F.L. RICCILLO, M.I. BRACAMONTE, G.M. CÓNSOLE, C.L.A. GÓMEZ DUMM
    BIOCELL, Vol.28, No.2, pp. 127-134, 2004, DOI:10.32604/biocell.2004.28.127
    Abstract Although the endocrine pancreas is the purpose of several deep investigations, morphological data referred to the effect of aging on the gland are not homogeneous. The purpose of the current work was to analyze the changes occurring in the pancreas of aged rats, with especial reference to the islet cell populations. Six young (Y), old (O) and senescent (S) male Sprague-Dawley rats were used. The pancreas tails were processed for light microscopy and studied by means of routine stains as well as by immunohistochemical identification of insulin-, glucagon-, somatostatin-, and pancreatic polypeptide- secreting cells (Dako Envision System, DAB as chromogen).… More >

  • Open AccessOpen Access

    ARTICLE

    Study of the myenteric and submucous plexuses after BAC treatment in the intestine of rats

    NILZA CRISTINA BUTTOW, MIRIAM SANTIN, LUCIANA CONCI MACEDO, ALINE CRISTINA NERES TEIXEIRA, GISELE CAROLINE NOVAKOWSKI, TAÍSE ROBERTA BOLONHEIS ARMELIN, KATHYA ASSMANN
    BIOCELL, Vol.28, No.2, pp. 135-142, 2004, DOI:10.32604/biocell.2004.28.135
    Abstract A morphological and quantitative study in the ileal and colonic myenteric and submucous plexuses of rats after BAC denervation was performed. Four groups were employed: SI – ileum control; CBI – denervated ileum; SC – colon control; and CBC – denervated colon. We used the Myosin-V immunohistochemistry technique to study the myenteric and submucous plexuses. In the submucous plexus of the ileum and colon there was not a significant decrease in the number of neurons/mm2 and of ganglia/mm2 . The denervation of the myenteric plexus in the group CBI was 44.7% and in the group CBC, 68.3%. In the myenteric… More >

  • Open AccessOpen Access

    ARTICLE

    Probiotics enhance the recovery of gut atrophy in experimental malnutrition

    DIANA B. DOCK*, JOSÉ E. AGUILAR-NASCIMENTO**, MARCIA Q. LATORRACA*
    BIOCELL, Vol.28, No.2, pp. 143-150, 2004, DOI:10.32604/biocell.2004.28.143
    Abstract AIM: The aim of this study was to evaluate the effect of probiotics on the recovery of the bowel atrophy induced by malnutrition in rats. METHODS: Twenty-and-six Wistar rats (200-250g) were fed with either a normoproteic (sham group, n=6) or a free-protein diet (n=20) during 12 days. Twelve malnourished rats were randomized to recover during 15 days with either a hydrolyzed diet (control group, n=6) or the same diet enriched with probiotics (Streptococcus thermophilus and Lactobacillus helveticus; probiotic group, n=6). RESULTS: Probiotic group showed similar gain of body, liver and bowel weight than controls. At the jejunum, both the villus… More >

  • Open AccessOpen Access

    ARTICLE

    Fine-structural details of the photoreceptor membranes in the ocellus of the scale-insect parasite Centrodora sp. (Hymenoptera; Aphenelidae): A case of gene transfer between host and parasite?

    MOUYU YANG1, V.BENNO MEYER-ROCHOW1,2
    BIOCELL, Vol.28, No.2, pp. 151-154, 2004, DOI:10.32604/biocell.2004.28.151
    Abstract Only one insect (the scale insect Eriococcus sp.) is known, in which photoreceptive lamellae appear to have replaced the usual arthropod rhabdom microvilli. We are now reporting the presence of photoreceptive membranes, which also appear to resemble lamellae rather than microvilli, but they are in the ocellus of the tiny wasp Centrodora sp., which parasitizes scale insect eggs. The apparently optically homogenous lens of the Centrodora ocellus measures approximately 10 µm in diameter and, thus, operates at the limits of diffraction. We calculated that the lens is capable of focusing a parallel bundle of rays on the retina of the… More >

  • Open AccessOpen Access

    ARTICLE

    Intrahost distribution and trasmission of a new species of cyclopoid copepod endosymbiotic to a freshwater snail, Pomacea canaliculata (Caenogastropoda, Ampullariidae) from Argentina

    C. D. GAMARRA-LUQUES, I. A. VEGA, E. KOCH, A. CASTRO-VAZQUEZ
    BIOCELL, Vol.28, No.2, pp. 155-164, 2004, DOI:10.32604/biocell.2004.28.155
    Abstract A new species of cyclopoid copepod, Ozmana huarpium, is described as a symbiont to Pomacea canaliculata (Lamarck 1822) (Caenogastropoda, Ampullariidae). Rather large numbers (about one hundred copepods per snail) were found, although there was no evidence of harm to the host. To our knowledge, O. haemophila (symbiont to P. maculata), and the currently described species, O. huarpium, are the only copepod species ever recorded as endosymbionts to freshwater invertebrates. While O. haemophila is restricted to the haemocoel of its host, O. huarpium predominate in the penis sheath, the ctenidium and the mantle cavity, figuring in these pallial organs 63-65 %… More >

  • Open AccessOpen Access

    ARTICLE

    Brief Note: Ultrastructure of the Lyonet’s glands in larvae of Diatraea saccharalis Fabricius (Lepidoptera: Pyralidae)

    ELIANE VICTORIANO, ELISA A. GREGÓRIO
    BIOCELL, Vol.28, No.2, pp. 165-169, 2004, DOI:10.32604/biocell.2004.28.165
    Abstract The Lyonet’s gland is found in Lepidoptera larvae, close to the excretory duct of the silk gland. The role played by this gland is still uncertain. This work aims to describe the ultrastructure of the Lyonet’s gland in Diatraea saccharalis larvae, offering suggestions regarding its possible function. The insects were reared under laboratory-controlled conditions. The glands were conventionally prepared for transmission (TEM) and scanning (SEM) electron microscopy. SEM showed that Lyonet’s glands are paired small structures located in the ventral side of the head. They are composed by clustered long cells resembling leaves. Under TEM observations, each cell is surrounded… More >

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