Home / Journals / BIOCELL / Vol.31, No.2, 2007
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  • Open Access

    ARTICLE

    Cloning and analysis of IFRG (interferon responsive gene) in rabbit oocytes and preimplantation embryos

    BING QI1, XINMING ZHENG2, WENYONG LI3, QINGXIN WEI2, QINGXUAN CHEN1
    BIOCELL, Vol.31, No.2, pp. 199-203, 2007, DOI:10.32604/biocell.2007.31.199
    Abstract Although there is more evidence that shows that IFNs (interferons) plays a very important role in the early development of the embryo, the mechanism of IFNs is still unclear. Our study showed that IFRG is expressed from oocytes- through to the preimplantation embryo in rabbits. This finding provides some clues for better understanding the role of IFNs in the development of the embryo. The full length of rabbit IFRG cDNA (Accession No. AJ584672), with a 2794bp encoding 131 amino acid sequence, was cloned IFRG expression can be detected in 8 different tissues: ovary, heart, lung, liver, kidney, spleen, cerebra, and… More >

  • Open Access

    ARTICLE

    Nitric oxide synthase activity in tissues of the blowfly Chrysomya megacephala (Fabricius, 1794)

    A. C. FARALDO1, A. SÁ-NUNES2, L. H. FACCIOLI2, E. A. DEL BEL3, E. LELLO4
    BIOCELL, Vol.31, No.2, pp. 205-211, 2007, DOI:10.32604/biocell.2007.31.205
    Abstract Although insects lack the adaptive immune response of the mammalians, they manifest effective innate immune responses, which include both cellular and humoral components. Cellular responses are mediated by hemocytes, and humoral responses include the activation of proteolytic cascades that initiate many events, including NO production. In mammals, nitric oxide synthases (NOSs) are also present in the endothelium, the brain, the adrenal glands, and the platelets. Studies on the distribution of NO-producing systems in invertebrates have revealed functional similarities between NOS in this group and vertebrates. We attempted to localize NOS activity in tissues of naïve (UIL), yeast-injected (YIL), and saline-injected… More >

  • Open Access

    ARTICLE

    p53-Rb signaling pathway is involved in tubular cell senescence in renal ischemia/reperfusion injury

    LI KAILONG1,2, DU XIAOLAN1, HE YANI2, ZHAO LIN1, YANG JVRONG2, SONG RUIHUA1, CHEN LIN1
    BIOCELL, Vol.31, No.2, pp. 213-223, 2007, DOI:10.32604/biocell.2007.31.213
    Abstract Objective: To investigate the course of tubular cell senescence and expressions of p53, p21, and Rb during the late phase of ischemia/reperfusion (IRI) in the kidney, and assess the effects of the p53-Rb pathway on tubular cell senescence. Methods: Experimental models of unilateral renal IRI were used in p53(+/+) and p53(-/-) mice. Histological changes at the tubular level, progress of cell senescence, and the expression of Rb, p21, and/or p53 proteins in tubular cells were studied at different moments in time after IRI. Results: Chronic tubulointerstitial fibrosis was much more severe and widely distributed in IRI kidneys of p53(+/+) mice… More >

  • Open Access

    ARTICLE

    Ultrastructural analysis and identification of membrane proteins in the free-living amoeba Difflugia corona

    MARCELO SILVA-BRIANO1 , SANDRA LUZ MARTÍNEZ-HERNÁNDEZ1 , ARACELI ADABACHE-ORTÍZ1 , JAVIER VENTURA-JUÁREZ2 , EVA SALINAS3 , J. LUIS QUINTANAR4
    BIOCELL, Vol.31, No.2, pp. 225-228, 2007, DOI:10.32604/biocell.2007.31.225
    Abstract Syntaxin-1 and 25-kDa Synaptosome-associated Protein (SNAP-25) are present in the plasma membrane of several different secretory cell types and are involved in the exocytosis process. In this work, the free-living amoeba Difflugia corona was studied in relation to ultrastructure, structural membrane proteins, and proteins such as Syntaxin-1 and SNAP-25. Our results obtained by scanning electron microscopy in the amoeba without its theca, showed many membrane projections and several pore-like structures. Using immunocytochemistry, we found structural proteins Syntaxin-1 and SNAP-25. More >

  • Open Access

    ARTICLE

    An ultrastructural study of spermiogenesis in two species of Sitophilus (Coleoptera: Curculionidae)

    KHESLLER PATRÍCIA OLÁZIA NAME, GIULIANO PAGY FELIPE DOS REIS, SÔNIA NAIR BÁO
    BIOCELL, Vol.31, No.2, pp. 229-236, 2007, DOI:10.32604/biocell.2007.31.229
    Abstract The spermiogenesis of Sitophilus zeamais and Sitophilus oryzae, the maize and the rice weevil, respectively, was studied by light microscopy and scanning and transmission electron microscopy. Sitophilus spp. is the most widespread and destructive primary pest of stored cereals in the world. The spermiogenesis occurs within cysts. There are approximately 256 germ line cells per cyst. Inside each cysts, all the spermatids are in the same stage of maturation. The ultrastructure of the spermatozoa of S. zeamais and S. oryzae is similar to that described for other beetles. The head is formed by a three-layered acrosome with the perforatorium, the… More >

  • Open Access

    ARTICLE

    Cell-cell communication between mouse mammary epithelial cells and 3T3-L1 preadipocytes: Effect on triglyceride accumulation and cell proliferation

    VANINA L. JULIANELLI1 , LILIANA N. GUERRA*1, JUAN C. CALVO*1,2
    BIOCELL, Vol.31, No.2, pp. 237-245, 2007, DOI:10.32604/biocell.2007.31.237
    Abstract Interaction between parenchyma and stroma is essential for organogenesis, morphogenesis, and differentiation. Mammary gland has being the chosen model for developmental biologist because the most striking changes in morphology and function take place after birth. We have demonstrated a regulation of triglyceride accumulation by protein factors synthesized by normal mouse mammary gland epithelial cells (NMMG), acting on a cell line, 3T3-L1, long used as a model for adipogenesis. In this paper, we demonstrate that this inhibitory effect seems to be shared by other cells of epithelial origin but not by other cell types. We found a regulation of cell proliferation… More >

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