Vol.46, No.3, 2022-Table of Contents

On the Cover

Annona montana-mediated inhibitory effect on both human and murine adipogenesis was shown to be exerted via a significant decrease in the accumulation of lipid content by both a dramatic reduction of size and number of lipid droplets. Annona montana leaves’ extract strongly attenuated the expression of HMGB2, which correlate with the decrease of the expression of CEBPα, and PPARγ via the modulation of the Akt signaling pathway. Thus, Annona montana leaves’ extract blocks adipogenesis of fibroblasts by selectively altering expression of several adipogenic factors.
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  • Anti-lipid droplets accumulation effect of Annona montana (mountain soursop) leaves extract on differentiation of preadipocytes
  • Abstract The Annona genus is a member of Annonaceae, one of the largest families of plants across tropical and sub-tropical regions. This family has been used in several ethnomedicinal practices to treat a multitude of human diseases. However, the molecular mechanism underlying its effect on the lipid droplet formation and on the expression of adipogenic markers of this plant remain to be investigated. In this study, we examined whether the extracts from the aerial part of Annona montana affect in vitro differentiation of preadipocytes. For our investigations, both mouse embryo fibroblast 3T3-L1 and normal human primary subcutaneous preadipocytes were incubated with… More
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  • The effect of exosomes in transferring TET signaling alterations
  • Abstract Ten eleven translocation (TET) enzymes are composed of three representatives: TET1/2/3 which are involved in the hydroxymethylation of methylated cytosines. Because of the wide array of processes that are governed by these epigenetic marks, there have been a wide range of clinical effects associated with TET alterations. Even though many research groups have focused on analyzing the effect of TET alterations within certain cells, few have taken into consideration the effect of TET in the context of intercellular communication. One important entity through which intercellular communication occurs is represented by exosomes. Thus, in the current viewpoint we discussed the direct… More
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  • Mesenchymal stem cells: As a multi-target cell therapy for clearing β-amyloid deposition in Alzheimer’s disease
  • Abstract Extracellular β-amyloid (Aβ) plaques and neurofibrillary tangles (NFTs) are the pathological hallmarks of Alzheimer’s disease (AD). Studies have shown that aggregates of extracellular Aβ can induce neuroinflammation mediated neurotoxic signaling through microglial activation and release of pro-inflammatory factors. Thus, modulation of Aβ might be a potential therapeutic strategy for modifying disease progression. Recently, a large number of reports have confirmed the beneficial effects of mesenchymal stem cells (MSCs) on AD. It is believed to reduce neuroinflammation, reduce Aβ amyloid deposits and NFTs, increase acetylcholine levels, promote neurogenesis, reduce neuronal damage, and improve working memory and cognition. In this review, we… More
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  • Identification of a three-gene signature in the triple-negative breast cancer
  • Abstract This work aimed to improve current prognostic signatures based on clinical stages in identifying high-risk patients of triple-negative breast cancer (TNBC), to allow patients with a high-risk score for specific treatment decisions. In this study, 396 TNBC samples from TCGA and GEO databases were included in genome-wide transcriptome analysis. The relationship between normalized gene expression values and survival data of patients was determined by Cox proportional hazards models in each dataset. The overlapped genes among all datasets were considered as a potential prognostic signature. The risk score was constructed based on individual genes and validated with three separate data sets… More
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  • Fatty acids in microalgae and cyanobacteria in a changing world: Contrasting temperate and cold environments
  • Abstract Under the present changing climate conditions and the observed temperature increase, it is of high importance to understand its effects on aquatic microbial life, and organisms’ adaptations at the biochemical level. To adjust to temperature or salinity stress and avoid cell damage, organisms alter their degree of fatty acids (FAs) saturation. Thus, temperature is expected to have strong effects on both the quantity and quality of FAs in aquatic microorganisms. Here we review some recent findings about FAs sensitivity to climate change in contrasting environments. Overall, heat waves may induce changes in the relative abundance of polyunsaturated FAs (PUFA). However,… More
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  • Mechanism of tobacco osmotin gene in plant responses to biotic and abiotic stress tolerance: A brief history
  • Abstract Plants are recurrently exposed to myriads of biotic and abiotic stresses leading to several biochemical and physiological variations that cause severe impacts on plant growth and survival. To overcome these challenges, plants activate two primary defense mechanisms, such as structural response (cell wall strengthening and waxy epidermal cuticle development) and metabolic changes, including the synthesis of anti-microbial compounds and proteins, especially the pathogenesis-related (PR) proteins. PR proteins are members of a super large family of defense proteins that exhibit antimicrobial activities. Their over-expression in plants provides tolerance to many abiotic and biotic stresses. PR proteins have been classified into 17… More
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  • Periodontal research contributions to basic sciences: From cell communication and host-parasite interactions to inflammation and bone biology
  • Abstract The periodontium comprises all structures surrounding the teeth, including gingiva, root cementum, periodontal ligament and alveolar bone. Those tissues aim to protect and support the teeth and are challenged by a residing microbiota that leads to subclinical inflammation even in physiological conditions. Periodontitis, a prevalent multicausal inflammatory and destructive disease, develops as a result from complex host-parasite interactions. This unique physiologic and pathologic scenario enables the development of research methods which allows conclusions beyond the simple understanding of periodontal homeostasis. The aim of this viewpoint was to explore potential contributions of periodontal research to a wide array of basic science… More
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  • Cell extrusion in development and cancer, what MARCKS the difference for epithelial integrity?
  • Abstract Cell extrusion is an active mechanism to eliminate non-viable or supernumerary cells in healthy epithelia. It also plays a role in carcinogenesis, both in tumor growth (apical extrusion) and metastasis (basal extrusion). Embryonic tissues like the neuroepithelium, on the other hand, present rates of proliferation comparable to that of carcinomas, without the occurrence of cell extrusion. However, the downregulation or phosphorylation of actin-modulating proteins like MARCKS, causes extensive neuroepithelial apical cell extrusion. As changes in MARCKS proteins phosphorylation and expression have also been correlated to carcinogenesis, we propose here an integrated model for their functions in epithelial integrity. More
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  • Associative learning in plants: light quality history may matter
  • Abstract The possibility of associative learning in plants is a topic of ongoing controversy. In one published study, growing pea plants were reported to associate two stimuli (airflow and light) and thereafter use one (airflow) as an indicator for the other (light), similar to dogs in Pavlov’s famous experiments. However, this observation could not be independently repeated. Here we examine a possible reason for the failure of a published reproduction attempt, which used substantially different light quality during plant cultivation prior to experimental treatments than in the original study. This could have resulted in dramatically different growth characteristics. While the relevance… More
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  • Impact of nanomaterials on plants: What other implications do they have?
  • Abstract The use of nanomaterials has spread and has been applied in different industries, including agriculture. Here, the possibilities presented by NMs are very varied, from the biostimulation of favorable responses, or the control of pests and diseases, to the monitoring of characteristics of interest with the use of nanosensors. Particularly the biostimulation of agricultural crops with the use of nanomaterials is very relevant, since from this process stress tolerance, higher content of biocompounds, etc., can be induced. Although the positive impacts on crops are clear, there is not enough information to determine the long-term impacts, both on ecosystems and on… More
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  • Single-cell RNA sequencing reveals classical monocytes are the major precursors of rat osteoclasts
  • Abstract To dissect which subset of bone marrow monocyte is the major precursor of osteoclast, 3-month-old rat bone marrow was obtained for single-cell RNA sequencing. A total of 6091 cells were acquired for detailed analysis, with a median number of 1206 genes detected per cell and 17,959 genes detected in total. A total of 19 cell clusters were recognized, with the main lineages identified as B cells, Granulocytes, Monocytes, T cells, Erythrocytes and Macrophages. Monocytes were further divided into classical monocytes and non-classical monocytes. Compared with non-classical monocytes, classical monocytes highly expressed osteoclast differentiation related genes Mitf, Spi1, Fos and Csf1r.… More
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  • Upregulation of Zip14 correlates with induction of endoplasmic reticulum stress (ERS) in hypertrophied hearts of Dahl saltsensitive rats
  • Abstract Zinc is a trace element involved in maintaining cellular structure and function. Although zinc is associated with left ventricular hypertrophy (LVH), there have been few reports on this association. This study aimed to evaluate the correlation between Zip14 and expression of endoplasmic reticulum stress (ERS) associated molecules in hypertrophied hearts of rats. Dahl salt-sensitive rats were fed a high salt diet to establish a left ventricular hypertrophy (LVH) rat model. RT-PCR was used to determine Zip14, activating transcription factor (ATF4), ATF6, x-box-binding protein 1 (xBP1), C/EBP homologous protein (CHOP), immunoglobulin-binding protein (BiP) mRNA expression. Western blotting was used to evaluate… More
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  • Curcumin gum Arabic nanoparticles demonstrate potent antioxidant and cytotoxic properties in human cancer cells
  • Abstract The main purpose of the study was to enhance the stability and therapeutic effects of Curcumin (Cur) through nanoformulation with gum Arabic (GA) as a coating agent through an efficient synthetic approach. The antioxidant properties of the developed nanoparticles (Cur/GANPs) were assessed through several in vitro assays, such as β-carotene bleaching activity, DPPH, and nitric oxide scavenging activities in addition to evaluating its inhibitory activity on angiotensin-converting enzyme (ACE). The cytotoxicity of Cur/GANPs was evaluated in vitro using different types of human cancer cells including breast cancer (MCF7, MDA-MB231), liver cancer (HepG2), and colon cancer (HT29) cells. The prepared particles… More
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  • KIAA1199 induces advanced biological behavior and development of ovarian cancer through activation of the IL-6/STAT3 pathway
  • Abstract Recently, abnormal expression of KIAA1199 has been detected in Epithelial Ovarian Cancer (EOC). However, the underlined anti-ovarian cancer mechanism of KIAA1199 remains to be enlightened. In our study, we performed to elucidate the effects of KIAA1199 on the advanced biological behavior of EOC cells through activation of the IL-6/STAT3 pathway. Confirmed by immunohistochemistry, KIAA1199 was highly expressed in ovarian borderline and malignant epithelial tumors. A retrospective analysis found that EOC patients with low expression of KIAA1199 had a significantly higher 5-year survival rate than those with high expression. Mechanistically, IL-6 was used to stimulate EOC cells, and the expression of… More
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  • Hsa_circ_0002137 stabled by LIN28B promotes osteosarcoma cell growth through the hsa-miR-1246/BCL2 axis
  • Abstract Circular RNAs (circRNAs) are a novel class of non-coding RNA that have recently shown to have huge capabilities in the regulation of gene expression at the posttranscriptional level. Growing evidence has indicated that circRNAs could serve as competing endogenous RNAs (ceRNAs) to sponge microRNAs (miRNAs) and suppress functions of targeted miRNAs. Osteosarcoma (OS) is the most common malignant primary bone cancer. Hsa_circ_0002137 is upregulated in OS. However, the role of hsa_circ_0002137 in OS remains unclear. Using miRNA pull-down assay, we showed that cir_0002137 sponged hsa-miR-1246, and BCL2 apoptosis regulator (BCL2) mRNA was a potential target of hsa-miR-1246 in human osteosarcoma… More
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  • A549/DDP derived exosomes can affect cisplatin chemosensitivity via transporting CXCR4 to A549 cells
  • Abstract The resistance of cancer cells to the anti-cancer drugs is the most important reason that affecting the efficacy of the non-small cell lung cancer (NSCLC) chemotherapy; thus, to explore the underlying mechanism of drug resistance of NSCLC medications is urgently needed for improving the therapeutic efficacy of current anti-NSCLC chemotherapies. The aim of the present study is to explore the roles of exosomes in the chemosensitivity of A549 cells and the related mechanism. A549 cells and cisplatin resistant cell line A549/DDP derived exosomes were isolated, and the expressions of CXCR4 were compared. Then, after cisplatin treatment, A549 cells were treated… More
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  • HAND2-AS1, PRKAA2 and VLDLR predict the risk of peritoneal metastasis in gastric cancer of different Lauren types based on STEPP analysis
  • Abstract The peritoneum is the most common site of recurrence of gastric cancer (GC). Early occult peritoneal metastasis is difficult to detect by imaging examination. Stratifying the risk of peritoneal metastasis in patients with different Lauren subtypes is of great clinical value. We performed a univariate Cox regression to identify those genes with prognostic value of overall survival (OS) and peritoneal-specified disease-free survival (psDFS) from the Gene Expression Omnibus database. The candidate genes were screened by the Subpopulation Treatment Effect Pattern Plot (STEPP) method. Propensity score matching (PSM) analysis was used to reduce the interference of confounders on the results. Based… More
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  • Calcium supplementation in colorectal cancer prevention: A systematic meta-analysis of adverse events
  • Abstract Despite the multiple systematic reviews and meta-analyses accumulating evidence on the preventive effect of calcium supplementation for colorectal cancer, most of the associated adverse effects are not systematically analyzed. The aim of the study is evaluating adverse events associated with calcium supplementation for colorectal cancer prevention through a systematic meta-analysis. We searched Medline, PubMed Central, EMBASE (Excerpta Medica database), Scopus, Cochrane Central Register of Controlled Trials, and Web of Science published in English from database inception up to 31 July 2019. In the current systematic meta-analysis, we included human studies (including cohort studies, clinical trials, case-control studies) on supplementation of… More
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  • Uncoupling tumor necrosis factor-α and interleukin-10 at tumor immune microenvironment of breast cancer through miR-17-5p/MALAT-1/H19 circuit
  • Abstract Triple Negative Breast Cancer (TNBC) immunotherapy has recently shown promising approach. However, some TNBC patients presented with resistance. One of the reasons was attributed to the excessive release of cytokines at the tumor microenvironment (TME) such as Tumor necrosis factor alpha (TNF-α) and Interleukin-10 (IL-10). Fine regulation of these cytokines’ levels via non-coding RNAs (ncRNAs) might alleviate the immune quiescent nature of TME at TNBC tumors. However, the extrapolation of ncRNAs as therapeutic tools is highly challenging. Therefore, disentanglement the nature for the isolation of natural compounds that could modulate the ncRNAs and their respective targets is an applicable translational… More
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  • Oxidative effects of glyphosate on the lipophobic intracellular environment in the microalgae Chlorella vulgaris
  • Abstract

    The studied hypothesis is that the herbicide glyphosate (GLY) can affect the oxidative balance in the hydrophobic intracellular medium in non-target Chlorella vulgaris cells. Analytical GLY and RoundUp (RUP) supplementation, affected the growth profile. A significant 42% decrease in the cellular biomass in stationary (St) phase was observed in cultures supplemented with either 5 µM of GLY or RUP, as compared to control cultures. The treatment with 0.3 µM of GLY generated non-significant effects on the oxidation rate of 2’, 7’ dichlorofluorescein diacetate (DCFH-DA), neither in exponential (Exp) nor in St phase of development, as compared to control cultures. However,… More

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  • The chloroplast genome comparative characteristic of artificial breeding tree, a case about Broussonetia kazinoki × Broussonetia papyrifera
  • Abstract Broussonetia kazinoki × Broussonetia papyrifera (ZJGS) is a hybrid species in Moraceae family, which has a very complicated hybrid origin. The excellent characteristics of fast growth, strong soil and water conservation ability, high leaf protein content and stem fiber content in ZJGS make it both ecological benefits in the mining area and economically valuable. This study aims to further understand ZJGS and other Moraceae taxa through the ZJGS chloroplast (cp) genome structure and the comparison with 12 closely related Moraceae species. Among the 13 Moraceae species, the cp genome length of seven Broussonetia species (ranges from 160,239 bp to 162,594… More
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  • A global study of transcriptome dynamics in the venom gland of Solenopsis invicta Buren during laboratory rearing
  • Abstract Venom plays several important roles in the life of the fire ant, Solenopsis invicta Buren. Laboratory rearing significantly affected the toxin component of S. invicta. However, the molecular mechanism of the change of venomous components when kept in the laboratory is unclear. In this study, RNA sequencing technique (RNA-Seq) was performed to explore differentially expressed genes in the venom gland of S. invicta at 0, 10, and 60 days after laboratory rearing. The RNA-Seq results showed that the expression of a large number of genes changed. The DEGs were involved in multiple pathways, including proteolysis, serine-type endopeptidase, and allergen. Furthermore,… More
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  • Mechanical and histological properties of an electrospun scaffold with a modified surface by plasma polymerization implanted in an in vivo model
  • Abstract This article presents the construction of scaffolds composed of polylactic acid (PLA) with different concentrations of hydroxyapatite (HA) by electrospinning, which were superficially modified with polypyrrole (PPy/I) by plasma polymerization. A preliminary study was conducted of the biological and mechanical behavior of the scaffolds when they were implanted in the back of rabbits for 30 days; bone cells differentiated from mesenchymal stem cells (MSCs) were used. The bone cell and scaffold structures were characterized by histological, immunohistochemical, and mechanical stress tests. Hematoxylin–eosin staining showed good tissue conformation. The immunohistochemical tests highlighted the presence of the main bone tissue proteins, such… More
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  • INTS-MFS: A novel method to predict microRNA-disease associations by integrating network topology similarity and microRNA function similarity
  • Abstract Identifying associations between microRNAs (miRNAs) and diseases is very important to understand the occurrence and development of human diseases. However, these existing methods suffer from the following limitation: first, some disease-related miRNAs are obtained from the miRNA functional similarity networks consisting of heterogeneous data sources, i.e., disease similarity, protein interaction network, gene expression. Second, little approaches infer disease-related miRNAs depending on the network topological features without the functional similarity of miRNAs. In this paper, we develop a novel model of Integrating Network Topology Similarity and MicroRNA Function Similarity (INTS-MFS). The integrated miRNA similarities are calculated based on miRNA functional similarity… More
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