Open Access iconOpen Access

ARTICLE

crossmark

Knockdown of lncRNA XIST prevents the epithelial-mesenchymal transition of TGF-β2-induced human lens epithelial cells via miR-124/Slug axis

XUE JIANG1,2, HONG ZHANG1,2,*

1 Eye Hospital, the First Affiliated Hospital of Harbin Medical University, Harbin, 150007, China
2 Key laboratory of Basic and Clinical Research of Heilongjiang Province, Harbin, 150086, China

* Corresponding Author: HONG ZHANG. Email: email

(This article belongs to the Special Issue: Noncoding RNAs & Associated Human Diseases)

BIOCELL 2022, 46(3), 785-794. https://doi.org/10.32604/biocell.2022.016167

Abstract

Posterior capsular opacification (PCO) is linked to the pathological process of lens epithelial cells, which includes proliferation, migration, and epithelial-mesenchymal transition (EMT). Our goal was to investigate whether long noncoding RNA (lncRNA) XIST contributes to EMT via targeting miR-124/Slug axis in TGF-β2-induced SRA01/04 cells. EMT was induced by stimulating SRA01/04 cells with 10 ng/mL TGF-β2 for 24 h, and PCO microenvironment was simulated. The expressions levels of lncRNA XIST, miR-124, and Slug were measured by real-time polymerase chain reaction (RT-PCR) and western blot. The role and mechanism of lncRNA XIST in promoting EMT of TGF-β2-treated SRA01/04 cells were investigated by using cell transfection, cell counting kit-8 (CCK-8), immunofluorescence staining, transwell assay, wound-healing assay, RT-PCR, western blot and dual-luciferase reporter assay. The expression of Slug and lncRNA XIST was markedly increased, while miR-124 was downregulated in TGF-β2-treated SRA01/04 cells compared with the control group. Knockdown of lncRNA XIST reduced EMT, migration, and cell viability in TGF-β2-induced SRA01/04 cells; moreover, it adversely modulated miR-124 and adjusted the expression of Slug in SRA01/04 cells, while these effects were diminished by co-transfection with AMO-miR-124. Our data demonstrated that lncRNA XIST functioned as a competitive endogenous RNA (ceRNA) of miR-124 to modulate the expression level of Slug, thereby modulating EMT, migration, and cell viability in SRA01/04 cells. In conclusion, lncRNA XIST has a crucial role in promoting TGF-β2-induced EMT via modulating the miR-124/Slug axis in SRA01/04 cells, and it may provide a novel therapeutic option for PCO treatment.

Keywords


Cite This Article

APA Style
JIANG, X., ZHANG, H. (2022). Knockdown of lncrna XIST prevents the epithelial-mesenchymal transition of tgf-β2-induced human lens epithelial cells via mir-124/slug axis. BIOCELL, 46(3), 785-794. https://doi.org/10.32604/biocell.2022.016167
Vancouver Style
JIANG X, ZHANG H. Knockdown of lncrna XIST prevents the epithelial-mesenchymal transition of tgf-β2-induced human lens epithelial cells via mir-124/slug axis. BIOCELL . 2022;46(3):785-794 https://doi.org/10.32604/biocell.2022.016167
IEEE Style
X. JIANG and H. ZHANG, "Knockdown of lncRNA XIST prevents the epithelial-mesenchymal transition of TGF-β2-induced human lens epithelial cells via miR-124/Slug axis," BIOCELL , vol. 46, no. 3, pp. 785-794. 2022. https://doi.org/10.32604/biocell.2022.016167



cc Copyright © 2022 The Author(s). Published by Tech Science Press.
This work is licensed under a Creative Commons Attribution 4.0 International License , which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
  • 1743

    View

  • 878

    Download

  • 0

    Like

Share Link