Special Issue "Noncoding RNAs & Associated Human Diseases"

Submission Deadline: 30 April 2021 (closed)
Guest Editors

Dr. Jagpreet Nanda, National Institute of Health (NIH), USA. jagpreet.nanda@nih.gov


Dr. Shardul Kulkarni, Penn State University, USA. Smk6881@psu.edu


Dr. Abdul Khalique, National Institute of Health (NIH), USA. abdul.khalique@nih.gov

Summary

Approximately 80% of human genome is transcribed into noncoding RNAs (ncRNAs) including long noncoding RNAs (LncRNAs), small nuclear RNAs (snRNAs), small nucleolar RNAs (snoRNAs), micro RNAs (miRNAs), small interfering RNAs (siRNAs), transfer RNAs (tRNAs), ribosomal RNAs (rRNAs), and circular RNAs, that plays very crucial role in regulation of cellular function. The comparative genomics analysis of various organisms has strongly suggested that the genomic complexity of eukaryotes lies in the ncRNAs. The ncRNAs are shown to play roles in controlling the gene expression by simply base pairing with other nucleic acid targets and proteins, and can regulate transcription, RNA processing, mRNA translation and degradation. In addition to their regulatory functions on controlling coding gene expression, they can interact and regulate with each other by various molecular mechanisms generating complex network. Thus, their dysfunctions or expression variability may affect their biological roles which may cause various pathological phenotypes including neurodevelopmental disorders, metabolic diseases and cancer.  Over the past few years, lot of focus has been put on the functional role of the ncRNAs in disease progression. However, the mechanistic details of ncRNAs mediated disease progression and gene expression control still remain to be investigated. Additionally, recent reports suggest that there is a connection between ncRNAs dysfunction and Covid19 disease progression, but more research is required to unravel to link between COVID-19 biology and ncRNAs.

 

This special issue will focus on the novel findings and recent research and development in ncRNAs with respect to their regulatory functions that could associate it to the human disease progression. We invite authors to submit wide range of manuscripts, including experimental research, methodologies, short commentary and review articles pertaining to the role of ncRNAs and disease progression. 


Keywords
ncRNAs, Gene Expression Regulation, Human Diseases

Published Papers
  • Identification of key long noncoding RNAs and their biological functions in hepatocellular carcinoma
  • Abstract Long noncoding RNAs (lncRNAs) are vital regulators in tumorigenesis and metastasis. However, the pathological role of lncRNAs in hepatocellular carcinoma (HCC) is still unclear. In this study, we filtered out three lncRNAs from The Cancer Genome Atlas (TCGA) data that were screened for basic expression and clinical research. We selected lncRNA-NEAT1 for further study to explore its function in HCC progression and its regulatory mechanism. We identified three differentially expressed lncRNAs (DElncRNAs) in tumor and adjacent normal tissues from the TCGA library using data mining methods: lncRNA-NEAT1, lncRNA-MAGI2-AS3 and lncRNA-HCG11. Their basic expression levels were detected by qPCR. Then, we… More
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  • miR-181b promotes the oncogenesis of renal cell carcinoma by targeting TIMP3
  • Abstract Renal cell carcinoma (RCC) has a poor prognosis due to limited diagnosis and treatment. Thus, it is necessary to find novel prognostic biomarkers and therapeutic targets. The aberrant expression of microRNAs plays an important role in RCC oncogenesis. Tissue inhibitors of metalloproteinase 3 (TIMP3) acts as a downstream target of miR-181b. The aim of this study was to understand the role and molecular mechanism of miR-181b in RCC oncogenesis. The results showed that miR-181b expression was significantly higher in RCC tumour tissues, especially in those with significant invasion or metastasis. miR-181b overexpression promoted proliferation and migration of the RCC cell… More
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  • Dihydroartemisinin ameliorates palmitate-induced apoptosis in cardiomyocytes via regulation on miR-133b/Sirt1 axis
  • Abstract Excessive fat ectopically deposited in the non-adipose tissues is considered as one of the leading causes of myopathy. The aim of this study was to investigate the role of Dihydroartemisinin (DHA) in palmitate (PAL)-incubated H9c2 cells (lipotoxicity-induced cell injury model). Cell viability of PAL-treated cells was determined by MTT assay, and apoptotic regulators were examined by qRT-PCR and western blot analysis, in the absence or in the presence of DHA, respectively. Expression levels of miR-133b and Sirt1 were also evaluated by qRT-PCR and western blotting examination. PAL decreased the viability of H9c2 cells and enhanced the expression of apoptotic genes.… More
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  • Identification of a 10-pseudogenes signature as a novel prognosis biomarker for ovarian cancer
  • Abstract The outcomes of ovarian cancer are complicated and usually unfavorable due to their diagnoses at a late stage. Identifying the efficient prognostic biomarkers to improve the survival of ovarian cancer is urgently warranted. The survival-related pseudogenes retrieved from the Cancer Genome Atlas database were screened by univariate Cox regression analysis and further assessed by least absolute shrinkage and selection operator (LASSO) method. A risk score model based on the prognostic pseudogenes was also constructed. The pseudogene-mRNA regulatory networks were established using correlation analysis, and their potent roles in the ovarian cancer progression were uncovered by functional enrichment analysis. Lastly, ssGSEA… More
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  • Uncoupling tumor necrosis factor-α and interleukin-10 at tumor immune microenvironment of breast cancer through miR-17-5p/MALAT-1/H19 circuit
  • Abstract Triple Negative Breast Cancer (TNBC) immunotherapy has recently shown promising approach. However, some TNBC patients presented with resistance. One of the reasons was attributed to the excessive release of cytokines at the tumor microenvironment (TME) such as Tumor necrosis factor alpha (TNF-α) and Interleukin-10 (IL-10). Fine regulation of these cytokines’ levels via non-coding RNAs (ncRNAs) might alleviate the immune quiescent nature of TME at TNBC tumors. However, the extrapolation of ncRNAs as therapeutic tools is highly challenging. Therefore, disentanglement the nature for the isolation of natural compounds that could modulate the ncRNAs and their respective targets is an applicable translational… More
  •   Views:674       Downloads:412       Cited by:2        Download PDF


  • Alteration in the expression of microRNA-21 regulated target genes: Role in breast cancer
  • Abstract Breast cancer, also recognized as the principal cause of cancer-related deaths among women, is the second most familiar and prevalent form of cancer. New diagnostic and prognostic biomarkers that are highly specific are urgently needed for its early prognosis. MicroRNAs (miRNAs), a class of non-coding RNAs, are known to control the biological processes involving transcription, post-transcriptional and covalent modifications, splicing, translation, cell differentiation, proliferation, apoptosis, cancer progression, and invasion. Any dysregulation in miRNA expression, demonstrating their oncogenic and tumor-suppressive functions, contributes to cancer progression. MicroRNA-21 (miR-21), an ‘onco-miR’ in breast cancer, is involved in tumor progression and metastasis by suppressing… More
  •   Views:1018       Downloads:616       Cited by:1        Download PDF


  • Revisiting miR-155 in intervertebral disc degeneration: blood cell signature and local cell-free profiles
  • Abstract We unraveled the expression profiles of coding-noncoding RNAs in intervertebral disc degeneration (IDD). However, it remains elusive regarding miR-155 expression in peripheral blood mononuclear cells (PBMCs) and local extracellular space in IDD. The study aimed for investigating the miR-155 expression of PBMCs, extracellular miRNAs (ex-miRNAs) of human nucleus pulposus (NP) tissues, and morphological changes of cell death in the IDD process. Here, we harvested peripheral blood and NP samples from scoliosis and IDD patients as control and degenerative groups, respectively. Then standard Ficoll density-gradient centrifugation was used to isolate PBMCs. The two subpopulations of PBMCs were divided based on the… More
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  • miR-153 as biomarker for cancer—functional role as tumor suppressor
  • Abstract MicroRNA-153 (miR-153), belongs to a class of small non-coding RNA. It is a critical regulator of gene expression at the post-transcriptional level which interacts with the functional mRNA at 3’UTR region and suppresses the expression of the mRNA. More recently, it has become apparent that changes in the miR-153 expression lead to invasion, metastasis, angiogenesis and various types of tumor progression. This review summarizes the connection between dysregulation of miR-153 and various types of cancer progression. miR-153 regulates various signaling pathways to inhibit the proliferation and induce apoptosis in the cancer cell and also show synergistic activity with anticancer drugs.… More
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  • Understanding the role of miRNAs in the pathogenesis of brain arteriovenous malformations
  • Abstract Brain arteriovenous malformations (AVMs) are abnormal vessels that are prone to rupture, causing life-threatening intracerebral hemorrhage (ICH). Understanding the molecular basis of pathogenesis, timely diagnosis, and treatment of brain AVMs are some of the urgent problems in neurosurgery. MicroRNAs (miRNAs) are small endogenous RNAs that regulate gene-expression posttranscriptionally. MiRNAs are involved in almost all biological processes, including cell proliferation, apoptosis, and cell differentiation. Recent studies have shown that miRNAs can be involved in brain AVMs formation and rupture. There are also extracellular forms of miRNAs. Circulating miRNAs have been detected in the blood circulation and other body fluids. Owing to… More
  •   Views:841       Downloads:525       Cited by:1        Download PDF