Home / Journals / BIOCELL / Vol.43, No.4, 2019
Table of Content
  • Open Access

    ARTICLE

    Retrieval, reporting and methodological characteristics for systematic reviews/meta-analyses of animal models: a meta-epidemiological study

    Shuzhen SHI1, 2, Ming LIU1, 2, Wenjuan MA1, 2, Ya GAO1, 2, Long GE3, Xiping SHEN3, Jiarui WU4, Junhua ZHANG5, *, Jinhui TIAN1, 2, *
    BIOCELL, Vol.43, No.4, pp. 233-251, 2019, DOI:10.32604/biocell.2019.07624
    Abstract The study aimed to analyze the reporting and methodological quality of systematic reviews (SRs)/metaanalyses (MAs) of animal models to provide references for later studies and avoid the waste of medical resources. EMBASE and MEDLINE databases were searched from inception to November 2017, with no language restriction. Two reviewers selected inclusion dependently and extracted the basic characteristics. Review Manager 5.3, stata 12.0, and SPSS 21 software were used to conduct analyses. A total of 46 SRs/MAs were included. The results showed that the English databases with high retrieval frequency are PubMed/MEDLINE, EMBASE, and Web of Science. 67.31% (31/46) of the articles… More >

  • Open Access

    REVIEW

    Overview of genetic causes of recurrent miscarriage and the diagnostic approach

    Tarek A ATIA
    BIOCELL, Vol.43, No.4, pp. 253-262, 2019, DOI:10.32604/biocell.2019.08180
    Abstract Recurring miscarriage (RM) is a frustrating reproductive complication with variable etiology. Numerous genetic defects have been known to play a crucial role in the etiology of RM. Chromosomal abnormalities are frequently detected, while other genetic defects cannot be diagnosed through routine research, such as cryptic chromosomal anomalies, single nucleotide polymorphism, single-gene defect, and gene copy number variation. Diagnostic laboratories have recently used variable advanced techniques to detect potential genetic abnormalities in couples with RM and/or in products of conception. Here we aim to summarize the known genetic causes of RM, with a focus on the new diagnostic techniques. Knowledge of… More >

  • Open Access

    ARTICLE

    Relationship of multidrug-resistant gene and extended-spectrum carbapenem-resistance in Staphylococcus aureus

    Yuan LI1, Yonghyun LEE2, Yielhea SEO3, Youjin HWANG1, 2, *
    BIOCELL, Vol.43, No.4, pp. 263-269, 2019, DOI:10.32604/biocell.2019.07664
    Abstract The aim of this study was to determine the relationship between phenotypic antimicrobial susceptibility patterns and extended-spectrum, carbapenem-resistance genes. A total of 109 clinical Staphilococcus aureus strains were subjected to 19 antimicrobial susceptibility tests. Resistance to methicillin (mecA), penicillin (blaTEM), and tetracycline (tetM) was detected. We compared the presence of the blaTEM genes with extended-spectrum, carbapenem-related genes and identified the types of SCCmec genes. Of 109 clinical S. aureus strains, 62 (56.88%) had methicillin resistance and 60 strains carried mecA. The prevalence of blaTEM and tetM genes was 81.65% and 37.61%, respectively. The most predominant SCCmec type was SCCmec type… More >

  • Open Access

    ARTICLE

    An extract of Hypericum perforatum induces wound healing through inhibitions of Ca2+ mobilizations, mitochondrial oxidative stress and cell death in epithelial cells: Involvement of TRPM2 channels

    Fuat USLUSOY1, Mustafa NAZIROĞLU2,3,*
    BIOCELL, Vol.43, No.4, pp. 271-283, 2019, DOI:10.32604/biocell.2019.08333
    Abstract The wound is induced by several mechanical and metabolic factors. In the etiology of the wound recovery, excessive oxidative stress, calcium ion (Ca2+) influx, and apoptosis have important roles. Ca2+-permeable TRPM2 channel is activated by oxidative stress. Protective roles of Hypericum perforatum extract (HP) on the mechanical nerve injury-induced apoptosis and oxidative toxicity through regulation of TRPM2 in the experimental animals were recently reported. The potential protective roles in HP treatment were evaluated on the TRPM2-mediated cellular oxidative toxicity in the renal epithelium (MPK) cells. The cells were divided into three groups as control, wound, and wound + HP treatment… More >

  • Open Access

    ARTICLE

    Association of TRIM22 with the type 1 interferon response during primary human cytomegalovirus infection in THP-1 macrophages

    Wei LI, Huihui GAO, Ran TAO, Lifang LIU, Shiqiang SHANG*
    BIOCELL, Vol.43, No.4, pp. 285-291, 2019, DOI:10.32604/biocell.2019.08177
    Abstract As a response factor of interferon, tripartite motif (TRIM) 22 was reported to exert antiviral activity against viruses. In this study, THP-1 macrophages were infected with human cytomegalovirus (HCMV) to establish the HCMV lytic infection model. The mRNA levels of interleukin-6 (IL-6), tumor necrosis factor-alpha (TNF-α) and interferonbeta (IFN-β) were significantly up-regulated in THP-1 macrophages at different infection time and titers. Moreover, for the first time, upregulation of TRIM22 expression was found during HCMV infection at both mRNA and protein levels in THP-1 macrophages. Furthermore, IFN-β could induce TRIM22 expression in THP-1 macrophages or HCMV infected THP-1 macrophages. Depletion of… More >

  • Open Access

    ARTICLE

    Epithelial-mesenchymal transition contributes to malignant phenotypes of circulating tumor cells derived from gastric cancer

    Tiangen WU1, Tianhao BAO2,3, Daoming LIANG1,*, Lin WANG4,*
    BIOCELL, Vol.43, No.4, pp. 293-298, 2019, DOI:10.32604/biocell.2019.07841
    Abstract Circulating tumor cells (CTCs) are crucial to tumor metastasis, and they usually undergo epithelial– mesenchymal transition (EMT) in order to disseminate from the primary tumor. However, very little is currently known about the relationship between EMT and malignant phenotypes of CTCs in the context of gastric cancer. Therefore, this study aimed to investigate the contribution of EMT to malignant phenotypes of CTCs derived from gastric cancer cells. We xenografted MKN28 gastric cancer cells pretreated with transforming growth factor-beta 1 (TGFβ-1) into nude mice by intravenous injection. Next, we isolated CTCs from the blood of nude mice by gradient centrifugation and… More >

  • Open Access

    ARTICLE

    Vitamin D3 attenuates anxiety-like behavior in long-term ovariectomized rats with unpredictable mild stress

    Denis BARANENKO1, Julia FEDOTOVA1, 2, *, Annemieke Johanna Maria VAN DEN TOL3
    BIOCELL, Vol.43, No.4, pp. 299-311, 2019, DOI:10.32604/biocell.2019.08407
    Abstract The impact of various vitamin D3 (VD3) doses (1.0, 2.5, or 5 mg/kg, s.c.) in mitigating the negative consequences of chronic unpredictable mild stress (CUMS) was investigated. Adult female rats with long-term estrogen deficiency were assessed using the sucrose preference test (SPT), the elevated plus-maze (EPM), the light/dark test (LDT), and the open-field test (OFT) to measure anhedonia-like and anxiety-like behavior. The corticosterone (CS) and adrenocorticotrophic hormone (ACTH) concentrations in blood serum and the brain-derived neurotrophic factor (BDNF) expression in the hippocampus of long-term ovariectomized (OVX) rats were measured by ELISA kits and/ or western blotting. Treatment with VD3 (5.0… More >

  • Open Access

    ARTICLE

    Effects of polydatin on the proliferation, migration, and invasion of ovarian cancer

    Xiuchun ZHANG
    BIOCELL, Vol.43, No.4, pp. 313-319, 2019, DOI:10.32604/biocell.2019.07973
    Abstract To investigate the effects of polydatin on the proliferation, migration, and invasion of ovarian cancer, the change of proliferative ability, migration ability, and invasive ability of human ovarian cancer cell OVCAR-3, A2780, and HO-8910 was detected by using polydatin and up-regulating PI3K. The anticancer activity and mechanism of polydatin in ovarian cancer were analyzed. Polydatin could effectively inhibit the proliferation, migration, and invasion of OVCAR-3, A2780, and HO-8910, and inhibit the expression of PI3K protein. After the expression level of PI3K protein was up-regulated, the inhibitory effect of polydatin on the proliferative ability, migration ability, and invasive ability of OVCAR-3,… More >

  • Open Access

    ARTICLE

    p53 siRNA promotes autophagy of U2OS cells through its target gene Rap2B

    Heya QIAN1,§, Yan YAN2,§, Zhengjie SHEN1, Lixian XU1, Yun ZUO1, Tao ZHU3,*, Yanan CHEN1,*
    BIOCELL, Vol.43, No.4, pp. 321-326, 2019, DOI:10.32604/biocell.2019.07992
    Abstract The present study aims to explore the effects of p53 and its target gene Rap2B on the autophagy of U2OS cells. U2OS cells were treated with siRNA against p53, Rap2B, and PLCε. Relative expressions of p53, Rap2B, and PLCε were determined using quantitative polymerase chain reaction (qPCR) and Western blotting, respectively. Levels of IP3 in the cells were determined using Enzyme-linked Immunosorbent Assay (ELISA). Levels of Ca2+ were detected using Flow cytometry. Fluorescence microscopy was used to observe the autophagy of cells. Knockdown of p53 significantly decreased the expressions of Rap2B protein. Additionally, knockdown of p53 significantly decreased the mRNA… More >

  • Open Access

    ARTICLE

    Long term diazotrophic cultivation induces phycobiliprotein production in Anabaena variabilis IMU8

    Mohammed Fadhil HADDAD1,2,3, Tugba DAYIOGLU1,2, Barbaros NALBANTOĞLU2, Turgay CAKMAK1,*
    BIOCELL, Vol.43, No.4, pp. 327-333, 2019, DOI:10.32604/biocell.2019.08522
    Abstract Cyanobacteria are considered as a sustainable feedstock for the production of biochemically active compounds such as phycobiliproteins (PBPs). In this study, the impact of nitrogen (N) and phosphorus (P) availability on PBP production of “N-free acclimated” Anabaena variabilis IMU8 was analyzed. Upon isolation and identification, the cyanobacterium has been maintained in N-free BG-11 medium for more than 20 months. For experimentation, the strain was incubated in N-replete, N-depleted, N-P-depleted BG-11 medium. Long-term diazotrophic cultivation of A. variabilis IMU8 resulted in elevated PBP productivity with a limited impact on growth. When compared to N-depleted ones, N supply stimulated a slight induction… More >

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