High passage numbers induce resistance to apoptosis in C2C12 muscle cells
LUCIA PRONSATO, ANABELA LA COLLA, ANA CAROLINA RONDA, LORENA MILANESI, RICARDO BOLAND, ANDREA VASCONSUELO*
BIOCELL, Vol.37, No.1, pp. 1-9, 2013, DOI:10.32604/biocell.2013.37.001
Abstract Cell lines with high passage numbers exhibit alterations in cell morphology and functions. In the
present work, C2C12 skeletal muscle cells with either low (<20) or high (>60) passage numbers (identified as lC2C12 or h-C2C12, respectively) were used to investigate the apoptotic response to H2
O2
as a function of
culture age h-C2C12. We found that older cultures (h-C2C12 group) were depleted of mitochondrial DNA
(mtDNA). When we analyzed the behavior of Bad, Bax, caspase-3 and mitochondrial transmembrane potential,
we observed that cells in the h-C2C12 group were resistant to H2
O2 induction of apoptosis. We propose serially
cultured C2C12… More >
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