Home / Journals / BIOCELL / Vol.37, No.1, 2013
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  • Open Access

    ARTICLE

    High passage numbers induce resistance to apoptosis in C2C12 muscle cells

    LUCIA PRONSATO, ANABELA LA COLLA, ANA CAROLINA RONDA, LORENA MILANESI, RICARDO BOLAND, ANDREA VASCONSUELO*
    BIOCELL, Vol.37, No.1, pp. 1-9, 2013, DOI:10.32604/biocell.2013.37.001
    Abstract Cell lines with high passage numbers exhibit alterations in cell morphology and functions. In the present work, C2C12 skeletal muscle cells with either low (<20) or high (>60) passage numbers (identified as lC2C12 or h-C2C12, respectively) were used to investigate the apoptotic response to H2 O2 as a function of culture age h-C2C12. We found that older cultures (h-C2C12 group) were depleted of mitochondrial DNA (mtDNA). When we analyzed the behavior of Bad, Bax, caspase-3 and mitochondrial transmembrane potential, we observed that cells in the h-C2C12 group were resistant to H2 O2 induction of apoptosis. We propose serially cultured C2C12… More >

  • Open Access

    ARTICLE

    Meta-analysis of the cell cycle related C12orf48

    LOKMAN VARISLI
    BIOCELL, Vol.37, No.1, pp. 11-16, 2013, DOI:10.32604/biocell.2013.37.011
    Abstract The cell cycle is a conserved process from yeast to mammals and focuses on mechanisms that regulate the timing and frequency of DNA replication and cell division. The temporal and spatial expression of the genes is tightly regulated to ensure accurate replication and transmission of DNA to daughter cells during the cycle. Although the genes involved in interphase are well studied, most of the genes which are involved in mitotic events still remain unidentified. Since, the discovery of mitosis related genes is still incomplete, we performed a co-expression and gene ontology analysis for revealing novel mitosis regulated genes. In this… More >

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