Open Access
ARTICLE
LUCIA PRONSATO, ANABELA LA COLLA, ANA CAROLINA RONDA, LORENA MILANESI, RICARDO BOLAND, ANDREA VASCONSUELO*
BIOCELL, Vol.37, No.1, pp. 1-9, 2013, DOI:10.32604/biocell.2013.37.001
Abstract Cell lines with high passage numbers exhibit alterations in cell morphology and functions. In the
present work, C2C12 skeletal muscle cells with either low (<20) or high (>60) passage numbers (identified as lC2C12 or h-C2C12, respectively) were used to investigate the apoptotic response to H2
O2
as a function of
culture age h-C2C12. We found that older cultures (h-C2C12 group) were depleted of mitochondrial DNA
(mtDNA). When we analyzed the behavior of Bad, Bax, caspase-3 and mitochondrial transmembrane potential,
we observed that cells in the h-C2C12 group were resistant to H2
O2 induction of apoptosis. We propose serially
cultured C2C12… More >
Open Access
ARTICLE
LOKMAN VARISLI
BIOCELL, Vol.37, No.1, pp. 11-16, 2013, DOI:10.32604/biocell.2013.37.011
Abstract The cell cycle is a conserved process from yeast to mammals and focuses on mechanisms that
regulate the timing and frequency of DNA replication and cell division. The temporal and spatial expression
of the genes is tightly regulated to ensure accurate replication and transmission of DNA to daughter cells
during the cycle. Although the genes involved in interphase are well studied, most of the genes which are
involved in mitotic events still remain unidentified. Since, the discovery of mitosis related genes is still
incomplete, we performed a co-expression and gene ontology analysis for revealing novel mitosis regulated
genes. In this… More >
Login
Register
Submit a Paper
Propose a Special lssue