Home / Journals / BIOCELL / Vol.34, No.1, 2010
Table of Content
  • Open Access

    ARTICLE

    Taxus globosa S. cell lines: Initiation, selection and characterization in terms of growth, and of baccatin III and paclitaxel production

    DULCE MA. BARRADAS-DERMITZ1,*, PATRICIA M. HAYWARD-JONES2, MARTÍN MATA-ROSAS3, BEATRIZ PALMEROSSÁNCHEZ2, OSCAR B. J. PLATAS-BARRADAS4, RODOLFO F. VELÁSQUEZ-TOLEDO2
    BIOCELL, Vol.34, No.1, pp. 1-6, 2010, DOI:10.32604/biocell.2010.34.001
    Abstract Of the initial six cell lines originating from explants of Taxus globosa, or Mexican yew (stem internode, leaves and meristematic tissue), three were selected for their microbial and oxidation resistance, two from leaves and the other from stem internode. A study of their behavior, both in terms of cell growth, and of baccatin III and paclitaxel production, was developed in suspension cultures with an initially standardized biomass (fresh weight 0.23 g/L) using modified Gamborg’s B5 medium, and an elicitor (methyl jasmonate), on either the first or seventh day of culture, at several levels (0, 0.1, 1, 10, 100 μM). In… More >

  • Open Access

    ARTICLE

    Effect of morphological heterogeneity of somatic embryos of Melia azedarach on conversion into plants

    SILVIA VILA, ANA GONZALEZ, HEBE REY AND LUIS MROGINSKI*
    BIOCELL, Vol.34, No.1, pp. 7-14, 2010, DOI:10.32604/biocell.2010.34.007
    Abstract Embryogenic cultures were initiated from immature Melia azedarach (Meliaceae) zigotic embryos. Explants were induced on Murashige and Skoog (1962) medium with 4.54 μM thidiazuron or 0.45 μM dichlorophenoxyacetic acid. After 6 weeks of culture on induction medium, somatic embryos were categorized in four morphological classes based on the presence of single or fused embryos and if they remained united or not to the original explant; that were evaluated histologically. The somatic embryos of every category were transferred, in groups or individually, on a 1/4 MS medium. Bipolar embryos, the more typically normal ones, had well defined shoot and root apical… More >

  • Open Access

    ARTICLE

    Cobalt chloride stimulates phosphoinositide 3-kinase/Akt signaling through the epidermal growth factor receptor in oral squamous cell carcinoma

    MI HEON RYU1,a, JEONG HEE PARK1,a, JI EUN PARK1, JIN CHUNG2, CHANG HUN LEE3AND HAE RYOUN PARK1*
    BIOCELL, Vol.34, No.1, pp. 15-22, 2010, DOI:10.32604/biocell.2010.34.015
    Abstract Tumor cells are often found under hypoxic conditions due to the rapid outgrowth of their vascular supply, and, in order to survive hypoxia, these cells induce numerous signaling factors. Akt is an important kinase in cell survival, and its activity is regulated by the upstream phosphoinositide 3-kinase (PI3K) and receptor tyrosine kinases (RTKs). In this study, we examined Akt activation and RTKs/PI3K/Akt signaling using the hypoxia-mimetic cobalt chloride in oral squamous carcinoma cells. Cobalt chloride increases Akt phosphorylation in both a dose- and time-dependent manner. Blocking the activation of the PI3K/Akt pathway using LY294002 abolished Akt activation in response to… More >

  • Open Access

    ARTICLE

    Leaf surfaces of Gomphrena spp. (Amaranthaceae) from Cerrado biome

    SUZANE MARGARET FANK-DE-CARVALHO, MISLÉIA RODRIGUES DE AGUIAR GOMES, PEDRO ÍTALO TANNO SILVA, SÔNIA NAIR BÁO
    BIOCELL, Vol.34, No.1, pp. 23-36, 2010, DOI:10.32604/biocell.2010.34.023
    Abstract The leaf structure and micromorphology characterize plant species and reflex its interactions with the environment. Leaf epidermis sculptures aid high transpiration plants on light reflection. The form and distribution of epicuticular wax crystalloids are important to characterize the surface. Aiming to know the micromorphology and the ultrastructure of G. arborescens, G. pohlii and G. virgata, leaves of these Cerrado native species were collected in Brasília, Distrito Federal, Brazil, at the Olympic Center of the Universidade de Brasília and at Reserva Ecológica do Roncador. Leaves of G. globosa, an Indian native species, were also studied for comparison. Leaves were fractionated, fixed… More >

  • Open Access

    ARTICLE

    Interleukin-1β regulates metalloproteinase activity and leptin secretion in a cytotrophoblast model

    VANINA ANDREA FONTANA1, MELISA SANCHEZ1, ELISA CEBRAL2 AND JUAN CARLOS CALVO1,3*
    BIOCELL, Vol.34, No.1, pp. 37-44, 2010, DOI:10.32604/biocell.2010.34.037
    Abstract Implantation is one of the most regulated processes in human reproduction, by endocrine and immunological systems. Cytokines are involved in embryo-maternal communication and an impaired balance could result in pregnancy loss. Here we investigated the effect of interleukin 1-β on the activity of two important metalloproteinases (MMP-2 and MMP-9) that are involved in extracellular matrix remodeling as well as the secretion of leptin, one of the reproductive hormones actively regulating their activity and secretion. We found that IL-1β activates matrix metalloproteinase activity as well as increases leptin secretion. We propose that this interleukin, through the regulation of leptin, in turn… More >

  • Open Access

    ARTICLE

    Secondary zoospores in the algal endoparasite Maullinia ectocarpii (Plasmodiophoromycota)

    ELISA R. PARODI1,2*, EDUARDO J. CÁCERES3, RENATO WESTERMEIER4, DIETER G. MÜLLER5
    BIOCELL, Vol.34, No.1, pp. 45-52, 2010, DOI:10.32604/biocell.2010.34.045
    Abstract The present paper deals with the ultrastructure of zoospores produced by the plasmodiophorid Maullinia ectocarpii, living in the marine algal host Ectocarpus siliculosus. The zoospores described here are very similar to secondary zoospores of Polymyxa graminis and Phagomyxa sp. (the latter an algal endoparasite, also). Our results indicate that M. ectocarpii produces two types of plasmodia, and suggest that is a species with a complete life cycle, as it is known for all the Plasmodiophormycota that have been studied. Sporogenic and sporangial plasmodia produce, respectively, primary zoospores with parallel flagella within thick walled resting sporangia, and secondary zoospores with opposite… More >

  • Open Access

    ARTICLE

    Brief Note: Isolation, culture, characterization and optimization of human corneal stem cells

    ALI M.SHARIFI1,2*, RADBOD DARABI2, AND KHOSROW JADIDI3
    BIOCELL, Vol.34, No.1, pp. 53-55, 2010, DOI:10.32604/biocell.2010.34.053
    Abstract The effects of human versus mouse EGF on cell growth and culture duration were studied to optimize a human limbal stem cells culture method for therapeutical autologous transplantation. Limbal cells were obtained by trypsin digestion and transferred to a culture medium. The time needed to reach full confluence in culture was determined. Specific antibodies to corneal stem cell marker (P63) versus corneal epithelial differentiation marker (K3) were used for histochemical determinations. A high proportion of P63 positive cells (85± 4.6%), and a correspondingly low proportion K3 positive cells (15 ± 3.8%) indicated that most cultured cells remained undifferentiated and were… More >

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