Open Access
REVIEW
GABOR XANTUS1,*, BALINT KISS2, GYULA MOLNAR2, CANDICE MATHESON3, V. ANNA GYARMATHY4, PETER L. KANIZSAI5
BIOCELL, Vol.45, No.3, pp. 445-449, 2021, DOI:10.32604/biocell.2021.014754
Abstract For about a quarter of a century, monitoring lactate levels and/or lactate clearance has been an unquestionable cornerstone in sepsis management. The elevated lactate level appeared to be an independent predictor of mortality, and the consequent metabolic acidosis was thought to explain a number of pathophysiological changes seen in septic shock. Recent physiological and clinical findings seem to challenge the adverse role of lactic acidosis in sepsis. Evidence suggests that lactate levels are not necessarily directly proportional to either tissue or cellular hypoxia, and conversely, despite high lactate levels, increased peripheral tissue oxygen pressure can be measured in adult patients… More >
Open Access
REVIEW
LI PAN1, JIAWEI LIU1, MOHAMMED HAMDY FAROUK2,*, GUIXIN QIN1,*, NAN BAO1, YUAN ZHAO1, HUI SUN1
BIOCELL, Vol.45, No.3, pp. 451-459, 2021, DOI:10.32604/biocell.2021.014289
Abstract Soybean agglutinin (SBA) is an important anti-nutritional factor in soybean. SBA can induce animal growth inhibition, cause pathological changes of intestinal tissue, and decrease in the immune system functioning. Recently, a great deal of research has been done on the effects of SBA on cell morphology, division, apoptosis, autophagy, as well as the correlated signal transduction pathway. This review mainly covers the chemical and biological characteristics of SBA, describes the multifaceted aspects of SBA anti-nutritional functions, and highlights the possible cellular and molecular mechanism of anti-nutritional effects of SBA. This review has important implications for the prevention and treatment of… More >
Open Access
REVIEW
AMYLLY SANUELLY DA PAZ MARTINS1, SAMARA BOMFIM GOMES CAMPOS2, MARÍLIA OLIVEIRA FONSECA GOULART1,2,3, FABIANA ANDRÉA MOURA4,*
BIOCELL, Vol.45, No.3, pp. 461-481, 2021, DOI:10.32604/biocell.2021.014332
Abstract Inflammatory bowel disease (IBD), which includes Crohn’s disease and ulcerative colitis, has a not yet
completely defined aetiology and is characterized by a progressive chronic inflammation that involves nitroxidative
stress and dysbiosis. Extraintestinal manifestations can occur and affect several organs, including the liver and bile
ducts, joints, skin, eyes, and less frequently, the heart, brain, and kidneys, increasing the risk of morbidity and
mortality. These repercussions may be associated with the activity or severity of IBD. The present review proposes to
report and analyse the participation of dysbiosis and nitroxidative stress in the genesis of extraintestinal
manifestations, aiming to contribute… More >
Open Access
REVIEW
AMAYNA ZAKARIA, SYED ABDULLAH IBN ASADUZZAMAN, ZOBAYDA NAHAR, HAFSA JARIN SNIGDHA, TASKINA MURSHED, RASHED NOOR*
BIOCELL, Vol.45, No.3, pp. 483-487, 2021, DOI:10.32604/biocell.2021.014704
Abstract The extent and aggression of colorectal cancer is a worldwide public health threat. Extensive research has been
conducted on the pre-requisites leading to this fatal cancer. An array of genes along with their mutations and the signal
transduction pathways leading to the cellular transformation into the cancerous cells have been investigated. Based on the
knowledge gained so far, present review shortly discussed the role of the major genes especially those are involved in
instigating abnormalities in the cellular cycles, cellular proliferation and differentiation. A simple but novel molecular
scheme of the colorectal cancer development has also been plotted. More >
Open Access
REVIEW
ZIXUAN WANG1,#, HONGMEI PENG2,#, WEI SHI4,#, LU GAN1, LIPING ZHONG1, JIAN HE1, LINLIN XIE1, PAN WU1, YONGXIANG ZHAO1, ZHIMING DENG3,*, HONGLIANG TANG4, YONG HUANG1,3,*
BIOCELL, Vol.45, No.3, pp. 489-500, 2021, DOI:10.32604/biocell.2021.014439
（This article belongs to this Special Issue: Cellular Biomechanics in Health and Diseases)
Abstract Although great achievements have been made in the past decades in medicine, cancer remains a worldwide
public health issue. Surgery is usually accompanied by shortcomings such as residual lesions and poor treatment
effects, and the successive appearance of other treatment methods, such as radiotherapy and chemotherapy, has not
changed the postoperative recurrence rate, toxicity, and side effects. However, the advent of photodynamic therapy
has greatly improved this situation. Photodynamic therapy is an emerging tumor diagnosis and treatment technology
with good application prospects, photodynamic therapy uses a specific wavelength of light to excite a photosensitizer
to generate reactive oxygen species, damage… More >
Open Access
REVIEW
KAVITHA GOVARTHANAN1,*, PIYUSH KUMAR GUPTA2, BINITA ZIPPORAHE1, REKHA GAHTORI3, SOUMYA PANDIT2, RAM PRASAD4,*
BIOCELL, Vol.45, No.3, pp. 501-515, 2021, DOI:10.32604/biocell.2021.014441
（This article belongs to this Special Issue: Stem Cells, Protein Therapeutics, and Regenerative Medicine)
Abstract Stem cells constitute the source of cells that replenishes the worn out or damaged cells in our tissue and enable
the tissue to carry out the destined function. Tissue-specific stem cells are compartmentalized in a niche, which keeps the
stem cells under quiescent condition. Thus, understanding the molecular events driving the successful differentiation of
stem cells into several lineages is essential for its better manipulation of human applications. Given the developmental
aspects of the cell, the cellular function is greatly dependent on the epigenomics signature that in turn governs the
expression profile of the cell. The stable inheritance of the… More >
Open Access
REVIEW
YASHVI SHARMA, SUCHI GUPTA, SUJATA MOHANTY*
BIOCELL, Vol.45, No.3, pp. 517-520, 2021, DOI:10.32604/biocell.2021.014621
（This article belongs to this Special Issue: Stem Cells, Protein Therapeutics, and Regenerative Medicine)
Abstract In these times of despair when a nano-sized organism, the SARS-CoV-2, has rendered the human race helpless,
made the global health status decline, and drowned the world economy, a ray of hope comes from another nano-sized
particle, the exosome. The potential of mesenchymal stem cells has already been established in COVID-19; however,
cell-based therapy has its risks. We thereby propose cell-free therapy using stem cells-derived exosomes to fight
against COVID-19, as they can be a game-changer owing to their immunomodulatory nature, which combats the
cytokine storm characterizing this disease, and their practical efficiency, which will realistically aid large access to… More >
Open Access
ARTICLE
XIAOPANG REN#, WANJIAO HAO#, JIN LIU, YE LI, BING WANG, XINHUI ZU, HUILING XUE*
BIOCELL, Vol.45, No.3, pp. 521-526, 2021, DOI:10.32604/biocell.2021.012137
（This article belongs to this Special Issue: Stem Cells, Protein Therapeutics, and Regenerative Medicine)
Abstract Ovarian cancer was one of the most common malignant tumors in female reproductive organs. Moreover,
epithelial ovarian cancer showed the highest mortality rate in gynecological tumors, posing serious threats to women’s
life and health. Transient Receptor Potential Cation Channel Subfamily V Member 2 (TRPV2) and matrix
metalloproteinase-2 MMP-2 have been found to play important roles in regulating the pathogenesis of most tumors,
but there were few studies exploring the relationships of TRPV2 and MMP-2 in OC. Therefore, we evaluated the
expression of TRPV2 and MMP-2 proteins in cancer tissues and adjacent normal tissues of OC patients.
Immunohistochemistry was used to… More >
Open Access
ARTICLE
DONG HAN KIM1, DONG RAK KWON1,*, GI-YOUNG PARK1, YONG SUK MOON2
BIOCELL, Vol.45, No.3, pp. 527-536, 2021, DOI:10.32604/biocell.2021.014350
（This article belongs to this Special Issue: Stem Cells, Protein Therapeutics, and Regenerative Medicine)
Abstract This work aimed to investigate the synergetic therapeutic effects of polydeoxyribonucleotides (PDRN)
combined with extracorporeal shock waves therapy (ESWT) and the effects of the therapy according to ESWT
sequences on a chronic traumatic full-thickness rotator cuff tear (RCT) in rabbit models. For this purpose, thirty-two
rabbits were randomly allocated into 4 groups. An excision was made to create a 5-mm sized full-thickness RCT right
proximal to the insertion site on the supraspinatus. After 6 weeks, 4 different procedures (normal saline, Group 1;
PDRN injection, Group 2; PDRN injection before ESWT, Group 3; PDRN injection after ESWT, Group 4) were
performed.… More >
Open Access
ARTICLE
JIGANG LEI1,#, ZHENYAO XU2,#, SUKE LI1, MENG LI1, ZHIKAI WANG3, PING LI1, JING WANG1, YINGLU CHEN1, XIAOLE SONG1, CHENGJIE REN1, MEIPING SHEN1, CHENGXIANG DAI1,*
BIOCELL, Vol.45, No.3, pp. 537-546, 2021, DOI:10.32604/biocell.2021.014569
（This article belongs to this Special Issue: Stem Cells, Protein Therapeutics, and Regenerative Medicine)
Abstract Psoriasis is an autoimmune-related chronic inflammatory disease with an approximate prevalence of 2–3%
around the world, involving increased keratinocyte proliferation. Indeed, Th17 cells and IL-17 play critical roles in the
pathogenesis of psoriasis. The monoclonal antibodies against cytokines have been shown to have effectively
immunosuppressive effects on human psoriasis. However, there are still some patients that have no response to these
treatments. Some patients have even serious side-effects which may affect their life. Mesenchymal stem cells have the
ability of immunosuppressive and anti-inflammatory effects, which may be an alternative therapy with more safety
and efficacy for human psoriasis. Moreover, the… More >
Open Access
ARTICLE
HSIUYING WANG1,2,*
BIOCELL, Vol.45, No.3, pp. 547-561, 2021, DOI:10.32604/biocell.2021.014343
（This article belongs to this Special Issue: Molecular and Cellular Diagnostic Models)
Abstract Amyotrophic lateral sclerosis (ALS), also called Lou Gehrig’s disease, is an irreversible disease that is caused by the degeneration and death of motor neurons. Approximately 5–10% of cases are familial ALS (fALS), and the other cases are sporadic ALS (sALS). Gene mutations have been identified both in fALS and sALS patients. In this study, we discuss the four ALS-related genes, C9orf72, SOD1, FUS, and TARDBP, and review the microRNAs (miRNAs) that are associated with ALS and other neurological disorders from the literature. A phylogenetic analysis is used to explore potential miRNAs that can be taken into account when studying the… More >
Open Access
ARTICLE
SUSHMITHA SRIRAMULU1, SARUBALA MALAYAPERUMAL1, SUMAN K. NANDY2, ANTARA BANERJEE1, MUSTHAFA MOHAMED ESSA3,4, SARAVANABABU CHIDAMBARAM5, M. WALID QORONFLEH6,7, SURAJIT PATHAK1,*
BIOCELL, Vol.45, No.3, pp. 563-576, 2021, DOI:10.32604/biocell.2021.014756
（This article belongs to this Special Issue: Molecular and Cellular Diagnostic Models)
Abstract Colon cancer is the third major cause of cancer deaths, accounting for about 8% in terms of mortality globally. The present study aims to explore the effect of silencing Astrocyte Elevated Gene-1 (AEG-1), a metastasis mediating factor, and how it interacts with Exostosin-1 (EXT-1) protein to inhibit the proliferative and invasive potential in colon cancer cells. Forward siRNA transfection was performed using AEG-1 siRNA in SW480 and SW620 colon cancer cell lines, and the expression levels of mRNA and protein were analyzed by Real-time PCR and Immunofluorescence. A simple bioinformatics approach was carried out to identify the possible interactions between… More >
Open Access
ARTICLE
BENJIANG QIAN1, XIAOYAN YING2, GUANG YANG1, HUIZHANG LI3, JIANMING TAN1,*
BIOCELL, Vol.45, No.3, pp. 577-588, 2021, DOI:10.32604/biocell.2021.014972
（This article belongs to this Special Issue: Molecular and Cellular Diagnostic Models)
Abstract This work aimed to discover new therapeutic targets in renal clear cell carcinoma by bioinformatics and detect the effect of candidate gene TRIP13 in renal cell carcinoma (RCC) cell proliferation, migration, and invasion. Differentially expressed mRNAs were screened based on The Cancer Genome Atlas (TCGA)-Kidney Renal Clear Cell Carcinoma (KIRC) databases, and functional enrichments, survival analysis, receiver operating characteristic curve (ROC), and Protein–Protein Interaction (PPI) protein interaction analysis were performed by R software to screen the candidate gene TRIP13. Then, the expression of candidate gene TRIP13 in 92 pairs of cancer and adjacent normal tissues of renal clear cell carcinoma… More >
Open Access
ARTICLE
MENGDAN WU1, MENGYAO SUN1, QINHUAI LAI1, YIN LU1, YUYIN FU1, YUJIA PENG1, WEIRONG LAI1, LISHI ZENG1, SHENGYAN ZHAO1, YUYAN LI1, ZHIXIONG ZHANG1, XIAOFENG CHEN1, FAN QIAO1, YIWEN ZHANG1,*, SHIJIE ZHOU1,2,*, LANTU GOU1, JINLIANG YANG1,2
BIOCELL, Vol.45, No.3, pp. 589-597, 2021, DOI:10.32604/biocell.2021.013882
Abstract The chemokine ligand 13-chemokine receptor 5 (CXCL13-CXCR5) axis has been characterized as a critical
tumor-promoting signaling pathway in the tumor microenvironment (TME) in multiple types of solid tumors. In this
study, we analyzed the expression profile of CXCL13 in kidney clear cell carcinoma (KIRC) and its correlation with
tumor-infiltrating immune cells (TIICs). A monoclonal antibody against CXCL13 with high affinity and purity was
generated in our lab for western blot and immunohistochemistry (IHC). Bioinformatic analysis was performed based
on bulk-seq data from the Cancer Genome Atlas (TCGA)-KIRC and single-cell RNA-seq data from scRNASeqDB
and PanglaoDB. Results showed that high CXCL13… More >
Open Access
ARTICLE
BENJIANG QIAN1, YOUFENG HUANG2, ZHENQIANG QIU2, XIAOYAN YING3, GUANG YANG3, HUIZHANG LI2,*, JIANMING TAN1,*
BIOCELL, Vol.45, No.3, pp. 599-615, 2021, DOI:10.32604/biocell.2021.014633
Abstract Tet methylcytosine dioxygenase 2 (TET2) acts as an antioncogene that is investigated in different cancers. But
the effects of TET2 in renal cell cancer (RCC) is still known little. Here, quantitative real-time PCR (qRT-PCR), Western
blot, and immunofluorescence were performed to exam gene and protein expression. Cell proliferation was measured
using Cell Counting Kit-8 (CCK-8). Transwell assay was performed to detect cell metastasis viability. Flow cytometry
was performed to analyze the cell cycle and cell apoptosis. The effects of TET2 on RCC growth in vivo was analyzed
using a mouse xenograft model.We found that TET2 and miR-200c were decreased in… More >
Open Access
ARTICLE
DAN ZHANG1, HAIJING LIU1, ZHENNAN YI2, YUANYUAN LU3, YANYAN CHEN4, WEIQIANG SU2, HUIBING LIN2, ZHIHUI ZHANG5,*, WEI LEI6,*
BIOCELL, Vol.45, No.3, pp. 617-625, 2021, DOI:10.32604/biocell.2021.012504
Abstract Chemotherapy drug resistance is the main cause leading to the relapse and metastasis of non-small cell lung
cancer (NSCLC) patients. Our study aimed to investigate the mechanism of pemetrexed resistance in NSCLC. Firstly,
the pemetrexed (PEM)-resistant PC-9 and A549 lung adenocarcinoma cell lines (PC-9/PEM and A549/PEM) were
established. The expression of thymidylate synthase (TS) in PC-9/PEM, A549/PEM, A549, and PC-9 cells were
analyzed by qRT-PCR and western blot. Then, cell viability, colony formation, migration, and invasion were
performed on PEM-resistant cells transfected with TS siRNA. The role of EGFR in PEM resistance of PEM-resistant
cells was investigated using EGFR siRNA.… More >
Open Access
ARTICLE
JUNQI GUO1,2,#, YUN YANG1,2,#, WEI ZHAO1,2, ZHONGHAI YAN3, XIA YANG4, YUNFEI YAN1,2, RUIMIN HAO1,2, JINXIA HU1,2,*, FEI JIAO1,2,*
BIOCELL, Vol.45, No.3, pp. 627-638, 2021, DOI:10.32604/biocell.2021.013496
Abstract Increasing evidence indicates that aberrant expressions of some microRNAs are associated with cancer
progression. However, the roles and biological mechanisms of miRNA-16-5p in human non-small cell lung cancer
(NSCLC) are not to be well studied. Here, we validated that the expression of miR-16-5p was decreased significantly
in NSCLC samples and cell lines. The correlation between the clinicopathological features of NSCLC and the miR-16-
5p expression showed that the expression of miR-16-5p in non-small cell lung cancer was linked with the advanced
TNM stage, positive lymph node metastasis, with short overall survival (OS). Also, a negative correlation between
miR-16-5p and Fermitin… More >
Open Access
ARTICLE
JI ZHU#, XIYING BO#, GENGXI JIANG, SHIHUA YAO, TIEJUN ZHAO*, LING CHEN*
BIOCELL, Vol.45, No.3, pp. 639-647, 2021, DOI:10.32604/biocell.2021.012847
Abstract Non-small cell lung cancer (NSCLC) cells intake and consume glucose at high efficiency by aerobic glycolysis to
maintain robust cell growth and resist cell death. MicroRNAs (miRNAs) have been known to play pivotal roles in NSCLC
development partly through mediating glycolysis. However, only a few miRNAs have been experimentally confirmed as
critical regulators of glycolysis in NSCLC. TCGA datasets were analyzed to screen for differentially expressed miRNAs
between NSCLC and normal tissues. The function of miR-1294-5p was determined in NSCLC cells by cell
proliferation, glucose uptake, lactate release, and Extracellular Acidification Rate (ECAR) assays. The target of miR-
1294-5p was… More >
Open Access
ARTICLE
LITING YOU1,#, FEIFEI NA2,#, JUAN ZHOU3, LIN JIAO3, YI ZHOU3,*, BINWU YING3,*
BIOCELL, Vol.45, No.3, pp. 649-663, 2021, DOI:10.32604/biocell.2021.013978
Abstract Reviews The Dectin-1 cluster comprises seven members: CLEC-12A, CLEC-12B, CLEC-1A, CLEC-7A, CLEC-
2, CLEC-9A and OLR1. These members have been demonstrated to be involved in the tumorigenesis, progression, and
metastasis of several cancers. However, little is known about their roles in human lung adenocarcinoma (LUAD). The
expression patterns of the Dectin-1 cluster were analyzed via the ONCOMINE and GEPIA databases. We evaluated
the prognostic value of the Dectin-1 cluster in patients with LUAD using the Kaplan-Meier plotter and GEPIA.
Differential expression was validated with the EMBL-EBI database, and protein expression was analyzed with the
HPA database. In addition, protein-protein interaction… More >
Open Access
ARTICLE
RUCHI SHAH1,*, SONALI VERMA1, AMRITA BHAT1, GH RASOOL BHAT1, VARUN SHARMA1, INDU SHARMA1, HEMENDER SINGH1, SANDEEP KAUL2, EKTA RAI1, SWARKAR SHARMA1,*
BIOCELL, Vol.45, No.3, pp. 665-670, 2021, DOI:10.32604/biocell.2021.09629
Abstract Esophageal cancer is the second most common type of cancer after lung carcinoma in the state of Jammu and
Kashmir (J&K). The understanding of genetics in Esophageal cancer development is poor in the state. Genome wide
association studies (GWAS) has proved to be unsurpassed tool in identification of new loci associated with different
cancers. GWAS in Chinese population has identified SNP rs2294693 present in UNC5CL (UNC-5 Family C-Terminal
like) to be associated with non-cardia gastric cancer. We performed a case control association study and genotyped
the SNP rs2294693 using Taqman allele discrimination assay in 566 individuals (166 esophageal cancer patients… More >
Open Access
ARTICLE
DILIXIATI JINSIHAN, DAN LI, MINGSHUAI ZHANG, JINCHUN FENG, QIAN ZHAO*
BIOCELL, Vol.45, No.3, pp. 671-684, 2021, DOI:10.32604/biocell.2021.012519
Abstract Hypoxia affects the advancement, metastasis, and metabolism of breast cancer (BC). The circular RNA
ribonuclease P RNA component H1 (circRPPH1) (has_circ_0000515) is implicated in tumor progression.
Nevertheless, the regulatory mechanism related to circRPPH1 in hypoxia-mediated triple-negative breast cancer
(TNBC) progression is indistinct. The expression levels of circRPPH1, miR-1296-5p, tripartite motif-containing 14
(TRIM14) mRNA in tissue samples and cells were examined through quantitative real-time polymerase chain reaction
(qRT-PCR). Cell viability, migration, and invasion were determined with Cell Counting Kit-8 (CCK-8) or transwell
assays. The levels of glucose consumption and lactate production were assessed via the Glucose Assay Kit or Lactate
Assay… More >
Open Access
ARTICLE
YANFEI LI1, LULU DAI2, KE CAI2, YINGKUI SONG2, XIQING LIU3,*
BIOCELL, Vol.45, No.3, pp. 685-694, 2021, DOI:10.32604/biocell.2021.013612
Abstract RPA3 (Replication Protein A3) (14 kD) is a part of the canonical heterotrimeric replication protein A complex
(RPA/RP-A). This study aimed to explore the functional role of RPA3 and the mechanisms of its dysregulation in breast
cancer. Data from the Cancer Genome Atlas (TCGA)-breast cancer patients and GSE75688 were utilized for gene
expression and survival analysis. Breast cancer cell lines MDA-MB-231 and SK-BR-3 were used for in-vitro cell
studies. Clonogenic assay and immunofluorescent staining of γ-H2AX were performed to examine radiation-induced
cytotoxicity. Systemic correlation analysis was performed to identify potential transcription factors (TFs) regulating
RPA3 expression. ChIP-qPCR and dual-luciferase assay… More >
Open Access
ARTICLE
MAYSON H. ALKHATIB1,2,*, SALWA M. AL-HASHEMI1, HANA M. GASHLAN1
BIOCELL, Vol.45, No.3, pp. 695-703, 2021, DOI:10.32604/biocell.2021.014349
Abstract Incorporation of etoposide (ETP) into nanoemulsion (NE) containing polyunsaturated fatty acids (PUFAs) may
potentially augment its antiproliferation effect on the cancer cells. The current study aimed to examine the in vitro antitumor
activity of a novel formulation (ETP-BC/EP-NE) produced by combining the anticancer drug (ETP) with NE (BC/EP-NE)
consisting of the black currant seed and organic evening primrose oils. The produced formulas were physically characterized
using zetasizer measurements. Their cytotoxic effect was testified at concentrations ranges from 0.0001 to 5 μM using
CCK-8. Apoptotic and anti-invasion effects were evaluated using the assays of mitochondrial membrane potential,
annexin V-FITC double staining,… More >
Open Access
ARTICLE
TIECHENG SUN1,#, YANDONG ZHANG1,#, JIANHUA LI3,#, HONG YU1, LINGLI SONG1, XINPING SUN1, XI CHEN2,*, LI TIAN1,2,*
BIOCELL, Vol.45, No.3, pp. 705-710, 2021, DOI:10.32604/biocell.2021.014694
Abstract This study aims to investigate the protective effects of peroxiredoxin 6 on the total motility and progressive
motility of human spermatozoa. Semen samples with normal parameters were collected from 23 males and
supplemented with different concentrations of peroxiredoxin 6. All the semen samples were measured according to
the WHO 5th manual, and the motile spermatozoa were extracted using IVF fertilization medium supplemented with
different peroxiredoxin 6 concentrations. Total motility and progressive motility were observed at different timepoints of culture at room temperature. After peroxiredoxin 6 supplementation, all groups had a significant increase in
total motility and progressive motility compared to… More >
Open Access
ARTICLE
HONG ZHOU1,*, XIAOFENG JIA1,2, FAN YANG1
BIOCELL, Vol.45, No.3, pp. 711-722, 2021, DOI:10.32604/biocell.2021.014193
Abstract Uncontrolled proliferation is a hallmark of cancer cells, yet the molecular mechanisms that contribute to this
proliferation are unclear. Therapeutic treatment of cancer is suboptimal in many cases, with no accurate index by which
to evaluate the success of treatment or patient prognosis. In this study, we explored the protein levels of nuclear phosphoeIF4E in acute myeloid leukemia (AML) cell lines and primary leukemia samples by Western blot and
immunofluorescence and as well analyzed transcriptomes by RNA-seq. We found nuclear phospho-eIF4E, an exporter of
oncogenic mRNAs, to be abundant in AML. Further, nuclear phospho-eIF4E abundance was significantly associated with
tumor… More >
Open Access
ARTICLE
JINGRONG SONG#, JUAN KANG#, WEI LV, YAN DONG, XIAOYING ZHANG*
BIOCELL, Vol.45, No.3, pp. 723-731, 2021, DOI:10.32604/biocell.2021.014453
Abstract Bronchial asthma is a common chronic inflammatory disease characterized by airway hyperresponsiveness (AHR),
inflammatory cell infiltration, and airway remodeling. F-box/WD repeat-containing protein 7 (FBXW7), an E3 ubiquitin
ligase, is required for various endothelial functions, such as cell migration, inflammation, and endothelial integrity. This
study aimed to investigate the role of FBXW7 in lipopolysaccharide (LPS)-induced epithelial barrier impairment in
bronchial epithelial cells in vitro. By using lentivirus-based technology, FBXW7 was overexpressed or silenced (24 h) in
human bronchial epithelial (16HBE) cells, which were treated with LPS or not (24 h). Immunoprecipitation (IP) detection
and Western blot analysis were used to evaluate… More >
Open Access
ARTICLE
YUAN SONG1,#, CAI WEI2,#, JINGJING WANG3,*
BIOCELL, Vol.45, No.3, pp. 733-744, 2021, DOI:10.32604/biocell.2021.013236
Abstract Oxidative stress-mediated cell death in cardiomyocytes contributes to the development of atrial fibrillation.
However, the detailed mechanisms are still unclear. In the present study, we established atrial fibrillation models in
mice. The cardiomyocytes were isolated from atrial fibrillation mice and normal mice and were cultured in vitro,
respectively. The results showed that cell proliferation and viability in cardiomyocytes with atrial fibrillation were
significantly lower than the cells from the normal mice. Consistently, atrial fibrillation cardiomyocytes were prone to
suffer from apoptotic cell death. Also, the oxidative stress and ferroptosis-associated signatures were significantly
increased in atrial fibrillation cardiomyocytes compared to normal… More >
Open Access
ARTICLE
ZHAOHUI HU1,2, XIANGJUN DING3, YUYAO JI2, XIAOHONG LIU4,*, ZHIWEN DING2,*
BIOCELL, Vol.45, No.3, pp. 745-749, 2021, DOI:10.32604/biocell.2021.013293
Abstract Apurine/pyrimidine-free endonuclease 1 (APEX1) is a multifunctional enzyme that contributes to oxidizationmediated DNA-cleaved base excision repair and redox activation of transcription factors. However, the role of APEX1
during cardiomyocyte oxidative stress injury is not completely understood. In the present study, whether
APEX1 protects oxidative damage-induced cardiomyocytes was investigated. mRNA and protein expression levels of
APEX1 were downregulated in the mouse model of cardiac ischemia-reperfusion injury. Furthermore, the expression
of APEX1 in hydrogen peroxide (H2O2)-treated neonatal mice cardiomyocytes was also decreased. APEX1 knockdown
aggravated H2O2-treated cardiomyocyte apoptosis indexes. By contrast, APEX1 overexpression reversed H2O2-induced
oxidative damage, as demonstrated by decreased caspase… More >
Open Access
ARTICLE
SAUD BAWAZER1, ASGHAR KHAN2, ABDUR RAUF3,*, TAIBI BEN HADDA4, YAHYA S. AL-AWTHAN5,6, OMAR BAHATTAB5, UMER RASHID7, INAMULLAH KHAN8, MUHAMMAD ASIF NAWAZ9, MD SAHAB UDDIN10,11, OLATUNDE AHMED12, MOHAMMAD ALI SHARIATI13
BIOCELL, Vol.45, No.3, pp. 751-759, 2021, DOI:10.32604/biocell.2021.014004
Abstract Protein tyrosine phosphatase 1B (PTP1B) inhibition is considered as a potential therapeutic for the treatment of cancer, type 2 diabetes, and obesity. In our present work, we investigated the anti-diabetic potential of 8-hydroxydiospyrin (8-HDN) from D. lotus against the PTP1B enzyme. It showed significant inhibitory activity of PTP1B with an IC50 value of 18.37 ± 0.02 μM. A detailed molecular docking study was carried out to analyze the binding orientation, binding energy, and mechanism of inhibition. A comparative investigation of 8-HDN in the catalytic, as well as the allosteric site of PTP1B, was performed. Binding energy data showed that compound… More >
Open Access
ARTICLE
LINGHUI YU#, RONG SHEN#, YANAN GUO, YANXUAN GUO, CHEN LI, YANFENG SONG*, DEGUI WANG*
BIOCELL, Vol.45, No.3, pp. 761-772, 2021, DOI:10.32604/biocell.2021.014602
Abstract Various factors can induce cell degeneration by altering the phenotype and metabolism of cells. Mitochondria play an essential role in cellular homeostasis and function, rendering aging processes highly associated with mitochondrial function and status. Herein, we describe an aging-prone phenotype of murine skin cells caused by depletion of Rad6B (Ube2b), an E2 ubiquitin-conjugating enzyme. In this study, using Masson’s trichrome, we showed that loss of Rad6B causes physiological structure changes in mouse skin with age. In addition, a combination of western blotting experiments, transmission electron microscopy and employment of immunofluorescence staining revealed that depletion of Rad6B was characterized by an… More >
Open Access
ARTICLE
GALINA N. DENISOVA1, IVAN D. DIMOV1, ANNA V. ZAITSEVA1,2, LINARD J. ARTIUX3, ALEXANDER A. MIRONOV1,4,*, NATALIA R. KARELINA1
BIOCELL, Vol.45, No.3, pp. 773-783, 2021, DOI:10.32604/biocell.2021.014233
Abstract Overloading the intestine enterocytes with lipids induced alteration of the Golgi complex (GC; ) and could cause glycosylation errors. Here, using differentiated Caco-2 cells with the established 0[I] blood group phenotype (no expression of the blood antigens A and B [AgA, AgB] under normal conditions) as a model of human enterocytes we examined whether the overloading of these cells with lipids could cause errors in the Golgi-dependent glycosylation. We demonstrated that under these conditions, there were alterations of the GC and the appearance of lipid droplets in the cytoplasm. Rare cells produced AgA and AgB. This suggested that after overloading… More >
Open Access
ARTICLE
FANGLIAN HE1, WEIQING DONG1,*, SHAOLONG WEI2,*, ZUYANG QIU3, JINGLI HUANG4, HUIPING JIANG1, SHIYU HUANG1, LILI LIU3
BIOCELL, Vol.45, No.3, pp. 785-796, 2021, DOI:10.32604/biocell.2021.014418
Abstract Taro (Colocasia esculenta (L.) Schott) is an important crop in Africa, Southeast Asia, and subtropics and is used as a food and medicine. The purple color pigmentation is an appealing character in taro. We sampled taro corms of the cultivar ‘Lipu Taro’ at four developmental stages, including LPYS1 (without purple pigment, 50 days of development (DOD)), LPYS2 (very few purple pigments, 75 DOD), LPYS3 (moderate purple pigments, 115 DOD) and LPYS4 (high purple pigments, 205 DOD). The purpose of our study was to identify the key genes underpinning the purple pigmentation in taro based on RNA-sequencing. Through RNA-Seq, 6453 differentially… More >
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