Home / Journals / BIOCELL / Vol.45, No.3, 2021
Table of Content
  • Open Access

    REVIEW

    Lactate reloaded–reevaluation of the importance of lactate monitoring in the management of adult sepsis in the emergency department

    GABOR XANTUS1,*, BALINT KISS2, GYULA MOLNAR2, CANDICE MATHESON3, V. ANNA GYARMATHY4, PETER L. KANIZSAI5
    BIOCELL, Vol.45, No.3, pp. 445-449, 2021, DOI:10.32604/biocell.2021.014754
    Abstract For about a quarter of a century, monitoring lactate levels and/or lactate clearance has been an unquestionable cornerstone in sepsis management. The elevated lactate level appeared to be an independent predictor of mortality, and the consequent metabolic acidosis was thought to explain a number of pathophysiological changes seen in septic shock. Recent physiological and clinical findings seem to challenge the adverse role of lactic acidosis in sepsis. Evidence suggests that lactate levels are not necessarily directly proportional to either tissue or cellular hypoxia, and conversely, despite high lactate levels, increased peripheral tissue oxygen pressure can be measured in adult patients… More >

  • Open Access

    REVIEW

    Anti-nutritional characteristics and mechanism of soybean agglutinin

    LI PAN1, JIAWEI LIU1, MOHAMMED HAMDY FAROUK2,*, GUIXIN QIN1,*, NAN BAO1, YUAN ZHAO1, HUI SUN1
    BIOCELL, Vol.45, No.3, pp. 451-459, 2021, DOI:10.32604/biocell.2021.014289
    Abstract Soybean agglutinin (SBA) is an important anti-nutritional factor in soybean. SBA can induce animal growth inhibition, cause pathological changes of intestinal tissue, and decrease in the immune system functioning. Recently, a great deal of research has been done on the effects of SBA on cell morphology, division, apoptosis, autophagy, as well as the correlated signal transduction pathway. This review mainly covers the chemical and biological characteristics of SBA, describes the multifaceted aspects of SBA anti-nutritional functions, and highlights the possible cellular and molecular mechanism of anti-nutritional effects of SBA. This review has important implications for the prevention and treatment of… More >

  • Open Access

    REVIEW

    Extraintestinal manifestations of inflammatory bowel disease, nitroxidative stress and dysbiosis: What is the link between them?

    AMYLLY SANUELLY DA PAZ MARTINS1, SAMARA BOMFIM GOMES CAMPOS2, MARÍLIA OLIVEIRA FONSECA GOULART1,2,3, FABIANA ANDRÉA MOURA4,*
    BIOCELL, Vol.45, No.3, pp. 461-481, 2021, DOI:10.32604/biocell.2021.014332
    Abstract Inflammatory bowel disease (IBD), which includes Crohn’s disease and ulcerative colitis, has a not yet completely defined aetiology and is characterized by a progressive chronic inflammation that involves nitroxidative stress and dysbiosis. Extraintestinal manifestations can occur and affect several organs, including the liver and bile ducts, joints, skin, eyes, and less frequently, the heart, brain, and kidneys, increasing the risk of morbidity and mortality. These repercussions may be associated with the activity or severity of IBD. The present review proposes to report and analyse the participation of dysbiosis and nitroxidative stress in the genesis of extraintestinal manifestations, aiming to contribute… More >

  • Open Access

    REVIEW

    A short review of the genes involved in the development and progression of colorectal cancer

    AMAYNA ZAKARIA, SYED ABDULLAH IBN ASADUZZAMAN, ZOBAYDA NAHAR, HAFSA JARIN SNIGDHA, TASKINA MURSHED, RASHED NOOR*
    BIOCELL, Vol.45, No.3, pp. 483-487, 2021, DOI:10.32604/biocell.2021.014704
    Abstract The extent and aggression of colorectal cancer is a worldwide public health threat. Extensive research has been conducted on the pre-requisites leading to this fatal cancer. An array of genes along with their mutations and the signal transduction pathways leading to the cellular transformation into the cancerous cells have been investigated. Based on the knowledge gained so far, present review shortly discussed the role of the major genes especially those are involved in instigating abnormalities in the cellular cycles, cellular proliferation and differentiation. A simple but novel molecular scheme of the colorectal cancer development has also been plotted. More >

  • Open Access

    REVIEW

    Application of photodynamic therapy in cancer: challenges and advancements

    ZIXUAN WANG1,#, HONGMEI PENG2,#, WEI SHI4,#, LU GAN1, LIPING ZHONG1, JIAN HE1, LINLIN XIE1, PAN WU1, YONGXIANG ZHAO1, ZHIMING DENG3,*, HONGLIANG TANG4, YONG HUANG1,3,*
    BIOCELL, Vol.45, No.3, pp. 489-500, 2021, DOI:10.32604/biocell.2021.014439
    (This article belongs to this Special Issue: Cellular Biomechanics in Health and Diseases)
    Abstract Although great achievements have been made in the past decades in medicine, cancer remains a worldwide public health issue. Surgery is usually accompanied by shortcomings such as residual lesions and poor treatment effects, and the successive appearance of other treatment methods, such as radiotherapy and chemotherapy, has not changed the postoperative recurrence rate, toxicity, and side effects. However, the advent of photodynamic therapy has greatly improved this situation. Photodynamic therapy is an emerging tumor diagnosis and treatment technology with good application prospects, photodynamic therapy uses a specific wavelength of light to excite a photosensitizer to generate reactive oxygen species, damage… More >

  • Open Access

    REVIEW

    Epigenetic regulation−The guardian of cellular homeostasis and lineage commitment

    KAVITHA GOVARTHANAN1,*, PIYUSH KUMAR GUPTA2, BINITA ZIPPORAHE1, REKHA GAHTORI3, SOUMYA PANDIT2, RAM PRASAD4,*
    BIOCELL, Vol.45, No.3, pp. 501-515, 2021, DOI:10.32604/biocell.2021.014441
    (This article belongs to this Special Issue: Stem Cells, Protein Therapeutics, and Regenerative Medicine)
    Abstract Stem cells constitute the source of cells that replenishes the worn out or damaged cells in our tissue and enable the tissue to carry out the destined function. Tissue-specific stem cells are compartmentalized in a niche, which keeps the stem cells under quiescent condition. Thus, understanding the molecular events driving the successful differentiation of stem cells into several lineages is essential for its better manipulation of human applications. Given the developmental aspects of the cell, the cellular function is greatly dependent on the epigenomics signature that in turn governs the expression profile of the cell. The stable inheritance of the… More >

  • Open Access

    REVIEW

    Mesenchymal stem cell-derived exosome as a nano weapon to target the COVID-19 pandemic

    YASHVI SHARMA, SUCHI GUPTA, SUJATA MOHANTY*
    BIOCELL, Vol.45, No.3, pp. 517-520, 2021, DOI:10.32604/biocell.2021.014621
    (This article belongs to this Special Issue: Stem Cells, Protein Therapeutics, and Regenerative Medicine)
    Abstract In these times of despair when a nano-sized organism, the SARS-CoV-2, has rendered the human race helpless, made the global health status decline, and drowned the world economy, a ray of hope comes from another nano-sized particle, the exosome. The potential of mesenchymal stem cells has already been established in COVID-19; however, cell-based therapy has its risks. We thereby propose cell-free therapy using stem cells-derived exosomes to fight against COVID-19, as they can be a game-changer owing to their immunomodulatory nature, which combats the cytokine storm characterizing this disease, and their practical efficiency, which will realistically aid large access to… More >

  • Open Access

    ARTICLE

    Study on the clinical significance of TRPV2 and MMP2 expressions in ovarian cancer

    XIAOPANG REN#, WANJIAO HAO#, JIN LIU, YE LI, BING WANG, XINHUI ZU, HUILING XUE*
    BIOCELL, Vol.45, No.3, pp. 521-526, 2021, DOI:10.32604/biocell.2021.012137
    (This article belongs to this Special Issue: Stem Cells, Protein Therapeutics, and Regenerative Medicine)
    Abstract Ovarian cancer was one of the most common malignant tumors in female reproductive organs. Moreover, epithelial ovarian cancer showed the highest mortality rate in gynecological tumors, posing serious threats to women’s life and health. Transient Receptor Potential Cation Channel Subfamily V Member 2 (TRPV2) and matrix metalloproteinase-2 MMP-2 have been found to play important roles in regulating the pathogenesis of most tumors, but there were few studies exploring the relationships of TRPV2 and MMP-2 in OC. Therefore, we evaluated the expression of TRPV2 and MMP-2 proteins in cancer tissues and adjacent normal tissues of OC patients. Immunohistochemistry was used to… More >

  • Open Access

    ARTICLE

    Synergetic effects of shock waves with polydeoxyribonucleotides on rotator cuff tendon tear in a rabbit model

    DONG HAN KIM1, DONG RAK KWON1,*, GI-YOUNG PARK1, YONG SUK MOON2
    BIOCELL, Vol.45, No.3, pp. 527-536, 2021, DOI:10.32604/biocell.2021.014350
    (This article belongs to this Special Issue: Stem Cells, Protein Therapeutics, and Regenerative Medicine)
    Abstract This work aimed to investigate the synergetic therapeutic effects of polydeoxyribonucleotides (PDRN) combined with extracorporeal shock waves therapy (ESWT) and the effects of the therapy according to ESWT sequences on a chronic traumatic full-thickness rotator cuff tear (RCT) in rabbit models. For this purpose, thirty-two rabbits were randomly allocated into 4 groups. An excision was made to create a 5-mm sized full-thickness RCT right proximal to the insertion site on the supraspinatus. After 6 weeks, 4 different procedures (normal saline, Group 1; PDRN injection, Group 2; PDRN injection before ESWT, Group 3; PDRN injection after ESWT, Group 4) were performed.… More >

  • Open Access

    ARTICLE

    Human adipose, placenta, and umbilical cord-derived mesenchymal stem cells ameliorate imiquimod-induced psoriatic mice via reducing T cells infiltration

    JIGANG LEI1,#, ZHENYAO XU2,#, SUKE LI1, MENG LI1, ZHIKAI WANG3, PING LI1, JING WANG1, YINGLU CHEN1, XIAOLE SONG1, CHENGJIE REN1, MEIPING SHEN1, CHENGXIANG DAI1,*
    BIOCELL, Vol.45, No.3, pp. 537-546, 2021, DOI:10.32604/biocell.2021.014569
    (This article belongs to this Special Issue: Stem Cells, Protein Therapeutics, and Regenerative Medicine)
    Abstract Psoriasis is an autoimmune-related chronic inflammatory disease with an approximate prevalence of 2–3% around the world, involving increased keratinocyte proliferation. Indeed, Th17 cells and IL-17 play critical roles in the pathogenesis of psoriasis. The monoclonal antibodies against cytokines have been shown to have effectively immunosuppressive effects on human psoriasis. However, there are still some patients that have no response to these treatments. Some patients have even serious side-effects which may affect their life. Mesenchymal stem cells have the ability of immunosuppressive and anti-inflammatory effects, which may be an alternative therapy with more safety and efficacy for human psoriasis. Moreover, the… More >

  • Open Access

    ARTICLE

    Phylogenetic analysis of microRNA biomarkers for amyotrophic lateral sclerosis

    HSIUYING WANG1,2,*
    BIOCELL, Vol.45, No.3, pp. 547-561, 2021, DOI:10.32604/biocell.2021.014343
    (This article belongs to this Special Issue: Molecular and Cellular Diagnostic Models)
    Abstract Amyotrophic lateral sclerosis (ALS), also called Lou Gehrig’s disease, is an irreversible disease that is caused by the degeneration and death of motor neurons. Approximately 5–10% of cases are familial ALS (fALS), and the other cases are sporadic ALS (sALS). Gene mutations have been identified both in fALS and sALS patients. In this study, we discuss the four ALS-related genes, C9orf72, SOD1, FUS, and TARDBP, and review the microRNAs (miRNAs) that are associated with ALS and other neurological disorders from the literature. A phylogenetic analysis is used to explore potential miRNAs that can be taken into account when studying the… More >

  • Open Access

    ARTICLE

    Silencing of Astrocyte Elevated Gene-1 (AEG-1) inhibits the proliferative and invasive potential through interaction with Exostosin-1 (EXT-1) in primary and metastatic colon cancer cells

    SUSHMITHA SRIRAMULU1, SARUBALA MALAYAPERUMAL1, SUMAN K. NANDY2, ANTARA BANERJEE1, MUSTHAFA MOHAMED ESSA3,4, SARAVANABABU CHIDAMBARAM5, M. WALID QORONFLEH6,7, SURAJIT PATHAK1,*
    BIOCELL, Vol.45, No.3, pp. 563-576, 2021, DOI:10.32604/biocell.2021.014756
    (This article belongs to this Special Issue: Molecular and Cellular Diagnostic Models)
    Abstract Colon cancer is the third major cause of cancer deaths, accounting for about 8% in terms of mortality globally. The present study aims to explore the effect of silencing Astrocyte Elevated Gene-1 (AEG-1), a metastasis mediating factor, and how it interacts with Exostosin-1 (EXT-1) protein to inhibit the proliferative and invasive potential in colon cancer cells. Forward siRNA transfection was performed using AEG-1 siRNA in SW480 and SW620 colon cancer cell lines, and the expression levels of mRNA and protein were analyzed by Real-time PCR and Immunofluorescence. A simple bioinformatics approach was carried out to identify the possible interactions between… More >

  • Open Access

    ARTICLE

    TRIP13 is identified as a prognosis biomarker for renal clear cell carcinoma and promotes renal cell carcinoma cell proliferation, migration and invasion

    BENJIANG QIAN1, XIAOYAN YING2, GUANG YANG1, HUIZHANG LI3, JIANMING TAN1,*
    BIOCELL, Vol.45, No.3, pp. 577-588, 2021, DOI:10.32604/biocell.2021.014972
    (This article belongs to this Special Issue: Molecular and Cellular Diagnostic Models)
    Abstract This work aimed to discover new therapeutic targets in renal clear cell carcinoma by bioinformatics and detect the effect of candidate gene TRIP13 in renal cell carcinoma (RCC) cell proliferation, migration, and invasion. Differentially expressed mRNAs were screened based on The Cancer Genome Atlas (TCGA)-Kidney Renal Clear Cell Carcinoma (KIRC) databases, and functional enrichments, survival analysis, receiver operating characteristic curve (ROC), and Protein–Protein Interaction (PPI) protein interaction analysis were performed by R software to screen the candidate gene TRIP13. Then, the expression of candidate gene TRIP13 in 92 pairs of cancer and adjacent normal tissues of renal clear cell carcinoma… More >

  • Open Access

    ARTICLE

    Chemokine Ligand 13 Expression is Abundant in the Tumor Microenvironment and Indicates Poor Prognosis of Kidney Clear Cell Carcinoma

    MENGDAN WU1, MENGYAO SUN1, QINHUAI LAI1, YIN LU1, YUYIN FU1, YUJIA PENG1, WEIRONG LAI1, LISHI ZENG1, SHENGYAN ZHAO1, YUYAN LI1, ZHIXIONG ZHANG1, XIAOFENG CHEN1, FAN QIAO1, YIWEN ZHANG1,*, SHIJIE ZHOU1,2,*, LANTU GOU1, JINLIANG YANG1,2
    BIOCELL, Vol.45, No.3, pp. 589-597, 2021, DOI:10.32604/biocell.2021.013882
    Abstract The chemokine ligand 13-chemokine receptor 5 (CXCL13-CXCR5) axis has been characterized as a critical tumor-promoting signaling pathway in the tumor microenvironment (TME) in multiple types of solid tumors. In this study, we analyzed the expression profile of CXCL13 in kidney clear cell carcinoma (KIRC) and its correlation with tumor-infiltrating immune cells (TIICs). A monoclonal antibody against CXCL13 with high affinity and purity was generated in our lab for western blot and immunohistochemistry (IHC). Bioinformatic analysis was performed based on bulk-seq data from the Cancer Genome Atlas (TCGA)-KIRC and single-cell RNA-seq data from scRNASeqDB and PanglaoDB. Results showed that high CXCL13More >

  • Open Access

    ARTICLE

    Tet methylcytosine dioxygenase 2 suppresses renal cell cancer proliferation and metastasis by regulating the miR-200c-SCD axis

    BENJIANG QIAN1, YOUFENG HUANG2, ZHENQIANG QIU2, XIAOYAN YING3, GUANG YANG3, HUIZHANG LI2,*, JIANMING TAN1,*
    BIOCELL, Vol.45, No.3, pp. 599-615, 2021, DOI:10.32604/biocell.2021.014633
    Abstract Tet methylcytosine dioxygenase 2 (TET2) acts as an antioncogene that is investigated in different cancers. But the effects of TET2 in renal cell cancer (RCC) is still known little. Here, quantitative real-time PCR (qRT-PCR), Western blot, and immunofluorescence were performed to exam gene and protein expression. Cell proliferation was measured using Cell Counting Kit-8 (CCK-8). Transwell assay was performed to detect cell metastasis viability. Flow cytometry was performed to analyze the cell cycle and cell apoptosis. The effects of TET2 on RCC growth in vivo was analyzed using a mouse xenograft model.We found that TET2 and miR-200c were decreased in… More >

  • Open Access

    ARTICLE

    Thymidylate synthase confers pemetrexed resistance of non-small cell lung cancer cells by EGFR/PI3K/AKT pathway

    DAN ZHANG1, HAIJING LIU1, ZHENNAN YI2, YUANYUAN LU3, YANYAN CHEN4, WEIQIANG SU2, HUIBING LIN2, ZHIHUI ZHANG5,*, WEI LEI6,*
    BIOCELL, Vol.45, No.3, pp. 617-625, 2021, DOI:10.32604/biocell.2021.012504
    Abstract Chemotherapy drug resistance is the main cause leading to the relapse and metastasis of non-small cell lung cancer (NSCLC) patients. Our study aimed to investigate the mechanism of pemetrexed resistance in NSCLC. Firstly, the pemetrexed (PEM)-resistant PC-9 and A549 lung adenocarcinoma cell lines (PC-9/PEM and A549/PEM) were established. The expression of thymidylate synthase (TS) in PC-9/PEM, A549/PEM, A549, and PC-9 cells were analyzed by qRT-PCR and western blot. Then, cell viability, colony formation, migration, and invasion were performed on PEM-resistant cells transfected with TS siRNA. The role of EGFR in PEM resistance of PEM-resistant cells was investigated using EGFR siRNA.… More >

  • Open Access

    ARTICLE

    MiR-16-5p plays an inhibitory role in human non-small cell lung cancer through Fermitin family member 2

    JUNQI GUO1,2,#, YUN YANG1,2,#, WEI ZHAO1,2, ZHONGHAI YAN3, XIA YANG4, YUNFEI YAN1,2, RUIMIN HAO1,2, JINXIA HU1,2,*, FEI JIAO1,2,*
    BIOCELL, Vol.45, No.3, pp. 627-638, 2021, DOI:10.32604/biocell.2021.013496
    Abstract Increasing evidence indicates that aberrant expressions of some microRNAs are associated with cancer progression. However, the roles and biological mechanisms of miRNA-16-5p in human non-small cell lung cancer (NSCLC) are not to be well studied. Here, we validated that the expression of miR-16-5p was decreased significantly in NSCLC samples and cell lines. The correlation between the clinicopathological features of NSCLC and the miR-16- 5p expression showed that the expression of miR-16-5p in non-small cell lung cancer was linked with the advanced TNM stage, positive lymph node metastasis, with short overall survival (OS). Also, a negative correlation between miR-16-5p and Fermitin… More >

  • Open Access

    ARTICLE

    microR-1294-5p inhibits glycolytic metabolism of non-small cell lung cancer cells via targeting TMPRSS11B

    JI ZHU#, XIYING BO#, GENGXI JIANG, SHIHUA YAO, TIEJUN ZHAO*, LING CHEN*
    BIOCELL, Vol.45, No.3, pp. 639-647, 2021, DOI:10.32604/biocell.2021.012847
    Abstract Non-small cell lung cancer (NSCLC) cells intake and consume glucose at high efficiency by aerobic glycolysis to maintain robust cell growth and resist cell death. MicroRNAs (miRNAs) have been known to play pivotal roles in NSCLC development partly through mediating glycolysis. However, only a few miRNAs have been experimentally confirmed as critical regulators of glycolysis in NSCLC. TCGA datasets were analyzed to screen for differentially expressed miRNAs between NSCLC and normal tissues. The function of miR-1294-5p was determined in NSCLC cells by cell proliferation, glucose uptake, lactate release, and Extracellular Acidification Rate (ECAR) assays. The target of miR- 1294-5p was… More >

  • Open Access

    ARTICLE

    Expression and prognosis analyses of Dectin-1 cluster genes in patients with lung adenocarcinoma (LUAD) and the association with immune checkpoint molecules

    LITING YOU1,#, FEIFEI NA2,#, JUAN ZHOU3, LIN JIAO3, YI ZHOU3,*, BINWU YING3,*
    BIOCELL, Vol.45, No.3, pp. 649-663, 2021, DOI:10.32604/biocell.2021.013978
    Abstract Reviews The Dectin-1 cluster comprises seven members: CLEC-12A, CLEC-12B, CLEC-1A, CLEC-7A, CLEC- 2, CLEC-9A and OLR1. These members have been demonstrated to be involved in the tumorigenesis, progression, and metastasis of several cancers. However, little is known about their roles in human lung adenocarcinoma (LUAD). The expression patterns of the Dectin-1 cluster were analyzed via the ONCOMINE and GEPIA databases. We evaluated the prognostic value of the Dectin-1 cluster in patients with LUAD using the Kaplan-Meier plotter and GEPIA. Differential expression was validated with the EMBL-EBI database, and protein expression was analyzed with the HPA database. In addition, protein-protein interaction… More >

  • Open Access

    ARTICLE

    Newly identified genetic variant rs2294693 in UNC5CL gene is associated with decreased risk of esophageal carcinoma in the J&K Population–India

    RUCHI SHAH1,*, SONALI VERMA1, AMRITA BHAT1, GH RASOOL BHAT1, VARUN SHARMA1, INDU SHARMA1, HEMENDER SINGH1, SANDEEP KAUL2, EKTA RAI1, SWARKAR SHARMA1,*
    BIOCELL, Vol.45, No.3, pp. 665-670, 2021, DOI:10.32604/biocell.2021.09629
    Abstract Esophageal cancer is the second most common type of cancer after lung carcinoma in the state of Jammu and Kashmir (J&K). The understanding of genetics in Esophageal cancer development is poor in the state. Genome wide association studies (GWAS) has proved to be unsurpassed tool in identification of new loci associated with different cancers. GWAS in Chinese population has identified SNP rs2294693 present in UNC5CL (UNC-5 Family C-Terminal like) to be associated with non-cardia gastric cancer. We performed a case control association study and genotyped the SNP rs2294693 using Taqman allele discrimination assay in 566 individuals (166 esophageal cancer patients… More >

  • Open Access

    ARTICLE

    Hypoxia-associated circular RNA RPPH1 modulates triple-negative breast cancer cell growth via the miR-1296-5p/TRIM14 axis

    DILIXIATI JINSIHAN, DAN LI, MINGSHUAI ZHANG, JINCHUN FENG, QIAN ZHAO*
    BIOCELL, Vol.45, No.3, pp. 671-684, 2021, DOI:10.32604/biocell.2021.012519
    Abstract Hypoxia affects the advancement, metastasis, and metabolism of breast cancer (BC). The circular RNA ribonuclease P RNA component H1 (circRPPH1) (has_circ_0000515) is implicated in tumor progression. Nevertheless, the regulatory mechanism related to circRPPH1 in hypoxia-mediated triple-negative breast cancer (TNBC) progression is indistinct. The expression levels of circRPPH1, miR-1296-5p, tripartite motif-containing 14 (TRIM14) mRNA in tissue samples and cells were examined through quantitative real-time polymerase chain reaction (qRT-PCR). Cell viability, migration, and invasion were determined with Cell Counting Kit-8 (CCK-8) or transwell assays. The levels of glucose consumption and lactate production were assessed via the Glucose Assay Kit or Lactate Assay… More >

  • Open Access

    ARTICLE

    RPA3 is transcriptionally activated by YY1 and its depletion enhances radiosensitivity of triple-negative and HER2-positive breast cancer

    YANFEI LI1, LULU DAI2, KE CAI2, YINGKUI SONG2, XIQING LIU3,*
    BIOCELL, Vol.45, No.3, pp. 685-694, 2021, DOI:10.32604/biocell.2021.013612
    Abstract RPA3 (Replication Protein A3) (14 kD) is a part of the canonical heterotrimeric replication protein A complex (RPA/RP-A). This study aimed to explore the functional role of RPA3 and the mechanisms of its dysregulation in breast cancer. Data from the Cancer Genome Atlas (TCGA)-breast cancer patients and GSE75688 were utilized for gene expression and survival analysis. Breast cancer cell lines MDA-MB-231 and SK-BR-3 were used for in-vitro cell studies. Clonogenic assay and immunofluorescent staining of γ-H2AX were performed to examine radiation-induced cytotoxicity. Systemic correlation analysis was performed to identify potential transcription factors (TFs) regulating RPA3 expression. ChIP-qPCR and dual-luciferase assay… More >

  • Open Access

    ARTICLE

    Formulating etoposide in a nanoemulsion containing polyunsaturated fatty acids potentiates its anti-proliferation and anti-invasion activities against the ovarian cancer cells

    MAYSON H. ALKHATIB1,2,*, SALWA M. AL-HASHEMI1, HANA M. GASHLAN1
    BIOCELL, Vol.45, No.3, pp. 695-703, 2021, DOI:10.32604/biocell.2021.014349
    Abstract Incorporation of etoposide (ETP) into nanoemulsion (NE) containing polyunsaturated fatty acids (PUFAs) may potentially augment its antiproliferation effect on the cancer cells. The current study aimed to examine the in vitro antitumor activity of a novel formulation (ETP-BC/EP-NE) produced by combining the anticancer drug (ETP) with NE (BC/EP-NE) consisting of the black currant seed and organic evening primrose oils. The produced formulas were physically characterized using zetasizer measurements. Their cytotoxic effect was testified at concentrations ranges from 0.0001 to 5 μM using CCK-8. Apoptotic and anti-invasion effects were evaluated using the assays of mitochondrial membrane potential, annexin V-FITC double staining,… More >

  • Open Access

    ARTICLE

    Addition of peroxiredoxin 6 (PRDX6) to IVF fertilization medium maintains motility and longevity of human spermatozoa

    TIECHENG SUN1,#, YANDONG ZHANG1,#, JIANHUA LI3,#, HONG YU1, LINGLI SONG1, XINPING SUN1, XI CHEN2,*, LI TIAN1,2,*
    BIOCELL, Vol.45, No.3, pp. 705-710, 2021, DOI:10.32604/biocell.2021.014694
    Abstract This study aims to investigate the protective effects of peroxiredoxin 6 on the total motility and progressive motility of human spermatozoa. Semen samples with normal parameters were collected from 23 males and supplemented with different concentrations of peroxiredoxin 6. All the semen samples were measured according to the WHO 5th manual, and the motile spermatozoa were extracted using IVF fertilization medium supplemented with different peroxiredoxin 6 concentrations. Total motility and progressive motility were observed at different timepoints of culture at room temperature. After peroxiredoxin 6 supplementation, all groups had a significant increase in total motility and progressive motility compared to… More >

  • Open Access

    ARTICLE

    Elevated nuclear phospho-eIF4E body levels are associated with tumor progression and poor prognosis for acute myeloid leukemia

    HONG ZHOU1,*, XIAOFENG JIA1,2, FAN YANG1
    BIOCELL, Vol.45, No.3, pp. 711-722, 2021, DOI:10.32604/biocell.2021.014193
    Abstract Uncontrolled proliferation is a hallmark of cancer cells, yet the molecular mechanisms that contribute to this proliferation are unclear. Therapeutic treatment of cancer is suboptimal in many cases, with no accurate index by which to evaluate the success of treatment or patient prognosis. In this study, we explored the protein levels of nuclear phosphoeIF4E in acute myeloid leukemia (AML) cell lines and primary leukemia samples by Western blot and immunofluorescence and as well analyzed transcriptomes by RNA-seq. We found nuclear phospho-eIF4E, an exporter of oncogenic mRNAs, to be abundant in AML. Further, nuclear phospho-eIF4E abundance was significantly associated with tumor… More >

  • Open Access

    ARTICLE

    FBXW7 regulates epithelial barrier impairment in human bronchial epithelial cells in vitro by targeting apoptosis signal-regulating kinase1 via the p38 pathway

    JINGRONG SONG#, JUAN KANG#, WEI LV, YAN DONG, XIAOYING ZHANG*
    BIOCELL, Vol.45, No.3, pp. 723-731, 2021, DOI:10.32604/biocell.2021.014453
    Abstract Bronchial asthma is a common chronic inflammatory disease characterized by airway hyperresponsiveness (AHR), inflammatory cell infiltration, and airway remodeling. F-box/WD repeat-containing protein 7 (FBXW7), an E3 ubiquitin ligase, is required for various endothelial functions, such as cell migration, inflammation, and endothelial integrity. This study aimed to investigate the role of FBXW7 in lipopolysaccharide (LPS)-induced epithelial barrier impairment in bronchial epithelial cells in vitro. By using lentivirus-based technology, FBXW7 was overexpressed or silenced (24 h) in human bronchial epithelial (16HBE) cells, which were treated with LPS or not (24 h). Immunoprecipitation (IP) detection and Western blot analysis were used to evaluate… More >

  • Open Access

    ARTICLE

    Upregulation of miR-143-3p attenuates oxidative stress-mediated cell ferroptosis in cardiomyocytes with atrial fibrillation by degrading glutamic-oxaloacetic transaminase 1

    YUAN SONG1,#, CAI WEI2,#, JINGJING WANG3,*
    BIOCELL, Vol.45, No.3, pp. 733-744, 2021, DOI:10.32604/biocell.2021.013236
    Abstract Oxidative stress-mediated cell death in cardiomyocytes contributes to the development of atrial fibrillation. However, the detailed mechanisms are still unclear. In the present study, we established atrial fibrillation models in mice. The cardiomyocytes were isolated from atrial fibrillation mice and normal mice and were cultured in vitro, respectively. The results showed that cell proliferation and viability in cardiomyocytes with atrial fibrillation were significantly lower than the cells from the normal mice. Consistently, atrial fibrillation cardiomyocytes were prone to suffer from apoptotic cell death. Also, the oxidative stress and ferroptosis-associated signatures were significantly increased in atrial fibrillation cardiomyocytes compared to normal… More >

  • Open Access

    ARTICLE

    APEX1 protects against oxidative damage-induced cardiomyocyte apoptosis

    ZHAOHUI HU1,2, XIANGJUN DING3, YUYAO JI2, XIAOHONG LIU4,*, ZHIWEN DING2,*
    BIOCELL, Vol.45, No.3, pp. 745-749, 2021, DOI:10.32604/biocell.2021.013293
    Abstract Apurine/pyrimidine-free endonuclease 1 (APEX1) is a multifunctional enzyme that contributes to oxidizationmediated DNA-cleaved base excision repair and redox activation of transcription factors. However, the role of APEX1 during cardiomyocyte oxidative stress injury is not completely understood. In the present study, whether APEX1 protects oxidative damage-induced cardiomyocytes was investigated. mRNA and protein expression levels of APEX1 were downregulated in the mouse model of cardiac ischemia-reperfusion injury. Furthermore, the expression of APEX1 in hydrogen peroxide (H2O2)-treated neonatal mice cardiomyocytes was also decreased. APEX1 knockdown aggravated H2O2-treated cardiomyocyte apoptosis indexes. By contrast, APEX1 overexpression reversed H2O2-induced oxidative damage, as demonstrated by decreased caspase… More >

  • Open Access

    ARTICLE

    POM analysis and computational interactions of 8-hydroxydiospyrin inside active site of protein tyrosine phosphatase 1B

    SAUD BAWAZER1, ASGHAR KHAN2, ABDUR RAUF3,*, TAIBI BEN HADDA4, YAHYA S. AL-AWTHAN5,6, OMAR BAHATTAB5, UMER RASHID7, INAMULLAH KHAN8, MUHAMMAD ASIF NAWAZ9, MD SAHAB UDDIN10,11, OLATUNDE AHMED12, MOHAMMAD ALI SHARIATI13
    BIOCELL, Vol.45, No.3, pp. 751-759, 2021, DOI:10.32604/biocell.2021.014004
    Abstract Protein tyrosine phosphatase 1B (PTP1B) inhibition is considered as a potential therapeutic for the treatment of cancer, type 2 diabetes, and obesity. In our present work, we investigated the anti-diabetic potential of 8-hydroxydiospyrin (8-HDN) from D. lotus against the PTP1B enzyme. It showed significant inhibitory activity of PTP1B with an IC50 value of 18.37 ± 0.02 μM. A detailed molecular docking study was carried out to analyze the binding orientation, binding energy, and mechanism of inhibition. A comparative investigation of 8-HDN in the catalytic, as well as the allosteric site of PTP1B, was performed. Binding energy data showed that compound… More >

  • Open Access

    ARTICLE

    Loss of the ubiquitin-conjugating enzyme Rad6B disturbs mitochondrial function and cellular homeostasis in mouse skin

    LINGHUI YU#, RONG SHEN#, YANAN GUO, YANXUAN GUO, CHEN LI, YANFENG SONG*, DEGUI WANG*
    BIOCELL, Vol.45, No.3, pp. 761-772, 2021, DOI:10.32604/biocell.2021.014602
    Abstract Various factors can induce cell degeneration by altering the phenotype and metabolism of cells. Mitochondria play an essential role in cellular homeostasis and function, rendering aging processes highly associated with mitochondrial function and status. Herein, we describe an aging-prone phenotype of murine skin cells caused by depletion of Rad6B (Ube2b), an E2 ubiquitin-conjugating enzyme. In this study, using Masson’s trichrome, we showed that loss of Rad6B causes physiological structure changes in mouse skin with age. In addition, a combination of western blotting experiments, transmission electron microscopy and employment of immunofluorescence staining revealed that depletion of Rad6B was characterized by an… More >

  • Open Access

    ARTICLE

    Overloading of differentiated Caco-2 cells during lipid transcytosis induces glycosylation mistakes in the Golgi complex

    GALINA N. DENISOVA1, IVAN D. DIMOV1, ANNA V. ZAITSEVA1,2, LINARD J. ARTIUX3, ALEXANDER A. MIRONOV1,4,*, NATALIA R. KARELINA1
    BIOCELL, Vol.45, No.3, pp. 773-783, 2021, DOI:10.32604/biocell.2021.014233
    Abstract Overloading the intestine enterocytes with lipids induced alteration of the Golgi complex (GC; ) and could cause glycosylation errors. Here, using differentiated Caco-2 cells with the established 0[I] blood group phenotype (no expression of the blood antigens A and B [AgA, AgB] under normal conditions) as a model of human enterocytes we examined whether the overloading of these cells with lipids could cause errors in the Golgi-dependent glycosylation. We demonstrated that under these conditions, there were alterations of the GC and the appearance of lipid droplets in the cytoplasm. Rare cells produced AgA and AgB. This suggested that after overloading… More >

  • Open Access

    ARTICLE

    Transcriptome analysis of purple pigment formation in Colocasia esculenta

    FANGLIAN HE1, WEIQING DONG1,*, SHAOLONG WEI2,*, ZUYANG QIU3, JINGLI HUANG4, HUIPING JIANG1, SHIYU HUANG1, LILI LIU3
    BIOCELL, Vol.45, No.3, pp. 785-796, 2021, DOI:10.32604/biocell.2021.014418
    Abstract Taro (Colocasia esculenta (L.) Schott) is an important crop in Africa, Southeast Asia, and subtropics and is used as a food and medicine. The purple color pigmentation is an appealing character in taro. We sampled taro corms of the cultivar ‘Lipu Taro’ at four developmental stages, including LPYS1 (without purple pigment, 50 days of development (DOD)), LPYS2 (very few purple pigments, 75 DOD), LPYS3 (moderate purple pigments, 115 DOD) and LPYS4 (high purple pigments, 205 DOD). The purpose of our study was to identify the key genes underpinning the purple pigmentation in taro based on RNA-sequencing. Through RNA-Seq, 6453 differentially… More >

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