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REVIEW

GABOR XANTUS^1,*, BALINT KISS², GYULA MOLNAR², CANDICE MATHESON³, V. ANNA GYARMATHY⁴, PETER L. KANIZSAI⁵
BIOCELL, Vol.45, No.3, pp. 445-449, 2021, DOI:10.32604/biocell.2021.014754
Abstract For about a quarter of a century, monitoring lactate levels and/or lactate clearance has been an unquestionable cornerstone in sepsis management. The elevated lactate level appeared to be an independent predictor of mortality, and the consequent metabolic acidosis was thought to explain a number of pathophysiological changes seen in septic shock. Recent physiological and clinical findings seem to challenge the adverse role of lactic acidosis in sepsis. Evidence suggests that lactate levels are not necessarily directly proportional to either tissue or cellular hypoxia, and conversely, despite high lactate levels, increased peripheral tissue oxygen pressure can be measured in adult patients… More >

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REVIEW

LI PAN¹, JIAWEI LIU¹, MOHAMMED HAMDY FAROUK^2,*, GUIXIN QIN^1,*, NAN BAO¹, YUAN ZHAO¹, HUI SUN¹
BIOCELL, Vol.45, No.3, pp. 451-459, 2021, DOI:10.32604/biocell.2021.014289
Abstract Soybean agglutinin (SBA) is an important anti-nutritional factor in soybean. SBA can induce animal growth inhibition, cause pathological changes of intestinal tissue, and decrease in the immune system functioning. Recently, a great deal of research has been done on the effects of SBA on cell morphology, division, apoptosis, autophagy, as well as the correlated signal transduction pathway. This review mainly covers the chemical and biological characteristics of SBA, describes the multifaceted aspects of SBA anti-nutritional functions, and highlights the possible cellular and molecular mechanism of anti-nutritional effects of SBA. This review has important implications for the prevention and treatment of… More >

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AMYLLY SANUELLY DA PAZ MARTINS¹, SAMARA BOMFIM GOMES CAMPOS², MARÍLIA OLIVEIRA FONSECA GOULART^1,2,3, FABIANA ANDRÉA MOURA^4,*
BIOCELL, Vol.45, No.3, pp. 461-481, 2021, DOI:10.32604/biocell.2021.014332
Abstract Inflammatory bowel disease (IBD), which includes Crohn’s disease and ulcerative colitis, has a not yet completely defined aetiology and is characterized by a progressive chronic inflammation that involves nitroxidative stress and dysbiosis. Extraintestinal manifestations can occur and affect several organs, including the liver and bile ducts, joints, skin, eyes, and less frequently, the heart, brain, and kidneys, increasing the risk of morbidity and mortality. These repercussions may be associated with the activity or severity of IBD. The present review proposes to report and analyse the participation of dysbiosis and nitroxidative stress in the genesis of extraintestinal manifestations, aiming to contribute… More >

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1431

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REVIEW

AMAYNA ZAKARIA, SYED ABDULLAH IBN ASADUZZAMAN, ZOBAYDA NAHAR, HAFSA JARIN SNIGDHA, TASKINA MURSHED, RASHED NOOR^*
BIOCELL, Vol.45, No.3, pp. 483-487, 2021, DOI:10.32604/biocell.2021.014704
Abstract The extent and aggression of colorectal cancer is a worldwide public health threat. Extensive research has been conducted on the pre-requisites leading to this fatal cancer. An array of genes along with their mutations and the signal transduction pathways leading to the cellular transformation into the cancerous cells have been investigated. Based on the knowledge gained so far, present review shortly discussed the role of the major genes especially those are involved in instigating abnormalities in the cellular cycles, cellular proliferation and differentiation. A simple but novel molecular scheme of the colorectal cancer development has also been plotted. More >

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1088

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ZIXUAN WANG^1,#, HONGMEI PENG^2,#, WEI SHI^4,#, LU GAN¹, LIPING ZHONG¹, JIAN HE¹, LINLIN XIE¹, PAN WU¹, YONGXIANG ZHAO¹, ZHIMING DENG^3,*, HONGLIANG TANG⁴, YONG HUANG^1,3,*
BIOCELL, Vol.45, No.3, pp. 489-500, 2021, DOI:10.32604/biocell.2021.014439
（This article belongs to this Special Issue: Cellular Biomechanics in Health and Diseases)
Abstract Although great achievements have been made in the past decades in medicine, cancer remains a worldwide public health issue. Surgery is usually accompanied by shortcomings such as residual lesions and poor treatment effects, and the successive appearance of other treatment methods, such as radiotherapy and chemotherapy, has not changed the postoperative recurrence rate, toxicity, and side effects. However, the advent of photodynamic therapy has greatly improved this situation. Photodynamic therapy is an emerging tumor diagnosis and treatment technology with good application prospects, photodynamic therapy uses a specific wavelength of light to excite a photosensitizer to generate reactive oxygen species, damage… More >

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1552

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REVIEW

KAVITHA GOVARTHANAN^1,*, PIYUSH KUMAR GUPTA², BINITA ZIPPORAHE¹, REKHA GAHTORI³, SOUMYA PANDIT², RAM PRASAD^4,*
BIOCELL, Vol.45, No.3, pp. 501-515, 2021, DOI:10.32604/biocell.2021.014441
（This article belongs to this Special Issue: Stem Cells, Protein Therapeutics, and Regenerative Medicine)
Abstract Stem cells constitute the source of cells that replenishes the worn out or damaged cells in our tissue and enable the tissue to carry out the destined function. Tissue-specific stem cells are compartmentalized in a niche, which keeps the stem cells under quiescent condition. Thus, understanding the molecular events driving the successful differentiation of stem cells into several lineages is essential for its better manipulation of human applications. Given the developmental aspects of the cell, the cellular function is greatly dependent on the epigenomics signature that in turn governs the expression profile of the cell. The stable inheritance of the… More >

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1261

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REVIEW

YASHVI SHARMA, SUCHI GUPTA, SUJATA MOHANTY^*
BIOCELL, Vol.45, No.3, pp. 517-520, 2021, DOI:10.32604/biocell.2021.014621
（This article belongs to this Special Issue: Stem Cells, Protein Therapeutics, and Regenerative Medicine)
Abstract In these times of despair when a nano-sized organism, the SARS-CoV-2, has rendered the human race helpless, made the global health status decline, and drowned the world economy, a ray of hope comes from another nano-sized particle, the exosome. The potential of mesenchymal stem cells has already been established in COVID-19; however, cell-based therapy has its risks. We thereby propose cell-free therapy using stem cells-derived exosomes to fight against COVID-19, as they can be a game-changer owing to their immunomodulatory nature, which combats the cytokine storm characterizing this disease, and their practical efficiency, which will realistically aid large access to… More >

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XIAOPANG REN^#, WANJIAO HAO^#, JIN LIU, YE LI, BING WANG, XINHUI ZU, HUILING XUE^*
BIOCELL, Vol.45, No.3, pp. 521-526, 2021, DOI:10.32604/biocell.2021.012137
（This article belongs to this Special Issue: Stem Cells, Protein Therapeutics, and Regenerative Medicine)
Abstract Ovarian cancer was one of the most common malignant tumors in female reproductive organs. Moreover, epithelial ovarian cancer showed the highest mortality rate in gynecological tumors, posing serious threats to women’s life and health. Transient Receptor Potential Cation Channel Subfamily V Member 2 (TRPV2) and matrix metalloproteinase-2 MMP-2 have been found to play important roles in regulating the pathogenesis of most tumors, but there were few studies exploring the relationships of TRPV2 and MMP-2 in OC. Therefore, we evaluated the expression of TRPV2 and MMP-2 proteins in cancer tissues and adjacent normal tissues of OC patients. Immunohistochemistry was used to… More >

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DONG HAN KIM¹, DONG RAK KWON^1,*, GI-YOUNG PARK¹, YONG SUK MOON²
BIOCELL, Vol.45, No.3, pp. 527-536, 2021, DOI:10.32604/biocell.2021.014350
（This article belongs to this Special Issue: Stem Cells, Protein Therapeutics, and Regenerative Medicine)
Abstract This work aimed to investigate the synergetic therapeutic effects of polydeoxyribonucleotides (PDRN) combined with extracorporeal shock waves therapy (ESWT) and the effects of the therapy according to ESWT sequences on a chronic traumatic full-thickness rotator cuff tear (RCT) in rabbit models. For this purpose, thirty-two rabbits were randomly allocated into 4 groups. An excision was made to create a 5-mm sized full-thickness RCT right proximal to the insertion site on the supraspinatus. After 6 weeks, 4 different procedures (normal saline, Group 1; PDRN injection, Group 2; PDRN injection before ESWT, Group 3; PDRN injection after ESWT, Group 4) were performed.… More >

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JIGANG LEI^1,#, ZHENYAO XU^2,#, SUKE LI¹, MENG LI¹, ZHIKAI WANG³, PING LI¹, JING WANG¹, YINGLU CHEN¹, XIAOLE SONG¹, CHENGJIE REN¹, MEIPING SHEN¹, CHENGXIANG DAI^1,*
BIOCELL, Vol.45, No.3, pp. 537-546, 2021, DOI:10.32604/biocell.2021.014569
（This article belongs to this Special Issue: Stem Cells, Protein Therapeutics, and Regenerative Medicine)
Abstract Psoriasis is an autoimmune-related chronic inflammatory disease with an approximate prevalence of 2–3% around the world, involving increased keratinocyte proliferation. Indeed, Th17 cells and IL-17 play critical roles in the pathogenesis of psoriasis. The monoclonal antibodies against cytokines have been shown to have effectively immunosuppressive effects on human psoriasis. However, there are still some patients that have no response to these treatments. Some patients have even serious side-effects which may affect their life. Mesenchymal stem cells have the ability of immunosuppressive and anti-inflammatory effects, which may be an alternative therapy with more safety and efficacy for human psoriasis. Moreover, the… More >

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HSIUYING WANG^1,2,*
BIOCELL, Vol.45, No.3, pp. 547-561, 2021, DOI:10.32604/biocell.2021.014343
（This article belongs to this Special Issue: Molecular and Cellular Diagnostic Models)
Abstract Amyotrophic lateral sclerosis (ALS), also called Lou Gehrig’s disease, is an irreversible disease that is caused by the degeneration and death of motor neurons. Approximately 5–10% of cases are familial ALS (fALS), and the other cases are sporadic ALS (sALS). Gene mutations have been identified both in fALS and sALS patients. In this study, we discuss the four ALS-related genes, C9orf72, SOD1, FUS, and TARDBP, and review the microRNAs (miRNAs) that are associated with ALS and other neurological disorders from the literature. A phylogenetic analysis is used to explore potential miRNAs that can be taken into account when studying the… More >

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SUSHMITHA SRIRAMULU¹, SARUBALA MALAYAPERUMAL¹, SUMAN K. NANDY², ANTARA BANERJEE¹, MUSTHAFA MOHAMED ESSA^3,4, SARAVANABABU CHIDAMBARAM⁵, M. WALID QORONFLEH^6,7, SURAJIT PATHAK^1,*
BIOCELL, Vol.45, No.3, pp. 563-576, 2021, DOI:10.32604/biocell.2021.014756
（This article belongs to this Special Issue: Molecular and Cellular Diagnostic Models)
Abstract Colon cancer is the third major cause of cancer deaths, accounting for about 8% in terms of mortality globally. The present study aims to explore the effect of silencing Astrocyte Elevated Gene-1 (AEG-1), a metastasis mediating factor, and how it interacts with Exostosin-1 (EXT-1) protein to inhibit the proliferative and invasive potential in colon cancer cells. Forward siRNA transfection was performed using AEG-1 siRNA in SW480 and SW620 colon cancer cell lines, and the expression levels of mRNA and protein were analyzed by Real-time PCR and Immunofluorescence. A simple bioinformatics approach was carried out to identify the possible interactions between… More >

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1527

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ARTICLE

BENJIANG QIAN¹, XIAOYAN YING², GUANG YANG¹, HUIZHANG LI³, JIANMING TAN^1,*
BIOCELL, Vol.45, No.3, pp. 577-588, 2021, DOI:10.32604/biocell.2021.014972
（This article belongs to this Special Issue: Molecular and Cellular Diagnostic Models)
Abstract This work aimed to discover new therapeutic targets in renal clear cell carcinoma by bioinformatics and detect the effect of candidate gene TRIP13 in renal cell carcinoma (RCC) cell proliferation, migration, and invasion. Differentially expressed mRNAs were screened based on The Cancer Genome Atlas (TCGA)-Kidney Renal Clear Cell Carcinoma (KIRC) databases, and functional enrichments, survival analysis, receiver operating characteristic curve (ROC), and Protein–Protein Interaction (PPI) protein interaction analysis were performed by R software to screen the candidate gene TRIP13. Then, the expression of candidate gene TRIP13 in 92 pairs of cancer and adjacent normal tissues of renal clear cell carcinoma… More >

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1476

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MENGDAN WU¹, MENGYAO SUN¹, QINHUAI LAI¹, YIN LU¹, YUYIN FU¹, YUJIA PENG¹, WEIRONG LAI¹, LISHI ZENG¹, SHENGYAN ZHAO¹, YUYAN LI¹, ZHIXIONG ZHANG¹, XIAOFENG CHEN¹, FAN QIAO¹, YIWEN ZHANG^1,*, SHIJIE ZHOU^1,2,*, LANTU GOU¹, JINLIANG YANG^1,2
BIOCELL, Vol.45, No.3, pp. 589-597, 2021, DOI:10.32604/biocell.2021.013882
Abstract The chemokine ligand 13-chemokine receptor 5 (CXCL13-CXCR5) axis has been characterized as a critical tumor-promoting signaling pathway in the tumor microenvironment (TME) in multiple types of solid tumors. In this study, we analyzed the expression profile of CXCL13 in kidney clear cell carcinoma (KIRC) and its correlation with tumor-infiltrating immune cells (TIICs). A monoclonal antibody against CXCL13 with high affinity and purity was generated in our lab for western blot and immunohistochemistry (IHC). Bioinformatic analysis was performed based on bulk-seq data from the Cancer Genome Atlas (TCGA)-KIRC and single-cell RNA-seq data from scRNASeqDB and PanglaoDB. Results showed that high CXCL13… More >

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BENJIANG QIAN¹, YOUFENG HUANG², ZHENQIANG QIU², XIAOYAN YING³, GUANG YANG³, HUIZHANG LI^2,*, JIANMING TAN^1,*
BIOCELL, Vol.45, No.3, pp. 599-615, 2021, DOI:10.32604/biocell.2021.014633
Abstract Tet methylcytosine dioxygenase 2 (TET2) acts as an antioncogene that is investigated in different cancers. But the effects of TET2 in renal cell cancer (RCC) is still known little. Here, quantitative real-time PCR (qRT-PCR), Western blot, and immunofluorescence were performed to exam gene and protein expression. Cell proliferation was measured using Cell Counting Kit-8 (CCK-8). Transwell assay was performed to detect cell metastasis viability. Flow cytometry was performed to analyze the cell cycle and cell apoptosis. The effects of TET2 on RCC growth in vivo was analyzed using a mouse xenograft model.We found that TET2 and miR-200c were decreased in… More >

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DAN ZHANG¹, HAIJING LIU¹, ZHENNAN YI², YUANYUAN LU³, YANYAN CHEN⁴, WEIQIANG SU², HUIBING LIN², ZHIHUI ZHANG^5,*, WEI LEI^6,*
BIOCELL, Vol.45, No.3, pp. 617-625, 2021, DOI:10.32604/biocell.2021.012504
Abstract Chemotherapy drug resistance is the main cause leading to the relapse and metastasis of non-small cell lung cancer (NSCLC) patients. Our study aimed to investigate the mechanism of pemetrexed resistance in NSCLC. Firstly, the pemetrexed (PEM)-resistant PC-9 and A549 lung adenocarcinoma cell lines (PC-9/PEM and A549/PEM) were established. The expression of thymidylate synthase (TS) in PC-9/PEM, A549/PEM, A549, and PC-9 cells were analyzed by qRT-PCR and western blot. Then, cell viability, colony formation, migration, and invasion were performed on PEM-resistant cells transfected with TS siRNA. The role of EGFR in PEM resistance of PEM-resistant cells was investigated using EGFR siRNA.… More >

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1953

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JUNQI GUO^1,2,#, YUN YANG^1,2,#, WEI ZHAO^1,2, ZHONGHAI YAN³, XIA YANG⁴, YUNFEI YAN^1,2, RUIMIN HAO^1,2, JINXIA HU^1,2,*, FEI JIAO^1,2,*
BIOCELL, Vol.45, No.3, pp. 627-638, 2021, DOI:10.32604/biocell.2021.013496
Abstract Increasing evidence indicates that aberrant expressions of some microRNAs are associated with cancer progression. However, the roles and biological mechanisms of miRNA-16-5p in human non-small cell lung cancer (NSCLC) are not to be well studied. Here, we validated that the expression of miR-16-5p was decreased significantly in NSCLC samples and cell lines. The correlation between the clinicopathological features of NSCLC and the miR-16- 5p expression showed that the expression of miR-16-5p in non-small cell lung cancer was linked with the advanced TNM stage, positive lymph node metastasis, with short overall survival (OS). Also, a negative correlation between miR-16-5p and Fermitin… More >

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JI ZHU^#, XIYING BO^#, GENGXI JIANG, SHIHUA YAO, TIEJUN ZHAO^*, LING CHEN^*
BIOCELL, Vol.45, No.3, pp. 639-647, 2021, DOI:10.32604/biocell.2021.012847
Abstract Non-small cell lung cancer (NSCLC) cells intake and consume glucose at high efficiency by aerobic glycolysis to maintain robust cell growth and resist cell death. MicroRNAs (miRNAs) have been known to play pivotal roles in NSCLC development partly through mediating glycolysis. However, only a few miRNAs have been experimentally confirmed as critical regulators of glycolysis in NSCLC. TCGA datasets were analyzed to screen for differentially expressed miRNAs between NSCLC and normal tissues. The function of miR-1294-5p was determined in NSCLC cells by cell proliferation, glucose uptake, lactate release, and Extracellular Acidification Rate (ECAR) assays. The target of miR- 1294-5p was… More >

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LITING YOU^1,#, FEIFEI NA^2,#, JUAN ZHOU³, LIN JIAO³, YI ZHOU^3,*, BINWU YING^3,*
BIOCELL, Vol.45, No.3, pp. 649-663, 2021, DOI:10.32604/biocell.2021.013978
Abstract Reviews The Dectin-1 cluster comprises seven members: CLEC-12A, CLEC-12B, CLEC-1A, CLEC-7A, CLEC- 2, CLEC-9A and OLR1. These members have been demonstrated to be involved in the tumorigenesis, progression, and metastasis of several cancers. However, little is known about their roles in human lung adenocarcinoma (LUAD). The expression patterns of the Dectin-1 cluster were analyzed via the ONCOMINE and GEPIA databases. We evaluated the prognostic value of the Dectin-1 cluster in patients with LUAD using the Kaplan-Meier plotter and GEPIA. Differential expression was validated with the EMBL-EBI database, and protein expression was analyzed with the HPA database. In addition, protein-protein interaction… More >

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RUCHI SHAH^1,*, SONALI VERMA¹, AMRITA BHAT¹, GH RASOOL BHAT¹, VARUN SHARMA¹, INDU SHARMA¹, HEMENDER SINGH¹, SANDEEP KAUL², EKTA RAI¹, SWARKAR SHARMA^1,*
BIOCELL, Vol.45, No.3, pp. 665-670, 2021, DOI:10.32604/biocell.2021.09629
Abstract Esophageal cancer is the second most common type of cancer after lung carcinoma in the state of Jammu and Kashmir (J&K). The understanding of genetics in Esophageal cancer development is poor in the state. Genome wide association studies (GWAS) has proved to be unsurpassed tool in identification of new loci associated with different cancers. GWAS in Chinese population has identified SNP rs2294693 present in UNC5CL (UNC-5 Family C-Terminal like) to be associated with non-cardia gastric cancer. We performed a case control association study and genotyped the SNP rs2294693 using Taqman allele discrimination assay in 566 individuals (166 esophageal cancer patients… More >

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DILIXIATI JINSIHAN, DAN LI, MINGSHUAI ZHANG, JINCHUN FENG, QIAN ZHAO^*
BIOCELL, Vol.45, No.3, pp. 671-684, 2021, DOI:10.32604/biocell.2021.012519
Abstract Hypoxia affects the advancement, metastasis, and metabolism of breast cancer (BC). The circular RNA ribonuclease P RNA component H1 (circRPPH1) (has_circ_0000515) is implicated in tumor progression. Nevertheless, the regulatory mechanism related to circRPPH1 in hypoxia-mediated triple-negative breast cancer (TNBC) progression is indistinct. The expression levels of circRPPH1, miR-1296-5p, tripartite motif-containing 14 (TRIM14) mRNA in tissue samples and cells were examined through quantitative real-time polymerase chain reaction (qRT-PCR). Cell viability, migration, and invasion were determined with Cell Counting Kit-8 (CCK-8) or transwell assays. The levels of glucose consumption and lactate production were assessed via the Glucose Assay Kit or Lactate Assay… More >

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YANFEI LI¹, LULU DAI², KE CAI², YINGKUI SONG², XIQING LIU^3,*
BIOCELL, Vol.45, No.3, pp. 685-694, 2021, DOI:10.32604/biocell.2021.013612
Abstract RPA3 (Replication Protein A3) (14 kD) is a part of the canonical heterotrimeric replication protein A complex (RPA/RP-A). This study aimed to explore the functional role of RPA3 and the mechanisms of its dysregulation in breast cancer. Data from the Cancer Genome Atlas (TCGA)-breast cancer patients and GSE75688 were utilized for gene expression and survival analysis. Breast cancer cell lines MDA-MB-231 and SK-BR-3 were used for in-vitro cell studies. Clonogenic assay and immunofluorescent staining of γ-H2AX were performed to examine radiation-induced cytotoxicity. Systemic correlation analysis was performed to identify potential transcription factors (TFs) regulating RPA3 expression. ChIP-qPCR and dual-luciferase assay… More >

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MAYSON H. ALKHATIB^1,2,*, SALWA M. AL-HASHEMI¹, HANA M. GASHLAN¹
BIOCELL, Vol.45, No.3, pp. 695-703, 2021, DOI:10.32604/biocell.2021.014349
Abstract Incorporation of etoposide (ETP) into nanoemulsion (NE) containing polyunsaturated fatty acids (PUFAs) may potentially augment its antiproliferation effect on the cancer cells. The current study aimed to examine the in vitro antitumor activity of a novel formulation (ETP-BC/EP-NE) produced by combining the anticancer drug (ETP) with NE (BC/EP-NE) consisting of the black currant seed and organic evening primrose oils. The produced formulas were physically characterized using zetasizer measurements. Their cytotoxic effect was testified at concentrations ranges from 0.0001 to 5 μM using CCK-8. Apoptotic and anti-invasion effects were evaluated using the assays of mitochondrial membrane potential, annexin V-FITC double staining,… More >

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TIECHENG SUN^1,#, YANDONG ZHANG^1,#, JIANHUA LI^3,#, HONG YU¹, LINGLI SONG¹, XINPING SUN¹, XI CHEN^2,*, LI TIAN^1,2,*
BIOCELL, Vol.45, No.3, pp. 705-710, 2021, DOI:10.32604/biocell.2021.014694
Abstract This study aims to investigate the protective effects of peroxiredoxin 6 on the total motility and progressive motility of human spermatozoa. Semen samples with normal parameters were collected from 23 males and supplemented with different concentrations of peroxiredoxin 6. All the semen samples were measured according to the WHO 5th manual, and the motile spermatozoa were extracted using IVF fertilization medium supplemented with different peroxiredoxin 6 concentrations. Total motility and progressive motility were observed at different timepoints of culture at room temperature. After peroxiredoxin 6 supplementation, all groups had a significant increase in total motility and progressive motility compared to… More >

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HONG ZHOU^1,*, XIAOFENG JIA^1,2, FAN YANG¹
BIOCELL, Vol.45, No.3, pp. 711-722, 2021, DOI:10.32604/biocell.2021.014193
Abstract Uncontrolled proliferation is a hallmark of cancer cells, yet the molecular mechanisms that contribute to this proliferation are unclear. Therapeutic treatment of cancer is suboptimal in many cases, with no accurate index by which to evaluate the success of treatment or patient prognosis. In this study, we explored the protein levels of nuclear phosphoeIF4E in acute myeloid leukemia (AML) cell lines and primary leukemia samples by Western blot and immunofluorescence and as well analyzed transcriptomes by RNA-seq. We found nuclear phospho-eIF4E, an exporter of oncogenic mRNAs, to be abundant in AML. Further, nuclear phospho-eIF4E abundance was significantly associated with tumor… More >

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JINGRONG SONG^#, JUAN KANG^#, WEI LV, YAN DONG, XIAOYING ZHANG^*
BIOCELL, Vol.45, No.3, pp. 723-731, 2021, DOI:10.32604/biocell.2021.014453
Abstract Bronchial asthma is a common chronic inflammatory disease characterized by airway hyperresponsiveness (AHR), inflammatory cell infiltration, and airway remodeling. F-box/WD repeat-containing protein 7 (FBXW7), an E3 ubiquitin ligase, is required for various endothelial functions, such as cell migration, inflammation, and endothelial integrity. This study aimed to investigate the role of FBXW7 in lipopolysaccharide (LPS)-induced epithelial barrier impairment in bronchial epithelial cells in vitro. By using lentivirus-based technology, FBXW7 was overexpressed or silenced (24 h) in human bronchial epithelial (16HBE) cells, which were treated with LPS or not (24 h). Immunoprecipitation (IP) detection and Western blot analysis were used to evaluate… More >

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YUAN SONG^1,#, CAI WEI^2,#, JINGJING WANG^3,*
BIOCELL, Vol.45, No.3, pp. 733-744, 2021, DOI:10.32604/biocell.2021.013236
Abstract Oxidative stress-mediated cell death in cardiomyocytes contributes to the development of atrial fibrillation. However, the detailed mechanisms are still unclear. In the present study, we established atrial fibrillation models in mice. The cardiomyocytes were isolated from atrial fibrillation mice and normal mice and were cultured in vitro, respectively. The results showed that cell proliferation and viability in cardiomyocytes with atrial fibrillation were significantly lower than the cells from the normal mice. Consistently, atrial fibrillation cardiomyocytes were prone to suffer from apoptotic cell death. Also, the oxidative stress and ferroptosis-associated signatures were significantly increased in atrial fibrillation cardiomyocytes compared to normal… More >

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ZHAOHUI HU^1,2, XIANGJUN DING³, YUYAO JI², XIAOHONG LIU^4,*, ZHIWEN DING^2,*
BIOCELL, Vol.45, No.3, pp. 745-749, 2021, DOI:10.32604/biocell.2021.013293
Abstract Apurine/pyrimidine-free endonuclease 1 (APEX1) is a multifunctional enzyme that contributes to oxidizationmediated DNA-cleaved base excision repair and redox activation of transcription factors. However, the role of APEX1 during cardiomyocyte oxidative stress injury is not completely understood. In the present study, whether APEX1 protects oxidative damage-induced cardiomyocytes was investigated. mRNA and protein expression levels of APEX1 were downregulated in the mouse model of cardiac ischemia-reperfusion injury. Furthermore, the expression of APEX1 in hydrogen peroxide (H₂O₂)-treated neonatal mice cardiomyocytes was also decreased. APEX1 knockdown aggravated H₂O₂-treated cardiomyocyte apoptosis indexes. By contrast, APEX1 overexpression reversed H₂O₂-induced oxidative damage, as demonstrated by decreased caspase… More >

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SAUD BAWAZER¹, ASGHAR KHAN², ABDUR RAUF^3,*, TAIBI BEN HADDA⁴, YAHYA S. AL-AWTHAN^5,6, OMAR BAHATTAB⁵, UMER RASHID⁷, INAMULLAH KHAN⁸, MUHAMMAD ASIF NAWAZ⁹, MD SAHAB UDDIN^10,11, OLATUNDE AHMED¹², MOHAMMAD ALI SHARIATI¹³
BIOCELL, Vol.45, No.3, pp. 751-759, 2021, DOI:10.32604/biocell.2021.014004
Abstract Protein tyrosine phosphatase 1B (PTP1B) inhibition is considered as a potential therapeutic for the treatment of cancer, type 2 diabetes, and obesity. In our present work, we investigated the anti-diabetic potential of 8-hydroxydiospyrin (8-HDN) from D. lotus against the PTP1B enzyme. It showed significant inhibitory activity of PTP1B with an IC₅₀ value of 18.37 ± 0.02 μM. A detailed molecular docking study was carried out to analyze the binding orientation, binding energy, and mechanism of inhibition. A comparative investigation of 8-HDN in the catalytic, as well as the allosteric site of PTP1B, was performed. Binding energy data showed that compound… More >

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LINGHUI YU^#, RONG SHEN^#, YANAN GUO, YANXUAN GUO, CHEN LI, YANFENG SONG^*, DEGUI WANG^*
BIOCELL, Vol.45, No.3, pp. 761-772, 2021, DOI:10.32604/biocell.2021.014602
Abstract Various factors can induce cell degeneration by altering the phenotype and metabolism of cells. Mitochondria play an essential role in cellular homeostasis and function, rendering aging processes highly associated with mitochondrial function and status. Herein, we describe an aging-prone phenotype of murine skin cells caused by depletion of Rad6B (Ube2b), an E2 ubiquitin-conjugating enzyme. In this study, using Masson’s trichrome, we showed that loss of Rad6B causes physiological structure changes in mouse skin with age. In addition, a combination of western blotting experiments, transmission electron microscopy and employment of immunofluorescence staining revealed that depletion of Rad6B was characterized by an… More >

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GALINA N. DENISOVA¹, IVAN D. DIMOV¹, ANNA V. ZAITSEVA^1,2, LINARD J. ARTIUX³, ALEXANDER A. MIRONOV^1,4,*, NATALIA R. KARELINA¹
BIOCELL, Vol.45, No.3, pp. 773-783, 2021, DOI:10.32604/biocell.2021.014233
Abstract Overloading the intestine enterocytes with lipids induced alteration of the Golgi complex (GC; ) and could cause glycosylation errors. Here, using differentiated Caco-2 cells with the established 0[I] blood group phenotype (no expression of the blood antigens A and B [AgA, AgB] under normal conditions) as a model of human enterocytes we examined whether the overloading of these cells with lipids could cause errors in the Golgi-dependent glycosylation. We demonstrated that under these conditions, there were alterations of the GC and the appearance of lipid droplets in the cytoplasm. Rare cells produced AgA and AgB. This suggested that after overloading… More >

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FANGLIAN HE¹, WEIQING DONG^1,*, SHAOLONG WEI^2,*, ZUYANG QIU³, JINGLI HUANG⁴, HUIPING JIANG¹, SHIYU HUANG¹, LILI LIU³
BIOCELL, Vol.45, No.3, pp. 785-796, 2021, DOI:10.32604/biocell.2021.014418
Abstract Taro (Colocasia esculenta (L.) Schott) is an important crop in Africa, Southeast Asia, and subtropics and is used as a food and medicine. The purple color pigmentation is an appealing character in taro. We sampled taro corms of the cultivar ‘Lipu Taro’ at four developmental stages, including LPYS1 (without purple pigment, 50 days of development (DOD)), LPYS2 (very few purple pigments, 75 DOD), LPYS3 (moderate purple pigments, 115 DOD) and LPYS4 (high purple pigments, 205 DOD). The purpose of our study was to identify the key genes underpinning the purple pigmentation in taro based on RNA-sequencing. Through RNA-Seq, 6453 differentially… More >

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