Home / Journals / BIOCELL / Vol.32, No.3, 2008
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  • Open Access

    ARTICLE

    A 66-kDa protein of bovine hypophyseal Pars tuberalis induces luteinizing hormone release from rat Pars distalis

    MARTHA LAFARQUE, LILIANA OLIVEROS
    BIOCELL, Vol.32, No.3, pp. 211-218, 2008, DOI:10.32604/biocell.2008.32.211
    Abstract In this study, evidence for a factor secreted by bovine hypophyseal pars tuberalis that stimulates luteinizing hormone (LH) release from rat pars distalis cells is shown. The secretion products of bovine pars tuberalis cells into the culture medium were assayed on dispersed rat pars distalis cells in 30 min incubations and superfusion experiments. The culture medium from pars tuberalis total cell populations, added at a dose of 6 μg per tube, induced the greater LH release from pars distalis cells, without effect on follicle stimulating hormone (FSH) release. After pars tuberalis cells separation on a discontinuos Percoll gradient, only the… More >

  • Open Access

    ARTICLE

    Isolation of epithelial cells, villi and crypts from small intestine of pigeons (Columba livia)

    OSCAR MAC DONAL1, JUAN G. CHEDIACK1,2,3, ENRIQUE CAVIEDES-VIDAL1,2,3
    BIOCELL, Vol.32, No.3, pp. 219-227, 2008, DOI:10.32604/biocell.2008.32.219
    Abstract The isolation of viable enterocytes, villi and crypts from the small intestine of a feral bird (Columba livia) is important for performing physiological experiments in ecologically relevant processes of membrane transport. The effectiveness of mechanical disruption, enzymatic digestion and chelating agents were compared. The objectives were to isolate enterocytes, villi and crypts from the small intestine of young pigeons; to evaluate the viability of the isolated intestinal epithelial cells isolated; and to verify the integrity of enterocytes by biochemical features. Enzymatic and mechanical methods yielded both elongated columnar and spherical cells. With the chelating method villi and crypts were obtained.… More >

  • Open Access

    ARTICLE

    Isolation and molecular characterization of a cax gene from Capsella bursa-pastoris

    JUAN LIN1, WEN ZHANG1, MINGZHU SHI1, XINGLONG WANG1, XIAOFEN SUN1, KEXUAN TANG1,2
    BIOCELL, Vol.32, No.3, pp. 229-235, 2008, DOI:10.32604/biocell.2008.32.229
    Abstract A new cation exchangers (CAXs) gene was cloned and characterized from Capsella bursapastoris by rapid amplification of cDNA ends (RACE). The full-length cDNA sequence of cax from C. bursa-pastoris (designated as Cbcax51) was 1754 bp containing a 1398 bp open reading frame encoding a polypeptide of 466 amino-acid residues with a calculated molecular mass of 50.5 kDa and an isoelectric point of 5.69. The predicted CbCAX51 contained an IMP dehydrogenase/GMP reductase domain, two Na+/Ca2+ exchanger protein domains. Comparative and bioinformatics analyses revealed that CbCAX51 showed extensive homology with CAX from other plant species. The expression analysis by different treatments indicated… More >

  • Open Access

    ARTICLE

    DNA mismatch repair gene methylation in gastric cancer in individuals from northern Brazil

    ELEONIDAS MOURA LIMA1,2, MARIANA FERREIRA LEAL3, MARÍLIA DE ARRUDA CARDOSO SMITH3, ROMMEL RODRÍGUEZ BURBANO4, PAULO PIMENTEL DE ASSUMPÇÃO5, MARIA JOSE BELLO6, JUAN ANTONIO REY6, FRANCINALDO FERREIRA DE LIMA7, CACILDA CASARTELLI2
    BIOCELL, Vol.32, No.3, pp. 237-243, 2008, DOI:10.32604/biocell.2008.32.237
    Abstract Gastric cancer is one of the most common malignancies. DNA methylation is implicated in DNA mismatch repair genes deficiency. In the present study, we evaluated the methylation status of MLH1, MSH2, MSH6 and PMS2 in 20 diffuse- and 26 intestinal-type gastric cancer samples and 20 normal gastric mucosal of gastric cancer patients from Northern Brazil. We found that none of the nonneoplastic samples showed methylation of any gene promoter and 50% of gastric cancer samples showed at least one methylated gene promoter. Methylation frequencies of MLH1, MSH2, MSH6 and PMS2 promoter were 21.74%, 17.39%, 0% and 28.26% respectively in gastric… More >

  • Open Access

    ARTICLE

    Agmatine pretreatment protects retinal ganglion cells (RGC-5 cell line) from oxidative stress in vitro

    IIZUKA YOKO1, SAMIN HONG2, CHAN YUN KIM2, SEUNG KAB KIM2, GONG JE SEONG2
    BIOCELL, Vol.32, No.3, pp. 245-250, 2008, DOI:10.32604/biocell.2008.32.245
    Abstract Agmatine, 2-(4-aminobutyl)guanidine, has been reported to have neuroprotective effects against various neuronal damages. In this study it was investigated whether agmatine pretreatment rescues the retinal ganglion cells from oxidative injury in vitro. After differentiation of transformed rat retinal ganglion cells (RGC-5 cell line) with staurosporine, agmatine (0.0 to 100.0 μM) pretreatment was performed for 2 hours. Subsequently, they were exposed to hydrogen peroxide (0.0 to 2.5 mM) as an oxidative stress. Cell viability was monitored for up to 48 hours with the lactate dehydrogenase (LDH) assay and apoptosis was examined by the terminal deoxynucleotide transferase-mediated terminal uridine deoxynucleotidyl transferase nick… More >

  • Open Access

    ARTICLE

    Sequential expressions of Notch1, Jagged2 and Math1 in molar tooth germ of mouse

    SILVIA S. BORKOSKY1,2, HITOSHI NAGATSUKA1, YORIHISA ORITA3, HIDETSUGU TSUJIGIWA4, JUNKO YOSHINOBU3, MEHMET GUNDUZ1, ANDREA P. RODRIGUEZ1, LILIANA R. MISSANA2, KAZUNORI NISHIZAKI3, NORIYUKI NAGAI1
    BIOCELL, Vol.32, No.3, pp. 251-257, 2008, DOI:10.32604/biocell.2008.32.251
    Abstract The Notch signaling pathway is an evolutionary conserved mechanism that plays an important role in cell-cell communication and cell fate in a wide range of tissues. The mammalian family of Notch receptors consists of 4 members: Notch1/2/3/4. The Notch ligand family consists of 5 members: Delta1/3/4 and Jagged1/2. Math1 encodes a murine basic helix-loop-helix (bHLH) transcription factor that acts as positive regulator of cell differentiation. Recently, links between Notch and Math1 pathways were demonstrated in various tissues. Expression of Notch1, Jagged2 and Math1 were analyzed in the mouse molar tooth germ during embryonic stage (E) 13 and E15 and during… More >

  • Open Access

    ARTICLE

    Heme metabolism enzymes are dynamically expressed during Xenopus embryonic development

    JIANLI SHI, WENYAN MEI, JING YANG
    BIOCELL, Vol.32, No.3, pp. 259-263, 2008, DOI:10.32604/biocell.2008.32.259
    Abstract As the key component of many hemoproteins (heme-containing proteins), heme is involved in a broad range of biological processes. Enzymes required for heme biosynthesis and degradation pathways are evolutionarily conserved. While heme metabolism has been studied extensively, the expression of heme metabolism enzymes during development has not been described. Here, we report that all heme biosynthases and two heme oxygenases, which initiate heme degradation, are dynamically expressed during Xenopus embryonic development. All heme synthases, with the exception of aminolevulinic acid synthase 2, are maternally expressed. At neurula stage, heme synthases are expressed in the developing neural tissue and in migrating… More >

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