Home / Journals / BIOCELL / Vol.32, No.2, 2008
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  • Open Access

    ARTICLE

    Cell proliferation of the ileum intestinal mucosa of diabetic rats treated with ascorbic acid

    JACQUELINE NELISIS ZANONI, RENATA VIRGINIA FERNANDES PEREIRA
    BIOCELL, Vol.32, No.2, pp. 163-168, 2008, DOI:10.32604/biocell.2008.32.163
    Abstract The objective of this work was to evaluate the effect of the ascorbic acid supplementation on the cellular proliferation on the ileum mucosa of diabetic rats. Fifteen 90-days rats were divided in the groups: control, diabetic and diabetic supplemented with ascorbic acid (DA). Two hours prior the sacrifice, they were injected with Vincristin. Semi-seriate histological cuts stained with HE were accomplished. About 2500 crypt cells from the intestinal mucosa were counted in order to obtain the metaphasic indexes. The height and depth of 30 villi and 30 crypts were measured for each animal, respectively. The metaphasic indexes showed no significant… More >

  • Open Access

    ARTICLE

    Rosette formation by macrophages with adhered T lymphocytes is precluded by inhibitors of antigen processing and presentation

    IVÓN TERESA NOVAK, HUMBERTO RAMÓN CABRAL
    BIOCELL, Vol.32, No.2, pp. 169-174, 2008, DOI:10.32604/biocell.2008.32.169
    Abstract We had previously found in autologous human leukocyte cultures, in which dead neutrophils phagocytosis by macrophages occur, macrophages and T CD4 lymphocytes perform a selective cell-cell interaction showing many figures of either one, two or several T- lymphocytes adhering to a central macrophage were seen. Considering that antigen presentation would be necessary for the formation of these immune synapses, we attempted to block rosette formation (i.e., the formation of macrophage associations with at least three lymphocytes) by interfering with both antigen processing and presentation. Culture samples of autologous leukocytes from 7 healthy donors were subjected to either brefeldin A, chloroquine… More >

  • Open Access

    ARTICLE

    Behavior of mesenchymal stem cells stained with 4’, 6-diamidino-2-phenylindole dihydrochloride (DAPI) in osteogenic and non osteogenic cultures

    N.M. OCARINO*, A. BOZZI**, R.D.O. PEREIRA*, N.M. BREYNER**, V.L. SILVA*, P. CASTANHEIRA**, A.M. GOES**, R. SERAKIDES*
    BIOCELL, Vol.32, No.2, pp. 175-183, 2008, DOI:10.32604/biocell.2008.32.175
    Abstract 4’, 6-diamidino-2-phenylindole dihydrochloride (DAPI) is a DNA dye widely used to mark and trace stem cells in therapy. We here studied the effect of DAPI staining on the behavior of mesenchymal stem cells cultured in either a control, non-osteogenic medium or in an osteogenic differentiation medium. In the control medium, the number of stem cells/field, as well as the number of fluorescent cells/field increased up to the sixth day in both control and DAPI-treated cultures. Afterwards, both the number of fluorescent cells and their fluorescence intensity decreased. Control cells were fusiform and with some long extensions that apparently linked them… More >

  • Open Access

    ARTICLE

    Morphologic and morphometric analysis of testis of Pseudis limellum (Cope, 1862) (Anura, Hylidae) during the reproductive cycle in the Pantanal, Brazil

    ADELINA FERREIRA1, MAHMOUD MEHANNA1,2, CYNTHIA P. A. PRADO3
    BIOCELL, Vol.32, No.2, pp. 185-194, 2008, DOI:10.32604/biocell.2008.32.185
    Abstract The spermatogenesis of Pseudis limellum, from the Southern Pantanal, Brazil, was studied from July 1995 to May 1996, through histological sections of the testis. The cells could be differentiated as: primary spermatogonia, large cells, generally with bilobed nucleus; secondary spermatogonia, smaller cells, with darker cytoplasm, chromatin of radial form; primary and secondary spermatocytes, differentiated according to the different stages of the nucleus during the successive cells divisions. Furthermore, we observed cells in process of morphologic differentiation: rounded spermatids much smaller, with nucleus containing chromatin in compacting process and cytoplasm reduction; elongated spermatids, generally parallel organized in well defined bundles, with… More >

  • Open Access

    ARTICLE

    Genotoxic effect of Physalis angulata L. (Solanaceae) extract on human lymphocytes treated in vitro

    RAQUEL ALVES DOS SANTOS1, TERESINHA ROSA CABRAL2, ISABEL ROSA CABRAL2, LUSÂNIA MARIA GREGGI ANTUNES3,4, CRISTIANE PONTES ANDRADE3, PLÍNIO CERQUEIRA DOS SANTOS CARDOSO1, MARCELO DE OLIVEIRA BAHIA2, CLAUDIA PESSOA5, JOSÉ LUIS MARTINS DO NASCIMENTO2, ROMMEL RODRÍGUEZ BURBANO2, CATARINA SATIE TAKAHASHI1,6
    BIOCELL, Vol.32, No.2, pp. 195-200, 2008, DOI:10.32604/biocell.2008.32.195
    Abstract Physalis angulata L (Solanaceae) is a medicinal plant from North of Brazil, whose different extracts and infusions are commonly used in the popular medicine for the treatment of malaria, asthma, hepatitis, dermatitis and rheumatism. However, the genotoxic effects of P. angulata on human cells is not well known. The main purpose of the present study was to evaluate the in vitro genotoxic effects of aqueous extract of P. angulata using the comet assay and the micronucleus assay in human lymphocytes provided from 6 healthy donors. Treatments with P. angulata extracts were performed in vitro in order to access the extent… More >

  • Open Access

    ARTICLE

    Short Communication: Agmatine inhibits hypoxia-induced TNF-α release from cultured retinal ganglion cells

    SAMIN HONG, KYOUNGSOO PARK, CHAN YUN KIM, GONG JE SEONG
    BIOCELL, Vol.32, No.2, pp. 201-205, 2008, DOI:10.32604/biocell.2008.32.201
    Abstract The effect of hypoxia on the release of tumor necrosis factor-α (TNF-α) in transformed rat retinal ganglion cells (RGCs) and the effect of agmatine on the hypoxia-induced production of TNF-α in RGCs were evaluated. RGCs were cultured under hypoxic conditions with 5% oxygen, with or without 100 μM agmatine. The expression levels of TNF-α and its receptor-1 (TNF-R1) were investigated by Western blot analysis. After 6 hours of hypoxia, we noted an increase in TNF-α production in RGCs. Agmatine significantly reduced TNF-α level after 12 hours of hypoxic treatment. The expression of TNF-R1 was not affected by the hypoxia or… More >

  • Open Access

    ARTICLE

    Brief Note : Chromosome comparison between populations of the collared peccary, Tayassu tajacu, raised in captivity

    PATRÍCIA CARVALHO DE SOUZA, ANDRÉ SALIM KHAYAT, IGOR CHAMON SELIGMANN, ROMMEL MARIO RODRÍGUEZ BURBANO
    BIOCELL, Vol.32, No.2, pp. 207-210, 2008, DOI:10.32604/biocell.2008.32.207
    Abstract The collared peccary (Tayassu tajacu) is widely distributed over the American continent, being found from the south of the USA to the north of Argentina. In Brazil, it is spread all over the country, being one of the potential species to be raised in captivity. Therefore, the cytogenetic techniques could be a potential tool for reproductive monitoring of animals raised in captivity, mainly when destined for commercial purposes. This study had the objective of determining the chromosome number of two populations raised in captivity and characterizing them by GTG banding. For this purpose, an analysis was made of mitotic metaphases… More >

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