Open Access

VIEWPOINT

DOMENICO MARIA CARRETTA^1,§, MARINA DI DOMENICO^2,§, ROBERTO LOVERO³, ROBERTO ARRIGONI⁴, ANGELIKA ELZBIETA WEGIERSKA⁵, MARIAROSARIA BOCCELLINO^2,*, ANDREA BALLINI^2,6,*, IOANNIS ALEXANDROS CHARITOS^7,#, LUIGI SANTACROCE^5,#
BIOCELL, Vol.46, No.8, pp. 1779-1788, 2022, DOI:10.32604/biocell.2022.020009
Abstract The existence of an inflammatory process in the heart muscle, related to a progressive worsening of myocardial function, different etiopathogenetic mechanisms concur and often overlap, thus making the diagnosis and the therapeutic approach complex. As the COVID-19 pandemic progresses, the effects of the disease on the organ systems and in particular on the cardiovascular system are becoming more and more profound. Cardiac involvement is a well-known event with a high percentage of findings in the heart’s magnetic field, even in asymptomatic areas. There are numerous uncertainties regarding their evolution, in the long and short term, due not only to a… More >

View

1773

Download

786

Open Access

REVIEW

WEIYU CHEN^1,2, MUHSIN H. YOUNIS³, ZHONGKUO ZHAO^1,*, WEIBO CAI^3,*
BIOCELL, Vol.46, No.8, pp. 1789-1801, 2022, DOI:10.32604/biocell.2022.018197
Abstract The knowledge of interactions among functional proteins helps researchers understand disease mechanisms and design potential strategies for treatment. As a general approach, the fluorescent and affinity tags were employed for exploring this field by labeling the Protein of Interest (POI). However, the autofluorescence and weak binding strength significantly reduce the accuracy and specificity of these tags. Conversely, HaloTag, a novel self-labeling enzyme (SLE) tag, could quickly form a covalent bond with its ligand, enabling fast and specific labeling of POI. These desirable features greatly increase the accuracy and specificity, making the HaloTag a valuable system for various applications ranging from… More >

View

1404

Download

669

Open Access

REVIEW

XIAOXIA WANG^1,#, ZHONGYUAN YAO^1,2,#, DI LIU¹, CHUNHONG YU^3,*, HUI LI^1,3,*
BIOCELL, Vol.46, No.8, pp. 1803-1806, 2022, DOI:10.32604/biocell.2022.019731
（This article belongs to this Special Issue: Reproductive Health and Embryonic Development)
Abstract Each stage of embryonic development, including normal gamete maturation, fertilization, zygotic genome activation, and cleavage, is crucial for human reproduction. Early embryo arrest is a common phenomenon. It is estimated that about 40%–70% of human embryos are arrested at early developmental stages. However, the exact mechanism remains largely uncertain. Embryos can be investigated in vitro by way of the development of in vitro fertilization/intracytoplasmic sperm injection. In addition to iatrogenic factors related to abnormal oocyte/embryo development, multiple gene mutations have been found to be involved in such phenotypes. Based on the knowledge of known etiological factors, several therapies are proposed… More >

View

1001

Download

415

Open Access

REVIEW

ADRIANA L. FERREIRA, GUSTAVO C. PARIS, ALINE DE A. AZEVEDO, ERIKA A. C. CORTEZ, SIMONE N. CARVALHO, LAIS DE CARVALHO, ALESSANDRA A. THOLE^*
BIOCELL, Vol.46, No.8, pp. 1807-1813, 2022, DOI:10.32604/biocell.2022.019363
（This article belongs to this Special Issue: Mesenchymal Stem Cells, Secretome and Biomaterials: Regenerative Medicine Application)
Abstract Mesenchymal stem cells (MSC) have pushed the field of stem cell-based therapies by inducing tissue regeneration, immunosuppression, and angiogenesis mainly through vesicles and soluble factors release (paracrine signaling). MSC-extracellular vesicles (MSC-EV) adaptable secretome and homing to injured sites allowed researchers to unlock a new era of cell-free based therapy. In parallel, nanoparticles (NP) have been explored in contributing to transport and drug delivery systems, giving drugs desired physical-chemical properties to exploit cell behavior. However, NPs can be quickly recognized by immune cells and cleared from circulation. In this viewpoint, we explore how combining both therapeutic strategies can improve efficacy and… More >

View

1529

Download

685

Open Access

VIEWPOINT

MASSIMO CONESE^1,*, AURELIO PORTINCASA²
BIOCELL, Vol.46, No.8, pp. 1815-1826, 2022, DOI:10.32604/biocell.2022.019448
（This article belongs to this Special Issue: Mesenchymal Stem Cells, Secretome and Biomaterials: Regenerative Medicine Application)
Abstract The treatment of nonhealing and chronic cutaneous wounds still needs a clinical advancement to be effective. Both mesenchymal stem cells (MSCs), obtained from different sources, and their secretome derived thereof (especially exosomes) can activate signaling pathways related to promotion of cell migration, vascularization, collagen deposition, and inflammatory response demonstrating prohealing, angiogenetic and anti-scarring capacities. On the other hand, biodegradable biomimetic scaffolds can facilitate endogenous cell attachment and proliferation as well as extracellular matrix production. In this Review, we revise the complex composites made by biomimetic scaffolds, mainly hydrogels, and MSC-derived exosomes constructed for cutaneous wound healing. Studies demonstrate that there… More >

View

1526

Download

719

Open Access

REVIEW

FRANCESCO PADUANO^1,2,*, EMANUELA ALTOMARE^2,3, BENEDETTA MARRELLI¹, VINCENZO DATTILO⁴, HAIZAL MOHD HUSSAINI⁵, PAUL ROY COOPER⁵, MARCO TATULLO⁶
BIOCELL, Vol.46, No.8, pp. 1827-1835, 2022, DOI:10.32604/biocell.2022.020462
Abstract Oral Cancer (OC) is one of the most recurrent cancers in the head and neck squamous cancer (SCCHN) category. Recently, the genome-wide association studies (GWAS) have gained growing interest in the scientific community. GWAS have identified several pathways involved in the interactions among general risk factors and genomic variants affecting SCCHN. This systematic overview aims to critically evaluate the latest data reported within the scientific literature. The aim was to investigate the impact of genetic aspects on SCCHN onset and prognosis, involving other clinical and systemic co-factors. PubMed, Google Scholar, and Cancer Genetics Web databases have been systematically investigated for… More >

View

1368

Download

624

Open Access

REVIEW

MARCO TATULLO^1,*, LUISA LIMONGELLI², ROSA MARIA MARANO³, ALESSANDRA VALLETTA⁴, ANGELA TEMPESTA², SANDRO RENGO⁴
BIOCELL, Vol.46, No.8, pp. 1837-1842, 2022, DOI:10.32604/biocell.2022.020570
Abstract The scientific community is continuously working to translate the novel biomedical techniques into effective medical treatments. CRISPR-Cas 9 system (Clustered Regularly Interspaced Short Palindromic Repeats-9), commonly known as the “molecular scissor”, represents a recently developed biotechnology able to improve the quality and the efficacy of traditional treatments, related to several human diseases, such as chronic diseases, neurodegenerative pathologies and, interestingly, oral diseases. Of course, dental medicine has notably increased the use of biotechnologies to ensure modern and conservative approaches: in this landscape, the use of CRISPR-Cas-9 system may speed and personalize the traditional therapies, ensuring a good predictability of clinical… More >

View

1427

Download

582

Open Access

REVIEW

PRANAB DUTTA^1,*, GOMATHY MUTHUKRISHNAN^2,*, SABARINATHAN KUTALINGAM GOPALASUBRAMAIAM², RAJAKUMAR DHARMARAJ², ANANTHI KARUPPAIAH³, KARTHIBA LOGANATHAN⁴, KALAISELVI PERIYASAMY⁵, M. ARUMUGAM PILLAI², GK UPAMANYA⁶, SARODEE BORUAH⁷, LIPA DEB¹, ARTI KUMARI¹, MADHUSMITA MAHANTA¹, PUNABATI HEISNAM⁸, AK MISHRA⁹
BIOCELL, Vol.46, No.8, pp. 1843-1859, 2022, DOI:10.32604/biocell.2022.019291
Abstract

Plant growth-promoting rhizobacteria (PGPR) are specialized bacterial communities inhabiting the root rhizosphere and the secretion of root exudates helps to, regulate the microbial dynamics and their interactions with the plants. These bacteria viz., Agrobacterium, Arthobacter, Azospirillum, Bacillus, Burkholderia, Flavobacterium, Pseudomonas, Rhizobium, etc., play important role in plant growth promotion. In addition, such symbiotic associations of PGPRs in the rhizospheric region also confer protection against several diseases caused by bacterial, fungal and viral pathogens. The biocontrol mechanism utilized by PGPR includes direct and indirect mechanisms direct PGPR mechanisms include the production of antibiotic, siderophore, and hydrolytic enzymes, competition for space and… More >

View

1071

Download

507

Open Access

VIEWPOINT

MASASHI KAWAMI^*, RYOKO YUMOTO, MIKIHISA TAKANO
BIOCELL, Vol.46, No.8, pp. 1861-1865, 2022, DOI:10.32604/biocell.2022.019667
Abstract A number of drugs induce pulmonary injury and subsequently lead to serious lung diseases such as pulmonary fibrosis as the adverse drug reactions. However, an effective preventive approach against drug-induced pulmonary fibrosis has not been established due to poor understanding of common preventive targets in a variety of drugs showing pulmonary toxicity. Epithelial-mesenchymal transition (EMT), a cellular phenotypic change of the epithelial to mesenchymal state, contributes to the development of pulmonary fibrosis through the conversion of damaged alveolar epithelium into myofibroblasts. As several drugs with pulmonary toxicity have been reported to induce EMT, EMT serves as a bridge between the… More >

View

1421

Download

600

Open Access

VIEWPOINT

ANA M. CÁRDENAS^1,*, LUCIANA I. GALLO², FERNANDO D. MARENGO^2,*
BIOCELL, Vol.46, No.8, pp. 1867-1873, 2022, DOI:10.32604/biocell.2022.019086
Abstract The cortical actin network is a mesh of filaments distributed beneath the plasmalemma that dynamically reacts in response to stimuli. This dynamic network of cortical filaments, together with motor myosin partners, adjusts the plasmalemma tension, organizes membrane protein microdomains, remodels the cell surface and drives vesicle motion in order to fine-tune exocytosis, endocytosis and recycling of secretory vesicles. In this review, we discuss how these mechanisms work in secretory cells. More >

View

1443

Download

695

Open Access

VIEWPOINT

VITTORIO PICCHIO¹, FRANCESCA PAGANO², ISOTTA CHIMENTI^1,3,*
BIOCELL, Vol.46, No.8, pp. 1875-1877, 2022, DOI:10.32604/biocell.2022.020181
Abstract Cardiac stromal cells have faced through the years a significant evolution in their definitions concerning their phenotypes, markers, and functions. They are surging to key roles in physiopathology, becoming important targets to be exploited for cardiac repair. In this perspective, we briefly discuss their role in novel therapeutic strategies for enhancing cardiac repair and regeneration. More >

View

1575

Download

624

Open Access

VIEWPOINT

CARLO DAL LIN^1,*, SABINO ILICETO¹, FRANCESCO TONA¹, GIUSEPPE VITIELLO²
BIOCELL, Vol.46, No.8, pp. 1879-1884, 2022, DOI:10.32604/biocell.2022.019169
Abstract In this view point paper, we briefly summarize some of the clinical, biochemical and biophysical results obtained in our research on Relaxation Response. We also qualitatively describe the theoretical biophysical model that could link them. Our work points to a unified view of the human biological system activity, joining the dynamics ruling the interactions and correlations of the microscopic components to the knowledge of their specific individual properties in the effort of going beyond a purely atomistic approach. More >

View

1254

Download

510

Cited by

1

Open Access

VIEWPOINT

FUAD GANDHI TORIZAL^1,2,*, FRANSISCUS FIANO ANTHONY KERANS³, ANNISA KHUMAIRA¹
BIOCELL, Vol.46, No.8, pp. 1885-1891, 2022, DOI:10.32604/biocell.2022.019591
（This article belongs to this Special Issue: Mesenchymal Stem Cells, Secretome and Biomaterials: Regenerative Medicine Application)
Abstract The potential of mesenchymal stem cells (MSCs) in regenerative medicine has been largely known due to their capability to induce tissue regeneration in vivo with minimum inflammation during implantation. This adult stem cell type exhibit unique features of tissue repair mechanism and immune modulation mediated by their secreted factors, called secretome. Recently, the utilization of secretome as a therapeutic agent provided new insight into cell-free therapy. Nevertheless, a sufficient amount of secretome is necessary to realize their applications for translational medicine which required a proper biomanufacturing process. Several factors related to their production need to be considered to produce a… More >

View

1713

Download

714

Open Access

ARTICLE

MAGDALENA RUDZIŃSKA-RADECKA^*
BIOCELL, Vol.46, No.8, pp. 1893-1901, 2022, DOI:10.32604/biocell.2022.019724
（This article belongs to this Special Issue: Recent Advancement in Cancer Molecular Signaling)
Abstract Clear cell renal cell carcinoma (KIRC) is the most common and aggressive malignancy subtype of renal neoplasm that arises from proximal convoluted tubules. It is characterized by poor clinical outcomes and high mortality of patients due to the lack of specific biomarkers for varying stages of the disease and no effective treatment. Proteases are associated with the development of several malignant tumors in humans by their ability to degrade extracellular matrices, facilitating metastasis. Herein, differentially expressed genes in KIRC cases compared to healthy kidneys were screened out from the Gene Expression Profiling Interactive Analysis (GEPIA) database. This data was applied… More >

View

1513

Download

746

Open Access

ARTICLE

PANKE CHEN^*, SHUAI MA
BIOCELL, Vol.46, No.8, pp. 1903-1909, 2022, DOI:10.32604/biocell.2022.018651
（This article belongs to this Special Issue: Cell-Based Regenerative Therapies)
Abstract This work aimed to investigate the effects of calcitonin gene-related peptide (CGRP)-modified mesenchymal stem cells (MSCs) on vascular stenosis in carotid balloon-injured rats. The CGRP gene labeled with EGFP was transfected into bone marrow MSCs, and CGRP protein expression in MSCs was confirmed by immunofluorescence assays. A rat carotid balloon injury model was established using a surgical method. CGRP-modified MSCs were orthotopically transplanted into the injured area of the rats. At 28 days after cell transplantation, EGFP and CD31 expression was detected by immunofluorescence staining. Hematoxylin-eosin (H&E) staining was used to detect the intima/media area of the injured carotid artery… More >

View

877

Download

493

Open Access

ARTICLE

LIANHUAN MA¹, SHOUPENG LIU², XIAOWEN ZHEN¹, WEIWEI QIAO¹, LINA MA¹, XIAOMIN ZHANG^3,*
BIOCELL, Vol.46, No.8, pp. 1911-1916, 2022, DOI:10.32604/biocell.2022.019277
（This article belongs to this Special Issue: Single Cell Technologies and Molecular Mechanisms of Diseases)
Abstract This study was designed to investigate the protective effects of Astaxanthin (AST) in rats with diabetes mellitus (DM) induced by streptozotocin. SD rats were divided into control group (n = 5, only received normal saline), DM group (n = 8) and AST + DM group (n = 8; AST: 50 mg/kg/day). DM rats were induced by intraperitoneal injection of streptozocin (STZ, 65 mg/kg). Blood glucose level and body weight were determined at weeks 0, 2, 4, 6 and 8, respectively. At week 8, kidney function was determined, together with expression of P53 and dynamin-related protein-1 (Drp1) by Western blot analysis… More >

View

1512

Download

551

Open Access

ARTICLE

ZHIYONG ZHANG^1,#, YAN PAN^2,#, YAN ZHAO¹, MUDAN REN¹, YARUI LI¹, YUN FENG¹, GUIFANG LU^1,*, SHUIXIANG HE^1,*
BIOCELL, Vol.46, No.8, pp. 1917-1924, 2022, DOI:10.32604/biocell.2022.018847
Abstract Colorectal cancer (CRC) is a heterogeneous cancer, and many risk factors for colorectal cancer have been established. For CRC metastasis, tumor cell migration, adhesion as well as invasion are important processes. WiskottAldrich syndrome protein family member 3 (WASF3) is necessary for metastasis of various types of cancers. However, its role in CRC progression has not been fully elucidated. This study examined the in vitro functional roles of WASF3 in the CRC and explored the underlying molecular mechanisms. We used siRNA-WASF3 to gene silence WASF3 in colon cancer cell (HCT116) in vitro. The effects of WASF3 silencing on HCT116 cell apoptosis,… More >

View

1501

Download

616

Open Access

ARTICLE

BUWEN CAO^1,*, JIAWEI LUO^2,*, SAINAN XIAO^1,2, XIANGJUN ZHOU¹
BIOCELL, Vol.46, No.8, pp. 1925-1933, 2022, DOI:10.32604/biocell.2022.019613
（This article belongs to this Special Issue: Computational Models in Non-Coding RNA and Human Disease)
Abstract The association between miRNA and disease has attracted more and more attention. Until now, existing methods for identifying miRNA related disease mainly rely on top-ranked association model, which may not provide a full landscape of association between miRNA and disease. Hence there is strong need of new computational method to identify the associations from miRNA group view. In this paper, we proposed a framework, MDA-TOEPGA, to identify miRNAdisease association based on two-objective evolutionary programming genetic algorithm, which identifies latent miRNAdisease associations from the view of functional module. To understand the miRNA functional module in diseases, the case study is presented.… More >

View

1454

Download

621

Open Access

ARTICLE

MENGMENG WU^1,#, PENG GUO^1,#, YISU SHI^1,#, DANYAN ZHENG¹, QIAONAN ZHANG¹, XIN JIN¹, YUHUA WANG¹, QIANG LIN², RONGJUN FANG^1,*, WEIGUO ZHAO^1,*
BIOCELL, Vol.46, No.8, pp. 1935-1945, 2022, DOI:10.32604/biocell.2022.019223
（This article belongs to this Special Issue: Decoding Gene (including circRNA, lincRNA miRNA and mRNA) Expression)
Abstract A variety of plants were colchicine treated to double their chromosome number. Chromosomes are genetic carriers that determine the individual traits of organisms. The doubling of chromosomes will lead to modifications in plant morphology, physiology and genetics. To determine the response of mulberry trees induced by colchicine, using mulberry variety Yu-711 leaves as research materials, two small RNA libraries (control and experimental groups) were constructed. It was found that 45 known miRNA genes and 78 predicted novel miRNA genes in the sequence results. A comparison of data between the control group and the experimental group revealed 37 differentially expressed miRNA… More >

View

1410

Download

633

Open Access

ARTICLE

XIN LI¹, JIANMIN PAN¹, FAISAL ISLAM², JUANJUAN LI¹, ZHUONI HOU¹, ZONGQI YANG¹, LING XU^1,*
BIOCELL, Vol.46, No.8, pp. 1947-1958, 2022, DOI:10.32604/biocell.2022.019806
（This article belongs to this Special Issue: Physiology and Molecular Biology of Plant Stress Tolerance)
Abstract NAC (NAM, ATAF, CUC) is a class of transcription factors involved in plant growth regulation, abiotic stress responses, morphogenesis and metabolism. Salvia miltiorrhiza is an important Chinese medicinal herb, but the characterization of NAC genes in this species is limited. In this study, based on the Salvia miltiorrhiza genomic databases, 82 NAC transcription factors were identified, which were divided into 14 groups. Meanwhile, phylogenetic analysis, gene structure, chromosomal localization and potential role of SmNACs in abiotic stress conditions were also studied. The results revealed that some SmNACs had different structures than others, which advised that these genes may have multiple/distinct… More >

View

1736

Download

824

Open Access

ARTICLE

MING-AO CAO¹, FEI ZHANG², ELSAYED FATHI ABD_ALLAH³, QIANGSHENG WU^1,*
BIOCELL, Vol.46, No.8, pp. 1959-1966, 2022, DOI:10.32604/biocell.2022.020391
（This article belongs to this Special Issue: Mycorrhizal Fungal Roles in Stress Tolerance of Plants)
Abstract A potted experiment was carried out to study the effect of an arbuscular mycorrhizal fungus (Diversispora versiformis) and arbuscular mycorrhizal like fungus (Piriformospora indica) on antioxidant enzyme defense system of Satsuma orange (Citrus sinensis cv. Oita 4) grafted on Poncirus trifoliata under favourable temperature (25°C) and cold temperature (0°C) for 12 h. Such short-term treatment of cold temperature did not cause any significant change in root fungal colonization and spore density in soil. Under cold stress, D. versiformis inoculation did not change the activity of superoxide dismutase (SOD), catalase (CAT), and peroxidase (POD) in leaves and roots, whereas P. indica… More >

View

1767

Download

766

Open Access

ARTICLE

YUNHUI ZHOU, YAJIE LIU, YUDAN WANG, CHUNXUE YANG^*
BIOCELL, Vol.46, No.8, pp. 1967-1978, 2022, DOI:10.32604/biocell.2022.019304
（This article belongs to this Special Issue: Mycorrhizal Fungal Roles in Stress Tolerance of Plants)
Abstract To investigate the effects of arbuscular mycorrhizal (AM) fungi on the growth and saline–alkaline tolerance of Potentilla anserina L., the seedlings were inoculated with Claroideoglomus etunicatum (W.N. Becker & Gerd.) C. Walker & A. Schüßler in pot cultivation. After 90 days of culture, saline–alkaline stress was induced with NaCl and NaHCO₃ solution according to the main salt components in saline–alkaline soils. Based on the physiological response of P. anserina to the stress in the preliminary experiment, the solution concentrations of 0 mmol/L, 75 mmol/L, 150 mmol/L, 225 mmol/L and 300 mmol/L were treated with stress for 10 days, respectively. The… More >

View

1443

Download

742

Open Access

ARTICLE

MOHAMED SALEM^1,2, MOHAMMAD EL-METWALLY³, WESAMELDIN SABER^4,*, SALLY NEGM^5,6, ATTALLA EL-KOTT^7.8, YASSER MAZROUA^9,10, ABEER MAKHLOUF¹¹, MAHMOUD MOUSTAFA^7,12
BIOCELL, Vol.46, No.8, pp. 1979-1988, 2022, DOI:10.32604/biocell.2022.019301
Abstract Profound inspection of the life forms on the earth teaches how to be the complexity of interrelationships among the various systems. Because of the emergence of novel viruses all the time and the inadequate of vaccines and antivirals, viral contagions are amongst the most causative diseases affecting people worldwide. Fungi exemplify a massive source of bioactive molecules as, many fungal secondary metabolities like Oxoglyantrypine, Carneic acid F, Scedapin C, Asteltoxin E, Phomanolide, Norquinadoline A and Quinadoline B have antiviral activity. This review deals with how secondary metabolites of fungi can help in the war against viruses in general and especially… More >

View

1504

Download

644

Open Access

ARTICLE

WEI LIANG^1,2,*, QIANG LUO^1,2,#, ZONGWEI ZHANG^1,2,#, KEJU YANG^1,2,3, ANKANG YANG^1,2, QINGJIA CHI⁴, HUAN HU⁵
BIOCELL, Vol.46, No.8, pp. 1989-2002, 2022, DOI:10.32604/biocell.2022.019300
（This article belongs to this Special Issue: Decoding Gene (including circRNA, lincRNA miRNA and mRNA) Expression)
Abstract Diabetic nephropathy (DN) is a common microvascular complication that easily leads to end-stage renal disease. It is important to explore the key biomarkers and molecular mechanisms relevant to diabetic nephropathy (DN). We used highthroughput RNA sequencing to obtain the genes related to DN glomerular tissues and healthy glomerular tissues of mice. Then we used LIMMA to analyze differentially expressed genes (DEGs) between DN and non-diabetic glomerular samples. And we performed KEGG, gene ontology functional (GO) enrichment, and gene set enrichment analysis to reveal the signaling pathway of the disease. The CIBERSORT algorithm based on support vector machine was used to… More >

View

1731

Download

788