Home / Journals / BIOCELL / Vol.47, No.12, 2023
Special lssues
Table of Content
  • Open AccessOpen Access

    REVIEW

    Realizing the potential of exploiting human IPSCs and their derivatives in research of Down syndrome

    YAFEI WANG1,2,#, JIELEI NI1,#, YUHAN LIU2, DINGYING LIAO3, QIANWEN ZHOU1, XIAOYANG JI2, GANG NIU2, YANXIANG NI1,*
    BIOCELL, Vol.47, No.12, pp. 2567-2578, 2023, DOI:10.32604/biocell.2023.043781
    (This article belongs to this Special Issue: Perspectives on Stem Cells and Regenerative Medicine)
    Abstract Down syndrome (DS) is a genetic condition characterized by intellectual disability, delayed brain development, and early onset Alzheimer’s disease. The use of primary neural cells and tissues is important for understanding this disease, but there are ethical and practical issues, including availability from patients and experimental manipulability. Moreover, there are significant genetic and physiological differences between animal models and humans, which limits the translation of the findings in animal studies to humans. Advancements in induced pluripotent stem cells (iPSC) technology have revolutionized DS research by providing a valuable tool for studying the cellular and molecular pathologies associated with DS. Induced… More >

  • Open AccessOpen Access

    REVIEW

    A perspective on molecular and cellular biology-based developmental toxicology biomarkers

    SUKHENDU DEY1,#, SANDIPAN PAL2,#, APURBA RATAN GHOSH1, PALAS SAMANTA3,*
    BIOCELL, Vol.47, No.12, pp. 2579-2590, 2023, DOI:10.32604/biocell.2023.031114
    Abstract The process of development is intricate and couple-dependent phenomenon. Accordingly, the study of molecular and cellular biology-based developmental toxicology biomarkers increasingly is becoming an important part of risk assessment and management of chemicals for detection of health outcomes and/or biological endpoint like cytotoxicity, cell death, etc. Since, the evolution of developmental toxicology field a number of tools/markers have been developed or addressed to deal with developmental outcomes, which can ultimately be used for the development of adverse outcome pathways (AOPs) of developmental toxicants. As a result, this paper provides an overview of the current state of developmental toxicology biomarkers and… More >

  • Open AccessOpen Access

    REVIEW

    Reservoir of human immunodeficiency virus in the brain: New insights into the role of T cells

    YINGDONG ZHANG1,2, MING CHU1,2,*, HONGZHOU LU3,*
    BIOCELL, Vol.47, No.12, pp. 2591-2595, 2023, DOI:10.32604/biocell.2023.030331
    (This article belongs to this Special Issue: Neuroimmune Interactions at the Crossroads of Health and Disease)
    Abstract Human immunodeficiency virus (HIV) infection of the central nervous system (CNS) has attracted significant attention because it contributes to severe complications of acquired immunodeficiency syndrome (AIDS) and seriously impairs the life quality of infected patients. In this review, we briefly describe the latent infection of HIV in CNS and focus on the role of the important immune cells, such as T cells, in the formation and maintenance of the HIV reservoir in CNS. This review explores the mechanisms by which T cells enter CNS and establish latent infection of HIV in the CNS. In conclusion, we summarize the role of… More >

    Graphic Abstract

    Reservoir of human immunodeficiency virus in the brain: New insights into the role of T cells

  • Open AccessOpen Access

    REVIEW

    Exosomes in viral infection: Effects for pathogenesis and treatment strategies

    FATEMEH HEIDARI1,2, REIHANEH SEYEDEBRAHIMI1,2, PIAO YANG3, MOHSEN ESLAMI FARSANI1,2, SHIMA ABABZADEH2,4, NASER KALHOR5, HAMED MANOOCHEHRI6, MOHSEN SHEYKHHASAN7,*, MARYAM AZIMZADEH8,9,*
    BIOCELL, Vol.47, No.12, pp. 2597-2608, 2023, DOI:10.32604/biocell.2023.043351
    (This article belongs to this Special Issue: Perspectives on Stem Cells and Regenerative Medicine)
    Abstract Exosomes are small vesicles that carry molecules from one cell to another. They have many features that make them interesting for research, such as their stability, low immunogenicity, size of the nanoscale, toxicity, and selective delivery. Exosomes can also interact with viruses in diverse ways. Emerging research highlights the significant role of exosomes in viral infections, particularly in the context of diseases like COVID-19, HIV, HBV and HCV. Understanding the intricate interplay between exosomes and the human immune system holds great promise for the development of effective antiviral therapies. An important aspect is gaining clarity on how exosomes influence the… More >

  • Open AccessOpen Access

    ARTICLE

    Evaluation of combined detection of nuclear factor erythroid 2-related factor 2 and glutathione peroxidase 4 in primary hepatic carcinoma and preliminary exploration of pathogenesis

    JIE DUAN, AIDONG GU*, WEI CHEN, CHANGHAO CHEN, FANGNAN SONG, FAXI CHEN, FANGFANG JIANG, HUIWEN XING
    BIOCELL, Vol.47, No.12, pp. 2609-2615, 2023, DOI:10.32604/biocell.2023.042472
    (This article belongs to this Special Issue: Novel Biomarkers in Diseases for Detection, Diagnosis, and Prognosis)
    Abstract Objective: This study aims to analyze the clinical significance and mechanism of nuclear factor erythroid 2-related factor 2 (NRF2) and glutathione peroxidase 4 (GPX4) in primary hepatic carcinoma (PHC). Methods: The expression of NRF2 and GPX4 in peripheral blood of patients with PHC was determined to analyze the diagnostic value of the two combined for PHC. The prognostic significance of NRF2 and GPX4 was evaluated by 3-year follow-up. Human liver epithelial cells THLE-2 and human hepatocellular carcinoma cells HepG2 were purchased, and the expression of NRF2 and GPX4 in the cells was determined. NRF2 and GPX4 aberrant expression vectors were… More >

    Graphic Abstract

    Evaluation of combined detection of nuclear factor erythroid 2-related factor 2 and glutathione peroxidase 4 in primary hepatic carcinoma and preliminary exploration of pathogenesis

  • Open AccessOpen Access

    ARTICLE

    UCHL5 inhibits U251 glioma cell proliferation and tumor growth via stabilizing and deubiquitinating PTEN

    YUE XIAO1,2,#, WENJING MA2,#, XINYI CHEN2, WEIWEI HU3, QIANQIAN DI2, XIBAO ZHAO2, GUODONG HUANG1, WEILIN CHEN1,2,*
    BIOCELL, Vol.47, No.12, pp. 2617-2625, 2023, DOI:10.32604/biocell.2023.042476
    (This article belongs to this Special Issue: Epigenetic Regulation of Cancer)
    Abstract Background: Glioma is the most common primary brain tumor. Exploration of new tumorigenesis mechanism of glioma is critical to determine more effective treatment targets as well as to develop effective prognosis methods that can enhance the treatment efficacy. We previously demonstrated that the deubiquitinase biquitin carboxyl-terminal hydrolase L5 (UCHL5) was downregulated in human glioma. However, the effect and mechanism of UCHL5 on the proliferation of glioma cells remains unknown. Methods: Transfection of siRNA was used to knockdown the expression of UCHL5 in U251 cells. The 3-[4,5-dimethylthiazol-2-yl]-2,5 diphenyl tetrazolium bromide (MTT) assay, Edu assay, and colony formation assay were employed to… More >

    Graphic Abstract

    UCHL5 inhibits U251 glioma cell proliferation and tumor growth via stabilizing and deubiquitinating PTEN

  • Open AccessOpen Access

    ARTICLE

    Bone marrow mesenchymal stem cell-induced autophagy ameliorates TNBS-induced experimental colitis by downregulating the NLRP3 inflammasome

    JINJIN FU1,#, XIAOYUE FENG2,#, JUAN WEI2, XIANG GENG1, YU GONG1, FENGDONG LI1, SHAOHUA ZHUANG1, JIN HUANG1, FANGYU WANG2,*
    BIOCELL, Vol.47, No.12, pp. 2627-2639, 2023, DOI:10.32604/biocell.2023.042586
    Abstract Background: This study aimed to elucidate the potential mechanisms through which bone marrow-derived mesenchymal stem cells (BM-MSCs) may be effective in alleviating experimental colitis induced by treatment with 2,4,6-trinitrobenzene-sulfonate acid (TNBS), specifically through autophagy modulation. Methods: BM-MSCs were collected from BALB/c mice for subsequent experiments. The study employed cell counting kits (CCK-8) to investigate the impact of the MSC-conditioned medium (M medium) on the proliferation of RAW264.7 macrophages. The GFP-mRFP-LC3 adenovirus was transfected into RAW264.7 to detect autophagic flux. The gene expression of cytokines was assessed through quantitative reverse transcription polymerase chain reaction (qRT-PCR). Western blot analysis was employed to… More >

  • Open AccessOpen Access

    ARTICLE

    Regulation of RNA methylation and immune infiltration patterns by m5C regulators in head and neck squamous cell carcinoma

    SHIDA HOU1,#, TIANJUN LAN2,#, YAOCHENG YANG3,#, PEISHENG LIANG1, XIN LIU4,5, JUNJIE WANG6, ZHIFENG CHEN7, RONGSHENG ZENG1,*, ZIJING HUANG8,*
    BIOCELL, Vol.47, No.12, pp. 2641-2660, 2023, DOI:10.32604/biocell.2023.043291
    (This article belongs to this Special Issue: Frontiers in cancer: tumor microenvironment)
    Abstract Background: 5-Methylcytosine (m5C) methylation contributes to the development and progression of various malignant tumors. This study aimed to explore the potential role of m5C methylation regulators (m5CMRs) in head and neck squamous cell carcinoma (HNSCC). Methods: The transcription data of HNSCC samples were obtained from The Cancer Genome Atlas (TCGA) and the Gene Expression Omnibus (GEO) databases. Subsequently, the m5C patterns in HNSCC were evaluated based on 14 m5CMRs. Then, the m5Cscore was developed to quantify m5C patterns by using principal component analysis (PCA) algorithms. Two single-cell RNA sequencing datasets and various methods were employed to assess the prognostic value… More >

  • Open AccessOpen Access

    ARTICLE

    Effects of platelets on characteristics of lymphocytes cultured in vitro and optimization of adoptive immunotherapy

    CONGLIANG CHEN#, XIAOHONG LENG#, YU ZHANG, JUNMEI HU, DAPENG WEI, PEIPEI WANG*, XIA WANG*
    BIOCELL, Vol.47, No.12, pp. 2661-2669, 2023, DOI:10.32604/biocell.2023.043084
    (This article belongs to this Special Issue: Extracellular Vesicles and Cancer)
    Abstract Background: T lymphocytes, the main participants of cellular immunity, can express a variety of surface molecules and form different lymphocyte subsets under the induction of different factors to play the functions of immune regulation and immune killing. Studies have shown that platelets play a crucial role in maintaining the stable differentiation of lymphocytes and the balance in immunomodulation. Therefore, it is necessary to study the effect of platelets on lymphocytes in vitro to better understand the role of platelets in the immune system and broaden the application of adoptive immunotherapy. Methods: Cell counting and microscopic observation were used to detect… More >

  • Open AccessOpen Access

    ARTICLE

    LIM1863 is useful to explore collective cancer cell migration, and the group of heterogeneous cells undergoing collective migration behaves like a supracellular unit

    JINSONG WU1,2, ZHENG ZHI1, WENZHONG XU1, DIANCGENG LI1, QIUBO LI1, YAN HAN1, JIANMING HE1,3,*, XI LIANG1,*
    BIOCELL, Vol.47, No.12, pp. 2671-2680, 2023, DOI:10.32604/biocell.2023.043494
    Abstract Introduction: Collective cancer cell migration (CCCM) and epithelial-to-mesenchymal transition (EMT) play key roles in metastasis. This study reports that the colorectal carcinoma cell line LIM1863 is useful for the study of CCCM and EMT. Methods: Hematoxylin and eosin staining, scanning electron microscopy, transmission electron microscopy, and western blot analysis were performed. Results: LIM1863 automatically grew as spheroids in suspension and had important typical epithelial properties, including several layers of cells arranged around a central lumen, apical-basal polarity, and types of cell-cell junctions. Treatment with a combination of both TGF beta 1 and TNF alpha induced definite and distinct EMT, a… More >

  • Open AccessOpen Access

    ARTICLE

    Identification of an immune classifier for predicting the prognosis and therapeutic response in triple-negative breast cancer

    KUAILU LIN1,2, QIANYU GU2, XIXI LAI2,3,*
    BIOCELL, Vol.47, No.12, pp. 2681-2696, 2023, DOI:10.32604/biocell.2023.043298
    (This article belongs to this Special Issue: Frontiers in cancer: tumor microenvironment)
    Abstract Objectives: Triple-negative breast cancer (TNBC) poses a significant challenge due to the lack of reliable prognostic gene signatures and an understanding of its immune behavior. Methods: We analyzed clinical information and mRNA expression data from 162 TNBC patients in TCGA-BRCA and 320 patients in METABRIC-BRCA. Utilizing weighted gene coexpression network analysis, we pinpointed 34 TNBC immune genes linked to survival. The least absolute shrinkage and selection operator Cox regression method identified key TNBC immune candidates for prognosis prediction. We calculated chemotherapy sensitivity scores using the “pRRophetic” package in R software and assessed immunotherapy response using the Tumor Immune Dysfunction and… More >

  • Open AccessOpen Access

    ARTICLE

    Knockdown Annexin A8 inhibits the proliferation and invasion of cervical cancer cells

    WEILING ZHANG1,2, YONG LI2, CAN ZHANG2, QING HAN2, YU ZHANG2, AIQIN HE2, WEIPEI ZHU1,*
    BIOCELL, Vol.47, No.12, pp. 2697-2708, 2023, DOI:10.32604/biocell.2023.044314
    Abstract Background: This study aimed to explore the expression, function, and molecular mechanism of ANXA8, the gene for annexin 8, in cervical cancer. Methods: The gene expression of the ANX family members in cervical cancer tissues was classified via The Cancer Genome Atlas (TCGA) database. The expression of ANXA8 in paracancerous tissues, cervical cancer tissues, and cell lines was identified by fluorescence quantitative polymerase chain reaction (PCR) and immunohistochemistry. The effects of ANXA8 knockdown on the cellular growth and cell invasion of cervical cancer were examined by MTT, clone-formation assay, scratch test, and Transwell assay. The effect of ANXA8 knockdown on… More >

  • Open AccessOpen Access

    ARTICLE

    Suppression of cell pyroptosis by omeprazole through PDE4-mediated autophagy in gastric epithelial cells

    LIPING YE1,2,3,#, HUIYAN SUN4,#, XINHUA LIANG2,#, WENXU PAN2, LI XIANG2,3, WENJUN DU2, LANLAN GENG2, WANFU XU2,3,*, SITANG GONG1,2,3,*
    BIOCELL, Vol.47, No.12, pp. 2709-2719, 2023, DOI:10.32604/biocell.2023.044295
    Abstract Introduction: Helicobacter pylori is a risk factor for the development of peptic ulcers with autophagy dysfunction. Omeprazole was widely known as the first-line regimen for H. pylori-associated gastritis. Objectives: The objective of this work was to assess the role of omeprazole on cell pyroptosis and autophagy. Methods: The clinical samples were collected. Quantitative polymerase chain reaction, western blotting, enzyme linked immunosorbent assay, and immunofluorescence (IF) analysis were conducted to reveal the mechanism of omeprazole on cell pyroptosis and autophagy. Results: The results revealed that omeprazole could decrease cell pyroptosis, which was attributed to the downregulation of cleaved caspase-1 expression, resulting… More >

  • Open AccessOpen Access

    ARTICLE

    Long non-coding RNA DPP10-AS1 represses the proliferation and invasiveness of glioblastoma by regulating miR-24-3p/CHD5 signaling pathway

    JIWEI SUN1,2,#, LIANG XU1,#, YESEN ZHANG2, HAORAN LI1, JIE FENG2, XUEFENG LU2, JUN DONG1,*
    BIOCELL, Vol.47, No.12, pp. 2721-2733, 2023, DOI:10.32604/biocell.2023.043869
    Abstract Objective: This investigation aimed to unveil new prospective diagnosis-related biomarkers together with treatment targets against glioblastoma. Methods: The expression levels of long non-coding RNA (lncRNA) DPP10-AS1 were assessed using real-time quantitative polymerase chain reaction (RT-qPCR) within both the patient tissue specimens and glioblastoma cell lines. The relationship between lncRNA DPP10-AS1 expression in glioblastoma and patient prognosis was investigated. Cell Counting Kit-8 (CCK-8), transwell, and clonogenic experiments were utilized to assess tumor cells’ proliferation, invasiveness, and migratory potentials after lncRNA DPP10-AS1 expression was up or down-regulated. Using an online bioinformatics prediction tool, the intracellular localization of lncRNA DPP10-AS1 and its target… More >

  • Open AccessOpen Access

    ARTICLE

    Bioinformatic analysis and in vivo validation of angiogenesis related genes in inflammatory bowel disease

    ZEPENG DONG, CHENYE ZHAO, SHIBO HU, KUI YANG, JUNHUI YU*, XUEJUN SUN, JIANBAO ZHENG*
    BIOCELL, Vol.47, No.12, pp. 2735-2745, 2023, DOI:10.32604/biocell.2023.043422
    (This article belongs to this Special Issue: Bioinformatics Study of Diseases)
    Abstract Objectives: Angiogenesis plays a significant role in the occurrence and development of inflammatory bowel disease (IBD). The aim of this study is to explore potential angiogenesis related genes (ARGs) in IBD through bioinformatics analysis and in vivo experiments. Methods: GSE57945, GSE87466, and GSE36807 were obtained from the Gene Expression Omnibus database. GSE57945 was used as the training set, while GSE87466 and GSE36807 were used as the validation set. The key ARGs associated with IBD were identified using the least absolute shrinkage and selection operator (LASSO) and random forest methods. These identified ARGs were then utilized to construct a diagnostic model… More >

Per Page:

Share Link