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LIM1863 is useful to explore collective cancer cell migration, and the group of heterogeneous cells undergoing collective migration behaves like a supracellular unit

JINSONG WU1,2, ZHENG ZHI1, WENZHONG XU1, DIANCGENG LI1, QIUBO LI1, YAN HAN1, JIANMING HE1,3,*, XI LIANG1,*

1 Department of Radiotherapy, Hebei Province Hospital of Chinese Medicine, Hebei University of Chinese Medicine, Shijiazhuang, China
2 Department of General Surgery, Chongqing General Hospital, Chongqing, China
3 Key Laboratory of Integrated Chinese and Western Medicine for Gastroenterology Research (Hebei), Shijiazhuang, China

* Corresponding Authors: JIANMING HE. Email: email; XI LIANG. Email: email

BIOCELL 2023, 47(12), 2671-2680. https://doi.org/10.32604/biocell.2023.043494

Abstract

Introduction: Collective cancer cell migration (CCCM) and epithelial-to-mesenchymal transition (EMT) play key roles in metastasis. This study reports that the colorectal carcinoma cell line LIM1863 is useful for the study of CCCM and EMT. Methods: Hematoxylin and eosin staining, scanning electron microscopy, transmission electron microscopy, and western blot analysis were performed. Results: LIM1863 automatically grew as spheroids in suspension and had important typical epithelial properties, including several layers of cells arranged around a central lumen, apical-basal polarity, and types of cell-cell junctions. Treatment with a combination of both TGF beta 1 and TNF alpha induced definite and distinct EMT, a spheroid changing phenotype to form a monolayer high-confluent patch without lumen, without polarity. Spontaneous CCCM occurred in spheroids. Flat EMT cells adhered to the base of a dish, exhibited persistent movement as a cluster of cells, and then shed, resulting in a cluster. All cells from one cluster undergoing CCCM died. Otherwise, all cells undergoing EMT disappeared and almost all cells located in the cell reservoir survived and proliferated. Conclusion: LIM1863 is an excellent cell line to study CCCM and EMT. The group of heterogeneous cells undergoing CCCM behaves like a supracellular unit.

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APA Style
WU, J., ZHI, Z., XU, W., LI, D., LI, Q. et al. (2023). LIM1863 is useful to explore collective cancer cell migration, and the group of heterogeneous cells undergoing collective migration behaves like a supracellular unit. BIOCELL, 47(12), 2671-2680. https://doi.org/10.32604/biocell.2023.043494
Vancouver Style
WU J, ZHI Z, XU W, LI D, LI Q, HAN Y, et al. LIM1863 is useful to explore collective cancer cell migration, and the group of heterogeneous cells undergoing collective migration behaves like a supracellular unit. BIOCELL . 2023;47(12):2671-2680 https://doi.org/10.32604/biocell.2023.043494
IEEE Style
J. WU et al., "LIM1863 is useful to explore collective cancer cell migration, and the group of heterogeneous cells undergoing collective migration behaves like a supracellular unit," BIOCELL , vol. 47, no. 12, pp. 2671-2680. 2023. https://doi.org/10.32604/biocell.2023.043494



cc This work is licensed under a Creative Commons Attribution 4.0 International License , which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
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