Home / Journals / BIOCELL / Vol.36, No.1, 2012
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  • Open Access

    REVIEW

    Review: Correlative microscopy of Purkinje cells

    ORLANDO J. CASTEJÓN*
    BIOCELL, Vol.36, No.1, pp. 1-29, 2012, DOI:10.32604/biocell.2012.36.001
    Abstract The Purkinje cell and their synaptic contacts have been described using (1) light microsocopy, (2) transmission and scanning electron microscopy, and freeze etching technique, (3) conventional and field emission scanning electron microscopy and cryofracture methods, (4) confocal laser scanning microscopy using intravital stain FM64, and (5) immunocytochemical techniques for Synapsin-I, PSD9-5, GluR1 subunit of AMPA receptors, N-cadherin, and CamKII alpha. The outer surface and inner content of plasma membrane, cell organelles, cytoskeleton, nucleus, dendritic and axonal processes have been exposed and analyzed in a three-dimensional view. The intramembrane morphology, in bi- and three-dimensional views, and immunocytochemical labeling of synaptic contacts… More >

  • Open Access

    ARTICLE

    Cryopreservation of Cyrtopodium hatschbachii Pabst (Orchidaceae) immature seeds by encapsulation-dehydration

    MAURO RODRIGO SURENCISKI*, EDUARDO ALBERTO FLACHSLAND, GRACIELA TERADA, LUIS AMADO MROGINSKI, HEBE YOLANDA REY
    BIOCELL, Vol.36, No.1, pp. 31-36, 2012, DOI:10.32604/biocell.2012.36.031
    Abstract The aim of the present study was to investigate the efficiency of the encapsulation-dehydration technique for cryopreservation of Cyrtopodium hastchbachii Pabst seeds. Immature seeds of this species were cryopreserved by an encapsulation-dehydration technique. Seeds of five immature pods, 120 days after pollination, were encapsulated in 3% calcium alginate matrix and pretreated in liquid medium supplemented with 0.08 M sucrose (24 h), 0.15 M sucrose (24 h), 0.25 M sucrose (48 h), 0.5 M sucrose (24 h) and 0.75 M sucrose (24 h) in shaker at 60 rpm. Alginate beads were dehydrated 5 h in silicagel and immersed in liquid nitrogen… More >

  • Open Access

    ARTICLE

    Optimization and comparison of two different 3D culture methods to prepare cell aggregates as a bioink for organ printing

    RANA IMANI1, SHAHRIAR HOJJATI EMAMI1, HOSSEIN FAKHRZADEH2, NAFISEH BAHEIRAEI1, ALI M SHARIFI* 2,3,4
    BIOCELL, Vol.36, No.1, pp. 37-45, 2012, DOI:10.32604/biocell.2012.36.037
    Abstract The ultimate goal of tissue engineering is to design and fabricate functional human tissues that are similar to natural cells and are capable of regeneration. Preparation of cell aggregates is one of the important steps in 3D tissue engineering technology, particularly in organ printing. Two simple methods, hanging drop (HD) and conical tube (CT) were utilized to prepare cell aggregates. The size and viability of the aggregates obtained at different initial cell densities and pre-culture duration were compared. The proliferative ability of the cell aggregates and their ability to spread in culture plates were also investigated. In both methods, the… More >

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