Table of Content

Open Access

ARTICLE

Cryopreservation of Cyrtopodium hatschbachii Pabst (Orchidaceae) immature seeds by encapsulation-dehydration

MAURO RODRIGO SURENCISKI*, EDUARDO ALBERTO FLACHSLAND, GRACIELA TERADA, LUIS AMADO MROGINSKI, HEBE YOLANDA REY
Instituto de Botánica del Nordeste (IBONE), CONICET–UNNE, Facultad de Ciencias Agrarias (UNNE), C.C. 209, (3400) Corrientes, Argentina.
* Address correspondence to: Mauro Rodrigo SURENCISKI. E-mail:

BIOCELL 2012, 36(1), 31-36. https://doi.org/10.32604/biocell.2012.36.031

Abstract

The aim of the present study was to investigate the efficiency of the encapsulation-dehydration technique for cryopreservation of Cyrtopodium hastchbachii Pabst seeds. Immature seeds of this species were cryopreserved by an encapsulation-dehydration technique. Seeds of five immature pods, 120 days after pollination, were encapsulated in 3% calcium alginate matrix and pretreated in liquid medium supplemented with 0.08 M sucrose (24 h), 0.15 M sucrose (24 h), 0.25 M sucrose (48 h), 0.5 M sucrose (24 h) and 0.75 M sucrose (24 h) in shaker at 60 rpm. Alginate beads were dehydrated 5 h in silicagel and immersed in liquid nitrogen for 12 h. Cryopreserved beads were thawed at 30°C for 1 min, rehydrated using the same liquid mediums [0.75 M sucrose (24 h), 0.5 M sucrose (24 h), 0.25 M sucrose (48 h) and 0.15 M sucrose (24 h)] and cultivated in half strength Murashige & Skoog medium (1962) with the addition of 2 g/L activated charcoal. Sixty four percent of seeds survived and developed into acclimatized plants after being cryopreserved. In this work, the encapsulation-dehydration technique was employed for first time in Cyrtopodium hatschbachii.

Keywords

Cryoconservation, liquid nitrogen, calcium alginate, activated charcoal, sucrose

Cite This Article

SURENCISKI, M. R., FLACHSLAND, E. A., TERADA, G., MROGINSKI, L. A., REY, H. Y. (2012). Cryopreservation of Cyrtopodium hatschbachii Pabst (Orchidaceae) immature seeds by encapsulation-dehydration. BIOCELL, 36(1), 31–36.

Citations




This work is licensed under a Creative Commons Attribution 4.0 International License , which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
  • 902

    View

  • 554

    Download

  • 0

    Like

Share Link

WeChat scan