Table of Content

Open Access iconOpen Access

ARTICLE

Cryopreservation of Cyrtopodium hatschbachii Pabst (Orchidaceae) immature seeds by encapsulation-dehydration

MAURO RODRIGO SURENCISKI*, EDUARDO ALBERTO FLACHSLAND, GRACIELA TERADA, LUIS AMADO MROGINSKI, HEBE YOLANDA REY

Instituto de Botánica del Nordeste (IBONE), CONICET–UNNE, Facultad de Ciencias Agrarias (UNNE), C.C. 209, (3400) Corrientes, Argentina.

* Address correspondence to: Mauro Rodrigo SURENCISKI. E-mail: email

BIOCELL 2012, 36(1), 31-36. https://doi.org/10.32604/biocell.2012.36.031

Abstract

The aim of the present study was to investigate the efficiency of the encapsulation-dehydration technique for cryopreservation of Cyrtopodium hastchbachii Pabst seeds. Immature seeds of this species were cryopreserved by an encapsulation-dehydration technique. Seeds of five immature pods, 120 days after pollination, were encapsulated in 3% calcium alginate matrix and pretreated in liquid medium supplemented with 0.08 M sucrose (24 h), 0.15 M sucrose (24 h), 0.25 M sucrose (48 h), 0.5 M sucrose (24 h) and 0.75 M sucrose (24 h) in shaker at 60 rpm. Alginate beads were dehydrated 5 h in silicagel and immersed in liquid nitrogen for 12 h. Cryopreserved beads were thawed at 30°C for 1 min, rehydrated using the same liquid mediums [0.75 M sucrose (24 h), 0.5 M sucrose (24 h), 0.25 M sucrose (48 h) and 0.15 M sucrose (24 h)] and cultivated in half strength Murashige & Skoog medium (1962) with the addition of 2 g/L activated charcoal. Sixty four percent of seeds survived and developed into acclimatized plants after being cryopreserved. In this work, the encapsulation-dehydration technique was employed for first time in Cyrtopodium hatschbachii.

Keywords


Cite This Article

APA Style
SURENCISKI, M.R., FLACHSLAND, E.A., TERADA, G., MROGINSKI, L.A., REY, H.Y. (2012). Cryopreservation of <i>cyrtopodium hatschbachii</i> pabst (orchidaceae) immature seeds by encapsulation-dehydration. BIOCELL, 36(1), 31-36. https://doi.org/10.32604/biocell.2012.36.031
Vancouver Style
SURENCISKI MR, FLACHSLAND EA, TERADA G, MROGINSKI LA, REY HY. Cryopreservation of <i>cyrtopodium hatschbachii</i> pabst (orchidaceae) immature seeds by encapsulation-dehydration. BIOCELL . 2012;36(1):31-36 https://doi.org/10.32604/biocell.2012.36.031
IEEE Style
M.R. SURENCISKI, E.A. FLACHSLAND, G. TERADA, L.A. MROGINSKI, and H.Y. REY "Cryopreservation of <i>Cyrtopodium hatschbachii</i> Pabst (Orchidaceae) immature seeds by encapsulation-dehydration," BIOCELL , vol. 36, no. 1, pp. 31-36. 2012. https://doi.org/10.32604/biocell.2012.36.031

Citations




cc This work is licensed under a Creative Commons Attribution 4.0 International License , which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
  • 1377

    View

  • 864

    Download

  • 0

    Like

Share Link