Home / Journals / BIOCELL / Vol.39, No.2-3, 2015
Table of Content
  • Open Access

    ARTICLE

    Carcinoembryonic antigen inhibits neutrophil activation by N-formyl-methionyl-leucyl-phenylalanine

    Anna PAŃCZYSZYN1 *, Anna KROP-WATOREK1,2, Maciej WIECZOREK1
    BIOCELL, Vol.39, No.2-3, pp. 1-4, 2015, DOI:10.32604/biocell.2015.39.001
    Abstract Carcinoembryonic antigen (CEA) is a surface glycoprotein expressed in human epithelial cells and is released from their surface, especially during colorectal cancer. Frequently, colorectal cancer is accompanied by inflammation, where tumor-infiltrating neutrophils play an important role. CEA was also found to be a strong chemotactic agent for neutrophils. The purpose of this study was to find out if CEA can enhance neutrophil priming and activation. Primed neutrophils were activated by N-formyl-methionyl-leucyl-phenylalanine (formyl-MLP) and the resulting oxidative burst was measured luminometrically. Unexpectedly, in vitro priming of neutrophils by CEA, alone or preceded by LPS, inhibited subsequent activation of these cells by… More >

  • Open Access

    ARTICLE

    Adenosine A1 receptor activation inhibits histamine release in gastric enterochromaffin-like cells

    Rafael Martins de OLIVEIRA, Carolina Bernardi STEFANI, Angélica Aparecida Antoniellis SILVEIRA, Marcelo Lima RIBEIRO, José PEDRAZZOLI Jr., Alessandra GAMBERO*
    BIOCELL, Vol.39, No.2-3, pp. 5-8, 2015, DOI:10.32604/biocell.2015.39.005
    Abstract Adenosine acts as a gastroprotective factor decreasing inflammation and reducing gastric acid secretion. Quantitative RT-PCR was used to determine the expression of the adenosine receptor genes (A1AR, A2AAR, A2BAR and A3AR) and that of the gastrin receptor B gene (CCKBR) in isolated, short-term cultured enterochromaffin-like (ECL) cells. Both the A1AR and the CCKBR genes were expressed at a level higher than the other genes. Also, the effect of 2-chloroadenosine, a stable agonist of A1 and A2A receptors, was explored on ECL cells, with a resulting inhibition of both basal and gastrin-stimulated histamine release. Also, dipropylcyclopentylxanthine (DPCPX), a selective A1 antagonist,… More >

  • Open Access

    ARTICLE

    Hepatocyte culture in a radial-flow bioreactor with plasma polypyrrole coated scaffolds

    Odin RAMÍREZ-FERNÁNDEZ1,*, Rafael GODÍNEZ1, Esmeralda ZUÑIGA-AGUILAR1, Luis E. GÓMEZ-QUIROZ2, María C. GUTIÉRREZ-RUIZ2, Juan MORALES3, Roberto OLAYO3
    BIOCELL, Vol.39, No.2-3, pp. 9-14, 2015, DOI:10.32604/biocell.2015.39.009
    Abstract We have designed and evaluated a radial-flow bioreactor for three-dimensional liver carcinoma cell culture on a new porous coated scaffold. We designed a culture chamber where a radial flow of culture medium was continuously delivered through it. Once this system was established, flow was simulated using flow dynamics software based on numeric methods to solve Navier-Stockes flow equations. Perfusion within cell culture scaffolds was simulated using a flow velocity of 7 mL/min and found that cell culture medium was distributed unhindered in the bioreactor chamber. Afterwards, the bioreactor was built according to the simulated design and was tested with liver… More >

  • Open Access

    ARTICLE

    An internal control applied to RT-PCR detection of HCV and HIV-1 in human pooled plasma and plasma-derived medicinal products

    Gonzalo RODRÍGUEZ-LOMBARDI1, Luciana REYNA2, María Susana VITALI1, Susana GENTI-RAIMONDI2
    BIOCELL, Vol.39, No.2-3, pp. 15-24, 2015, DOI:10.32604/biocell.2015.39.015
    Abstract A competitive internal control (IC) adapted to RT-PCR in-house assay was developed for HCV RNA detection in human pooled plasma. Also, it was applied in a multiplex RT-PCR for the HIV-1 and HCV RNA screening in human pooled plasma and plasma-derived products. A 258-bp PCR product from the 5´noncoding region of HCV genome was obtained. A competitive IC template was constructed by inserting a 52-bp double strand sequence into the NheI site of the 258-bp amplicon. This sequence was cloned and the obtained plasmid was used to generate a synthetic RNA. The IC/RNA was incorporated in in-house HCV and/or HIV… More >

  • Open Access

    ARTICLE

    Glypican-3 versus alpha-fetoprotein as a biomarker for hepatocellular carcinoma: a diagnostic meta-analysis

    Weiyu FU1, Hongyu LU2, Li LI3, Kefeng WU3, Yanping LI3, Yi LIU3,*, Jianli HU4,*
    BIOCELL, Vol.39, No.2-3, pp. 25-32, 2015, DOI:10.32604/biocell.2015.39.025
    Abstract Objective: To assess the diagnostic accuracy of serum GPC3 versus alpha-fetoprotein (AFP) for HCC by using the method of system review.
    Methods: PubMed and EMBASE were searched from its inception to 20, April 2014 for studies that compared diagnostic accuracy of serum GPC3 with AFP for HCC. Sensitivity, specificity and other measures were pooled using random-effects models. Summary receiver operating characteristic curve analysis was used to summarize the overall test performance.
    Results: Fourteen studies were included in this meta-analysis. Summary estimates for serum GPC3 and AFP in diagnosing HCC were as follows: sensitivity, 69% (95% confidence interval (CI), 56-80%) vs.… More >

  • Open Access

    ARTICLE

    Mouse cerebellar Purkinje cell damage induced by diphenylhydantoin acute intoxication

    Orlando J. CASTEJÓN*
    BIOCELL, Vol.39, No.2-3, pp. 33-40, 2015, DOI:10.32604/biocell.2015.39.033
    Abstract Twenty one days old Swiss albino mice that received diphenylhydantoin (25 mg/kg, i.p., daily for 15 days) progressively developed gait alterations, changes of behavior and cerebellar ataxia. Cerebellar slices were processed by conventional transmission electron microscopy. The body of Purkinje cells exhibited fragmented limiting plasma membranes, dilated nuclear envelopes, swelling and disassembly of nuclear pores, enlargement of rough and smooth endoplasmic reticulum and a notable detachment of membrane associated ribosomes, together with distorted vacuoles of smooth endoplasmic reticulum, bizarre shaped and swollen mitochondria with dilated cristae, as well as disrupted limiting lysosomal membranes. Degenerated axosomatic synapses apparently corresponding to basket… More >

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