Open Access
ARTICLE
Anna PAŃCZYSZYN1 *, Anna KROP-WATOREK1,2, Maciej WIECZOREK1
BIOCELL, Vol.39, No.2-3, pp. 1-4, 2015, DOI:10.32604/biocell.2015.39.001
Abstract Carcinoembryonic antigen (CEA) is a surface glycoprotein expressed in human epithelial cells
and is released from their surface, especially during colorectal cancer. Frequently, colorectal cancer is accompanied by inflammation, where tumor-infiltrating neutrophils play an important role. CEA was also found to be
a strong chemotactic agent for neutrophils. The purpose of this study was to find out if CEA can enhance neutrophil priming and activation. Primed neutrophils were activated by N-formyl-methionyl-leucyl-phenylalanine
(formyl-MLP) and the resulting oxidative burst was measured luminometrically. Unexpectedly, in vitro priming
of neutrophils by CEA, alone or preceded by LPS, inhibited subsequent activation of these cells by… More >
Open Access
ARTICLE
Rafael Martins de OLIVEIRA, Carolina Bernardi STEFANI, Angélica Aparecida Antoniellis SILVEIRA, Marcelo Lima RIBEIRO, José PEDRAZZOLI Jr., Alessandra GAMBERO*
BIOCELL, Vol.39, No.2-3, pp. 5-8, 2015, DOI:10.32604/biocell.2015.39.005
Abstract Adenosine acts as a gastroprotective factor decreasing inflammation and reducing gastric
acid secretion. Quantitative RT-PCR was used to determine the expression of the adenosine receptor genes
(A1AR, A2AAR, A2BAR and A3AR) and that of the gastrin receptor B gene (CCKBR) in isolated, short-term
cultured enterochromaffin-like (ECL) cells. Both the A1AR and the CCKBR genes were expressed at a level higher than the other genes. Also, the effect of 2-chloroadenosine, a stable agonist of A1 and A2A receptors, was
explored on ECL cells, with a resulting inhibition of both basal and gastrin-stimulated histamine release. Also,
dipropylcyclopentylxanthine (DPCPX), a selective A1 antagonist,… More >
Open Access
ARTICLE
Odin RAMÍREZ-FERNÁNDEZ1,*, Rafael GODÍNEZ1, Esmeralda ZUÑIGA-AGUILAR1, Luis E. GÓMEZ-QUIROZ2, María C. GUTIÉRREZ-RUIZ2, Juan MORALES3, Roberto OLAYO3
BIOCELL, Vol.39, No.2-3, pp. 9-14, 2015, DOI:10.32604/biocell.2015.39.009
Abstract We have designed and evaluated a radial-flow bioreactor for three-dimensional liver carcinoma
cell culture on a new porous coated scaffold. We designed a culture chamber where a radial flow of culture medium was continuously delivered through it. Once this system was established, flow was simulated using flow dynamics software based on numeric methods to solve Navier-Stockes flow equations. Perfusion within cell culture
scaffolds was simulated using a flow velocity of 7 mL/min and found that cell culture medium was distributed
unhindered in the bioreactor chamber. Afterwards, the bioreactor was built according to the simulated design
and was tested with liver… More >
Open Access
ARTICLE
Gonzalo RODRÍGUEZ-LOMBARDI1, Luciana REYNA2, María Susana VITALI1, Susana GENTI-RAIMONDI2
BIOCELL, Vol.39, No.2-3, pp. 15-24, 2015, DOI:10.32604/biocell.2015.39.015
Abstract A competitive internal control (IC) adapted to RT-PCR in-house assay was developed for HCV
RNA detection in human pooled plasma. Also, it was applied in a multiplex RT-PCR for the HIV-1 and HCV
RNA screening in human pooled plasma and plasma-derived products. A 258-bp PCR product from the 5´noncoding region of HCV genome was obtained. A competitive IC template was constructed by inserting a 52-bp
double strand sequence into the NheI site of the 258-bp amplicon. This sequence was cloned and the obtained
plasmid was used to generate a synthetic RNA. The IC/RNA was incorporated in in-house HCV and/or HIV… More >
Open Access
ARTICLE
Weiyu FU1, Hongyu LU2, Li LI3, Kefeng WU3, Yanping LI3, Yi LIU3,*, Jianli HU4,*
BIOCELL, Vol.39, No.2-3, pp. 25-32, 2015, DOI:10.32604/biocell.2015.39.025
Abstract Objective: To assess the diagnostic accuracy of serum GPC3 versus alpha-fetoprotein (AFP) for
HCC by using the method of system review.
Methods: PubMed and EMBASE were searched from its inception to 20, April 2014 for studies that compared
diagnostic accuracy of serum GPC3 with AFP for HCC. Sensitivity, specificity and other measures were pooled
using random-effects models. Summary receiver operating characteristic curve analysis was used to summarize
the overall test performance.
Results: Fourteen studies were included in this meta-analysis. Summary estimates for serum GPC3 and AFP in
diagnosing HCC were as follows: sensitivity, 69% (95% confidence interval (CI), 56-80%) vs.… More >
Open Access
ARTICLE
Orlando J. CASTEJÓN*
BIOCELL, Vol.39, No.2-3, pp. 33-40, 2015, DOI:10.32604/biocell.2015.39.033
Abstract Twenty one days old Swiss albino mice that received diphenylhydantoin (25 mg/kg, i.p., daily for
15 days) progressively developed gait alterations, changes of behavior and cerebellar ataxia. Cerebellar slices were
processed by conventional transmission electron microscopy. The body of Purkinje cells exhibited fragmented
limiting plasma membranes, dilated nuclear envelopes, swelling and disassembly of nuclear pores, enlargement
of rough and smooth endoplasmic reticulum and a notable detachment of membrane associated ribosomes, together with distorted vacuoles of smooth endoplasmic reticulum, bizarre shaped and swollen mitochondria with
dilated cristae, as well as disrupted limiting lysosomal membranes. Degenerated axosomatic synapses apparently
corresponding to basket… More >
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