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Adenosine A1 receptor activation inhibits histamine release in gastric enterochromaffin-like cells

Rafael Martins de OLIVEIRA, Carolina Bernardi STEFANI, Angélica Aparecida Antoniellis SILVEIRA, Marcelo Lima RIBEIRO, José PEDRAZZOLI Jr., Alessandra GAMBERO*

Clinical Pharmacology and Gastroenterology Unit, São Francisco University Medical School, Bragança Paulista, SP, Brazil.

* Address correspondence to:Alessandra Gambero, email

BIOCELL 2015, 39(2-3), 5-8. https://doi.org/10.32604/biocell.2015.39.005

Abstract

Adenosine acts as a gastroprotective factor decreasing inflammation and reducing gastric acid secretion. Quantitative RT-PCR was used to determine the expression of the adenosine receptor genes (A1AR, A2AAR, A2BAR and A3AR) and that of the gastrin receptor B gene (CCKBR) in isolated, short-term cultured enterochromaffin-like (ECL) cells. Both the A1AR and the CCKBR genes were expressed at a level higher than the other genes. Also, the effect of 2-chloroadenosine, a stable agonist of A1 and A2A receptors, was explored on ECL cells, with a resulting inhibition of both basal and gastrin-stimulated histamine release. Also, dipropylcyclopentylxanthine (DPCPX), a selective A1 antagonist, prevented the inhibitory effects of 2-chloroadenosine, suggesting the effect of 2-chloroadenosine is mediated by A1 receptors. It is concluded that isolated, short-term cultured ECL cells are a suitable model for studies relating gene expression and function, and that the gastroprotective actions of adenosine are at least partly mediated through A1 receptors.

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APA Style
OLIVEIRA, R.M.D., STEFANI, C.B., SILVEIRA, A.A.A., RIBEIRO, M.L., Jr., J.P. et al. (2015). Adenosine A1 receptor activation inhibits histamine release in gastric enterochromaffin-like cells. BIOCELL, 39(2-3), 5-8. https://doi.org/10.32604/biocell.2015.39.005
Vancouver Style
OLIVEIRA RMD, STEFANI CB, SILVEIRA AAA, RIBEIRO ML, Jr. JP, GAMBERO A. Adenosine A1 receptor activation inhibits histamine release in gastric enterochromaffin-like cells. BIOCELL . 2015;39(2-3):5-8 https://doi.org/10.32604/biocell.2015.39.005
IEEE Style
R.M.D. OLIVEIRA, C.B. STEFANI, A.A.A. SILVEIRA, M.L. RIBEIRO, J.P. Jr., and A. GAMBERO "Adenosine A1 receptor activation inhibits histamine release in gastric enterochromaffin-like cells," BIOCELL , vol. 39, no. 2-3, pp. 5-8. 2015. https://doi.org/10.32604/biocell.2015.39.005



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