Home / Journals / BIOCELL / Vol.46, No.7, 2022
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Ischaemic stroke, associated with blood-brain barrier breakdown, continues to be a leading cause of mortality and morbidity. Thrombolysis with recombinant tissue plasminogen activator remains the only approved pharmacotherapy for this disease but is only given to few eligible patients due to its short therapeutic window. Endothelial progenitor cells (EPCs), released by bone marrow in response to ischaemic injury, are important endogenous elements that replace damaged endothelial cells to restore barrier integrity. Outgrowth endothelial cells, generated by ex vivo expansion of EPCs, may serve as efficacious off-the-shelf therapeutics. However, several questions concerning their dose and delivery route need addressing before their routine application in clinical practice.
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  • Open Access

    VIEWPOINT

    Prognostic, diagnostic and therapeutic potential of endothelial progenitor cells for patients with ischaemic stroke: Hype or Hope

    ULVI BAYRAKTUTAN*
    BIOCELL, Vol.46, No.7, pp. 1593-1598, 2022, DOI:10.32604/biocell.2022.018679
    Abstract Ischaemic stroke is a debilitating disease with immense personal, societal and economic impact. Thrombolysis with recombinant tissue plasminogen activator remains the only approved pharmacotherapy for this disease. As each year less than 1% of eligible patients receive this therapy worldwide, efficacious new therapeutics are desperately needed. Emerging evidence suggest endothelial progenitor cells (EPCs), capable of repairing damaged vasculature, as one such therapeutics. However, questions regarding their optimal dose, delivery route and in vivo survivability remain largely unanswered. Outgrowth endothelial cells, generated in large numbers by ex vivo expansion of EPCs, enable effective assessment of these issues and may eventually serve… More >

  • Open Access

    REVIEW

    Ferroptosis molecular inducers: A future direction for malignant tumor chemotherapy

    ZIQIAN WANG1,2,#, YAQI LI1,2,#, DONGYANG WANG1,2, YINGQIANG SHEN1,3,4,5,*
    BIOCELL, Vol.46, No.7, pp. 1599-1611, 2022, DOI:10.32604/biocell.2022.018530
    (This article belongs to this Special Issue: Single Cell Technologies and Molecular Mechanisms of Diseases)
    Abstract Iron-dependent ferroptosis is a form of cell death dependent on iron levels. Cells that undergo ferroptosis have glutathione (GSH) deficiency, reduced Glutathione peroxidase-4 (GPX4) activity and intracellular lipid peroxidation, Mitochondria, lysosomes and many signal pathways are involved in the regulation of ferroptosis. More importantly, many tumor cells resistant to other cell death methods exhibit sensitivity to ferroptosis. Moreover, over recent years, a number of ferroptosis-induced drugs have been recommended for the treatment of malignant tumors. Therefore, the study of ferroptosis is of great significance for future cancer treatments. In this review, we discussed the metabolic process of ferroptosis, the role… More >

  • Open Access

    REVIEW

    Screening and evaluation of chilli (Capsicum annuum L.) genotypes for waterlogging tolerance at seedling stage

    MD. REZWAN MOLLA1,2,4, MD. MOTIAR ROHMAN2,*, MD. ROBYUL ISLAM1,2, MIRZA HASANUZZAMAN3,*, LUTFUL HASSAN4
    BIOCELL, Vol.46, No.7, pp. 1613-1627, 2022, DOI:10.32604/biocell.2022.019243
    (This article belongs to this Special Issue: Advances in Plant Cell Biology Research)
    Abstract Waterlogging is an illustrious abiotic stress and the constrictions it enforces on plant roots have negative effects on growth and development. This study was undertaken to investigate waterlogging stress tolerant potential in chilli (Capsicum annum L.) genotypes through evaluating morphological, physiological, biochemical and anatomical parameters. Thirty-five days old seedlings of 10 chilli genotypes were exposed to waterlogging stress maintaining water height 3–5 cm over the soil surface artificially for three days. This duration (36–38 DAE) was termed as waterlogging period, and subsequent withdrawal of waterlogging condition (39–45 DAE) was regarded as a recovery phase. Based on their survival performance, two… More >

  • Open Access

    VIEWPOINT

    Microenvironment and cell mechanics

    VAN-CHIEN BUI*
    BIOCELL, Vol.46, No.7, pp. 1629-1632, 2022, DOI:10.32604/biocell.2022.018364
    Abstract Microenvironment contains biophysical and biochemical elements to maintain survival, growth, proliferation, and differentiation of cells. Any change can lead to cell response to the mechanical forces, which can be described by elasticity. It is an indicator of a cell’s state since it plays an important role in many cellular processes. In many cases, cell elasticity is measured by using discontinuous manner, which may not allow elucidating real-time activity of individual live cells in physiological condition or cell response against microenvironmental changes. I argue that measuring cell elasticity using continuously repetitive nanoindentation technique is important that should be considered. As an… More >

  • Open Access

    VIEWPOINT

    Expression analysis of OsSERK, OsLEC1 and OsWOX4 genes in rice (Oryza sativa L.) callus during somatic embryo development

    SITI NABILAH1,2; TRI HANDOYO2,3; KYUNG-MIN KIM4; MOHAMMAD UBAIDILLAH2,*
    BIOCELL, Vol.46, No.7, pp. 1633-1641, 2022, DOI: 10.32604/biocell.2022.019111
    (This article belongs to this Special Issue: Tumor Microenvironment and Cytoskeletal Dynamics)
    Abstract Somatic embryogenesis is an asexual reproduction process that occurs in many plant species, including rice. This process contains several totipotency markers such as Somatic Embryogenesis Receptor-like Kinase (SERK), Leafy Cotyledon1 (LEC1) and WUSCHEL-Related Homeobox4 (WOX4) and also a helpful model for embryo development and clones and transformations. Here, we report the gene expression during somatic embryo development correlates with regeneration frequency in 14 Javanica rice (pigmented and non-pigmented) using modifified N6 media supplemented with Kinetin (2.0 mg/L) and NAA (1.0 mg/L). Although there have been advances in understanding the genetic basis of somatic embryogenesis in other varieties, rice is still… More >

  • Open Access

    VIEWPOINT

    Co-regulator NCOA5 and cancer

    YUANYUAN CHEN, SHUAISHUAI CUI, YUNFEI GUO, DAHU CHEN*
    BIOCELL, Vol.46, No.7, pp. 1643-1650, 2022, DOI:10.32604/biocell.2022.019142
    (This article belongs to this Special Issue: Biochemical and Epigenetics Changes in Health and Disease)
    Abstract NCOA5 encodes a co-regulator for estrogen receptors (ERα and ERβ), orphan nuclear receptors (REV-ERBα and REV-ERBβ) and liver X receptor. It can influence many cellular processes by either promoting or inhibiting gene expression through its two important functional motifs: LxxLL (co-activator) and ΦxxΦΦ (co-repressor). Many reports have revealed the important roles of NCOA5 in diseases, such as diabetes, reproductive defects and autoimmune disease. In this review, we focus on its function in cancers and summary the current research progresses regarding its different roles in various cancers. More >

  • Open Access

    ARTICLE

    Hypoxia induced apoptosis of rat gastric mucosal cells by activating autophagy through HIF-1α/TERT/mTORC1 pathway

    YAPING WANG1,2,#,*; XIAOHONG XUE2,#; ZHEN ZHAO1,#; XIAOLIN LI2; ZHIYONG ZHU1
    BIOCELL, Vol.46, No.7, pp. 1651-1659, 2022, DOI:10.32604/biocell.2022.018559
    (This article belongs to this Special Issue: Biochemical and Epigenetics Changes in Health and Disease)
    Abstract The pathogenesis of high altitude-related gastric mucosal injury remains poorly understood, this study aimed to investigate the role of autophagy in hypoxia-induced apoptosis of rat gastric mucosal cells. Rats were randomized into four groups which were maintained at an altitude of 400 m (P) or received no treatment (H), autophagy inducer rapamycin (H+AI) or autophagy inhibitor 3-MA (H+AB) at an altitude of 4,300 m for 1, 7, 14 and 21 days, respectively, and the morphology, ultrastructure, autophagy, and apoptosis of gastric mucosal tissues were examined. Gastric mucosal epithelial cells CC-R039 were cultured under conditions of normoxia, 2% O2 (hypoxia), or… More >

  • Open Access

    ARTICLE

    Weighted gene co-expression network analysis identifies a novel immune-related gene signature and nomogram to predict the survival and immune infiltration status of breast cancer

    JUNXIA LIU1, KE PANG2, FEI HE2,*
    BIOCELL, Vol.46, No.7, pp. 1661-1673, 2022, DOI:10.32604/biocell.2022.018023
    (This article belongs to this Special Issue: Decoding Gene (including circRNA, lincRNA miRNA and mRNA) Expression)
    Abstract Breast cancer is one of the most common cancers in the world and seriously threatens the health of women worldwide. Prognostic models based on immune-related genes help to improve the prognosis prediction and clinical treatment of breast cancer patients. In the study, we used weighted gene co-expression network analysis to construct a co-expression network to screen out highly prognostic immune-related genes. Subsequently, the prognostic immune-related gene signature was successfully constructed from highly immune-related genes through COX regression and LASSO COX analysis. Survival analysis and time receiver operating characteristic curves indicate that the prognostic signature has strong predictive performance. And we… More >

  • Open Access

    ARTICLE

    Identification of differential mRNA and lncRNA expression in AcMNPV-infected Sf9 cells

    TIEJUN ZHAO, RIQIANG DENG*, MENGQIU CHEN, XUNZHANG WANG
    BIOCELL, Vol.46, No.7, pp. 1675-1686, 2022, DOI:10.32604/biocell.2022.018166
    (This article belongs to this Special Issue: Decoding Gene (including circRNA, lincRNA miRNA and mRNA) Expression)
    Abstract Sf9Sf9 are the ovarian cells of Spodoptera frugiperda that is the host of Autographa californica multiple nucleopolyhedrovirus (AcMNPV), and hence can serve as an effective test vehicle to understand the AcMNPV infection mechanism. In this study, through high-throughput sequencing technology using samples collected from Sf9 cells at different time points after AcMNPV infection, 3463 pieces of time-series differentially expressed RNA (1,200 mRNA and 2,263 lncRNA) are identified and justified by experimental verification of randomly selected samples from them, proving the validity of the bioinformatical analysis on this topic. Functional enrichment analysis and target prediction are performed on those differentially expressed… More >

  • Open Access

    ARTICLE

    Identification of key long noncoding RNAs and their biological functions in hepatocellular carcinoma

    FEI CHEN1,2, LIANG WANG3,*, YUHONG LI1,2,*
    BIOCELL, Vol.46, No.7, pp. 1687-1696, 2022, DOI:10.32604/biocell.2022.018078
    (This article belongs to this Special Issue: Noncoding RNAs & Associated Human Diseases)
    Abstract Long noncoding RNAs (lncRNAs) are vital regulators in tumorigenesis and metastasis. However, the pathological role of lncRNAs in hepatocellular carcinoma (HCC) is still unclear. In this study, we filtered out three lncRNAs from The Cancer Genome Atlas (TCGA) data that were screened for basic expression and clinical research. We selected lncRNA-NEAT1 for further study to explore its function in HCC progression and its regulatory mechanism. We identified three differentially expressed lncRNAs (DElncRNAs) in tumor and adjacent normal tissues from the TCGA library using data mining methods: lncRNA-NEAT1, lncRNA-MAGI2-AS3 and lncRNA-HCG11. Their basic expression levels were detected by qPCR. Then, we… More >

  • Open Access

    ARTICLE

    Butyrate inhibits the bovine rumen epithelial cell proliferation via downregulation of positive regulators at G0/G1 phase checkpoint

    KANG ZHAN#, MAOCHENG JIANG#, TIANYU YANG, ZIXUAN HU, GUOQI ZHAO*
    BIOCELL, Vol.46, No.7, pp. 1697-1704, 2022, DOI:10.32604/biocell.2022.019068
    (This article belongs to this Special Issue: Role of Single Cell Protein in Animal Nutrition)
    Abstract Short-chain fatty acids (SCFAs) butyrate promote the postnatal rumen epithelial development and maturation in ruminants. However, molecular mechanisms of effects of butyrate on the bovine rumen epithelial cells (BRECs) proliferation remain elusive. Therefore, purpose of this study was to investigate the effects of butyrate on the expression of genes and proteins at G0/G1 and S phase of BRECs cycle. Our results showed that BRECs treated with butyrate inhibited (P < 0.05) the proliferation of BRECs, relatively to control. Flow cytometric assays revealed that butyrate triggers the BRECs cycle arrest at the G0/G1 phase. qRT-PCR analyses of mRNA level of genes… More >

  • Open Access

    ARTICLE

    Characterization of three-dimensional multipotent adipose-derived stem cell spheroids

    HONGYANG LI1,2, CHAN WANG4, SHIWEI LIU1, YONGLONG GUO1,3,4,*, JIANSU CHEN1,3,4,*
    BIOCELL, Vol.46, No.7, pp. 1705-1716, 2022, DOI:10.32604/biocell.2022.018442
    (This article belongs to this Special Issue: Mesenchymal Stem Cells, Secretome and Biomaterials: Regenerative Medicine Application)
    Abstract Human adipose stem cells (hADSCs) are reliable sources for cell therapy. However, the clinical applications are limited by the decrease in activity during in vitro culture. We used a knockout serum replacement (KSR) medium, Eppendorf (EP) tube culture, and a simulated microgravity (SMG) culture system to establish hADSC spheroids. We found that hADSCs aggregated and formed spheroids in the KSR culture medium. The EP tube culture method revealed many biological cell characteristics, such as good cell viabilities, rough surfaces, polar growth, fusion phenomenon, and injectability. The findings show its advantages for hADSCs spherical cultures. When cultured in SMG, hADSC spheroids… More >

  • Open Access

    ARTICLE

    Long non-coding RNA MIR22HG inhibits the adipogenesis of human bone marrow mesenchymal stem cells with the involvement of Wnt/β-catenin pathway

    CHANYUAN JIN1,4,#, ZIYAO ZHUANG2,4,#, LINGFEI JIA3,4,*, YUNFEI ZHENG2,4,*
    BIOCELL, Vol.46, No.7, pp. 1717-1724, 2022, DOI:10.32604/biocell.2022.018706
    Abstract Osteoporosis is a frequently occurring bone remodeling disorder worldwide with one characteristic being decreasing bone mineral density and a predisposition to bone fracture, which diminishes patients’ quality of life. Several studies showed that imbalance between the osteogenesis and adipogenesis of bone marrow mesenchymal stem cells (BMSCs) took part in the development of osteoporosis. In previous study, we found MIR22HG regulated the osteogenesis of human BMSCs positively. In this study, we found that MIR22HG was decreased during the adipogenesis of human BMSCs and exerted negative effects on adipogenesis with the involvement of Wnt/β-catenin signaling pathway both in vitro and in vivo.… More >

  • Open Access

    ARTICLE

    Divicine induces endothelial cells injury and its potential mechanism

    LONG SU#, ZHEXUAN LIN#, HUI LI, HONGJUN LUO, WENHONG LUO*
    BIOCELL, Vol.46, No.7, pp. 1725-1732, 2022, DOI:10.32604/biocell.2022.018508
    Abstract Divicine is an active pyrimidine aglycone, generated from vicine by the enzyme β-glucosidase upon ingestion of fava beans. In this study, we investigated the effect of divicine on cultured human umbilical vein endothelial cells (HUVECs) and explored the potential mechanisms. Incubation HUVECs with 18.5–85.1 μM divicine resulted in a concentration and time dependent decrease of cell viability, followed by decrease of cellular reduced glutathione, as well as increase of reactive oxygen species (ROS), malondialdehyde (MDA), and labile iron pool. Transmission electron microscopy confirmed that the divicine treated HUVECs’ mitochondria had shrunk. Importantly, the administration of desferrioxamine, an iron chelator, to… More >

  • Open Access

    ARTICLE

    Insight into the characteristics of an important evolutionary model bird (Geospiza magnirostris) mitochondrial genome through comparison

    ZHENGGANG XU1,2,3, LIANG WU3, JIAHAO CHEN1, YUNLIN ZHAO3, CHONGXUAN HAN1, TIAN HUANG2, GUIYAN YANG1,*
    BIOCELL, Vol.46, No.7, pp. 1733-1746, 2022, DOI:10.32604/biocell.2022.015784
    Abstract Darwin’s finches are the most classic case of evolution. Early studies on the evolution of this species were mainly based on morphology. Until now, the mitochondrial genome of Geospiza magnirostris has been sequenced and the study explored the characteristics of the complete genome of G. magnirostris and verified the evolutionary position of it. The 13 PCGs initiated by ATN codons. The stop codons of three PCGs (ND2, COX3 and ND4) were incomplete, with only T- or TA- replacing complete form TAA or TAG. All the tRNA genes expressed a typical cloverleaf secondary structure, except for tRNASer1(AGY), whose dihydrouridine (DHU) arm… More >

  • Open Access

    VIEWPOINT

    A need for stratification of metastasis samples according to secondary site in gene expression studies

    IVANA SAMARŽIJA1,2,*
    BIOCELL, Vol.46, No.7, pp. 1747-1750, 2022, DOI:10.32604/biocell.2022.018784
    Abstract Comparisons of gene expression profiles between primary tumors and metastasis have revealed genes that are implicated in metastasis formation. However, gene expression studies conducted on metastasis samples from the same primary site usually do not discriminate between different secondary sites. Although the change in the expression of number of genes is expected to be common to metastasis from the same primary but different secondary sites, herein the data that point to substantial differences are presented. Furthermore, the reciprocal communication between metastatic and host cells that is influencing these differences is outlined to emphasize the need for stratification of metastasis samples… More >

  • Open Access

    ARTICLE

    Genome-wide identification of U-box gene family and expression analysis under abiotic stresses in Salvia miltiorrhiza

    JIANMIN PAN1, XIN LI1, JUANJUAN LI1, HUI LIU2, ZHUONI HOU1, ZONGQI YANG1, LING XU1,*
    BIOCELL, Vol.46, No.7, pp. 1751-1762, 2022, DOI:10.32604/biocell.2022.019455
    (This article belongs to this Special Issue: Physiology and Molecular Biology of Plant Stress Tolerance)
    Abstract Plant U-box (PUB) E3 ubiquitin ligases play important roles in hormone signaling pathways and in response to different abiotic stresses, but little is known about U-box genes in Danshen (root of Salvia miltiorrhiza Bunge). Here, we identified and characterized 70 SmPUB genes based on its genome sequence. Phylogenetic analysis of U-box genes from S. miltiorrhiza and Arabidopsis suggested that they can be clustered into seven subgroups (I–VII). Typical U-box domains were found in all identified SmPUB genes through the analysis of conserved motifs. Moreover, qRT-PCR was applied to analyze the relative expression levels of U-box genes in S. miltiorrhiza roots… More >

  • Open Access

    ARTICLE

    Exogenous melatonin alleviated growth inhibition and oxidative stress induced by drought stress in apple rootstock

    MEIGE WANG, JUAN GONG, CHUNHUI SONG, ZHENGYANG WANG, SHANGWEI SONG, JIAN JIAO, MIAOMIAO WANG, XIANBO ZHANG*, TUANHUI BAI*
    BIOCELL, Vol.46, No.7, pp. 1763-1770, 2022, DOI:10.32604/biocell.2022.018934
    (This article belongs to this Special Issue: Physiology and Molecular Biology of Plant Stress Tolerance)
    Abstract

    Drought stress is one of the major environmental obstacles that limit the production and development of apples (Malus domestica Borkh.). The role of melatonin is well known in the protection of plants under environmental stresses. In this study, we investigated the effect of melatonin on apple rootstock M. hupehensis Rehd under drought stress. The results showed that drought inhibited the growth of M. hupehensis and dramatically reduced root surface area, root volume, the number of tips and forks, and root diameter. Drought-induced growth inhibition was significantly decreased by adding melatonin. Net photosynthetic rate (Pn), intercellular CO2 concentration (Ci), stomatal conductance… More >

  • Open Access

    ARTICLE

    Biomonitoring of endosulfan toxicity in human

    SANTOSH KUMAR KARN1, ADITYA UPADHYAY2, AWANISH KUMAR2,*
    BIOCELL, Vol.46, No.7, pp. 1771-1777, 2022, DOI:10.32604/biocell.2022.018845
    (This article belongs to this Special Issue: Advances in Plant Cell Biology Research)
    Abstract Chemicals are comprehensively used worldwide to control herbs, weeds, pests, and other competing agents with various growing crops. The consumption of crops grown with these chemicals (even in small quantities) can upshot into accumulation in the human body. People can accidentally inhale these hazardous chemicals if they are in an area where they were applied. These chemicals can be ingested in a human with contaminated food and drinks. Ultimately it causes various adverse effects (chronic toxicity, teratogenic, mutagenic, carcinogenic effect, reproductive, and organ toxicity) on human health. Among the pool of these chemicals used as pesticides in the environment, exposure… More >

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