Home / Journals / BIOCELL / Vol.45, No.6, 2021
Table of Content

On the Cover


Gene signatures associated with clinical traits identified by Weighted gene coexpression network analysis (WGCNA) guide the recent development of biomarkers for the diagnosis and function mechanism exploration of cancers. A 9-gene signature related to both stemness index (mRNAsi) and prognosis of hepatocellular carcinoma (HCC) is identified, which may control the metastasis and drug resistance in HCC.
View this paper

  • Open Access

    ARTICLE

    A novel nine gene signature integrates stemness characteristics associated with prognosis in hepatocellular carcinoma

    XINGHONG YAO1,2,#, CHENGQUAN LU3,#, JUNYI SHEN1, WENLI JIANG1, YAN QIU1, YE ZENG1,*, LIANG LI1,*
    BIOCELL, Vol.45, No.6, pp. 1425-1448, 2021, DOI:10.32604/biocell.2021.017289
    Abstract Cancer stem cells (CSCs) are heterogeneous with self-renewal and differentiation ability. The mRNA expression-based stemness index (mRNAsi) described the similarity between tumor cells and CSCs, which is positively associated with the poor prognosis of cancer patients. However, the key prognostic genes related to mRNAsi in hepatocellular carcinoma (HCC) remains unclear. A 9-gene signature related to mRNAsi and HCC prognosis including PSMG3, SNRPD1, DTYMK, PIGU, NME1, TXNL4A, IPO4, PES1, and REXO4 was obtained. High expression of this signature indicates poor prognosis of HCC. PIGU was an independent prognostic factor of HCC, which was significantly associated with progression of HCC. Among them,… More >

  • Open Access

    VIEWPOINT

    Real-Time analysis of exosome secretion of single cells with single molecule imaging

    PENGFEI ZHANG1, SHAOPENG WANG1,2,*
    BIOCELL, Vol.45, No.6, pp. 1449-1451, 2021, DOI:10.32604/biocell.2021.017607
    (This article belongs to this Special Issue: Single Cell Analysis: Technology and Application)
    Abstract The exosome-mediated response can promote or restrain the diseases by regulating the intracellular pathways, making the exosome become an effective marker for diagnosis and therapeutic control at the single-cell level. However, real-time analysis is hard to be achieved with traditional approaches because the exosomes usually need to be enriched by ultracentrifugation for a measurable signal-to-noise ratio. Recently developed label-free single-molecule imaging approaches may become an real-time quantitative tool for the analysis of single exosomes and related secretion behaviors of single living cells owing to their extreme sensitivity. More >

  • Open Access

    REVIEW

    Implications of enolase in the RANKL-mediated osteoclast activity following spinal cord injury

    RAMSHA SHAMS1,2, NAREN L. BANIK1,2,3,*, AZIZUL HAQUE1,*
    BIOCELL, Vol.45, No.6, pp. 1453-1457, 2021, DOI:10.32604/biocell.2021.017659
    Abstract Spinal Cord Injury (SCI) is a debilitating condition characterized by damage to the spinal cord, resulting in loss of function, mobility, and sensation. Although increasingly prevalent in the US, no FDA-approved therapy exists due to the unfortunate complexity of the condition, and the difficulties of SCI may be furthered by the development of SCI-related complications, such as osteoporosis. SCI demonstrates two crucial stages for consideration: the primary stage and the secondary stage. While the primary stage is suggested to be immediate and irreversible, the secondary stage is proposed as a promising window of opportunity for therapeutic intervention. Enolase, a metabolic… More >

  • Open Access

    VIEWPOINT

    Proteogenomics for pediatric brain cancer

    MARGARET SIMONIAN*
    BIOCELL, Vol.45, No.6, pp. 1459-1463, 2021, DOI:10.32604/biocell.2021.017369
    Abstract Pediatric central nervous system tumors are the most common tumors in children, it constitute 15%–20% of all malignancies in children and are the leading cause of cancer related deaths in children. Proteogenomics is an emerging field of biological research that utilizes a combination of proteomics, genomics, and transcriptomics to aid in the discovery and identification of biomarkers for diagnosis and therapeutic purposes. Integrative proteogenomics analysis of pediatric tumors identified underlying biological processes and potential treatments as well as the functional effects of somatic mutations and copy number variation driving tumorigenesis. More >

  • Open Access

    REVIEW

    Delineating the role of phytocompounds against anti-bacterial drug resistance–An update

    REKHA GAHTORI1,#, MOSLEH MOHAMMAD ABOMUGHAID2,#, NIDHI NEGI3, SARAVANAN KRISHNAN4, SUGAPRIYA DHANASEKARAN5, SOUMYA PANDIT6, KANU PRIYA6, DILLIP KUMAR BISHI7,*, RAM PRASAD8, PIYUSH KUMAR GUPTA6,*
    BIOCELL, Vol.45, No.6, pp. 1465-1477, 2021, DOI:10.32604/biocell.2021.016484
    Abstract Antibacterial resistance developed by bacteria due to the unlimited use of antibiotics has posed a challenge for human civilization. This kind of problem is not limited to India only, but it is a global concern. Nowadays, many treatments and medicines for bacterial diseases have been developed. However, they possess some drawbacks. Therefore, the alternative medicine has been used to target the drug resistant mechanisms and such medicines have less side effects which is becoming necessary. Natural products have traditionally or historically been of importance for the development of antibacterial agents and are also known to overcome bacterial drug resistance by… More >

  • Open Access

    REVIEW

    Nanoscale interactions between the nicotinic acetylcholine receptor and cholesterol

    FRANCISCO J. BARRANTES#,*
    BIOCELL, Vol.45, No.6, pp. 1479-1484, 2021, DOI:10.32604/biocell.2021.016502
    Abstract Cholesterol is a major lipid in biological membranes. It not only plays a structural role but also modulates a wide range of functional properties of neurotransmitter and hormone receptors and ion channels. The membraneembedded segments of the paradigm neurotransmitter receptor for acetylcholine (nAChR) contain linear sequences of amino acids with the capacity to recognize cholesterol. These cholesterol consensus domains have been designated as “CARC” and its mirror sequence “CRAC”. CARC preferentially occurs in the exoplasmic-facing membrane leaflet, and CRAC, in the cytoplasmic-facing hemilayer. Both motifs are highly conserved among ion-channel and neurotransmitter receptor proteins in vertebrate nervous systems, where they… More >

  • Open Access

    REVIEW

    Role of GM3 ganglioside in the pathology of some progressive human diseases and prognostic importance of serum anti-GM3 antibodies

    VERA KOLYOVSKA1,#, SONYA IVANOVA2, DESISLAVA DRENSKA3, DIMITAR MASLAROV3,4,#, RENETA TOSHKOVA1,*
    BIOCELL, Vol.45, No.6, pp. 1485-1494, 2021, DOI:10.32604/biocell.2021.016250
    (This article belongs to this Special Issue: Cellular Biomechanics in Health and Diseases)
    Abstract Glycosphingolipids (gangliosides) have been characterized as important biological molecules with a key role as regulators in many physiological processes on cellular, tissue, organ, and organism levels. The deviations in their normal amounts, production, and metabolism are very often related to the development of many multi-factor socially important diseases. GM3 ganglioside, as a small molecule, plays important roles in the cascade regulatory pathways in the pathology of many disorders like neurodegenerative diseases, autoimmune diseases, inflammation, diabetes, malignant transformation, and others. Ganglioside GM3 and its derivatives are membrane-bound glycosphingolipids composed of an oligosaccharide head structure containing one sialic acid residue. These molecules… More >

  • Open Access

    ARTICLE

    Viral integration detection strategies and a technical update on Virus-Clip

    DANIEL WAI-HUNG HO1,2,#, XUEYING LYU1,2,#, IRENE OI-LIN NG1,2,*
    BIOCELL, Vol.45, No.6, pp. 1495-1500, 2021, DOI:10.32604/biocell.2021.017227
    Abstract Oncovirus infection is crucial in human malignancies. Certain oncoviruses can lead to structural variations in the human genome known as viral genomic integration, which can contribute to tumorigenesis. Existing viral integration detection tools differ in their underlying algorithms pinpointing different aspects or features of viral integration phenomenon. We discuss about major procedures in performing viral integration detection. More importantly, we provide a technical update on Virus-Clip to facilitate its usage on the latest human genome builds (hg19 and hg38) and the adoption of multi-thread mode for faster initial read alignment. By comparing the execution of Virus-Clip using single-thread and multi-thread… More >

  • Open Access

    ARTICLE

    Targeted editing of intronic-splicing silencer enhancement of SMN2 Exon 7 inclusion by CRISPR/Case 9

    LIUCHENG WU1,3,#, YI WANG2,#, LILI DU1, GUIQING JI1, RUI ZHOU2, ZEYI ZHAO2, JUN CHEN2, SHUNXING ZHU1,*
    BIOCELL, Vol.45, No.6, pp. 1501-1507, 2021, DOI:10.32604/biocell.2021.09528
    Abstract Spinal muscular atrophy (SMA) is an autosomal recessive hereditary neuromuscular disease. Exon 7 and 8 of survival of motor neuron 1 (SMN1) gene or only exon 7 homology deletion leads to the failure to produce a full-length SMN gene. The copy number of SMN2 gene with high homology of SMN1 affects the degree of disease and was the target gene for targeting therapy, in which splicing silencer in intron 7 was the key to suppress the inclusion of exon 7. In this study, we projected to use CRISPR/Case 9 for the targeted editing of intronic-splicing silencer (ISS) sequence to promote… More >

  • Open Access

    REVIEW

    The signaling pathway in modulating bone metabolism after dental implant in diabetes

    XIAOMEI HAN#, SHUYING ZHANG#, YIFU WANG, CHANGE QI, PENGNYU GUO, YALI XU, GUANGHUI LYU*
    BIOCELL, Vol.45, No.6, pp. 1509-1519, 2021, DOI:10.32604/biocell.2021.09506
    Abstract Diabetes Mellitus is a systematic disease with complications in multi-organs, including decreased implant osseointegration and a high failure rate of dental transplants. Accumulating evidence indicates that the signaling pathway directly impacts the process of bone metabolism and inflammatory response implicated with dental implants in diabetic patients. This review summarizes the recent advance in signaling pathways regulate osseointegration and inflammatory response in dental transplantation, aiming to identify the potential therapeutic target to reduce the dental transplant failure in diabetes patients, with emphasis on the surface characteristics of the implant, inflammatory signaling, AMPK, PPARγ, WNT, ROS, and adiponectin signaling. More >

  • Open Access

    ARTICLE

    High level of circPTN promotes proliferation and stemness in gastric cancer

    KEWEI GAO1,#, JIANGFENG HU2,#, YI ZHOU3, LIANG ZHU1,*
    BIOCELL, Vol.45, No.6, pp. 1521-1526, 2021, DOI:10.32604/biocell.2021.09220
    Abstract Increasing evidence proves that circular RNAs (circRNAs) play an important role in regulating the biological behaviors of tumors. The central purpose of this research was to investigate the functions of circRNA in gastric cancer. The utilization of real-time PCR was to test circPTN expression in gastric cancer cells. Cell counting colony formation assays, CCK-8 assay, and EdU assay were used to investigate proliferation. Transwell assay was applied to investigate migration. We discovered that circPTN was highly expressed in gastric cancer cells. Low expression of circPTN inhibits gastric cancer cell proliferation and migration. Elevated expression of circPTN promotes gastric cancer cell… More >

  • Open Access

    ARTICLE

    Immune prognostic implications of PSMD14 and its associated genes signatures in hepatocellular carcinoma

    CHUAN TIAN1, MUBALAKE ABUDOUREYIMU1, XINRONG LIN1, HAO ZHOU2, XIAOYUAN CHU1, RUI WANG1,*
    BIOCELL, Vol.45, No.6, pp. 1527-1541, 2021, DOI:10.32604/biocell.2021.016203
    (This article belongs to this Special Issue: Decoding Gene (including circRNA, lincRNA miRNA and mRNA) Expression)
    Abstract PSMD14 played a vital role in initiation and progression of hepatocellular carcinoma (HCC). However, PSMD14 and its-related genes for the immune prognostic implications of HCC patients have rarely been analyzed. Messenger RNA expression profiles and clinicopathological data were downloaded from The Cancer Genome Atlas (TCGA) and International Cancer Genome Consortium (ICGC) database-Liver Hepatocellular Carcinoma (LIHC). Additionally, we used multi-dimensional bioinformatics analysis to construct and validate a PSMD14-based immune prognostic signature (including RBM45, PSMD1, OLA1, CCT6A, LCAT and IVD) for HCC prognosis prediction. Patients in the high-risk group shown significantly poorer survival than patients in the low-risk group. Calibration curves confirmed… More >

  • Open Access

    ARTICLE

    Basal cell carcinoma stem cells exhibit osteogenic and chondrogenic differentiation potential

    MAJA MILOSEVIC1,2, MILOS LAZAREVIC1,2, BOSKO TOLJIC1, MILAN PETROVIC2, MIROSLAV VUKADINOVIC2, ZORAN JEZDIC2, BOBAN ANICIC2, DRAGO JELOVAC2, SVETLANA JOVANOVIC3, JELENA MILASIN1,*
    BIOCELL, Vol.45, No.6, pp. 1543-1550, 2021, DOI:10.32604/biocell.2021.015060
    Abstract Specific cell subpopulations identified as cancer stem cells (CSCs) can be found in basal cell carcinoma (BCC). Generally, CSCs have a marked trans-differentiation potential that could potentially be used in differentiation therapies. However, there are no studies regarding BCC CSCs multipotency. The aim of the study was to analyze the characteristic of CSCs of BCC with emphasis on their differentiation potential upon specific induction. Specific staining and cell morphology were used for differentiation confirmation, along with the expression analysis of osteogenic (ALP, BSP, Runx2, OCN, BMP2), chondrogenic (COL1 and COL2A1), adipogenic (PPAR-γ) and neurogenic (Nestin and MAP2) markers. BCC CSCs… More >

  • Open Access

    ARTICLE

    Astragaloside IV improves melanocyte differentiation from mouse bone marrow mesenchymal stem cells

    XINGYU MEI, ZHOUWEI WU, CHENGZHONG ZHANG, YUE SUN, WEIMIN SHI*
    BIOCELL, Vol.45, No.6, pp. 1551-1559, 2021, DOI:10.32604/biocell.2021.015376
    Abstract Vitiligo results in an autoimmune disorder destructing skin pigment cells, melanocytes (Mcs). This study aimed to investigate whether Astragaloside IV (AIV) could efficiently induce differentiation of bone marrow mesenchymal stem cells (BMMSCs) into Mcs. BMMSCs were induced and differentiated into Mcs with 0.1, 0.2, and 0.4 mg/L AIV during 150-day. Morphologic changes of differentiated cells were observed. Levels of some melanocytic specific genes (TRP-1, TRP-2, MART-1, Mitf) were measured with quantitative polymerase chain reaction (qPCR) at 90, 120, and 150 days of induction. After 90-day induction, the differentiated cells with 0.4 mg/L AIV demonstrated the typical morphology of Mcs, positive… More >

  • Open Access

    ARTICLE

    Investigation of the antioxidant defensive role of both AD-MSCs and BM-MSCs in modulating the alteration in the oxidative stress status in various STZ-diabetic rats’ tissues

    SHADY G. EL-SAWAH1,*, FAYEZ ALTHOBAITI2, ADIL ALDHAHRANI3, EMAN FAYAD2, MARWA A. ABDEL-DAYEM4, REHAB M. AMEN5, EL SHAIMAA SHABANA6, EHAB I. EL-HALLOUS7, HANAN M. RASHWAN1
    BIOCELL, Vol.45, No.6, pp. 1561-1568, 2021, DOI:10.32604/biocell.2021.016869
    (This article belongs to this Special Issue: Cell-Based Regenerative Therapies)
    Abstract Diabetes mellitus (DM) could negatively affect patients’ health via inducing a lot of serious functional hazards in many tissues’ cells at molecular levels. Recently, many scientists had proposed stem cell therapy being an appropriate alternative treatment protocol for numerous health threatening issues including diabetes. Therefore, the current study was designed to investigate the antioxidant potentiality of two MSCs types in alleviating tissues’ oxidative stress dramatic elevation resulting as a consequence of Type 1 DM induction. In our 4 weeks study, animals were divided into four groups: control group, STZ-diabetic group (D), D+AD-MSCs group and D+BM-MSCs group. Data reported that diabetic… More >

  • Open Access

    ARTICLE

    Repurposing of FDA-Approved drugs to predict new inhibitors against key regulatory genes in Mycobacterium tuberculosis

    XINJUN YANG1, AFTAB ALAM2, NAIYAR IQBAL3, KHALID RAZA4,*
    BIOCELL, Vol.45, No.6, pp. 1569-1583, 2021, DOI:10.32604/biocell.2021.017019
    Abstract

    Tuberculosis (TB) disease has become one of the major public health concerns globally, especially in developing countries. Numerous research studies have already been carried out for TB, but we are still struggling for a complete and quick cure for it. The progress of Mycobacterium tuberculosis (MTB) strains resistant to existing drugs makes its cure and control very complicated. Therefore, it is the need of the hour to search for newer and effective drugs that can inhibit an increasing number of putative drug targets. We applied the drug repurposing concept to identify promising FDA-approved drugs against five key-regulatory genes (FurB, IdeR,… More >

  • Open Access

    ARTICLE

    Propofol inhibits cells migration and invasion via HOTAIR/miR-93/HIF-1α-mediated lactate secretion in colon cancer

    RUONAN GU1,2, WENJING GUO2, WENYANG WANG1, GUIHUAN LI1, XIAOJU LAI1, ZHIBIN HUANG1, WANLU ZHAO1, ZHICONG WU1, HUA CHEN1, WENYANG LUO1, FANGYIN ZENG3,*, FAN DENG1,*
    BIOCELL, Vol.45, No.6, pp. 1585-1599, 2021, DOI:10.32604/biocell.2021.017016
    (This article belongs to this Special Issue: Regulatory, Therapeutic and Diagnostic Associations of miRNA in Emerging Diseases)
    Abstract Propofol, a common intravenous anesthetic used in clinical, has been shown to regulate cells proliferation, inflammation, angiogenesis and metastasis as well as exerting anti-cancer effect in several different types of cancer. However, the functions and mechanisms of propofol in lactate secretion and invasion of cancer cells remain unknown. The primary aim of this study was to investigate the role of propofol in cells migration and invasion of colon cancer (CRC). Scratches assay, Transwell assay were used to detect colon cells migration and invasion. Real-time Quantitative Polymerase Chain Reaction (RT-qPCR), Western blotting were utilized to detect the expression of related molecules.… More >

  • Open Access

    ARTICLE

    Identification of potential inhibitors for Sterol C-24 reductase of Leishmania donovani through virtual screening of natural compounds

    FAZLUR RAHMAN1,#, SHAMS TABREZ1,#, RAHAT ALI1, SAJJADUL KADIR AKAND1, MOHAMMED A. ALAIDAROUS2,3, MOHAMMED ALSAWEED2, BADER MOHAMMED ALSHEHRI2, SAEED BANAWAS2,3, ABDUR RUB1,*, ABDUL AZIZ BIN DUKHYIL2,*
    BIOCELL, Vol.45, No.6, pp. 1601-1610, 2021, DOI:10.32604/biocell.2021.016682
    Abstract Leishmaniasis is a vector-borne parasitic neglected tropical disease caused by a group of about 30 different species of the genus Leishmania. It is transmitted by the bite of female phlebotomies sand fly. Three main clinical manifestations of leishmaniasis include cutaneous, visceral, and mucocutaneous leishmaniasis. Visceral leishmaniasis (VL) caused by Leishmania donovani, is an infection of reticuloendothelial system and fatal if untreated. Cholesterol, a sterol that is prominent in the mammalian cell membranes whereas stigmasterol and ergosterol are more prevalent in plants, yeast, and protozoa, respectively. Ergosterols which is absent in human being, is an important constituent of parasite membrane. Sterol… More >

  • Open Access

    ARTICLE

    RNA-sequencing indicates high hemocyanin expression as a key strategy for cold adaptation in the Antarctic amphipod Eusirus cf. giganteus clade g3

    SAMUELE GRECO1,#, ELISA D’AGOSTINO2,#, CHIARA MANFRIN1, ANASTASIA SERENA GAETANO1, GAEL FURLANIS1, FRANCESCA CAPANNI1, GIANFRANCO SANTOVITO2, PAOLO EDOMI1, PIERO GIULIO GIULIANINI1, MARCO GERDOL1,*
    BIOCELL, Vol.45, No.6, pp. 1611-1619, 2021, DOI:10.32604/biocell.2021.016121
    Abstract We here report the de novo transcriptome assembly and functional annotation of Eusirus cf. giganteus clade g3, providing the first database of expressed sequences from this giant Antarctic amphipod. RNA-sequencing, carried out on the whole body of a single juvenile individual likely undergoing molting, revealed the dominant expression of hemocyanins. The mRNAs encoding these oxygen-binding proteins cumulatively accounted for about 40% of the total transcriptional effort, highlighting the key biological importance of high hemocyanin production in this Antarctic amphipod species. We speculate that this observation may mirror a strategy previously described in Antarctic cephalopods, which compensates for the decreased ability… More >

  • Open Access

    ARTICLE

    Oxidative stress indicators in human and bottlenose dolphin leukocytes in response to a pro-inflammatory challenge

    TARYN E. SYMON1, RAMÓN GAXIOLA-ROBLES1,2, CLAUDIA J. HERNÁNDEZ-CAMACHO3, TANIA ZENTENO-SAVÍN1,*
    BIOCELL, Vol.45, No.6, pp. 1621-1630, 2021, DOI:10.32604/biocell.2021.016302
    (This article belongs to this Special Issue: Oxidative Stress in Aquatic Organisms)
    Abstract Marine mammals undergo cycles of tissue ischemia and reperfusion during the dive response. Reperfusion injury can result in oxidative tissue damage and the activation of a pro-inflammatory immune response. The risk of oxidative damage is reduced by antioxidants. Our hypothesis is that the reported higher antioxidant defenses within marine mammal tissues provide additional protection in situations that produce oxidative stress, like inflammation, in comparison to terrestrial mammal tissues. Leukocytes were isolated from the whole blood of Pacific bottlenose dolphins (Tursiops truncatus gilli) and humans (Homo sapiens) and were exposed to lipopolysaccharides (LPS, 10 µg/mL) in vitro to simulate a pro-inflammatory… More >

  • Open Access

    ARTICLE

    Application of ferrous sulfate alleviates negative impact of cadmium in rice (Oryza sativa L.)

    JAVARIA AFZAL1,2,#, XIUKANG WANG3,*, MUHAMMAD HAMZAH SALEEM4,#, XUECHENG SUN1, SHAHID HUSSAIN5, IMRAN KHAN6, MUHAMMAD SHOAIB RANA1, SHAKEEL AHMED7, SAMRAH AFZAL AWAN6, SAJID FIAZ8, OMAR AZIZ9, KASHIF ALI KUBAR10, SHAFAQAT ALI11,12, CHENGXIAO HU1,*
    BIOCELL, Vol.45, No.6, pp. 1631-1649, 2021, DOI:10.32604/biocell.2021.014934
    Abstract Soil contamination with toxic heavy metals [such as cadmium (Cd)] is becoming a serious global problem due to rapid development of social economy. Iron (Fe), being an important element, has been found effective in enhancing plant tolerance against biotic and abiotic stresses. The present study investigated the extent to which different levels of Ferrous sulphate (FeSO4) modulated the Cd tolerance of rice (Oryza sativa L.), when maintained in artificially Cd spiked regimes. A pot experiment was conducted under controlled conditions for 146 days, by using natural soil, mixed with different levels of CdCl2 [0 (no Cd), 0.5 and 1 mg/kg]… More >

  • Open Access

    ARTICLE

    Doubled haploid production in advanced back cross generations and molecular cytogenetic characterization of rye chromatin in triticale × wheat derived doubled haploid lines

    MUNIYANDI SAMUEL JEBERSON1,*, HARINDER KUMAR CHAUDHARY2, RAKESH KUMAR CHAHOTA3, SHABIR HUSSAIN WANI4,*
    BIOCELL, Vol.45, No.6, pp. 1651-1659, 2021, DOI:10.32604/biocell.2021.015735
    Abstract The rye genome has shown potential for improvement of bread wheat, where wheat-rye substitutions and translocations have been and are frequently used in resistance breeding. Crosses belongs to different generations viz., BC1F1, BC1F2, BC1F3, BC1F4 and BC2F3 of triticale × wheat derived were used for different haploid induction parameters using Gogon grass (Imperata cylindrica) as a pollen source. The percentage of pseudo seed formation ranged from 34.55% for BC1F2 to 63.77 for BC1F1 crosses, the haploid embryo formation ranges from 9.43% for BC1F1 to 30.2% for BC1F2, the haploid plant generation ranges from 19.36% for BC1F2 to 63.25% for BC1F1.… More >

  • Open Access

    ARTICLE

    Biosynthesis of raw starch degrading β-cyclodextrin glycosyltransferase by immobilized cells of Bacillus licheniformis using potato wastewater

    YASSER S. MOSTAFA1,*, SAAD A. ALAMRI1,2, SULIMAN A. ALRUMMAN1, TAREK H. TAHA3, MOHAMED HASHEM1,4, MAHMOUD MOUSTAFA1,5, LAMIAA I. FAHMY6
    BIOCELL, Vol.45, No.6, pp. 1661-1672, 2021, DOI:10.32604/biocell.2021.016193
    Abstract The study was sought to enhance the synthesis of thermal stable β-cyclodextrin glycosyltransferase (β-CGTase) using potato wastewater as a low-cost medium and assess the degree to which it is efficient for industrial production of β-cyclodextrin (β-CD) from raw potato starch. Thermophilic bacteria producing β-CGTase was isolated from Saudi Arabia and the promising strain was identified as Bacillus licheniformis using phylogenetic analysis of the 16S rRNA gene. Alginate-encapsulated cultures exhibited twice-fold of β-CGTase production more than free cells. Scanning electron microscopy (SEM) of polymeric capsules indicated the potential for a longer shelf-life, which promotes the restoration of activity in bacterial cells… More >

  • Open Access

    ARTICLE

    Genome-wide identification and in silico gene expression analysis of the related to ABI3/VP1 (RAV) transcription factor family in barley (Hordeum vulgare L.)

    CÜNEYT UÇARLI*
    BIOCELL, Vol.45, No.6, pp. 1673-1685, 2021, DOI:10.32604/biocell.2021.016316
    Abstract RAV (Related to ABI3/VP1) transcription factors are unique members of the AP2-ERF superfamily with AP2 and B3 domains and play important roles in the regulation of seed germination, plant growth, and stress response. In the study, 7 RAV genes, named HvRAVs, were identified in barley based on the available genome sequences. While five of the seven HvRAVs were located on chromosome 3, HvRAV5 and HvRAV7 were located on chromosome 1 and 4, respectively. Six of the predicted HvRAVs were intron-less, except HvRAV2, which had one intron. HvRAV proteins have shown basic, instable, and hydrophilic properties. The AP2 domain specific RAYD… More >

  • Open Access

    ARTICLE

    Identification of PtGai (a DELLA protein) in trifoliate orange and expression patterns in response to drought stress

    XIAOFEN CHENG1, ABEER HASHEM2,3, ELSAYED FATHI ABD_ALLAH4, QIANGSHENG WU1,5,*, KAMIL KUČA5,*
    BIOCELL, Vol.45, No.6, pp. 1687-1694, 2021, DOI:10.32604/biocell.2021.017581
    Abstract Gibberellins (GAs) are an important hormone in regulating plant growth and development, and DELLA protein is an essential negative regulator of GA signal transduction. The aim of the study was to clone a GA-inhibiting protein DELLA from trifoliate orange (Poncirus trifoliata L. Raf.) and to analyze the bioinformations and expression patterns of the protein gene in tissues and in response to drought stress. A DELLA protein was isolated from trifoliate orange and named as PtGai (Genebank number: MZ170959). The PtGai protein had 1731 bp open reading frames, along with 576 amino acid codes, and also grouped with sweet orange (XM_006430552.4).… More >

  • Open Access

    ARTICLE

    Complete genome sequence of two strawberry vein banding virus isolates from China

    LEI JIANG1,2,3,#, SHUAI LI1,#, XIZI JIANG1, TONG JIANG1,2,3,*
    BIOCELL, Vol.45, No.6, pp. 1695-1702, 2021, DOI:10.32604/biocell.2021.015250
    Abstract It was rarely reported about strawberry vein banding virus (SVBV) genome sequence in China and most countries worldwide. In this work, we determined the complete genome sequences of two SVBV isolates in China, designated SVBV-AH and SVBV-BJ, that were obtained from naturally infected strawberry samples from Anhui province and Beijing city of China, respectively. The complete genomes of SVBV-AH and SVBV-BJ were 7,862 nucleotides (nts) and 7,863 nts long, respectively, and both constituted with seven genes typical of the caulimoviruses. Alignment of complete nucleotide sequences showed that SVBV-AH and SVBV-BJ shared a significant nucleotide sequence identity of 97.7% of each… More >

Share Link

WeChat scan