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High level of circPTN promotes proliferation and stemness in gastric cancer

KEWEI GAO1,#, JIANGFENG HU2,#, YI ZHOU3, LIANG ZHU1,*

1 Department of Gastroenterology, Shanghai Changzheng Hospital, The Second Military Medical University, Shanghai, 200001, China
2 Department of Gastroenterology, Shanghai First People’s Hospital, Shanghai, 200003, China
3 Department of Gastroenterology, Shanghai Hospital of Integrated Traditional Chinese and Western Medicine, Shanghai, 200003, China

* Corresponding Author: LIANG ZHU. Email: email
# Contributing equally to this work

BIOCELL 2021, 45(6), 1521-1526. https://doi.org/10.32604/biocell.2021.09220

Abstract

Increasing evidence proves that circular RNAs (circRNAs) play an important role in regulating the biological behaviors of tumors. The central purpose of this research was to investigate the functions of circRNA in gastric cancer. The utilization of real-time PCR was to test circPTN expression in gastric cancer cells. Cell counting colony formation assays, CCK-8 assay, and EdU assay were used to investigate proliferation. Transwell assay was applied to investigate migration. We discovered that circPTN was highly expressed in gastric cancer cells. Low expression of circPTN inhibits gastric cancer cell proliferation and migration. Elevated expression of circPTN promotes gastric cancer cell proliferation and migration. Moreover, we discovered that circPTN could accelerate self-renewal and increase the expression of stemness markers. The results of our study suggested that a high level of circPTN expression promotes the proliferation and stemness of gastric cancer cells.

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APA Style
GAO, K., HU, J., ZHOU, Y., ZHU, L. (2021). High level of circptn promotes proliferation and stemness in gastric cancer. BIOCELL, 45(6), 1521-1526. https://doi.org/10.32604/biocell.2021.09220
Vancouver Style
GAO K, HU J, ZHOU Y, ZHU L. High level of circptn promotes proliferation and stemness in gastric cancer. BIOCELL . 2021;45(6):1521-1526 https://doi.org/10.32604/biocell.2021.09220
IEEE Style
K. GAO, J. HU, Y. ZHOU, and L. ZHU "High level of circPTN promotes proliferation and stemness in gastric cancer," BIOCELL , vol. 45, no. 6, pp. 1521-1526. 2021. https://doi.org/10.32604/biocell.2021.09220



cc This work is licensed under a Creative Commons Attribution 4.0 International License , which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
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