Home / Journals / BIOCELL / Vol.26, No.2, 2002
Table of Content
  • Open Access

    REVIEW

    Review : Reticulocyte maturation: mitoptosis and exosome release

    CHARLES GÉMINARD, AUDE DE GASSART, MICHEL VIDAL
    BIOCELL, Vol.26, No.2, pp. 205-215, 2002, DOI:10.32604/biocell.2002.26.205
    Abstract During the differentiation of erythroid cells, a vast program of maturation takes place, leading to decay or elimination of organelles, including the nucleus, mitochondria, ribosomes, lysosomes, endoplasmic reticulum and Golgi apparatus. During the last step of red cell maturation, remaining organelles, primarily mitochondria and ribosomes but also vestiges of others are finally cleared from the cell. This cleaning session also affects specific proteins that are partially or entirely removed from the cell surface. The interplay of the various events and their causal relationships are approached here. More >

  • Open Access

    ARTICLE

    Transgenic wheat plants resistant to herbicide BASTA obtained by microprojectile bombardment

    MARIANA N. MELCHIORRE, HERNÁN R. LASCANO, VICTORIO S.TRIPPI
    BIOCELL, Vol.26, No.2, pp. 217-223, 2002, DOI:10.32604/biocell.2002.26.217
    Abstract Wheat (Triticum aestivum) transgenic plants of an important commercial cv (Oasis) was obtained with an efficient and short procedure. First, the optimum conditions for the embriogenic calli generation from inmature embryos with a high regeneration percentage were established. The transformation of calli was performed by high velocity microprojectile bombardment, using the pAHC25 plasmid, which contains the reporter gene β-glucuronidase (GUS) and the selectable BAR gene which confers resistance to the herbicide Basta. The transformations were confirmed by β-glucuronidase assay activity, PCR and Southern blot analysis. The efficiency of this procedure was high and similar to other reports in which “model”… More >

  • Open Access

    ARTICLE

    Ciprofloxacin increases hepatic and renal lipid hydroperoxides levels in mice

    ALICIA I. WEYERS, LAURA I. UGNIA, HUGO GARCÍA OVANDO, NORA B. GORLA*
    BIOCELL, Vol.26, No.2, pp. 225-228, 2002, DOI:10.32604/biocell.2002.26.225
    Abstract Ciprofloxacin (CFX) is an effective and relatively safe antimicrobial used in a variety of human infections. However, adverse drug reactions and positive results in genotoxic tests are reported.
    In order to understand the possible pathophysiological mechanisms of the toxic effects informed for CFX, lipid hydroperoxides (LOOH) -oxidative mediators of peroxidation- were quantified in liver and kidney of mice, after 15 to 360 minutes of the ciprofloxacin administration at doses of 10 mg/ Kg or 100 mg/ Kg by ip route. The peroxidation in the lipid fraction was evaluated by measuring the amount of hydroperoxides through the oxidation of 1-… More >

  • Open Access

    ARTICLE

    Ultrastructure of the ovariole sheath in Diatraea saccharalis (Lepidoptera: Pyralidae)

    DANIELA CARVALHO DOS SANTOS, ELISA APARECIDA GREGÓRIO
    BIOCELL, Vol.26, No.2, pp. 229-235, 2002, DOI:10.32604/biocell.2002.26.229
    Abstract The ultrastructure of the ovariole sheath along the Diatraea saccharalis ovariole was studied by scanning and transmission electron microscopy. Each ovariole is surrounded by an epithelial sheath, a tunica propria and scattered lumen cells. These three components of the ovariole sheath show different ultrastructural features along the ovariole, in the germarium or in the vitellarium; these differences are more evident in the epithelial sheath cells. The epithelial sheath is composed by two layers of cells, the external one running longitudinally and the internal one running circularly in the ovariole. These cells, in vitellarium, present cytoplasmic bundles of myofilaments that are… More >

  • Open Access

    ARTICLE

    Effect of the Lipophilic o-Naphthoquinone CG 10-248 on Rat Liver Mitochondria Structure and Function

    LIDIA M. LOPEZ*, AMANDA PELLEGRINO DE IRALDI*, PATRICIA H. CARRIZO**, MARTA DUBIN**, ANDRÉS O.M. STOPPANI**
    BIOCELL, Vol.26, No.2, pp. 237-245, 2002, DOI:10.32604/biocell.2002.26.237
    Abstract CG 10-248 (3,4-dihydro-2,2 dimethyl-9-chloro-2H-naphtho[1,2b]pyran-5,6-dione), a ß-lapachone analogue, modified the ultrastructure of rat liver mitochondria in vitro, in the absence of added oxidizable substrates. The condensed mitochondrial state was replaced by the orthodox or swollen state to a significant degree. The number of modified mitochondria depended on incubation time and quinone concentration, in the 25-100 µM range. Under the same experimental conditions, mitochondrial respiration was uncoupled as indicated by the increase in the rate of succinate oxidation by controlled mitochondria in metabolic state “4” (not in state “3”), and by the activation of latent F0 F1 -ATP synthase. Taking into account… More >

  • Open Access

    ARTICLE

    Distribution of NADPH-diaphorase in rat mesencephalon: A light and electron microscopical study

    JUAN J. LÓPEZ-COSTA, CAROLINA ACUIPIL, PATRICIA TAGLIAFERRO, SAMEER PATEL, JAVIER RAMOS, ALICIA BRUSCO, JORGE PECCI SAAVEDRA*
    BIOCELL, Vol.26, No.2, pp. 247-252, 2002, DOI:10.32604/biocell.2002.26.247
    Abstract NADPH-diaphorase is a useful technique to reveal NO producing neurons at light microscopic level (LM). A modification of the technique using the tetrazolium salt BSPT as susbtrate, is useful to study the ultrastructure of NO neurons. The aim of this work was to perform a detailed analysis of NADPH diaphorase reactive neurons in rat mesencephalon both at light and electron microscopic levels.
    NADPH-diaphorase reactive neurons were observed in superior colliculus, in central gray matter, in dorsal and medial raphe and in the pedunculopontine tegmental nucleus using two histochemical techniques at LM. Electron microscopy showed deposits on membranes of the… More >

  • Open Access

    ARTICLE

    Ultrastructural and cytochemical changes in the liver of primary biliary cirrhosis patients

    ARTURO D. JORGE*, LUIS S. GUTIERREZ**, OLIVER JORGE*, MARIO H. BURGOS**
    BIOCELL, Vol.26, No.2, pp. 253-262, 2002, DOI:10.32604/biocell.2002.26.253
    Abstract The aim of this paper is to establish whether there are cytochemical or ultrastructural alterations in the hepatocytes of patients with primary biliary cirrhosis (PBC) at stages I and II compared with the biopsies from individuals with normal liver. Cytochemical technique with ATP as substrate, transmission electron microscopy (TEM) and freeze fracture were used for the studies.
    In the normal liver biopsies the ultrastructural cytochemical localization of the enzymatic activity was clearly shown in the bile canaliculi. In the PBC biopsies, the enzymatic activity is increased in the bile canaliculi and is also present in the lateral membranes of… More >

  • Open Access

    ARTICLE

    Brief Note : Improved in vitro embryo development of stenospermic grape by putrescine

    MARÍA TERESA PONCE, MÓNICA ELIZABETH GUIÑAZÚ, RICARDO TIZIO
    BIOCELL, Vol.26, No.2, pp. 263-266, 2002, DOI:10.32604/biocell.2002.26.263
    Abstract The goal of this study was to determine the effect of putrescine, added to the culture medium, on the in vitro development of stenospermic grape (Vitis vinifera L) embryos. The cross breedings of Perlón x G.C88552 and Perlón x Argentina were used. 0 (control), 2 and 4 mM of putrescine were added to the immature seed’s culture medium. In Perlón x Argentina, 2mM of putrescine statistically increased the percentage of total embryos, direct germination, polyembryos and normal plants. In Perlón x G.C88552, only 2 mM of putrescine increased all the variables considered, eventually tripling the percentage of normal plants obtained.… More >

Share Link

WeChat scan