Open Access

ARTICLE

Brief Note : Improved in vitro embryo development of stenospermic grape by putrescine

MARÍA TERESA PONCE, MÓNICA ELIZABETH GUIÑAZÚ, RICARDO TIZIO

Departamento de Biología. Cátedra de Fisiología Vegetal. Facultad de Ciencias Agrarias. Universidad Nacional de Cuyo. Mendoza, Argentina.
* Prof. Eng. Ricardo M. Tizio died on April 16^th, 2002.

Corresponding Author: María Teresa Ponce, Tel.: (+54-261) 496 0431; Fax: (+54-261) 496 0469; E-mail:

BIOCELL 2002, 26(2), 263-266. https://doi.org/10.32604/biocell.2002.26.263

Abstract

The goal of this study was to determine the effect of putrescine, added to the culture medium, on the in vitro development of stenospermic grape (Vitis vinifera L) embryos. The cross breedings of Perlón x G.C88552 and Perlón x Argentina were used. 0 (control), 2 and 4 mM of putrescine were added to the immature seed’s culture medium. In Perlón x Argentina, 2mM of putrescine statistically increased the percentage of total embryos, direct germination, polyembryos and normal plants. In Perlón x G.C88552, only 2 mM of putrescine increased all the variables considered, eventually tripling the percentage of normal plants obtained. The results suggest that the endogenous concentration of putrescine may be a growth limiting factor. Adding putrescine to the culture medium of immature grape seeds is a legitimate resource to significantly increase the results of this technique.

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