Home / Journals / BIOCELL / Vol.30, No.2, 2006
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  • Open Access

    ARTICLE

    Determination of vanadium accumulation in onion root cells (Allium cepa L.) and its correlation with toxicity

    LETTY MARCANO, INGRID CARRUYO, YUSMARY FERNÁNDEZ, XIOMARA MONTIEL, ZAIDA TORREALBA.
    BIOCELL, Vol.30, No.2, pp. 259-267, 2006, DOI:10.32604/biocell.2006.30.259
    Abstract The vanadium is a metal that presents great interest from the toxicological point of view, because of the numerous alterations that can take place in different biological systems. This work evaluated the capacity of vanadium accumulation and its correlation with genotoxic effects in root cells of Allium cepa L. The bulbs were cultivated in renovated filtered water each 24 h, at a temperature of 25 ± 0.5°C, in darkness and constant aeration. Treatments were carried out under the same experimental conditions, using water solutions of vanadium of 25, 50, 75 and 100μg/g for 0, 12, 24, 48 and 72 h.… More >

  • Open Access

    ARTICLE

    Construction of engineered murine Embryonic stem cells with conditional knockout of FGFR2 depending on Cre-loxP

    WANG JIANMIN, SONG RUIHUA, CHEN LEI, YIN LIANGJUN, CHEN BO, SUN JING, GOU YUANBING, ZHAO LING, CHEN LIN
    BIOCELL, Vol.30, No.2, pp. 269-278, 2006, DOI:10.32604/biocell.2006.30.269
    Abstract Objective: To investigate the functions of Fibroblast Growth Factor Receptor-2 (FGFR2) at different stages of cell differentiation. The engineered murine embryonic stem (ES) cells with conditional knockout of FGFR2 were developed depending on Cre-loxP. Methods: Cre-loxP system was used in a conditional targeting vector. The competent AM-1 bacteria, which expressed Cre-recombinase, was used to confirm the Cre-mediated deletion of the floxed exons 7 and 8 of FGFR2. The targeting vector was electroporated into the ES cells, and the transfected ES cells were screened with G418 and Ganciclovir. Finally, the ES clones with correct targeting events were identified by Southern Blot… More >

  • Open Access

    ARTICLE

    Comparative fine structure of the epididymal spermatozoa from three korean shrews with considerations on their phylogenetic relationships

    SOON-JEONG JEONG1, JOO-CHEOL PARK1, HEUNG-JOONG KIM2, CHUN SIK BAE3, MYUNG-HEE YOON4, DO-SEON LIM5, MOON-JIN JEONG1
    BIOCELL, Vol.30, No.2, pp. 279-286, 2006, DOI:10.32604/biocell.2006.30.279
    Abstract This study examined the fine structures of epididymal spermatozoa on the lesser white-toothed shrew (Crocidura suaveolens), the Japanese white-toothed shrew (C. dsinezumi) and the big white-toothed shrew (C. lasiura) belonging to the subfamily Crocidurinae living in Korea.
    In the spermatozoa of C. suaveolens, the head has a large acrosome, a smooth inner acrosomal membrane and a wavy, finger-like, electron-dense apical body. The neck has a solid proximal centriole that is filled with electron-dense material. These results showed the spermatozoa of C. suaveolens possess the characteristics of both Crocidurinae and Soricinae. In C. dsinezumi and C. lasiura, the head has… More >

  • Open Access

    ARTICLE

    Morphology and histology of P. argentinus (Crustacea, Decapoda, Caridea) digestive tract

    LILIANA SOUSA1, ANA MARÍA PETRIELLA1,2
    BIOCELL, Vol.30, No.2, pp. 287-294, 2006, DOI:10.32604/biocell.2006.30.287
    Abstract This work describes the morphology and histology of the P. argentinus digestive tract. The foregut comprises the mouth, oesophagus, and stomach and is lined by a simple cylindrical epithelium overlain by cuticle. There are tegumental glands in the oral region and in the first portion of the oesophagus and of the hindgut. The cardiac stomach is an oval dorsal sac in the cephalothorax and has no calcified structures. The pyloric stomach comprises an upper chamber and a lower gland filter. The filter consists of an outer row of elongated setae and an inner row of dorsally curved setae forming longitudinal… More >

  • Open Access

    ARTICLE

    Effects of the ascorbic acid supplementation on NADH-diaphorase myenteric neurons in the duodenum of diabetic rats

    MARLI APARECIDA DOS SANTOS PEREIRA, MARIA CLAÚDIA BAGATIN, JACQUELINE NELISIS ZANONI
    BIOCELL, Vol.30, No.2, pp. 295-300, 2006, DOI:10.32604/biocell.2006.30.295
    Abstract We assessed the ascorbic acid (AA) supplementation on the myenteric neurons in the duodenum of rats. Fifteen rats with 90 days of age were divided into three groups: control (C), diabetics (D) and ascorbic acid treated diabetics (DA). After 120 days of daily treatment with AA, the duodenum was submitted to the NADH-diaphorase (NADH-d) histochemical technique, which allowed us to evaluate the neuronal density in an area of 8.96 mm2 for each duodenum, and also to measure the cellular profile area of 500 neurons per group. The supplementation promoted an increase on AA levels. The neuronal density (p <0.05) was… More >

  • Open Access

    ARTICLE

    Evaluation of a multiplex PCR method to detect enteroaggregative Escherichia coli

    M.E. RÜTTLER, C.S. YANZÓN, M.J. CUITIÑO, N.F. RENNA, M.A. PIZARRO, A.M. ORTIZ.
    BIOCELL, Vol.30, No.2, pp. 301-308, 2006, DOI:10.32604/biocell.2006.30.301
    Abstract Enteroaggregative Escherichia coli (EAEC) has been implicated in sporadic diarrhea in children and adults and has been identified as the cause of several outbreaks worldwide. The HEp-2 test remains the gold standard for identification of this pathotype. A 60-65 MDa plasmid encodes the aggregative adherence fimbriae (AAF/I and AAF/II), a transcriptional activator (aggR gene), the enteroaggregative heat-stable enterotoxin EAST1 (astA gene) and a cytotoxin (Pet). The standard assay for EAEC is performed only in research laboratories, because it is expensive, labor intensive and time-consuming. The Polymerase Chain Reaction (PCR) offers the possibility of rapid diagnosis. In the current study, a… More >

  • Open Access

    ARTICLE

    CAS role in the brain apoptosis of Bufo arenarum induced by cypermethrin

    M. F. IZAGUIRRE, M. N. VERGARA, V. H. CASCO.
    BIOCELL, Vol.30, No.2, pp. 309-320, 2006, DOI:10.32604/biocell.2006.30.309
    Abstract CAS might have a key role in the apoptosis induced by toxins, acting as anti-apoptotic factor, stimulating the cellular proliferation and the cell contact stabilization. To start to elucidate their role in the brain apoptosis of Bufo arenarum induced by cypermethrin (CY), the expression patterns of CAS and several cell adhesion molecules (CAMs) were established.
    Bufo arenarum tadpoles of the control and acute bioassay survival at different doses (39, 156, 625 and 2,500 μg CY/L) and times (24, 48, 72 and 96 h) of CY treatment were fixed in Carnoy, embedded in paraffin and sectioned. CAS and CAMs expression… More >

  • Open Access

    ARTICLE

    Brief Note : Salicylic acid permeation: A comparative study with different vehicles and membranes

    MÓNICA S. OLIVELLA, NORA B. DEBATTISTA, NORA B. PAPPANO
    BIOCELL, Vol.30, No.2, pp. 321-324, 2006, DOI:10.32604/biocell.2006.30.321
    Abstract Considering the skin´s function, different dermal pharmaceutical forms can be developed according to the type of therapeutic activity, active principle and excipients involved in the formulation, such as “transdermal preparations”. In the present study, the permeation parameters of the non-steroidal antiinflammatory drug, salicylic acid (SA) through synthetic membrane, polyvinyliden difluoride, and a biological membrane, egg shell membrane, with different vehicles, propylene glycol, isopropyl alcohol and carbopol gel, were determined. The reported physicochemical parameters of SA from CG were significantly higher than those obtained using PG and IP. This is attributed to the lipophilic nature of the vehicle that facilitates the… More >

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