Home / Journals / BIOCELL / Vol.46, No.11, 2022
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Bacterium reconstructs its septal peptidoglycan cell wall (PG) during cell division. This process is precisely regulated in time and space to separate the daughter cells’ chromosomes and maintain the shape of new cell poles. The cytoskeletal protein FtsZ forms a ring-like structure at the division site with treadmilling behavior driven by the energy from GTP hydrolysis. In the current viewpoint, we summarized recent studies focusing on the dynamics of FtsZ and PG synthases. These new results suggested that FtsZ serves as a train that shuttles PG synthases (such as FtsW) to proper division sites and regulates their activity.

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  • Open Access

    VIEWPOINT

    How does FtsZ’s treadmilling help bacterial cells divide?

    XINXING YANG*, RUIJIAO LIU
    BIOCELL, Vol.46, No.11, pp. 2343-2351, 2022, DOI:10.32604/biocell.2022.022100
    Abstract Most bacteria assemble a ring-like macromolecular machinery scaffolded by the essential cytoskeletal protein FtsZ for cell division. Studies have broadly explored how FtsZ could polymerize at the correct place and time. Recently, the FtsZ-ring was found to exhibit dynamic treadmilling along the circumference of the division site, driven by GTP hydrolysis. This apparently directional motion of FtsZ seems to drive the movement of septal cell wall synthesis enzymes and to play an important role in modulating cell envelope constriction and septum morphogenesis. However, the relationship between FtsZ’s treadmilling dynamics and cell wall synthesis varies in different bacteria. More importantly, the… More >

  • Open Access

    VIEWPOINT

    Ready to migrate? Reading cellular signs of migration in an epithelial to mesenchymal transition model

    TAMARA FERNÁNDEZ-CALERO1,2,3,#, IGNACIO LÓPEZ1,#, MARCOS DAVYT1, CORA CHALAR1, RICARDO EHRLICH1,4, MÓNICA MARÍN1,*
    BIOCELL, Vol.46, No.11, pp. 2353-2356, 2022, DOI:10.32604/biocell.2022.020966
    (This article belongs to this Special Issue: Tumor Microenvironment and Cytoskeletal Dynamics)
    Abstract The epithelial to mesenchymal transition (EMT) is a cellular program that drives de-differentiation of cells in both physiological and pathological processes. One of the characteristics of cells describing an EMT is the (re)acquisition of a motility capacity that allows them to migrate through the original tissue as well as to other sites in the organism. The molecular mechanisms that control the EMT are rapidly emerging and here we add to the idea that the adaptation required for cells to commit to the EMT includes adjustments of the translation machinery and metabolic pathways to cope with a high demand of extracellular… More >

  • Open Access

    VIEWPOINT

    Possible mechanisms of bidirectional nuclear transport during neuronal migration

    CHUYING ZHOU1, MINEKO KENGAKU1,2,*
    BIOCELL, Vol.46, No.11, pp. 2357-2361, 2022, DOI:10.32604/biocell.2022.021050
    (This article belongs to this Special Issue: Tumor Microenvironment and Cytoskeletal Dynamics)
    Abstract Neuronal migration is a fundamental process of mammalian brain development. In migrating neurons, the nuclear membrane protein Nesprin-2 has been shown to serve as an adaptor to pull the nucleus along microtubule tracks. Current evidence has shown that Nesprin-2 binds to both the minus-end-directed motor dynein as well as the plus-end-directed motor kinesin. However, translocation of neuronal nucleus has long been thought to be primarily driven by dynein motors. Intriguing questions could be raised about the role of kinesin in nuclear transport and how the activities of opposing motors are coordinated through interactions with Nesprin. Combining evidence from recent studies,… More >

  • Open Access

    VIEWPOINT

    Mesenchymal stem cells and cell-free preparations for treating atopic dermatitis

    TRINIDAD MONTERO-VILCHEZ1,2,*, MANUEL SANCHEZ-DIAZ1,2, CAROLINA MONTERO-VILCHEZ3, ALVARO SIERRA-SANCHEZ2, SALVADOR ARIAS-SANTIAGO1,2,4
    BIOCELL, Vol.46, No.11, pp. 2363-2367, 2022, DOI:10.32604/biocell.2022.021399
    Abstract Atopic dermatitis (AD) is a chronic cutaneous inflammatory disease caused by an interaction between genetic, immune and epidermal barrier factors. Several treatments can be used to treat this disease but there are patients that do not respond to actual drugs. So, there is a need to develop effective therapies for AD. Mesenchymal stem cells (MSCs) are non-hematopoietic multipotent adult progenitor cells with immunomodulatory power and self-regenerating capacity to repair tissue damage, so they could be a potential effective treatment for AD. MSCs-Conditioned Medium (CM) and MSCs-exosomes are cell-free preparation with molecules secreted by stem cells that could be also beneficial… More >

  • Open Access

    ARTICLE

    Identification of SARS-CoV-2 by gold nanoparticles

    LORIS RIZZELLO1,2,3, VALERIA DE MATTEIS4,*
    BIOCELL, Vol.46, No.11, pp. 2369-2380, 2022, DOI:10.32604/biocell.2022.021059
    Abstract The SARS-CoV-2 outbreaks highlighted the need for effective, reliable, fast, easy-to-do and cheap diagnostics procedures. We pragmatically experienced that an early positive-case detection, inevitably coupled with a mass vaccination campaign, is a milestone to control the COVID-19 pandemic. Gold nanoparticles (AuNPs) can indeed play a crucial role in this context, as their physicochemical, optics and electronics properties are being extensively used in photothermal therapy (PTT), radiation therapy (RT), drug delivery and diagnostic. AuNPs can be synthesized by several approaches to obtain different sizes and shapes that can be easily functionalized with many kinds of molecules such as antibodies, proteins, probes,… More >

  • Open Access

    REVIEW

    Biomechanics of transendothelial migration by cancer cells

    CLAUDE VERDIER*
    BIOCELL, Vol.46, No.11, pp. 2381-2386, 2022, DOI:10.32604/biocell.2022.021368
    (This article belongs to this Special Issue: Tumor Microenvironment and Cytoskeletal Dynamics)
    Abstract Cancer metastasis is still a major social issue with limited knowledge of the formation of tumors and their growth. In addition the formation of metastases is very difficult to understand, since it involves very complex physical mechanisms such as cellular interactions and cell rheology, which are flow-dependent. Previous studies investigated transendothelial migration using sophisticated techniques such as microfluidics, traction force microscopy (TFM) or Atomic Force Microscopy (AFM), combined with physical modeling. Here we summarize recent results and suggest new ways to investigate the precise mechanisms used by cancer cells to undergo transendothelial migration. More >

  • Open Access

    REVIEW

    Effect of demethyltransferase FTO on tumor progression

    LING SHENG1,2, YUEHONG SHEN1,2, HONGYU YANG1,2,*
    BIOCELL, Vol.46, No.11, pp. 2387-2397, 2022, DOI:10.32604/biocell.2022.021032
    (This article belongs to this Special Issue: Recent Advancement in Cancer Molecular Signaling)
    Abstract N6-methyladenosine (m6A) modification is the most widespread and conserved internal mRNA modification in mammalian cells. It greatly affects genetic regulation by enhancing the involvement of diverse cellular enzymes and thus, plays a significant role in basic life processes. Numerous studies on m6A modification identified FTO as a crucial demethylase that participates in various biological processes. Not only does FTO play a pivotal role in obesity-related conditions, but it also influences the occurrence, development, and prognosis of several cancers, such as acute myeloid leukemia, breast cancer, liver cancer, and lung cancer. Moreover, FTO also shows a close association with immunity and… More >

  • Open Access

    ARTICLE

    Es-SOX8 regulates the morphological changes of the sperm nucleus of Eriocheir sinensis by activating Es-BMP2 transcription

    KE JIA1, FU-QING TAN2, SHUANG-LI HAO1,*, WAN-XI YANG1,*
    BIOCELL, Vol.46, No.11, pp. 2399-2413, 2022, DOI:10.32604/biocell.2022.022015
    Abstract The SRY-related high mobility group (HMG) box (SOX) transcription factors participate in many physiological processes of animal growth, development, and reproduction and are related to spermatogenesis in many species. However, the relationship between SOX and spermatogenesis in Eriocheir sinensis is rarely reported. Here, we studied the role of Es-SOX8 in the spermatogenesis of E. sinensis and its possible regulation mechanism. Immunofluorescence results demonstrated Es-SOX8 signal in both cytoplasm and nucleus of spermatogonia, spermatocytes as well as spermatids, but not in mature spermatozoa. Hematoxylin and eosin staining showed a significant increase in the number of spermatozoa with abnormal nuclear morphology   … More >

  • Open Access

    ARTICLE

    Exosomal miR-218 regulates the development of endometritis in dairy cows by targeting TGIF2/TGF-β pathway

    CHANG CHEN1,#, LIMIN QIAO2,#, KAIJUN GUO1, YINGQIU WANG1, MENGYI YUAN3, BOFAN FU4, XIAOBO GAO3, HEMIN NI1, LONGFEI XIAO1,*, XIANGGUO WANG1,*
    BIOCELL, Vol.46, No.11, pp. 2415-2423, 2022, DOI:10.32604/biocell.2022.021510
    (This article belongs to this Special Issue: Decoding Gene (including circRNA, lincRNA miRNA and mRNA) Expression)
    Abstract Endometritis affects the reproductive capacity of dairy cows and leads to serious economic losses in dairy farming. Clarification of the pathogenesis of endometritis is necessary to improve the reproductive efficiency of dairy cows. Exosomes and their miRNAs have been proven to play an important role in inflammatory regulation. Exosomal miR-218 is a differentially expressed miRNA found in endometrial epithelial cells (EECs) under endometrial inflammation. Therefore, we investigated the expression of miR-218 in the uterine tissue of dairy cows, lipopolysaccharide (LPS) treated EECs, exosomal vesicles, and regulation of exosomal miR-218 by targeting TGIF-2 inducible factor homology frame 2 (TGIF2)/transforming growth factor-beta… More >

  • Open Access

    ARTICLE

    3-epi-bufotalin suppresses the proliferation in colorectal cancer cells through the inhibition of the JAK1/STAT3 signaling pathway

    SANHUA LI1,2,#, QINGHONG KONG1,2,#, XIAOKE ZHANG1,2, XINTING ZHU1,3, CHUNBO YU3, CHANGYAN YU1,2, NIAN JIANG1,2, JING HUI1,2, LINGJIE MENG1,2,*, YUN LIU1,2,3,*
    BIOCELL, Vol.46, No.11, pp. 2425-2432, 2022, DOI:10.32604/biocell.2022.019916
    (This article belongs to this Special Issue: Recent Advancement in Cancer Molecular Signaling)
    Abstract Traditional Chinese medicine (TCM) has been increasingly employed in the last decades in China for both preventing and treating a variety of cancers. 3-epi-bufotalin is an active ingredient of TCM “Chanpi” with anti-tumor potential. However, the effect and mechanism of 3-epi-bufotalin on colorectal cancers were not well disclosed. The present study demonstrated that 3-epi-bufotalin could reduce viability, trigger apoptosis, and block the cell cycle at the G2/M stage in colorectal cancer cell lines HT29, RKO, and COLO205 in vitro. Moreover, 3-epi-bufotalin inhibited the JAK1/STAT3 signaling pathway. These results indicated the anti-proliferation ability of 3-epi-bufotalin in colorectal cancer cells. More >

  • Open Access

    ARTICLE

    Uridine dynamic administration affects the circadian variation of bile acid metabolism in high-fat-diet-fed mice

    TIANTIAN ZHOU1,3,#, YUMEI ZHANG1,2,#, JUAN ZHANG1,2, CHUNYAN XIE2, ZHENYA ZHAI1,2, XIN WU1,2,3,*
    BIOCELL, Vol.46, No.11, pp. 2433-2442, 2022, DOI:10.32604/biocell.2022.021290
    Abstract High-fat diet (HFD) is demonstrated to disturb the bile acid metabolism. The rhythm of bile acid metabolism can also be affected by uridine, whose metabolism exhibits a daily rhythm. However, the mechanism of dynamic uridine administration affecting bile acid during HFD remains unclear. In this study, C57BL/6J mice were fed HFD (the control group; CON) or HFD with oral administration of uridine in the daytime (DUR) and nighttime (NUR) to investigate the mechanism of the effect of uridine on the bile acid. This study showed that the mRNA expression of uridine transporters and circadian clock genes in the jejunum was… More >

  • Open Access

    ARTICLE

    Metformin alleviates LTA-induced inflammatory response through PPARγ/MAPK/NF-κB signaling pathway in bovine mammary epithelial cells

    ABDELAZIZ ADAM IDRISS ARBAB1,3,#, CHUNQING YIN4,#, XUBIN LU1, YAN LIANG1, ISMAIL MOHAMED ABDALLA1, AMER ADAM IDRIS3, TIANLE XU1,2, YONGJIANG MAO1, ZHANGPING YANG1,2,*
    BIOCELL, Vol.46, No.11, pp. 2443-2454, 2022, DOI:10.32604/biocell.2022.020865
    (This article belongs to this Special Issue: Decoding Gene (including circRNA, lincRNA miRNA and mRNA) Expression)
    Abstract Mastitis is a common inflammatory cow mammary infection; that causes significant economic loss in dairy industry. Given the interesting connection between metformin’s anti-inflammatory function and mastitis model induced by LTA in pbMECs, our objective was to prove that metformin was beneficial in suppressing proinflammatory response induced by LTA through modulation of mitogen-activated protein kinase (MAPK) and nuclear factor kappa B (NF-κB) signaling pathways and activation of peroxisome proliferator-activated receptor-γ (PPARγ) in pbMECs. The proliferation of cells and mRNA expression were measured using EdU assay and quantitative reverse transcriptase-polymerase chain reaction (qRT-PCR). Immunoblotting and immunofluorescence analysis were conducted to evaluate the… More >

  • Open Access

    ARTICLE

    Dexmedetomidine alleviates oxygen and glucose deprivation-induced apoptosis in mesenchymal stem cell via downregulation of MKP-1

    RUICONG GUAN1,3,#, KUAN ZENG1,#, MINNAN GAO1, JIANFEN LI1, HUIQI JIANG1, LU ZHANG1, JINGWEN LI1, BIN ZHANG1, YUQIANG LIU1, ZHUXUAN LIU1, DIAN WANG1, YANQI YANG1,2,*
    BIOCELL, Vol.46, No.11, pp. 2455-2463, 2022, DOI:10.32604/biocell.2022.021661
    Abstract Bone marrow mesenchymal stem cell (MSC)-based therapy is a novel candidate for heart repair. But ischemia-reperfusion injury leads to low viability of MSC. Dexmedetomidine (Dex) has been found to protect neurons against ischemia-reperfusion injury. It remains unknown if Dex could increase the viability of MSCs under ischemia. The present study is to observe the potential protective effect of Dex on MSCs under ischemia and its underlying mechanisms. Specific mRNAs related to myocardial ischemia in the GEO database were selected from the mRNA profiles assessed in a previous study using microarray. The most dysregulated mRNAs of the specific ones from the… More >

  • Open Access

    ARTICLE

    Isolation and species diversity of arbuscular mycorrhizal fungi in the rhizosphere of Puccinellia tenuiflora of Songnen saline-alkaline grassland, China

    YUNHUI ZHOU, YAJIE LIU, WENNA ZHAO, FEI CHEN, YUDAN WANG, CHUNXUE YANG
    BIOCELL, Vol.46, No.11, pp. 2465-2474, 2022, DOI:10.32604/biocell.2022.021016
    (This article belongs to this Special Issue: Mycorrhizal Fungal Roles in Stress Tolerance of Plants)
    Abstract Salinization has led to the deterioration of the ecological environment, affected the growth of plants, and hindered the development of agriculture and forestry. Arbuscular mycorrhizal (AM) fungi, as important soil microorganisms, play significant physiological and ecological roles in promoting plant nutrient absorption and improving soil structure. Puccinellia tenuiflora (Turcz.) Scribn. et Merr. in Songnen saline-alkaline grassland was selected as the research object to observe AM fungal colonization of the roots and explore the species and diversity of AM fungi in symbiotic association with P. tenuiflora. This study showed that AM fungi colonized in P. tenuiflora roots and formed a typical… More >

  • Open Access

    ARTICLE

    Significant changes in arbuscular mycorrhizal community and soil physicochemical properties during the saline-alkali grassland vegetation succession

    YAJIE LIU, LINLIN FANG, CHUNXUE YANG*
    BIOCELL, Vol.46, No.11, pp. 2475-2488, 2022, DOI:10.32604/biocell.2022.021477
    (This article belongs to this Special Issue: Mycorrhizal Fungal Roles in Stress Tolerance of Plants)
    Abstract Arbuscular mycorrhizal (AM) fungi are widely distributed in various habitats, and the community composition varies in response to the changing environmental conditions. To explore the response of community composition to the succession of saline-alkali land, soil samples were collected from three succession stages of Songnen saline-alkali grassland. Subsequently, the soil characteristics were determined and the AM fungi in soil samples were analyzed by high-throughput sequencing. Then, the response relationship between community composition and soil characteristics was studied by Canonical correlation and Pearson analyses. The soil properties improved with the succession of saline-alkali grassland. There was no significant difference in alpha… More >

  • Open Access

    ARTICLE

    Development of polymorphic SSR markers and their applicability in genetic diversity evaluation in Euptelea pleiosperma

    XIAOJUN ZHOU*, XIAOYU LU, XUBO WANG
    BIOCELL, Vol.46, No.11, pp. 2489-2495, 2022, DOI:10.32604/biocell.2022.020311
    Abstract Euptelea pleiosperma is a characteristic species of East Asian flora with both ornamental and scientific values. Based on the reduced-representation sequencing (RRS) technology of RAD-Seq, this study conducted high-throughput Illumina paired-end sequencing to find SSR marker information in the genome of E. pleiosperma, and to screen and verify polymorphism of SSR markers. We obtained 5.5G of high-quality data using RAD-Seq. The total number of contigs of the RAD tags was 299,376, with the maximum contig length of 2,062 bp and the average length of 445 bp. From these sequences, we identified 20,718 SSR loci, with a distribution density of one… More >

  • Open Access

    ARTICLE

    Application of two forms of silicon and their impact on the postharvest and the content of bioactive compounds in cucumber (Cucumis sativus L.) fruits

    YOLANDA GONZÁLEZ-GARCÍA1, VALERIA FLORES-ROBLES2, GREGORIO CADENAS-PLIEGO3, ADALBERTO BENAVIDES-MENDOZA4, MARCELINO CABRERA DE LA FUENTE4, ALBERTO SANDOVAL-RANGEL4, ANTONIO JUÁREZ-MALDONADO1,*
    BIOCELL, Vol.46, No.11, pp. 2497-2506, 2022, DOI:10.32604/biocell.2022.021861
    (This article belongs to this Special Issue: Physiology and Molecular Biology of Plant Stress Tolerance)
    Abstract The metabolic activity of the fruits continues even after harvest, which results in the loss of bioactive compounds, a decrease in the quality of the fruits, softening and browning, among other negative effects. The use of certain elements such as silicon can improve postharvest quality, since it is involved in the metabolic, physiological and structural activity of plants, moreover can increase the quality of the fruits. In addition, nanotechnology has had a positive impact on crop yield, nutritional value, fruit quality and can improve antioxidant activity. For these reasons, the use of beneficial elements such as silicon in the form… More >

  • Open Access

    ARTICLE

    In vivo protective effect of late embryogenesis abundant protein (ApSK3 dehydrin) on Agapanthus praecox to promote post-cryopreservation survival

    TINGTING HUANG1,#, SHAN DENG2,#, JIANGYUAN SHENG1, DI ZHANG1,*
    BIOCELL, Vol.46, No.11, pp. 2507-2515, 2022, DOI:10.32604/biocell.2022.021314
    (This article belongs to this Special Issue: Physiology and Molecular Biology of Plant Stress Tolerance)
    Abstract Dehydrins (DHNs), as members of the late embryogenesis abundant protein family, play critical roles in the protection of seeds from dehydration and plant adaptation to multiple abiotic stresses. Vitrification is a basic method in plant cryopreservation and is characterized by forming a glassy state to prevent lethal ice crystals produced during cryogenic storage. In this study, ApSK3 type DHN was genetically transformed into embryogenic calluses (EC) of Agapanthus praecox by overexpression (OE) and RNA interference (RNAi) techniques to evaluate the in vivo protective effect of DHNs during cryopreservation. The cell viability showed a completely opposite trend in OE and RNAi… More >

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