Home / Journals / BIOCELL / Vol.31, No.1, 2007
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  • Open Access

    ARTICLE

    Review : Biochemical-molecular markers in unilateral ureteral obstruction

    WALTER MANUCHA
    BIOCELL, Vol.31, No.1, pp. 1-12, 2007, DOI:10.32604/biocell.2007.31.001
    Abstract Congenital obstructive nephropathy is the primary cause of end-stage renal disease in children. Rapid diagnosis and initiation of the treatment are vital to preserve function and/or to slow down renal injury. Obstructive uropathy effects -decline in the plasmatic renal flow and glomerular filtration rate, interstitial infiltrate of leukocytes, significant decrease of the urine concentration, loss of the capacity to concentrate urine as well as fibrosis and apoptosis- are a consequence of a variety of factors that work in complex ways and are still not fully understood. Mediators as angiotensin II, transforming growth factor-β (TGF-β) and nitric oxide (NO) have been… More >

  • Open Access

    ARTICLE

    Pollen morphology of Oxalis species from Buenos Aires province (Argentina)

    SONIA ROSENFELDT, BEATRIZ G. GALATI
    BIOCELL, Vol.31, No.1, pp. 13-21, 2007, DOI:10.32604/biocell.2007.31.013
    Abstract Pollen morphology and the degree of pollen variability within nine species and two varieties of Oxalis species from Buenos Aires Province (Argentina) were studied using light microscopy (LM), scanning electron microscopy (SEM) and transmission electron microscopy (TEM).
    Oxalis pollen grain is generally 3-colpate and the shape is prolate spheroidal, oblate spheroidal or spheroidal. The exine is microreticulate. The brochi are circular to polygonal; brochi sizes decrease near the colpi. The colpus membrane observed in unacetolysed material, is crustate or granular. The colpus length varies within a species; the largest grains are produced in stamens with long filaments and the smallest… More >

  • Open Access

    ARTICLE

    Localization of Sweet potato chlorotic stunt virus (SPCSV) in synergic infection with Potyviruses in sweet potato

    CLAUDIA FERNANDA NOME1 , SERGIO FERNANDO NOME2 , FABIANA GUZMÁN1 , LUIS CONCI1, IRMA GRACIELA LAGUNA1,2
    BIOCELL, Vol.31, No.1, pp. 23-31, 2007, DOI:10.32604/biocell.2007.31.023
    Abstract Among diseases reported worldwidely for sweet potato (Ipomoea batatas (L) Lam) crop, one of the most frequent is the Sweet potato virus disease (SPVD), caused by sweet potato chlorotic stunt virus (SPCSV) and sweet potato feathery mottle virus (SPFMV) co-infection. In Argentina, there exists the sweet potato chlorotic dwarf (SPCD), a sweet potato disease caused by triple co-infection with SPCSV, SPFMV and sweet potato mild speckling virus(SPMSV). Both diseases cause a synergism between the potyviruses(SPFMV and SPMSV) and the crinivirus (SPCSV). Up to date, studies carried out on the interaction among these three viruses have not described their localization in… More >

  • Open Access

    ARTICLE

    Effect of age on the myosin-V immunoreactive myenteric neurons of rats ileum

    JOÃO PAULO FERREIRA SCHOFFEN, MARIA RAQUEL MARÇAL NATALI
    BIOCELL, Vol.31, No.1, pp. 33-39, 2007, DOI:10.32604/biocell.2007.31.033
    Abstract Alterations in the gastrointestinal neuromuscular function related to age have been demonstrated in human and animal models. This study analyzes the effects of the aging process on the area of the neuronal cell bodies of the myenteric plexus in the antimesenteric and intermediate regions of the ileal circumference of Wistar, 12 month-old in comparison 3 month-old animals. The ileum was removed and wholemount preparations immunostained by the antibody anti-myosin-V were processed. The morphometric analyses were performed using a computerized image analysis system, with a subsequent distribution of neurons by size in intervals of 100 μm2. The cellular body morphometry revealed… More >

  • Open Access

    ARTICLE

    Behavior and preservation of an in vitro collection of European aspen in Spain

    M.T. MARTIN*, H.E. PEDRANZANI**, R. SIERRA DE GRADO
    BIOCELL, Vol.31, No.1, pp. 41-49, 2007, DOI:10.32604/biocell.2007.31.041
    Abstract An in vitro collection has been established with selected European aspen from Palencia province (Spain). Currently, this collection includes 32 high quality clones, selected for their good bearing and healthy state. Most of them belong to different discrete local populations.
    Populus tremula L. was propagated in proliferation Aspen Culture Medium; they required subculture every 3 months. The purpose of this study was, therefore, to select a medium which allows the maintenance of 32 clones for a period longer than 3 months without subculture and to observe the behavior of those clones in 15 different culture medium compositions. Seven nodal cutting… More >

  • Open Access

    ARTICLE

    Preimplantation embryotoxicity after mouse embryo exposition to reactive oxygen species

    ELISA CEBRAL1,2, ISABEL CARRASCO1 , DAVID VANTMAN1, ROSITA SMITH1
    BIOCELL, Vol.31, No.1, pp. 51-59, 2007, DOI:10.32604/biocell.2007.31.051
    Abstract Exposure of either gametes or embryos to conditions and/or factors that generate oxidative stress has been associated with impaired early embryogenesis. The effects of reactive oxygen species (ROS) on mouse preimplantation development, depending of the ROS-concentration and time of exposition, were studied. Two-cell embryos were incubated with 5, 10, 25 and 50 μM of hydrogen peroxide (H2O2) for 30 and 60 minutes of exposition and allowed to develop for 72 h to study the quality of development. The incubation with 50 μM H2O2 for 30 or 60 minutes, strongly inhibited the 2-cell embryo development as compared to the control (p<0.001).… More >

  • Open Access

    ARTICLE

    Endogenous ADP-ribosylation of eukaryotic elongation factor 2 and its 32 kDa tryptic fragment

    KIVANÇ ERGEN*, MUHAMMET BEKTAŞ**, SINA GÖKÇE**, RÜSTEM NURTEN**
    BIOCELL, Vol.31, No.1, pp. 61-66, 2007, DOI:10.32604/biocell.2007.31.061
    Abstract Eukaryotic elongation factor 2 (eEF-2) can undergo ADP-ribosylation in the absence of diphtheria toxin. The binding of free ADP-ribose and endogenous transferase-dependent ADP-ribosylation were distinct reactions for eEF-2, as indicated by different findings. Incubation of eEF-2 tryptic fragment 32/33 kDa (32F) with NAD was ADP-ribosylated and gave rise to the covalent binding of ADP-ribose to eEF-2. 32F was revealed to be at the C-terminal by Edman degradation sequence analysis.
    In our study, the elution of 32F from SDS-PAGE was ADP-ribosylated both in the presence and absence of diphtheria toxin. These results suggest that endogenous ADP-ribosylation of 32F might be related… More >

  • Open Access

    ARTICLE

    Effects of prenatal stress on male offspring sexual maturity

    NANCY RODRÍGUEZ, NORA MAYER, HÉCTOR F. GAUNA
    BIOCELL, Vol.31, No.1, pp. 67-74, 2007, DOI:10.32604/biocell.2007.31.067
    Abstract Prenatal stimulations have been shown to have long-term effects on at reproductive activity. We evaluated the influence of the prenatal stress on the hypothalamic-pituitary-gonad (HPG) axis in male offsprings from mothers with high number of offsprings per litter (HNL) and low number of offsprings per litter (LNL) after hypothesizing that the number of offsprings per litter may modify the effect of the prenatal stress on the HPG of adult offsprings. Pregnant Wistar rats were used for this study. Immobilization (IMO) stress was used, 30 min, 3 times per week, from the 5th to 21st day of pregnancy. The weight of… More >

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