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Localization of Sweet potato chlorotic stunt virus (SPCSV) in synergic infection with Potyviruses in sweet potato

CLAUDIA FERNANDA NOME1 , SERGIO FERNANDO NOME2 , FABIANA GUZMÁN1 , LUIS CONCI1, IRMA GRACIELA LAGUNA1,2
1. Instituto de Fitopatología y Fisiología Vegetal. IFFIVE-Instituto Nacional de Tecnología Agropecuaria (INTA). Córdoba. Argentina.
2. CONICET.
Address correspondence to: Dra. Claudia F. Nome. Instituto de Fitovirología y Fisiología Vegetal (IFFIVE-INTA). Camino 60 Cuadras Km 5 1/2. (X5020ICA) Córdoba, ARGENTINA. E-mail: cnome@correo.inta.gov.ar

BIOCELL 2007, 31(1), 23-31. https://doi.org/10.32604/biocell.2007.31.023

Abstract

Among diseases reported worldwidely for sweet potato (Ipomoea batatas (L) Lam) crop, one of the most frequent is the Sweet potato virus disease (SPVD), caused by sweet potato chlorotic stunt virus (SPCSV) and sweet potato feathery mottle virus (SPFMV) co-infection. In Argentina, there exists the sweet potato chlorotic dwarf (SPCD), a sweet potato disease caused by triple co-infection with SPCSV, SPFMV and sweet potato mild speckling virus(SPMSV). Both diseases cause a synergism between the potyviruses(SPFMV and SPMSV) and the crinivirus (SPCSV). Up to date, studies carried out on the interaction among these three viruses have not described their localization in the infected tissues. In single infections, virions of the crinivirus genus are limited to the phloem while potyviral virions are found in most tissues of the infected plant.
The purpose of this work was to localize the heat shock protein 70 homolog (HSP70h), a movement protein for genus crinivirus, of an Argentinean SPCSV isolate in its single infection and in its double and triple co-infection with SPFMV and SPMSV. The localization was made by in situ hybridization (ISH) for electron microscopy (EM) on ultrathin sections of sweet potato cv. Morada INTA infected tissues.
The results demonstrated that viral RNA coding HSP70h is restricted to phloem cells during crinivirus single infection, while it was detected outside the phloem in infections combined with the potyviruses involved in chlorotic dwarf disease.

Keywords

Sweet potato chlorotic stunt virus, HSP70h, in situ hybridization, crinivirus, sweet potato

Cite This Article

NOME, C. F., LAGUNA, I. G. (2007). Localization of Sweet potato chlorotic stunt virus (SPCSV) in synergic infection with Potyviruses in sweet potato. BIOCELL, 31(1), 23–31.

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This work is licensed under a Creative Commons Attribution 4.0 International License , which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
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