Home / Journals / BIOCELL / Vol.36, No.3, 2012
Table of Content
  • Open Access

    ARTICLE

    Effects of rotational culture on morphology, nitric oxide production and cell cycle of endothelial cells

    CHAOJUN TANG1, XUE WU1, LINQI YE1,2, XIANG XIE1, GUIXUE WANG1*
    BIOCELL, Vol.36, No.3, pp. 97-104, 2012, DOI:10.32604/biocell.2012.36.097
    Abstract Devices for the rotational culture of cells and the study of biological reactions have been widely applied in tissue engineering. However, there are few reports exploring the effects of rotational culture on cell morphology, nitric oxide (NO) production, and cell cycle of the endothelial cells from human umbilical vein on the stent surface. This study focuses on these parameters after the cells are seeded on the stents. Results showed that covering of stents by endothelial cells was improved by rotational culture. NO production decreased within 24 h in both rotational and static culture groups. In addition, rotational culture significantly increased… More >

  • Open Access

    ARTICLE

    Reproductive performance of the Mesa silverside (Chirostoma jordani Woolman, 1894) under natural and controlled photoperiods

    JOSÉ LUIS ARREDONDO-FIGUEROA1, LAURA GEORGINA NÚÑEZ-GARCÍA2, PALOMA ADRIANA HEREDIA-GUZMÁN3 AND JESÚS T. PONCE-PALAFOX4.
    BIOCELL, Vol.36, No.3, pp. 105-111, 2012, DOI:10.32604/biocell.2012.36.105
    Abstract Chirostoma jordani is a native annual species inhabiting lacustrine waters of the Central Mexico Plateau. It is widely distributed and is currently facing high environmental pressures. Five experiments were performed to study the reproductive performance of this species. Four of the experiments were conducted in 270-L indoor recirculation tanks. Two males and one female at the first stage of reproduction were included in each test. A photoperiod of 14 light hours and 10 dark hours was used. In a fifth experiment, 10 females and 15 males were kept in an outdoor 3,000-L recirculation tank under natural photoperiod. The number of… More >

  • Open Access

    ARTICLE

    Mechanisms involved in the cytotoxic effects of berberine on human colon cancer HCT-8 cells

    LI-NA XU1#, BI-NAN LU2#, MING-MING HU1, YOU-WEI XU1, XU HAN1, YAN QI1, JIN-YONG PENG1,3*
    BIOCELL, Vol.36, No.3, pp. 113-120, 2012, DOI:10.32604/biocell.2012.36.113
    Abstract Berberine, a constituent of some traditional Chinese medicinal plants, has been reported to have cytotoxicity effects on different human cancer cell lines. There is no available information about the effects and mechanism of action of berberine on human colon cancer cell line HCT-8. In this paper, the cytotoxicity of berberine on HCT-8 cancer cells was investigated by MTT assay, fluorescence microscopy and flow cytometry analysis. Our data revealed that berberine could significantly inhibit the growth of HCT-8 cells in a dose- and time-dependent manner. Morphology of apoptotic cells was studied with acridine orange/ ethidium bromide staining. The concentrations of lactate… More >

  • Open Access

    ARTICLE

    Knockdown of apoptosis-inducing factor disrupts function of respiratory complex

    MIROSLAV VAŘECHA1*, DANIELA PÁCLOVÁ2, JIŘINA PROCHÁZKOVÁ2, PAVEL MATULA1, DUŠAN CMARKO3, AND MICHAL KOZUBEK1
    BIOCELL, Vol.36, No.3, pp. 121-126, 2012, DOI:10.32604/biocell.2012.36.121
    Abstract Recent findings suggest that apoptotic protein apoptosis-inducing factor (AIF) may also play an important non-apoptotic function inside mitochondria. AIF was proposed to be an important component of respiratory chain complex I that is the major producer of superoxide radical. The possible role of AIF is still controversial. Superoxide production could be used as a valuable measure of complex I function, because the majority of superoxide is produced there. Therefore, we employed superoxide-specific mitochondrial fluorescence dye for detection of superoxide production. We studied an impact of AIF knockdown on function of mitochondrial complex I by analyzing superoxide production in selected cell… More >

  • Open Access

    ARTICLE

    RETRACTED: A likely role for a novel PH-domain containing protein, PEPP2, in connecting membrane and cytoskeleton

    YI ZOU AND WENPING ZHONG
    BIOCELL, Vol.36, No.3, pp. 127-132, 2012, DOI:10.32604/biocell.2012.36.127
    Abstract The published article titled “A likely role for a novel PH-domain containing protein, PEPP2, in connecting membrane and cytoskeleton” has been retracted from the BIOCELL, Vol. 36, Issue 3, 2012. Title: A likely role for a novel PH-domain containing protein, PEPP2, in connecting membrane and cytoskeleton Authors: Yi Zou and Wenping Zhong URL: http://150.109.118.215/uploads/attached/file/20190102/20190102065508_87612.pdf The article “A likely role for a novel PH-domain containing protein, PEPP2, in connecting membrane and cytoskeleton” (Biocell 36, 127-132, 2012) has been retracted after publication by decision of the Editor-in-Chief, after he received compelling evidence indicating that the article’s content was part of a doctoral… More >

  • Open Access

    ARTICLE

    Structural analysis of flagellar axonemes from inner arm dynein knockdown strains of Trypanosoma brucei

    RANDI ZUKAS1, ALEX J. CHANG1, MARIAN RICE2, AMY L. SPRINGER1*
    BIOCELL, Vol.36, No.3, pp. 133-142, 2012, DOI:10.32604/biocell.2012.36.133
    Abstract Trypanosoma brucei is a protozoan flagellate that causes African sleeping sickness. Flagellar function in this organism is critical for life cycle progression and pathogenesis, however the regulation of flagellar motility is not well understood. The flagellar axoneme produces a complex beat through the precisely coordinated firing of many proteins, including multiple dynein motors. These motors are found in the inner arm and outer arm complexes. We are studying one of the inner arm dynein motors in the T. brucei flagellum: dynein-f. RNAi knockdown of genes for two components of dynein-f: DNAH10, the α heavy chain, and IC138, an intermediate chain,… More >

  • Open Access

    ARTICLE

    Pollen viability of Polygala paniculata L. (Polygalaceae) using different staining methods

    VIVIANE DAL-SOUTO FRESCURA1, HAYWOOD DAIL LAUGHINGHOUSE IV2, THAIS SCOTTI DO CANTODOROW1, SOLANGE BOSIO TEDESCO1*
    BIOCELL, Vol.36, No.3, pp. 143-145, 2012, DOI:10.32604/biocell.2012.36.143
    Abstract Polygala paniculata L. is a medicinal plant that grows in the Brazilian Atlantic coast, known as ‘barba-de-São-João’, ‘barba-de-bode’, ‘vassourinha branca’, and ‘mimosa’. In this study, pollen viability was estimated by three different staining methods: 2% acetic orcein, 2% acetic carmine, and Alexander’s stain. The young inflorescences of twenty accessions were collected and fixed in a solution of ethanol: acetic acid (3:1) for 24 hours, then stored in ethanol 70% under refrigeration. Six slides per plant, two for each stain, were prepared by squashing, and 300 pollen grains per slide were analyzed. Pollen viability was high (>70%) for most accessions of… More >

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