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REVIEW

EMILIA MANOLE^1,2,#, LAURA DUMITRESCU^2,3,#, CRISTINA NICULIȚE^1,3, BOGDAN OVIDIU POPESCU^1,2,3, LAURA CRISTINA CEAFALAN^1,3,*
BIOCELL, Vol.45, No.1, pp. 1-16, 2021, DOI:10.32604/biocell.2021.013452
Abstract An increasing number of studies provide evidence for the existence of a microbiota-gut-brain axis and its potential involvement in the development of sporadic Parkinson’s disease and other neurodegenerative conditions. The neuropathologic hallmark of Parkinson’s disease is the presence of brain intraneuronal aggregates of misfolded alpha-synuclein, known as Lewy bodies. Some gut microbiota products may trigger alpha-synuclein conformational changes in the neurons of the enteric nervous system, which can then spread to the brain in a prion-like fashion through the vagus nerve. Others may interfere with neuroinflammatory pathways and susceptibility to neurodegeneration. In this review, we assess the potential role of… More >

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MERVIN BURNETT¹, VITO RODOLICO², FAN SHEN¹, ROGER LENG¹, MINGYONG ZHANG³, DAVID D. EISENSTAT^4,5, CONSOLATO SERGI^1,3,6,*
BIOCELL, Vol.45, No.1, pp. 17-26, 2021, DOI:10.32604/biocell.2021.013973
（This article belongs to this Special Issue: Molecular and Cellular Diagnostic Models)
Abstract MicroRNAs (miRNAs) are small single-stranded, non-coding RNA molecules involved in the pathogenesis and progression of cancer, including osteosarcoma. We aimed to clarify the pathways involving miRNAs using new bioinformatics tools. We applied WikiPathways and PathVisio, two open-source platforms, to analyze miRNAs in osteosarcoma using miRTar and ONCO.IO as integration tools. We found 1298 records of osteosarcoma papers associated with the word “miRNA”. In osteosarcoma patients with good response to chemotherapy, miR-92a, miR- 99b, miR-193a-5p, and miR-422a expression is increased, while miR-132 is decreased. All identified miRNAs seem to be centered on the TP53 network. This is the first application of… More >

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ARTICLE

PENG LIU^1,2, ANFANG ZOU³, QI CHEN⁴, BIAO CHENG^1,*, QIN LI^1,*
BIOCELL, Vol.45, No.1, pp. 27-39, 2021, DOI:10.32604/biocell.2021.012601
Abstract The diabetic ulcer is one of the serious complications of diabetes. In this study, we aimed to establish an exosomal microRNA (miRNA)-targeted messenger RNA (mRNA) regulatory network for screening new biomarkers for diabetic ulcer treatment. For this purpose, exosomes were extracted from bone marrow stem cells (BMSCs) collected from diabetic ulcer patients and healthy adults. The miRNAs in exosomes was detected by high-throughput sequencing analysis. The Gene Ontology (GO) and Kyoto Encyclopedia of Genes and Genomes (KEGG) pathway enrichment analysis of the differential miRNAs were performed. The miRNA-mRNA regulatory network between candidate miRNAs and their target genes were constructed by… More >

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YONGLI ZHANG^1,#, YIZHAN CAO^2,#, BO ZHU³, YANNI JIANG³, PENGCHONG LIANG^3,*
BIOCELL, Vol.45, No.1, pp. 41-48, 2021, DOI:10.32604/biocell.2021.010454
Abstract Glycosylation of mucins mediated by N-acetylgalactosaminyltransferases (GALNTs) is closely related to respiratory diseases such as asthma and chronic obstructive pulmonary disease (COPD). In addition, long non-coding RNAs (LncRNAs) participate in physiological and pathological processes through various epigenetic mechanisms. In this study, we found that a novel LncRNA named NKILA combined with multiple mucins and GALNTs potentially by several bioinformatics methods, and we used quantitative real-time PCR (RT-qPCR) to detect the expressions of NKILA, MUC5AC, MUC5B, and GALNT2 mRNA in 50 cases of asthma samples and 19 cases of normal samples, whose results showed that the expression of NKILA was significantly… More >

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1001

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FEI TIAN^*, GANG LIU, LINLIN FAN, ZHONGYUN CHEN, YAN LIANG,
BIOCELL, Vol.45, No.1, pp. 49-56, 2021, DOI:10.32604/biocell.2021.013794
Abstract Cerebral ischemia is due to the formation of blood clots or embolisms in the brain arteries, which leads to local brain tissue necrosis and neural cell apoptosis. Recent studies have shown that microRNA (miRNA) plays an important regulatory role in the pathological process of ischemic injury. The aim of this study is to investigate the role and the mechanism of miR-21-3p and drosophila mothers against decapentaplegic 2 (SMAD2) in cerebral ischemic reperfusion injured (CIRI) neural cells. The CIRI model was established by oxygen-glucose deprivation and recovery process for N2a cells. The cell viability and the apoptotic was evaluated by MTT… More >

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ARTICLE

YANGUO PENG^1,#, WEI LI^2,#, SHUIYU CHEN¹, WANGCHUN WU¹, ZHANFANG SHANG^1,*
BIOCELL, Vol.45, No.1, pp. 57-64, 2021, DOI:10.32604/biocell.2021.011756
Abstract Peripheral nerve injury is a common neurodegenerative disease, which causes disability and a huge economic burden for patients. MicroRNAs (miRNAs) have been acknowledged as major regulators and therapeutic targets of neurological disease. Thus, the functional studies of miRNAs in neurological disease will contribute to discover new therapeutic targets for peripheral nerve injury. Sprague Dawley rats treated sciatic nerve surgical injury were regarded as peripheral nerve injury model in vivo. The expression of miR-494-3p and Kruppel like factor7 (KLF7) were measured by Real-time quantitative polymerase chain reaction (RT-qPCR) assay. In addition, western blot analysis was conducted to measure the protein levels… More >

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ARTICLE

JIA-FONG HONG¹, BAGHDAD OUDDANE², JIANG-SHIOU HWANG^3,4,5, HANS-UWE DAHMS^1,6,7,*
BIOCELL, Vol.45, No.1, pp. 65-77, 2021, DOI:10.32604/biocell.2021.014154
（This article belongs to this Special Issue: Biomonitoring of Toxic Effects Using OMICs Markers)
Abstract Harmful algal blooms (HABs) that are formed by cyanobacteria have become a serious issue worldwide in recent years. Cyanobacteria can release a type of secondary metabolites called cyanotoxins into aquatic systems which may indirectly or directly provide health risks to the environment and humans. Cyanotoxins provide some of the most powerful natural poisons including potent neurotoxins, hepatotoxins, cytotoxins, and endotoxins that may result in environmental health risks, and long-term morbidity and mortality to animals and humans. In this research, we used the chemcomputational tool Molinspiration for molecular property predictions, Pred-hERG 4.2 web software for cardiac toxicity prediction, and Pred-Skin 2.0… More >

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ARTICLE

YONG LIU^1,2,#, ZHIHUA JU^1,#, QIANG JIANG¹, WENHAO LIU¹, CHUNHONG YANG¹, YARAN ZHANG¹, XIUGE WANG¹, YAPING GAO¹, XIAOCHAO WEI¹, YAN SUN¹, JINPENG WANG¹, MINGHAI HOU¹, LING YANG², JINMING HUANG^1,*
BIOCELL, Vol.45, No.1, pp. 79-87, 2021, DOI:10.32604/biocell.2021.013583
Abstract F-box and WD-40 domain protein 11 (FBXW11) is an important component of the E3 ubiquitin-ligase enzyme that plays a key role in the ubiquitin-dependent regulation of spermatogenesis. In our previous research, the mRNA expression of FBXW11 in bull sperm with high motility is significantly higher than that with low motility. In the present study, the protein expression levels of FBXW11 in bull testicular tissues with low-performance sperm quality groups were significantly higher than those in normal performance groups. The immunohistochemistry result demonstrated that FBXW11 protein was located in the periphery of Leydig cells and seminiferous tubules. Three splice variants of… More >

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ARTICLE

ATTALLA EL-KOTT^1,2, EMAN ELBEALY³, FAHMY ELSAID^1,4, HAITHAM EL-MEKKAWY¹, ABD-EL-KARIM ABD-LATEIF⁵, ABDULALI TAWEEL⁶, HEBA KHALIFA², AHMAD KANDEEL⁵, KAREEM MORSY^1,7, ESSAM IBRAHIM^1,8,9, MASHAEL MOHAMMED BIN-MEFERIJ^10,*
BIOCELL, Vol.45, No.1, pp. 89-101, 2021, DOI:10.32604/biocell.2021.012516
Abstract This study investigates if the anti-tumor effect of Pterostilbene in the SKOV3 ovarian cancer (OC) cell line involves inhibition of cell metabolism and tested in this effect involves modulating AMPK and Akt-induced regulation of mTORC1. Initially, SKOV3 cells were cultured in the humidified conditions in DMEM media for 24 h with or without increasing concentration of Pterostilbene. Then, the cells were incubated with Pterostilbene (IC₅₀ = 50 µM) under similar conditions with or without pre-incubation with Dorsomorphin, an AMPK inhibitor. In a dose-dependent manner, Pterostilbene inhibited SKOV3 cell survival and increased their lysate levels of lactate dehydrogenase (LDH) and single-stranded… More >

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ARTICLE

PING YANG, LIJUAN YUAN, SHUJIA PENG, YANMING DONG, LIN YANG, XI’E HU, GUOQIANG BAO^*
BIOCELL, Vol.45, No.1, pp. 103-108, 2021, DOI:10.32604/biocell.2021.011220
Abstract This study aimed to evaluate the association between the CLEC3A gene polymorphisms (rs2735401/rs2293776/ rs2072665) and the gastric cancer risk in the Northwestern Chinese population. A hospital-based case-control study was conducted on 681 cases and 756 healthy controls. Odds ratio (OR) and 95% confidence intervals (CI) were applied to evaluate the association of the CLEC3A polymorphisms on gastric cancer risk. We found that there was no significant association between the CLEC3A polymorphisms and gastric cancer susceptibility, which was detected in the main analysis or stratification analyses of age, gender, and clinical stages. Our findings verified that the CLEC3A polymorphisms are not… More >

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ARTICLE

PENG WU^#, MANLI GAO^#, JIANJIAN DONG, CHENCHEN XU, BO LI, XUN WANG, YONGZHU HAN, NAN CHENG^*
BIOCELL, Vol.45, No.1, pp. 109-117, 2021, DOI:10.32604/biocell.2021.012048
Abstract Wilson disease (WD), known as hepatolenticular degeneration (HLD), is a treatable autosomal recessive disorder of copper metabolism. Because copper deposits in the liver first, the liver is not only the original defective organ but also the most affected organ. The liver injury is also one of the main causes of death throughout the course of the disease. Therefore, the treatment of liver injury is the main task of WD treatment, which is of great significance to improve the prognosis of patients. Autophagy is a process that promotes cell survival through degradation, recycling, and absorption in order to maintain the normal… More >

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SALLY NEGM^1,2, MOHAMMAD EL-METWALLY³, WESAM ELDIN SABER⁴, SAHAR ABO-NEIMA⁵, MAHMOUD MOUSTAFA^6,7,*, ATTALLA EL-KOTT^6,8
BIOCELL, Vol.45, No.1, pp. 119-127, 2021, DOI:10.32604/biocell.2021.012011
（This article belongs to this Special Issue: New Insights in Biology of Depression: New Molecules and Approaches)
Abstract The need for the bacterial proteinase is rapidly growing, urging to catch a lowcost medium for the microbial fermentation, nanoparticles can play a vital role in this respect. The proteinase of Talaromyces purpureogenus was produced on the tubers of Helianthus tuberosus that also operated as solid support for the fermentation process. The interface amongst nitrogen sources (NH₄Cl and yeast extract) was investigated, applying the statistical modeling of central composite design under solid-state fermentation. The optimum medium for proteinase secretion was stimulated by 979.82 mg NH₄Cl and 437.68 mg yeast extract per 100 g substrate, yielding 108.15 U/g tubers. Using Plackett-Burman… More >

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ARTICLE

YUN SUN^#, YI LIU^#, BINHUA XUE, XIAODIE WANG, WEILI YU^*
BIOCELL, Vol.45, No.1, pp. 129-138, 2021, DOI:10.32604/biocell.2021.013275
Abstract Hyperlipidemia acute pancreatitis (HLAP) is a significant cause of AP, characterized by recurrent attacks, more complications and high incidence and mortality. HLAP is often accompanied by single or multiple organ damage. Negative regulation of interferon-regulatory factor 9 (IRF9) on sirtuin-1 (SIRT1) contributes to a range of diseases. However, the function of IRF9 and SIRT1, and the relationship of the two in HLAP with heart injury remain to be illustrated so far. Animal models of HLAP were set up by feeding with high-fat chow and subsequently injecting 20% L-arginine intraperitoneally. The degree of pancreas and heart tissue injury was evaluated. Heart… More >

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ARTICLE

ABDUR RAUF^1,*, SAUD BAWAZEER^2,*, MUSLIM RAZA³, EMAN EL-SHARKAWY⁴, MD. HABIBUR RAHMAN^5,6, MOHAMED A. EL-ESAWI⁷, GHIAS UDDIN⁸, BINA S. SIDDIQUI⁹, ANEES AHMED KHALIL¹⁰, JOSEPH MOLNAR¹¹, AKOS CSONKA¹¹, DIÁNA SZABÓ¹², HAROON KHAN¹³, MOHAMMAD S. MUBARAK¹⁴, TAIBI BEN HADDA¹⁵, MUDYAWATI KAMARUDDIN¹⁶, SEEMA PATEL¹⁷
BIOCELL, Vol.45, No.1, pp. 139-147, 2021, DOI:10.32604/biocell.2021.013277
Abstract The bioactive triterpenoid 3-oxo-6-β-hydroxy-β-amyrin (1) has been isolated from multiple plant sources. In this study, chloroform fraction of Pistacia integerrima extract was processed for the isolation of the compound. The compound identity was confirmed by advanced spectroscopy technique. X-ray crystallography was applied for molecular structure confirmation. In addition, compound 1 was screen for its activity on reversal of MDR (multidrug resistance) mediated by P-gp (P-glycoprotein). This was accomplished by using rhodamine123 exclusion on multidrug-resistant human ABCB1 gene transfected mouse T-lymphoma cell line. Outcomes revealed that MDR reversing effect was comparable to verapamil as positive control in vitro. Treatment of TPA-induced… More >

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ARTICLE

DONGYUE ZHANG, LINA WANG, HAO WANG, XIAOQIAN LV, QIAN REN, GUOGUANG ZHENG^*
BIOCELL, Vol.45, No.1, pp. 149-156, 2021, DOI:10.32604/biocell.2021.010334
Abstract Legumain is a C13 family cysteine protease. It plays diverse roles under both physiological and pathological conditions. The high-level expression of legumain is detected in solid tumors. Legumain promotes the proliferation and migration of tumor cells. However, the effect of legumain in blood diseases has not been established. In this report, we studied the effect of legumain on leukemia cells by overexpressing it in THP1 cells. The results demonstrated that legumain promoted cell proliferation, whereas it had little effect on cell apoptosis. Furthermore, legumain promoted the migration of THP1 cells. It was worth noting that legumain decreased the stemness of… More >

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ARTICLE

ZHIXIONG WANG^1,2, NA RISU², JIAYU FU², HUI LIU³, GUOMIN ZHOU³, QIAN LIU³, YAN ZOU⁴, JIAXING TANG⁴, LONG LI⁴, XUEKAI ZHU^4,*
BIOCELL, Vol.45, No.1, pp. 157-165, 2021, DOI:10.32604/biocell.2021.010261
Abstract Chimeric antigen receptor (CAR) T-cell therapy is mostly limited to hematological malignancies and has a poor effect on solid tumors. CAR T cells as a kind of immune cell may be affected by some immunomodulatory drugs such as pomalidomide, so the use of pomalidomide may improve the effect of CAR T cells on solid tumors. In this study, CD133- or HER2-specific CAR T cells were chosen to investigate whether pomalidomide can regulate the function of CAR T cells in vitro. We found that pomalidomide can significantly enhance the ability of CD133-CAR T cells and HER2-CAR T cells to kill tumor… More >

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ARTICLE

SUFANG ZHANG^1,2,#, XIANG LV^1,#, LI LI^3,#, YINGBIN LUO¹, HUINAN XIANG¹, LIXIN WANG^2,*, YAN LI^1,*
BIOCELL, Vol.45, No.1, pp. 167-175, 2021, DOI:10.32604/biocell.2021.013636
Abstract Chemotherapy is widely used for non-small cell lung cancer (NSCLC) patients at a late stage; however, NSCLC patients often acquire resistance to chemotherapeutic drugs, thus limiting the therapy efficacy. Melittin, a major component of bee venom, possesses anti-tumor activity in various cancer cells. Here, we examined the effects of melittin on A549/DDP cisplatin-resistant lung adenocarcinoma cells and xenografts formed from this cell line and investigated the possible target of melittin. Treatment with melittin resulted in the induction of cell apoptosis, glycolysis inhibition, and reduction of phosphorylated AKT (p-AKT) in A549/DDP cells. We also identified that tripartite motif-containing 8 (TRIM8) was… More >

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SINEM GÖKÇE KÜTÜK¹, MUSTAFA NAZIROĞLU^2,3,*
BIOCELL, Vol.45, No.1, pp. 177-188, 2021, DOI:10.32604/biocell.2021.013531
Abstract A protective action of melatonin (MELAT) on docetaxel (DCT)-induced inflammation, apoptosis, and reactive free oxygen radical (fROS) generation values via blocking of TRPM2 calcium-permeable channel was investigated in different cells except for laryngo-tracheal epithelial (LT-Epi) cells. Hence, the protective action of MELAT on DCT-induced oxidative toxicity and inflammation in LT-Epi tissue and cells of mice were investigated in the current study. MELAT treatment ameliorated DCTinduced mitochondrial ROS in the LT-Epi cells by reducing the generation of fROS (cytosolic and mitochondrial), lipid peroxidation, and depolarization of the mitochondrial membrane, while increasing reduced glutathione (GSH), GSH peroxidase, and total antioxidant status. In… More >

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YAO QIAN², JINGPING WANG², ZIWAN JI², HAO CHEN², YUCANG HE², XULING LV², ZIKAI ZHANG², TIAN LI², TIANYUN PAN³, LIQUN LI², MING LIN^1,*
BIOCELL, Vol.45, No.1, pp. 189-198, 2021, DOI:10.32604/biocell.2021.011827
Abstract The cell activity of adipose-derived stem cells (ADSCs) is affected by the intracellular reactive oxygen species (ROS) and the level of autophagy. Previous studies reveal that acetyl-L-carnitine (ALC) possesses capacities of resisting oxidative stress and regulating autophagy. Activating molecule in Beclin1-regulated autophagy protein 1 (AMBRA1) plays a key role in initiating Beclin1-regulated autophagy. In the present study, we discovered ALC pretreatment (1 mM, 24 h) significantly increased the activity of ADSCs exposed to H₂O₂ (100 μM, 2 h) in vitro with improved stemness, and reduced the production of intracellular ROS. In addition, we found for the first time that ALC… More >

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BEEMNET MENGESHA KASSAHUN^1,#, BEUM-CHANG KANG^2,#, SU-JI BAE², YE JIN NAM¹, GRETEL FONSECA MUNDO¹, GA-HUI KANG¹, KYOUNGOOK KIM³, JEUNG-SUL HAN^1,4,*
BIOCELL, Vol.45, No.1, pp. 199-215, 2021, DOI:10.32604/biocell.2021.012353
Abstract Solanum lycopersicum ‘Heinz 1706’ is a pioneer model cultivar for tomato research, whose whole genome sequence valuable for genomics studies is available. Nevertheless, a genetic transformation procedure for this cultivar has not yet been reported. Meanwhile, various genome editing technologies such as transfection of clustered regularly interspaced short palindromic repeats (CRISPR)/CRISPR-associated (Cas) ribonucleoprotein complexes into cells are in the limelight. Utilizing the Cas9-expressing genotype possessing a reference genome can simplify the verification of an off-target effect, resolve the economic cost of Cas9 endonuclease preparation, and avoid the complex assembly process together with single-guide RNA (sgRNA) in the transfection approach. Thus,… More >

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EDITORIAL

BIOCELL Editorial Office
BIOCELL, Vol.45, No.1, pp. 217-227, 2021, DOI:10.32604/biocell.2021.015666
Abstract This article has no abstract. More >

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