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Acetyl-L-carnitine protects adipose-derived stem cells exposed to H2O2 through regulating AMBRA1-related autophagy

YAO QIAN2, JINGPING WANG2, ZIWAN JI2, HAO CHEN2, YUCANG HE2, XULING LV2, ZIKAI ZHANG2, TIAN LI2, TIANYUN PAN3, LIQUN LI2, MING LIN1,*

1 The Second Affiliated Hospital and Yuying Children’s Hospital of Wenzhou Medical University, Wenzhou, 325000, China
2 The First Affiliated Hospital of Wenzhou Medical University, Wenzhou, 325000, China
3 Huzhou Hospital of Traditional Chinese Medicine, Huzhou, 313000, China

* Address correspondence to: Ming Lin, email

BIOCELL 2021, 45(1), 189-198. https://doi.org/10.32604/biocell.2021.011827

Abstract

The cell activity of adipose-derived stem cells (ADSCs) is affected by the intracellular reactive oxygen species (ROS) and the level of autophagy. Previous studies reveal that acetyl-L-carnitine (ALC) possesses capacities of resisting oxidative stress and regulating autophagy. Activating molecule in Beclin1-regulated autophagy protein 1 (AMBRA1) plays a key role in initiating Beclin1-regulated autophagy. In the present study, we discovered ALC pretreatment (1 mM, 24 h) significantly increased the activity of ADSCs exposed to H2O2 (100 μM, 2 h) in vitro with improved stemness, and reduced the production of intracellular ROS. In addition, we found for the first time that ALC treatment up-regulated autophagy of ADSCs through strengthening the expressions of Beclin1 and AMBRA1 synchronously, which might be involved in the protective effect of ALC.

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APA Style
QIAN, Y., WANG, J., JI, Z., CHEN, H., HE, Y. et al. (2021). Acetyl-l-carnitine protects adipose-derived stem cells exposed to h2o2 through regulating ambra1-related autophagy. BIOCELL, 45(1), 189-198. https://doi.org/10.32604/biocell.2021.011827
Vancouver Style
QIAN Y, WANG J, JI Z, CHEN H, HE Y, LV X, et al. Acetyl-l-carnitine protects adipose-derived stem cells exposed to h2o2 through regulating ambra1-related autophagy. BIOCELL . 2021;45(1):189-198 https://doi.org/10.32604/biocell.2021.011827
IEEE Style
Y. QIAN et al., “Acetyl-L-carnitine protects adipose-derived stem cells exposed to H2O2 through regulating AMBRA1-related autophagy,” BIOCELL , vol. 45, no. 1, pp. 189-198, 2021. https://doi.org/10.32604/biocell.2021.011827

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cc Copyright © 2021 The Author(s). Published by Tech Science Press.
This work is licensed under a Creative Commons Attribution 4.0 International License , which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
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