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ARTICLE
Nanoparticles induce the biosynthesis and activity of the new possible therapeutic proteinase source, Talaromyces purpureogenus KJ584844
SALLY NEGM1,2, MOHAMMAD EL-METWALLY3, WESAM ELDIN SABER4, SAHAR ABO-NEIMA5, MAHMOUD MOUSTAFA6,7,*, ATTALLA EL-KOTT6,8
1 Life Sciences Department, College of Science and literature Mahyel Aseer, King Khalid University, Abha, Saudi Arabia
2 Unit of Food Bacteriology, Central Laboratory of Food Hygiene, Ministry of Health, Sharkia, Egypt
3 Department of Botany and Microbiology, Faculty of Science, Damanhour University, El-Behera, Egypt
4 Microbial Activity Unit, Department of Microbiology, Soils, Water and Environment Research Institute, Agricultural Research Center, Giza, Egypt
5 Physics Department, Faculty of Science, Damanhour University, Damanhour, Egypt
6 Department of Biology, College of Science, King Khalid University, 9004, Abha, Saudi Arabia
7 Department of Botany and Microbiology, Faculty of Science, South Valley University, Qena, Egypt
8 Zoology Department, Faculty of Science, Damanhour University, El-Behera, Egypt
* Address correspondence to: Mahmoud MOUSTAFA,
(This article belongs to this Special Issue: New Insights in Biology of Depression: New Molecules and Approaches)
BIOCELL 2021, 45(1), 119-127. https://doi.org/10.32604/biocell.2021.012011
Received 10 June 2020; Accepted 22 August 2020; Issue published 26 January 2021
Abstract
The need for the bacterial proteinase is rapidly growing, urging to catch a lowcost medium for the microbial
fermentation, nanoparticles can play a vital role in this respect. The proteinase of
Talaromyces purpureogenus was
produced on the tubers of
Helianthus tuberosus that also operated as solid support for the fermentation process. The
interface amongst nitrogen sources (NH
4Cl and yeast extract) was investigated, applying the statistical modeling of
central composite design under solid-state fermentation. The optimum medium for proteinase secretion was
stimulated by 979.82 mg NH
4Cl and 437.68 mg yeast extract per 100 g substrate, yielding 108.15 U/g tubers. Using
Plackett-Burman experimental design, the nanoparticles Co, Ni and Fe were assessed as inducers for proteinase
stimulants. Co nanoparticles (5 ppm) were the greatest in both proteinase production by the fungus as well as an
inducer of the proteolysis process by the enzyme when using faba bean straw as a proteinaceous substrate in the
reaction mixture, liberating the extreme quantity of amino acids, compared with the lack of the nanoparticles. The
findings suggest the incorporation of Co nanoparticles in both the proteinase fabrication process and during
the degradation of proteinaceous materials induce proteinase catalyst. This approach could be extended to modulate
the productivity and activity of similar biomolecules.
Keywords
Cite This Article
NEGM, S., EL-METWALLY, M., SABER, W. E., ABO-NEIMA, S., MOUSTAFA, M. et al. (2021). Nanoparticles induce the biosynthesis and activity of the new possible therapeutic proteinase source,
Talaromyces purpureogenus KJ584844.
BIOCELL, 45(1), 119–127.
Citations