Home / Journals / BIOCELL / Vol.38, No.1, 2014
Table of Content
  • Open Access

    ARTICLE

    Antimalarial drug artesunate aff ords protection against carrageenan induced acute infl ammation in rat

    Vijay L. KUMAR1 *, B. GURUPRASAD1, Priyanka CHAUDHARY2
    BIOCELL, Vol.38, No.1, pp. 1-6, 2014, DOI:10.32604/biocell.2014.38.001
    Abstract Artesunate, an antimalarial drug, has been shown to inhibit the release of inflammatory mediators in various disease conditions. The present study was carried out to evaluate the anti-edematogenic effect of artesunate in the rat paw edema model. Inflammation was induced in the hind paw of rat by sub-plantar injection of 0.1 mL of 0.5% carrageenan and the paw volume was measured up to a fixed mark just before the injection and then after 3 h. The difference in two volumes gave a measure of edema formation. At 3h the level of TNF-α, PGE2 and myeloperoxidase were estimated in the inflamed… More >

  • Open Access

    ARTICLE

    Antiproliferative effect of extracts of Sida rhombifolia L. (Malvaceae) on the Allium cepa cell cycle

    Giselle de Moura CARPES, Viviane Dal-Souto FRESCURA*, Solange Bosio TEDESCO, Antonio Carlos Ferreira DA SILVA, Thais Scotti do CANTO-DOROW
    BIOCELL, Vol.38, No.1, pp. 7-10, 2014, DOI:10.32604/biocell.2014.38.007
    Abstract Field collected roots of four populations of Sida rhombifolia were used for preparing aqueous decoctions at two concentrations: 4g/L; and 16g/L. Afterwards, we used three groups of six onion (Allium cepa) bulbs for testing each population. Slides were made with all bulbs through the smashing technique. Cells in all phases of the cell cycle of A. cepa were analyzed. The mitotic index (% of cells in mitosis) was calculated, and the statistical analysis through the χ2 test was carried out at 5% probability. The results showed that the aqueous extracts of S. rhombifolia have antiproliferative activity at high concentrations. Practically… More >

  • Open Access

    ARTICLE

    Cytotoxicity assessment of a gold nanoparticle-chitosan nanocomposite as an effi cient support for cell immobilization: comparison with chitosan hydrogel and chitosan-gelatin

    Mohammad Reza RAMEZANI1, Hossein NADERI-MANESH1, *, Hossain-Ali RAFIEEPOUR2
    BIOCELL, Vol.38, No.1, pp. 11-16, 2014, DOI:10.32604/biocell.2014.38.011
    Abstract Cell-based biosensors have become a research hotspot in the biosensors and bioelectronics fields. The main feature of cell-based biosensors is immobilization of living cells on the surface of transducers. Different types of polymers which are used as scaffolds for cell growth should be biocompatible and should have reactive functional groups for further attachment of biomolecules. In this work, cell attachment and proliferation on chitosan hydrogel, chitosan-gelatin and gold nanoparticle-chitosan nanocomposite membranes was studied. Characterization of the membranes was performed using scanning electron microscopy (SEM) and Fourier transform infrared spectroscopy (FTIR). Cytotoxicity assessment on HEK293 cells was carried out for all… More >

  • Open Access

    ARTICLE

    Overexpression of inhibin α (1-32) fusion protein promotes apoptosis and cell cycle arrest in a cervical cancer cell model (Hela cells)

    Yanhong ZHEN1, Li HAN2, Kailai CAI1, Lijun HUO1, Hasan RIAZ1, Canjie WU1, Aixin LIANG1 , Lei SANG1, Liguo YANG1 *
    BIOCELL, Vol.38, No.1, pp. 17-24, 2014, DOI:10.32604/biocell.2014.38.017
    Abstract Inhibins play important roles in the reproductive system. To evaluate whether inhibin α (1-32) fusion protein plays a role in cervical cancer growth, the plasmid pVAX-inhα was constructed and its effect on proliferation and apoptosis of the human cervical cancer cell line (Hela) was checked by flow cytometry and real-time PCR. The expression and localization of inhibin α protein were detected by RT-PCR and confocal microscopy which showed that inhibin α protein was expressed and localized in the nucleus of Hela cells. Over expression of inhibin α gene significantly induced cell apoptosis and ceased S phase of cell cycle. Furthermore,… More >

  • Open Access

    ARTICLE

    Alanine minimises hepatocyte injury after ischemia-reperfusion in an ex vivo rat liver model

    Berengere PAPEGAY1, *, Michaela STADLER1, Vincent Nuyens1, Isabelle SALMON2, Veronique KRUYS3, Jean G. BOOGAERTS1
    BIOCELL, Vol.38, No.1, pp. 25-32, 2014, DOI:10.32604/biocell.2014.38.025
    Abstract Ischemia-reperfusion injury is a determinant in liver injury occurring during surgery, ischemic states and multiple organ failure. The pre-existing nutritional status of the liver, i.e., fasting, might contribute to the extent of tissue injury. This study investigated whether alanine, an amino acid precursor of glucose, could protect ex vivo perfused livers of fasting rats from reperfusion injury. The portal vein was cannulated, the liver removed and perfused in a closed ex vivo system. Isolated livers were perfused either with glucose 1 g/L and 10 g/L, or with equal concentrations of alanine (n = 10 in each group). The experiment consisted… More >

  • Open Access

    ARTICLE

    Meiotic behavior and pollinic viability in bean cultivars

    Gracieli DALLA NORA, Solange Bosio TEDESCO, Tamara PASTORI, Viviane Dal-Souto FRESCURA*, Nerinéia Dalfollo RIBEIRO, Haywood Dail LAUGHINGHOUSE IV, Sidinei José LOPES, Maria Teresa SCHIFINO-WITTMANN
    BIOCELL, Vol.38, No.1, pp. 33-38, 2014, DOI:10.32604/biocell.2014.38.033
    Abstract The aims of this study were to determine the meiotic behavior and to estimate pollen grains viability in bean (Phaseolus vulgaris L.) cultivars. Flower buds were collected during different developmental stages of the Mesoamerican bean cultivars IAPAR 44, Guapo Brilhante, BRS Expedito, BRS Valente, Guateian 6662 and Pérola, and the Andean bean cultivar Iraí, grown in a greenhouse. The meiotic index was determined by anther squashing of material fixed in absolute ethanol-glacial acetic acid (3:1) and stained with acetic orcein. No meiotic abnormalities were observed and the meiotic indices were high for all cultivars, indicating that the mismatch generated during… More >

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