Home / Journals / BIOCELL / Vol.30, No.1, 2006
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  • Open Access

    ARTICLE

    Argentinean cultivars of Vitis vinifera grow better than european ones when cultured in vitro under salinity

    JUAN B. CAVAGNARO, MARÍA T. PONCE, JAVIER GUZMÁN, MIGUEL A. CIRRINCIONE
    BIOCELL, Vol.30, No.1, pp. 1-7, 2006, DOI:10.32604/biocell.2006.30.001
    Abstract Argentinean Vitis vinifera cultivars although originated from Europe, have clear ampelographic and genotypic differences as compared with the European cultivars currently used in wine making. In vitro evaluation of salt tolerance has been used in many species. Our hypothesis was that Argentinean cultivars are more tolerant to salinity than European ones. Three European cultivars, Malbec, Cabernet Sauvignon and Chardonnay and four Argentinean cultivars, Cereza, Criolla Chica, Pedro Gimenez and Torrontes Riojano were tested by in vitro culture. Treatments included: 1) Control, 2) 60 mEq/L of a mixture of three parts of NaCl and one part of CaCl2 and 3) 90… More >

  • Open Access

    ARTICLE

    Evaluation of cytotoxic potential of latex of Calotropis procera and Podophyllotoxin in Allum cepa root model

    R. SEHGAL, S. ROY, V.L. KUMAR*
    BIOCELL, Vol.30, No.1, pp. 9-13, 2006, DOI:10.32604/biocell.2006.30.009
    Abstract In the present study we have utilized the Allium cepa root tip meristem model to evaluate the cytotoxic and anti-mitotic activities of latex of Calotropis procera (DL) and podophyllotoxin. Standard cytotoxic drug cyclophosphamide and non-cytotoxic drugs cyproheptadine and aspirin served as controls. Like cyclophosphamide, both DL and podophyllotoxin significantly inhibited the growth of roots and mitotic activity in a dose-dependent manner. However, podophyllotoxin was more potent in this regard and produced root decay. Cyproheptadine and aspirin, on the other hand, showed a marginal effect on the root growth and mitotic activity at much higher concentrations. More >

  • Open Access

    ARTICLE

    Genomic cloning and characterization of a PPA gene encoding a mannose-binding lectin from Pinellia pedatisecta

    JUAN LIN1, XUANWEI ZHOU2, JIONG FEI2, ZHIHUA LIAO1, WANG JIN1, XIAOFEN SUN1,*, KEXUAN TANG1,2,*
    BIOCELL, Vol.30, No.1, pp. 15-25, 2006, DOI:10.32604/biocell.2006.30.015
    Abstract A gene encoding a mannose-binding lectin, Pinellia pedatisecta agglutinin (PPA), was isolated from leaves of Pinellia pedatisecta using genomic walker technology. The ppa contained an 1140-bp 5’-upstream region, a 771-bp open reading frame (ORF) and an 829-bp 3’-downstream region. The ORF encoded a precursor polypeptide of 256 amino acid residues with a 24-amino acid signal peptide. There were one putative TATA box and six possible CAAT boxes lying in the 5’-upstream region of ppa. The ppa showed significant similarity at the nucleic acid level with genes encoding mannose-binding lectins from other Araceae species such as Pinellia ternata, Arisaema heterophyllum, Colocasia… More >

  • Open Access

    ARTICLE

    Visualization of the ribosomal DNA (45S rDNA) of Indica rice with FISH on some phases of cell cycle and extended DNA fibers

    ZONG-YUN LI1,2*, MEI-LI FU1, FANG-FANG HU1, SHU-FENG HUANG1, YUN-CHUN SONG2
    BIOCELL, Vol.30, No.1, pp. 27-32, 2006, DOI:10.32604/biocell.2006.30.027
    Abstract The ribosomal DNA (45S rDNA) behaviors during the cell cycle were analyzed on interphase nuclei, prophases, metaphases, pachytene chromosomes and extended DNA fibers in rice (Oryza,sativa ssp.indica cv.Guangluai No.4) by using high-resolution fluorescent in situ hybridization (FISH). The results show that 45S rDNA is located at the ends of short arms of chromosomes 9 and 10. But the signals are much more intense on chromosome 9 than on chromosome 10 in metaphase. Pachytene chromosome has rDNA signal arrays on chromosome 9. Different phases are described and discussed. These results indicate that the activity of rDNA at individual loci may also… More >

  • Open Access

    ARTICLE

    Structural and ultrastructural characterization of zebu (Bos indicus) spermatozoa

    MARIA CAROLINA AQUINO LUQUE, SÔNIA NAIR BÁO
    BIOCELL, Vol.30, No.1, pp. 33-38, 2006, DOI:10.32604/biocell.2006.30.033
    Abstract The ultrastructure of normal, ejaculated spermatozoa of Bos indicus was studied by means of electron microscopy, being evaluated in two principal parts, the head and the tail. The head is flat, oval or paddle-shaped with a square base, which provides a concave recess for the insertion of the tail. The acrosome tightly covers the anterior two thirds of the nucleus. A distinct unilateral acrosomal bulge was observed along the apical edge of the head. The equatorial region demarcates the acrosome from the post-equatorial region that covers the caudal one third of the nucleus. The classical 9+9+2 fiber pattern which composes… More >

  • Open Access

    ARTICLE

    Synergism of flavonoids with bacteriostatic action against Staphylococcus aureus ATCC 25 923 and Escherichia coli ATCC 25 922

    MARÍA DE LOS A. ALVAREZ, NORA B. DEBATTISTA, NORA B. PAPPANO
    BIOCELL, Vol.30, No.1, pp. 39-42, 2006, DOI:10.32604/biocell.2006.30.039
    Abstract In our previous studies, bacteriostatic action of flavonoids against Staphylococcus aureus ATCC 25 923 and Escherichia coli ATCC 25 922 was demostrated. In the present work synergism of their combinations in order to improve the bacteriostatic action against the same microorganisms was determined. The experiences were made in nutritive broth, maintaining constant one drug concentration (20 µg/ml) and increasing the other one. A turbidimetric kinetic method was used and by means of a mechanism previously proposed, the minimal inhibitory concentrations (MIC´s) of each flavonoid combination were determined. The MIC´s for assayed combinations against S. aureus were: variable morin - constant… More >

  • Open Access

    ARTICLE

    Single-channel response of hamster oocytes to fertilization with homologous spermatozoa

    LEONOR M.E. ITUARTE*, TERESA B. VIERA*, TEOBALDO A. SALDEÑA*, JUAN C. DE ROSAS**, MABEL FÓSCOLO**, JORGE E. IBÁÑEZ*, FERNANDO D. SARAVÍ*
    BIOCELL, Vol.30, No.1, pp. 43-49, 2006, DOI:10.32604/biocell.2006.30.043
    Abstract Electrophysiological events occur early after fertilization, along with changes in intracellular Ca2+ concentration . Passive electrical parameters were determined in golden hamster oocytes by whole cell patch-clamp method. In separate experiments the effect of 4-aminopyridine on resting oocytes was tested. The single-channel patch clamp configuration was employed to assess the electrical response to fertilization with homologous sperm. Structure of oocytes submitted to patch clamp was evaluated with scanning electron microscopy and found to be preserved.
    Oocyte diameter was 70.2 ± 2.2 µm; their resting parameters were: membrane potential 23.8 ± 0.8 mV; total membrane specific resistance 519.1 ± 94.6… More >

  • Open Access

    ARTICLE

    Herbicide effects on cuticle ultrastructure in Eleusine indica and Portulaca oleracea

    ROSANA N. MALPASSI
    BIOCELL, Vol.30, No.1, pp. 51-56, 2006, DOI:10.32604/biocell.2006.30.051
    Abstract Eleusine indica and Portulaca oleracea are two common weeds in peanut crops in southern Córdoba. Two chemicals are frequently used to control them, quizalofop for grasses and lactofen for dicots. The objective is to study the effects of quizalofop and lactofen on cuticle ultrastructure in E. indica and P. oleracea, respectively. In the lab, quizalofop was applied on E. indica and lactofen on P. oleracea. Three plant categories were analyzed in each species: 3, 1-2, and no tiller in E. indica, and 8, 6, and 2 nomophylls in P. oleracea. Leaf samples from both species were collected at 7 and… More >

  • Open Access

    ARTICLE

    Synaptonemal complexes and XY behavior in two species of argentinian armadillos: Chaetophractus villosus and Dasypus hybridus (Xenarthra, Dasypodidae)

    R. B. SCIURANO, M.S. MERANI, JIMENA BUSTOS, A.J. SOLARI
    BIOCELL, Vol.30, No.1, pp. 57-66, 2006, DOI:10.32604/biocell.2006.30.057
    Abstract Spermatocytes from the two armadillo species, C. villosus and D. hybridus were studied in microspreads for synaptonemal complexes (SCs) and in thin sections for electron microscopy (EM). The complete SC karyotype generally agrees with previous reports on mitotic chromosomes, except for the sex chromosomes. The X chromosome is submetacentric in both species and the Y is the shortest one in C. villosus and the second shortest in D. hybridus, and an extremely acrocentric one. A SC is formed along the total length of the Y chromosome, and this SC persists along all the pachytene substages. A single recombination nodule (RN)… More >

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