Table of Content

Open Access iconOpen Access

ARTICLE

Genomic cloning and characterization of a PPA gene encoding a mannose-binding lectin from Pinellia pedatisecta

JUAN LIN1, XUANWEI ZHOU2, JIONG FEI2, ZHIHUA LIAO1, WANG JIN1, XIAOFEN SUN1,*, KEXUAN TANG1,2,*

1. State Key Laboratory of Genetic Engineering, School of Life Sciences, Fudan-SJTU-Nottingham Plant Biotechnology R&D Center, Morgan-Tan International Center for Life Sciences, Fudan University, Shanghai 200433, People’s Republic of China;
2. Plant Biotechnology Research Center, Fudan-SJTU-Nottingham Plant Biotechnology R&D Center, School of Agriculture and Biology, Shanghai Jiaotong University, Shanghai 200030, People’s Republic of China;
Address correspondence to: Xiaofen Sun and Kexuan Tang. State Key Laboratory of Genetic Engineering, School of Life Sciences, Fudan University, Shanghai 200433, CHINA. Fax: (+86-21) 65643552. E-mail: xfsun1@sohu.com or kxtang1@yahoo.com or kxtang1@sohu.com

BIOCELL 2006, 30(1), 15-25. https://doi.org/10.32604/biocell.2006.30.015

Abstract

A gene encoding a mannose-binding lectin, Pinellia pedatisecta agglutinin (PPA), was isolated from leaves of Pinellia pedatisecta using genomic walker technology. The ppa contained an 1140-bp 5’-upstream region, a 771-bp open reading frame (ORF) and an 829-bp 3’-downstream region. The ORF encoded a precursor polypeptide of 256 amino acid residues with a 24-amino acid signal peptide. There were one putative TATA box and six possible CAAT boxes lying in the 5’-upstream region of ppa. The ppa showed significant similarity at the nucleic acid level with genes encoding mannose-binding lectins from other Araceae species such as Pinellia ternata, Arisaema heterophyllum, Colocasia esculenta and Arum maculatum. At the amino acid level, PPA also shared varying homology (ranging from 40% to 85%) with mannose-binding lectins from other plant species, such as those from Araceae, Alliaceae, Iridaceae, Lillaceae, Amaryllidaceae and Bromeliaceae. The cloning of the ppa gene not only provides a basis for further investigation of PPA’s structure, expression and regulation mechanism, but also enables us to test its potential role in controlling pests and fungal diseases by transferring the gene into tobacco and rice in the future.

Keywords


Cite This Article

APA Style
LIN, J., ZHOU, X., FEI, J., LIAO, Z., JIN, W. et al. (2006). Genomic cloning and characterization of a PPA gene encoding a mannose-binding lectin from <i>pinellia pedatisecta</i>. BIOCELL, 30(1), 15-25. https://doi.org/10.32604/biocell.2006.30.015
Vancouver Style
LIN J, ZHOU X, FEI J, LIAO Z, JIN W, SUN X, et al. Genomic cloning and characterization of a PPA gene encoding a mannose-binding lectin from <i>pinellia pedatisecta</i>. BIOCELL . 2006;30(1):15-25 https://doi.org/10.32604/biocell.2006.30.015
IEEE Style
J. LIN et al., "Genomic cloning and characterization of a PPA gene encoding a mannose-binding lectin from <i>Pinellia pedatisecta</i>," BIOCELL , vol. 30, no. 1, pp. 15-25. 2006. https://doi.org/10.32604/biocell.2006.30.015

Citations




cc This work is licensed under a Creative Commons Attribution 4.0 International License , which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
  • 1233

    View

  • 741

    Download

  • 0

    Like

Share Link