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  • Open Access

    REVIEW

    Research progress on natural products against hepatocellular carcinoma

    LINGLI ZHANG1,2,#, YAN LI1,#, JINGXIN MAO1,*
    BIOCELL, DOI:10.32604/biocell.2024.050396
    Abstract Hepatocellular carcinoma (HCC) remains a prevalent and challenging malignancy globally, characterized by its numerous causal factors and generally unfavorable prognosis. In the relentless pursuit of effective treatment modalities, natural products have emerged as a promising and relatively non-toxic alternative, garnering significant interest. The integration of natural products with contemporary medical research has yielded encouraging therapeutic outcomes in the management of HCC. This review offers a comprehensive overview of the causal factors underlying HCC, and the diverse treatment options available, and highlights the advancements made by natural products in anti-HCC research. Particularly, we provide an outline of the various types of… More >

  • Open Access

    ARTICLE

    Oleanolic acid inhibits colon cancer cell stemness and reverses chemoresistance by suppressing JAK2/STAT3 signaling pathway

    RUOYU CHEN1, YIMAN WU1, FENG WANG1, JUNTAO ZHOU1, HUAZHANG ZHUANG1, WEI LI2,*
    BIOCELL, DOI:10.32604/biocell.2024.051074
    Abstract Background: Oleanolic acid (OA), a pentacyclic triterpenoid exhibiting specific anti-cancer properties and highly effective antioxidant activity, was isolated from traditional Chinese medicinal herbs. Conversely, the OA that impacts colon cancer (CC) cells and its underlying mechanisms remain poorly understood. Methods: The cytotoxic effect of OA alone or OA-5-Fluorouracil (5-FU) combination on normal and CC cells was analyzed by methyl thiazolyl diphenyl-tetrazolium bromide (MTT). Then, the impact of OA on CC cell lines (LoVo and HT-29) proliferation and stemness were measured using colon formation and tumorsphere formation assays. Octamer-binding transcription factor 4 (Oct4), Prominin-1 (CD133), Nanog, and transcription factor SOX-2 (SOX2)… More >

  • Open Access

    ARTICLE

    Sciadopitysin exerts anticancer effects on HepG2 hepatocellular carcinoma cells by regulating reactive oxygen species-mediated signaling pathways

    YAN-NAN LI1,#, YUN-HONG XIU2,#, YAN-JUN TANG3, JING-LONG CAO1, WEN-SHUANG HOU1, AN-QI WANG1, TIAN-ZHU LI4,*, CHENG-HAO JIN1,3,5,*
    BIOCELL, DOI:10.32604/biocell.2024.050515
    (This article belongs to the Special Issue: Navigating the Interplay of Cancer, Autophagy, ER Stress, Cell Cycle and Apoptosis: Mechanisms, Therapies, and Future Directions)
    Abstract Objectives: Sciadopitysin (SP) is a flavonoid in Ginkgo biloba that exhibits various pharmacological activities. This study aimed to investigate its antitumor effects and the underlying molecular mechanism of SP in hepatocellular carcinoma (HCC) cells. Methods: Network pharmacology was used for target prediction analysis. Cell Counting Kit-8 (CCK-8) assay was used to test the cell viability. Flow cytometry was used to test the cell cycle distribution, apoptosis status, and reactive oxygen species (ROS) levels. Transwell and wound-healing assay was used to test the migration effect of SP on HepG2 cells. Western Blot assay was used to test the expression levels of… More >
    Graphic Abstract

    Sciadopitysin exerts anticancer effects on HepG2 hepatocellular carcinoma cells by regulating reactive oxygen species-mediated signaling pathways

  • Open Access

    REVIEW

    The pathogenesis of chronic subdural hematoma in the perspective of neomembrane formation and related mechanisms

    MINGYUE HUANG1,#, JUNFEI DAI1,#, XIANLIANG ZHONG2, JIN WANG2, JIANZHONG XU2, BO DU2,*
    BIOCELL, DOI:10.32604/biocell.2024.050097
    Abstract Chronic subdural hematoma (CSDH) is a disease characterized by capsuled blood products that progressively occupy the intracranial space, causing intracranial hypertension and compression in the brain. CSDH frequently occurs in all demographics, especially in the elderly, but the pathogenesis of CSDH remains unclear. In this review, we discuss the origin, development, and current treatment strategies of CSDH. For the first time, we analyzed the cellular and molecular compositions of hematoma membranes with a focus on neomembrane formation, a complex early-stage interactive event in hematoma pathogenesis. We hypothesize that in patients with CSDH, dural border cells (DBCs) might be induced to… More >

  • Open Access

    ARTICLE

    UBE2T mediates the stemness properties of breast cancer cells through the mTOR signaling pathway

    JIAWEI YIN1, YONGSHENG WANG2,3, GUANGWEI WEI4, MINGXIN WEN3,*
    BIOCELL, DOI:10.32604/biocell.2024.049349
    (This article belongs to the Special Issue: Cell Death and Inflammation in Signaling and Diseases)
    Abstract Objectives: This study aimed to reveal the role and possible mechanism of the ubiquitin-conjugating enzyme 2T (UBE2T) in the biological activities of breast cancer stem cells (BCSCs). Methods: The specific protein and gene expression were quantified by Western blotting and quantitative real-time polymerase chain reaction, the proportion of BCSCs was examined by flow cytometry, and the self-renewal and proliferation of BCSCs were verified by serial sphere formation and soft agar. Results: Increasing expression of UBE2T was drastically found in breast cancer than that in adjacent tissues. Furthermore, UBE2T overexpression significantly increased the proportion of BCSCs in breast cancer cells and… More >

  • Open Access

    REVIEW

    Molecular and cellular mechanisms of neuroprotection by oligopeptides from snake venoms

    CARLOS ALBERTO-SILVA*, BRENDA RUFINO DA SILVA
    BIOCELL, DOI:10.32604/biocell.2024.050443
    (This article belongs to the Special Issue: Exploring the Cellular Mechanisms of Neurodegenerative Diseases)
    Abstract Venom snake-derived peptides have multiple biochemical, pharmacological, and toxicological profiles, allowing for the discovery of new medicinal products and therapeutic applications. This review specifically examines the fundamental elements of neuroprotection offered by different oligopeptides derived from snake venom. It also includes a brief evaluation of short peptides that are being considered as potential therapeutic agents. Proline-rich peptides and tryptophyllin family peptides isolated from the crude venom of Viperidae family snakes, specifically Bothrops atrox, Bothrops jararaca, and Bothrops moojeni, have been shown to have pro-survival properties, the ability to reduce oxidative stress, and the ability to promote cell viability and mitochondrial… More >

  • Open Access

    ARTICLE

    Circular RNA circ_0003609 ameliorates hypertrophied ligamentum flavum by regulating the miR-155/SIRT1 axis

    GUIBIN ZHONG1,2,#, SHURONG WANG3,#, YUJIN HE4, DAMING FENG1, KE WEI1, YANQIU YANG1, JIANWEI CHEN1,2,*, JUNLING CHEN1,*
    BIOCELL, DOI:10.32604/biocell.2024.050294
    (This article belongs to the Special Issue: MicroRNA as Biomarkers for Disease Diagnosis and Progression)
    Abstract Background: Hypertrophy of the ligamentum flavum (HLF) is a common contributor to spinal stenosis which results in significant neurological impairments. Circular RNA (circRNA) circ_0003609 has been linked to HLF; however, the exact mechanism by which it causes this disease is unclear. Methods: Circ_0003609 expressions were regulated in HLF cells by overexpression vectors and RNA interference. Cell proliferation and fibrosis-related gene expression were checked by the Cell Counting Kit-8 (CCK-8) assay and western blotting. CircBank’s prediction of the association between miR-155 and circ_0003609 was supported by a dual-luciferase reporter experiment. The function of the miR- 155/sirtuin 1 (SIRT1) axis in controlling… More >

  • Open Access

    ARTICLE

    Bhlhe40 protects cochlear hair cell-like HEI-OC1 cells against HO‑triggered oxidative injury

    LITING WEN#, XIAOXIA ZENG#, PEIXIONG CHEN, DAPENG ZHAO, YANGYANG LI, XIANHAI ZENG*
    BIOCELL, DOI:10.32604/biocell.2024.050219
    Abstract Background: Cochlear hair cell injury is a common pathological feature of hearing loss. The basic helix-loop-helix family, member e40 (Bhlhe40), a gene belonging to the basic helix-loop-helix (bHLH) family, exhibits strong transcriptional repression activity. Methods: Oxidative damage, in House Ear Institute-Organ of Corti 1 (HEI‑OC1) cells, was caused using hydrogen peroxide (HO). The Ad-Bhlhe40 particles were constructed to overexpress Bhlhe40 in HEI-OC1 cells. Various assays including cell counting kit-8 (CCK-8), terminal deoxynucleotidyl transferase-mediated dUTP nick end-labeling assay (TUNEL), flow cytometry, immunofluorescence, and corresponding commercial kits were employed to investigate the impacts of Bhlhe40 on cell viability, apoptosis, oxidative stress levels,… More >
    Graphic Abstract

    <i>Bhlhe40</i> protects cochlear hair cell-like HEI-OC1 cells against HO‑triggered oxidative injury

  • Open Access

    ARTICLE

    miR-557 suppresses hepatocellular carcinoma cell proliferation and migration via downregulating CBX4

    XULONG SUN1,#, WENTAO DING2,#, CHAO JIANG3, ZHIAN FANG4,*
    BIOCELL, DOI:10.32604/biocell.2024.050519
    (This article belongs to the Special Issue: New Perspectives on Inflammatory Cancer Transformation)
    Abstract Introduction: Hepatocellular carcinoma (HCC), a prevalent malignancy, poses significant challenges with high tumor heterogeneity and poor prognosis. MicroRNAs (miRNAs) play a pivotal role in hepatocarcinogenesis. Although abnormalities in microRNA-557 (miR-557) expression have been implicated in various cancer types, its role in HCC remains unclear. Therefore, there is a need to explore the function of microRNA-557 in HCC. Methods: Candidate miRNAs were identified through screening in GSE108724 and GSE20077. Real-time PCR was employed to analyze the expression level of miR-557 in hepatoma cell lines and tissues. Cell viability and migration assays were applied to assess the impact of miR-557 on HCC… More >
    Graphic Abstract

    miR-557 suppresses hepatocellular carcinoma cell proliferation and migration via downregulating CBX4

  • Open Access

    REVIEW

    Computational and bioinformatics tools for understanding disease mechanisms

    MOHD ATHAR1,*, ANU MANHAS2, NISARG RANA2, AHMAD IRFAN3
    BIOCELL, DOI:10.32604/biocell.2024.049891
    (This article belongs to the Special Issue: Computational Approaches in Molecular and Cellular Biology: Advancements in Disease Research and Therapeutic Design)
    Abstract Computational methods have significantly transformed biomedical research, offering a comprehensive exploration of disease mechanisms and molecular protein functions. This article reviews a spectrum of computational tools and network analysis databases that play a crucial role in identifying potential interactions and signaling networks contributing to the onset of disease states. The utilization of protein/gene interaction and genetic variation databases, coupled with pathway analysis can facilitate the identification of potential drug targets. By bridging the gap between molecular-level information and disease understanding, this review contributes insights into the impactful utilization of computational methods, paving the way for targeted interventions and therapeutic advancements… More >

  • Open Access

    ARTICLE

    Revealing the role of honokiol in human glioma cells by RNA-seq analysis

    YUNBAO GUO1,#, XU LIU1,#, QI XU2, XIAOTONG ZHOU3, JIAWEI LIU3, YANYAN XU2, YAN LU2,*, HAIYAN LIU2,*
    BIOCELL, DOI:10.32604/biocell.2024.049748
    Abstract Background: Glioma is a kind of tumor that easily deteriorates and originates from glial cells in nerve tissue. Honokiol is a bisphenol compound that is an essential monomeric compound extracted from the roots and bark of Magnoliaceae plants. It also has anti-infection, antitumor, and immunomodulatory effects. In this study, we found that honokiol induces cell apoptosis in the human glioma cell lines U87-MG and U251-MG. However, the mechanism through which honokiol regulates glioma cell apoptosis is still unknown. Methods: We performed RNA-seq analysis of U251-MG cells treated with honokiol and control cells. Protein-protein interaction (PPI) network analysis was performed, and… More >
    Graphic Abstract

    Revealing the role of honokiol in human glioma cells by RNA-seq analysis

  • Open Access

    ARTICLE

    OPA3 overexpression modulates lipid droplet production and sensitizes colorectal cancer cells to bevacizumab treatment

    HONGBIAO WU*, DONGFANG LIU
    BIOCELL, DOI:10.32604/biocell.2024.049466
    (This article belongs to the Special Issue: Intestinal Epithelial Cells in Health and Disease)
    Abstract Background: Colorectal cancer (CRC) represents a substantial risk to public health. Bevacizumab, the first US FDA-approved antiangiogenic drug (AAD) for human CRC treatment, faces resistance in patients. The role of lipid metabolism, particularly through OPA3-regulated lipid droplet production, in overcoming this resistance is under investigation. Methods: The protein expression pattern of OPA3 in CRC primary/normal tissues was evaluated by bioinformatics analysis. OPA3-overexpressed SW-480 and HCT-116 cell lines were established, and bevacizumab resistance and OPA3 effects on cell malignancy were examined. OPA3 protein/mRNA expression and lipid droplet-related genes were measured with Western blot and qRT-PCR. OPA3 subcellular localization was detected using… More >
    Graphic Abstract

    OPA3 overexpression modulates lipid droplet production and sensitizes colorectal cancer cells to bevacizumab treatment

  • Open Access

    ARTICLE

    Paclitaxel induces human KOSC3 oral cancer cell apoptosis through caspase pathways

    YU-YAN LAN1,#, TSUN-CHIH CHENG2,#, YI-PING LEE3, CHIA-YIH WANG3,*, BU-MIIN HUANG3,4,*
    BIOCELL, DOI:10.32604/biocell.2024.050701
    (This article belongs to the Special Issue: Navigating the Interplay of Cancer, Autophagy, ER Stress, Cell Cycle and Apoptosis: Mechanisms, Therapies, and Future Directions)
    Abstract Background: Paclitaxel is a compound derived from Pacific yew bark that induces various cancer cell apoptosis. However, whether it also has anticancer activities in KOSC3 cells, an oral cancer cell line, is unclear. Methods: 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide, flow cytometry, and western blotting assays were carried out to assess cell viability, subG1 phase of the cell cycle, and apoptosis-related protein expression, respectively. Results: Our findings indicate that paclitaxel could inhibit cell viability and increase the expression of apoptotic markers, including plasma membrane blebbing and the cleavage of poly ADP-ribose polymerase in KOSC3 cells. Also, the treatment with paclitaxel remarkably elevated the percentage… More >
    Graphic Abstract

    Paclitaxel induces human KOSC3 oral cancer cell apoptosis through caspase pathways

  • Open Access

    REVIEW

    Does young feces make the elderly live better? Application of fecal microbiota transplantation in healthy aging

    YUANYUAN LIAO1,2,3, XINSI LI2,3, QIAN LI2,3, YIZHONG WANG4, XIUJUN TAN1,2,3, TING GONG2,3,5,*
    BIOCELL, DOI:10.32604/biocell.2024.050324
    (This article belongs to the Special Issue: Gut Microbiota in Human Health: Exploring the Complex Interplay)
    Abstract As we are facing an aging society, anti-aging strategies have been pursued to reduce the negative impacts of aging and increase the health span of human beings. Gut microbiota has become a key factor in the anti-aging process. Modulation of gut microbiota by fecal microbiota transplantation (FMT) to prevent frailty and unhealthy aging has been a hot topic of research. This narrative review summarizes the benefits of FMT for health span and lifespan, brains, eyes, productive systems, bones, and others. The mechanisms of FMT in improving healthy aging are discussed. The increased beneficial bacteria and decreased pathological bacteria decreased gut… More >

  • Open Access

    REVIEW

    Non-coding RNA as future target for diagnose and treatment of perineural invasion in cancers

    BINGJIE LI1,#, WENBO CAO1,2,3,#, JINJING XIAO1, YIXIAO CHEN1, QIYING WEI1, MINGJIN YUE4, SAIJUN MO1,2,3,*
    BIOCELL, DOI:10.32604/biocell.2024.049160
    (This article belongs to the Special Issue: Advances in Biomarker Research: Unveiling the Pathways to Precision Medicine)
    Abstract Perineural invasion (PNI), a particularly insidious form of tumor metastasis distinct from hematogenous or lymphatic spread, has the capacity to extend well beyond the primary tumor site, infiltrating distant regions devoid of lymphatic or vascular structures. PNI often heralds a decrease in patient survival rates and is recognized as an indicator of an unfavorable prognosis across a variety of cancers. Despite its clinical significance, the underlying molecular mechanisms of PNI remain elusive, complicating the development of specific and efficacious diagnostic and therapeutic strategies. In the realm of cancer research, non-coding RNAs (ncRNAs) have attracted considerable attention due to their multifaceted… More >