Home / Journals / BIOCELL / Vol.29, No.1, 2005
Table of Content
  • Open Access

    ARTICLE

    Comparative study of mandibular glands of Melipona bicolor queens obtained from polygynic and monogynic colonies

    LUCIANA FIORETTI GRACIOLI-VITTI, FÁBIO CAMARGO ABDALLA, REGINA LÚCIA MORELLI SILVA DE MORAES
    BIOCELL, Vol.29, No.1, pp. 1-5, 2005, DOI:10.32604/biocell.2005.29.001
    Abstract The aim of the present study was analyze, by histological and morphometrical studies, mandibular glands of Melipona bicolor queens collected from monogynic and polygynic colonies and compare their level of development. The results showed that the glands of physogastric queens from monogynic colony present a higher level of activity in relation to the queens of polygynic colonies; this is explained by the fact that just a unique queen controls the monogynic colony. In the polygynic colonies, the queens may divide such control to each other. More >

  • Open Access

    ARTICLE

    Effects of filgrastim on granulopoietic cells of mice pretreated with methotrexate

    LILIAN BARRIOS, OSCAR HÉCTOR POLETTI
    BIOCELL, Vol.29, No.1, pp. 7-14, 2005, DOI:10.32604/biocell.2005.29.007
    Abstract We have evaluated the effect of filgrastim on proliferation and differentiation activity of granulopoietic cells in mice pretreated with methotrexate. Filgrastim was injected daily, from day 8 to 28 after cytotoxic agent administration. The granulopoiesis changes were measured by assessment of GM-CFU cells content, marrow and spleen granuloid cells pool as well as circulating neutrophils. In MTX pretreated mice, bone marrow GM-CFU oscillating values were higher than normal values, but these changes were not followed by high proliferative activity in granuloid precursor cell compartment. After MTX treatment, filgrastim administration was unable to stimulate marrow granulopoiesis as observed in normal mice.… More >

  • Open Access

    ARTICLE

    Fine structural analysis of the epithelial cells in the hepatopancreas of Palaemonetes argentinus (Crustacea, Decapoda, Caridea) in intermoult

    LILIANA G. SOUSA1, ELENA I. CUARTAS2, ANA MARÍA PETRIELLA1, 3
    BIOCELL, Vol.29, No.1, pp. 25-31, 2005, DOI:10.32604/biocell.2005.29.025
    Abstract The aim of this study is to describe the ultrastructure of the hepatopancreas of P. argentinus in intermoult. P. argentinus hepatopancreas was studied using standard TEM techniques. Each tubule consists of four cellular types: E (embryonic), F (fibrillar), R (resorptive) and B (blister like). E-cells have embryonic features and some of them were found in mitosis. F, R and B cells possess an apical brush border. F-cells have a central or basal nucleus, a conspicuous RER, and dilated Golgi cisternae. R cells show a polar organization of organelles in three areas: apical, with numerous mitochondria and sER tubules, a central… More >

  • Open Access

    ARTICLE

    Comparative study of DNA synthesis and nucleolar organizer regions of sinusoid littoral cells in mouse regenerating liver

    CARLOS A. MARTÍN, JOSÉ MIGUEL SURUR, MARCELA N. GARCÍA, FÉLIX CORRONS, AMADO F. BADRÁN
    BIOCELL, Vol.29, No.1, pp. 33-38, 2005, DOI:10.32604/biocell.2005.29.033
    Abstract Variations in DNA synthesis (DNAs) and Nucleolar Organizer Regions (NORs) were studied in the littoral cell population from regenerating liver of C3HS inbred mice standardized for periodicity analysis. Immunohistochemical detection of Bromodeoxyuridine (BrdU) with a monoclonal antibody and silver staining of NORs (AgNORs) were assessed by means of a digital image analysis system in histological sections. Tissue samples were obtained every four hours from the 30th to the 54th hours after a partial hepatectomy. The results showed, in both parameters, a gradual increment of the values during the period studied, with highest values (DNAs 107.1 ± 16.1 SE; AgNORs 77.3… More >

  • Open Access

    ARTICLE

    Morphoquantitative evaluation of the duodenal myenteric neuronal population in rats fed with hypoproteic ration

    MARIA RAQUEL MARÇAL NATALI, SONIA LUCY MOLINARI, LUIZ CRISTIANO VALENTINI, MARCÍLIO HUBNER DE MIRANDA NETO.
    BIOCELL, Vol.29, No.1, pp. 39-46, 2005, DOI:10.32604/biocell.2005.29.039
    Abstract The purpose of this work was to analyze the morphoquantitative behavior of the neurons of the myenteric plexus, as well as the morphometry of the duodenal wall, in adult rats fed with normoproteic (22%) and hypoproteic (8%) rations, killed at the age of 345 days. For neuronal assessments duodenal wholemounts stained with the Giemsa method were used, and for the evaluation of the duodenal wall routine histological processing and staining with Hematoxilin-Eosin were employed. The means of the number of neurons in 80 microscopic fields (12.72 mm2 ) and of the size of the neuronal cell bodies did not reveal… More >

  • Open Access

    ARTICLE

    Extracellular matrix of ostrich articular cartilage

    TATIANA CARLA TOMIOSSO, LAURECIR GOMES, BENEDICTO DE CAMPOS VIDAL, EDSON ROSA PIMENTEL
    BIOCELL, Vol.29, No.1, pp. 47-54, 2005, DOI:10.32604/biocell.2005.29.047
    Abstract The composition and organization of the extracellular matrix of ostrich articular cartilage was investigated, using samples from the proximal and distal surfaces of the tarsometatarsus. For morphological analysis, sections were stained with toluidine blue and analyzed by polarized light microscopy. For biochemical analysis, extracellular matrix components were extracted with 4 M guanidinium chloride, fractionated on DEAE-Sephacel and analyzed by SDS-PAGE. Glycosaminoglycans were analyzed by electrophoresis in agarose gels. Structural analysis showed that the fibrils were arranged in different directions, especially on the distal surface. The protein and glycosaminoglycan contents of this region were higher than in the other regions. SDS-PAGE… More >

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