Home / Journals / BIOCELL / Vol.45, No.4, 2021
Table of Content
  • Open Access

    REVIEW

    Microfluidic chips for the endothelial biomechanics and mechanobiology of the vascular system

    HAORAN SU1, KEXIN LI1, XIAO LIU1,*, JING DU1, LI WANG4, XIAOYAN DENG3,*, YUBO FAN1,2,*
    BIOCELL, Vol.45, No.4, pp. 797-811, 2021, DOI:10.32604/biocell.2021.014900
    (This article belongs to this Special Issue: Cellular Biomechanics in Health and Diseases)
    Abstract Endothelial cells arranged on the vessel lumen are constantly stimulated by blood flow, blood pressure and pressureinduced cyclic stretch. These stimuli are sensed through mechanical sensory structures and converted into a series of functional responses through mechanotransduction pathways. The process will eventually affect vascular health. Therefore, there has been an urgent need to establish in vitro endothelial biomechanics and mechanobiology of models, which reproduce three-dimensional structure vascular system. In recent years, the rapid development in microfluidic technology makes it possible to replicate the key structural and functionally biomechanical characteristics of vessels. Here, we summarized the progress of microfluidic chips used… More >

  • Open Access

    REVIEW

    Oxidative metabolism of photosynthetic species and the exposure to some freshwater and marine biotoxins

    SUSANA PUNTARULO1,2, PAULA MARIELA GONZÁLEZ1,2,*
    BIOCELL, Vol.45, No.4, pp. 813-821, 2021, DOI:10.32604/biocell.2021.015360
    (This article belongs to this Special Issue: Oxidative Stress in Aquatic Organisms)
    Abstract Environmental climate conditions could lead to an increasing global occurrence of microorganism blooms that synthesize toxins in the aquatic environments. These blooms could result in significantly toxic events. Responses of photosynthetic organisms to adverse environmental conditions implicate reactive oxygen species generation; but, due to the presence of a varied cellular antioxidant defense system and complex signaling networks, this oxidative stress could act as an important factor in the environmental adaptive processes. The objective of this review was to assess how some biotoxins are implicated in the generation of oxidative and nitrosative metabolic changes, not only in biotoxin-producing organisms but also… More >

  • Open Access

    REVIEW

    Cell cycle regulation through primary cilium: A long-forgotten story

    LIN LIU, ZHOUWEN XU, YUYAN JIANG, MD REZAUL KARIM, XIAO HUANG*
    BIOCELL, Vol.45, No.4, pp. 823-833, 2021, DOI:10.32604/biocell.2021.013864
    (This article belongs to this Special Issue: Kinesin Motors and Cell Cycle)
    Abstract Protruded from cytomembrane, primary cilium is a widespread cell organelle that can be found in almost all cell types in Mammalia. Because of its comprehensive requirement in various cellular activities and various functions in different organs, primary cilium has been a valuable research area of human pathology research since the turn of the millennium. And the potential application of the interaction between primary cilia and cell cycle regulation may be the most promising direction as many primary cilium-caused diseases are found to be caused by cell cycle dysregulation resulted from primary cilia defects. Therefore, a deep understanding of the interaction… More >

  • Open Access

    REVIEW

    Advances in molecular regulation of goat lipid metabolism and FAS structure and function regulation

    JIAHE GUO2,#, XIAOGUANG JI3,#, YONGJIANG MAO2, ZHANGPING YANG2, ZHI CHEN2, YUAN YUAN1,*
    BIOCELL, Vol.45, No.4, pp. 835-847, 2021, DOI:10.32604/biocell.2021.015652
    (This article belongs to this Special Issue: Decoding Gene (including circRNA, lincRNA miRNA and mRNA) Expression)
    Abstract Goat milk is widely recognized for its nutritional value. Fatty acid synthase (FAS) is the crucial enzyme of fatty acid de novo synthesis. It plays an important role in the formation of goat milk fat. In this paper, we first introduced the molecular regulation process of goat milk fat metabolism based on the structure research of FAS. Secondly, we reviewed some key factors in FAS transcription and post-transcriptional regulation of the goat mammary gland and preliminarily constructed the expression network of the goat mammary gland FAS gene. The purpose of this paper is to systematically introduce the role of FAS… More >

  • Open Access

    REVIEW

    Biomedical overview of melanin. 1. Updating melanin biology and chemistry, physico-chemical properties, melanoma tumors, and photothermal therapy

    ALFONSO BLÁZQUEZ-CASTRO1,2,*, JUAN CARLOS STOCKERT2,3
    BIOCELL, Vol.45, No.4, pp. 849-862, 2021, DOI:10.32604/biocell.2021.015900
    Abstract Melanins (eumelanin, pheomelanin, and allomelanin) represent a very, if not the most, important group of biological pigments. Their biological roles are multiple, from photoprotection to antioxidant activity, heavy metal disposal or the myriad uses of color in organisms across all Phyla. In the first part of this review, eumelanin biology and some chemical aspects will be presented, as well as key physico-chemical features that make this biological pigment so interesting. The principal characteristics of the melanocyte, the melanin-synthesizing cell in mammals, will also be introduced. Transformed melanocytes are the cause of one of the most devastating known cancers: the malignant… More >

  • Open Access

    REVIEW

    Mechanisms adopted by cancer cells to escape apoptosis–A review

    SAYEQUA DANDOTI*
    BIOCELL, Vol.45, No.4, pp. 863-884, 2021, DOI:10.32604/biocell.2021.013993
    Abstract Inactivation of apoptosis is the prime phenomenon in cancer development and cancer treatments. Mutations in the apoptotic pathway not only exert resistance to apoptosis and provide a survival advantage to cancer cells but also confer resistance to cancer therapies. Escaping apoptosis is the “hallmark” of cancer cells. Cancer cells can withstand many apoptotic stimuli, such as DNA damage, unfavorable environments, and cytotoxic therapies. Substantial research has been carried out and is in good progress on the various mechanisms adopted by cancer cells to evade apoptosis. This article reviews the apoptosis escape mechanisms by cancer cells, viz. apoptotic gene alterations (in… More >

  • Open Access

    ARTICLE

    Integrated analysis of human influenza A (H1N1) virus infectionrelated genes to construct a suitable diagnostic model

    WENBIAO CHEN, KEFAN BI, JINGJING JIANG, XUJUN ZHANG, HONGYAN DIAO*
    BIOCELL, Vol.45, No.4, pp. 885-899, 2021, DOI:10.32604/biocell.2021.012938
    Abstract The genome characteristics and structural functions of coding proteins correlate with the genetic diversity of the H1N1 virus, which aids in the understanding of its underlying pathogenic mechanism. In this study, analyses of the characteristic of the H1N1 virus infection-related genes, their biological functions, and infection-related reversal drugs were performed. Additionally, we used multi-dimensional bioinformatics analysis to identify the key genes and then used these to construct a diagnostic model for the H1N1 virus infection. There was a total of 169 differently expressed genes in the samples between 21 h before infection and 77 h after infection. They were used… More >

  • Open Access

    ARTICLE

    Circular RNA circFOXM1 triggers the tumorigenesis of non-small cell lung cancer through miR-132-3p/TMEM14A axis

    WEIGAO ZHONG1,*, AIQIN CHEN2, XIAOHONG TANG1, YI LIU1
    BIOCELL, Vol.45, No.4, pp. 901-910, 2021, DOI:10.32604/biocell.2021.08672
    Abstract Earlier studies indicated that circular RNAs (circRNAs) were found in various cancer cells, and circFOXM1 was reported to act as an oncogene in non-small cell lung cancer (NSCLC). However, the function of circFOXM1 in NSCLC remains unclear. The expression levels of genes were measured using quantitative real-time polymerase chain reactions (qRT-PCR). Cell proliferation and apoptosis were determined by 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide solution (MTT) and flow cytometry assay. The relative protein expression was assessed by western blot. Moreover, transwell assays were employed to examine cell migration and invasion. The targeted relationship was confirmed by dual-luciferase reporter assay. The expression of circFOXM1 was… More >

  • Open Access

    ARTICLE

    The F5 gene predicts poor prognosis of patients with gastric cancer by promoting cell migration identified using a weighted gene co-expression network analysis

    MENGYI TANG1,2,3,4,#, BOWEN YANG1,2,3,4,#, CHUANG ZHANG1,2,3,4, CHAOXU ZHANG1,2,3,4, DAN ZANG1,2,3,4, LIBAO GONG1,2,3,4, YUNPENG LIU1,2,3,4, ZHI LI1,2,3,4,*, XIUJUAN QU1,2,3,4,*
    BIOCELL, Vol.45, No.4, pp. 911-921, 2021, DOI:10.32604/biocell.2021.010119
    Abstract Distal gastric cancer (DGC) is a subgroup of gastric cancer (GC), which has different molecular characteristics from proximal gastric cancer (PGC). These differences result in different overall survival (OS) rates; however, data pertaining to the survival rate in PGC or DGC are contradictory. This suggests that the location of GC is not the unique cause of the different survival rates, while the molecular characteristics might be more important factors determining the prognosis of DGC. Therefore, the aim of this study was to discover key prognostic factors in DGC using bioinformatic methods and to explore the potential molecular mechanism. The Cancer… More >

  • Open Access

    ARTICLE

    Immunocytochemical examination of PTEN and Ki-67 for endometrial carcinoma using thin-layer endometrial preparations

    XI YANG1, KE MA1,*, RUI CHEN1, NAIYI ZHANG2, YITING MENG3, JIA WEN1, QINPING LIAO1
    BIOCELL, Vol.45, No.4, pp. 923-932, 2021, DOI: 10.32604/biocell.2021.013625
    Abstract The current study is designed to evaluate certain immunocytochemical (ICC) biomarkers to gain a better cytodiagnosis. For this purpose, 85 patients from March 2016 to March 2019 who planned to get a hysteroscopy assay were recruited. Cytological sampling was conducted by scratching the uterus cavity using SAP-1, and the samples were processed as liquid-based smears, using SurePath technology. 36 patients diagnosed with EC or atypical endometrial hyperplasia were recruited in this study. 33 cases were diagnosed with EC, and 3 cases were diagnosed with atypical endometrial hyperplasia, allocated with EC or precancerous lesions group. 26 cases were diagnosed with benign… More >

  • Open Access

    ARTICLE

    SENEX gene promotes cell proliferation by activating RB/E2F pathway in diffuse large B-cell lymphoma cells

    JIYU WANG#, YING PAN#, YANG WAN, ZHIXIANG WANYAN, ZHITAO WANG, QIANSHAN TAO, ZHIMIN ZHAI*
    BIOCELL, Vol.45, No.4, pp. 933-942, 2021, DOI:10.32604/biocell.2021.014280
    Abstract The present study aimed to clarify the role of SENEX in malignant cell proliferation in diffuse large B-cell lymphoma (DLBCL). 22 DLBCL patients (6 newly diagnosed cases, 7 cases at complete remission, and 9 relapsed cases) were included in the study. Our results indicated that both SENEX gene and protein were significantly increased in peripheral blood mononuclear cells (PBMCs) and tumor cells of relapsed DLBCL patients, accompanied by overexpression of p21, p16, and phosphorylated retinoblastoma (Rb). Silencing the SENEX gene in a DLBCL cell line caused a significant decrease in cell proliferation and inhibited cell cycle progression in the G1… More >

  • Open Access

    ARTICLE

    Hellebrigenin induces apoptosis in colorectal cancer Cells through induction of excessive reactive oxygen species

    CHUNJIAO LIU1,2, QINHONG KONG1,2, FENG PAN1,2, SHAN JIANG1,2, LINGJIE MENG1,2, GAI HUANG1,2, LIDAN LU1,2, SANHUA LI1,2,*, YUN LIU1,2,3,*
    BIOCELL, Vol.45, No.4, pp. 943-951, 2021, DOI:10.32604/biocell.2021.015836
    Abstract Traditional Chinese medicine (TCM) has been increasingly applied in both preventing and treating a variety of cancers in the last decades, attributing to its fewer side effects as compared with chemotherapy drugs. Hellebrigenin, a component of Chanpi from the skin of Bufo bufogargarizans Cantor or Duttaphrynus melanostictus has been reported to have an obvious anti-cancer activity on various cancers. However, the effect and mechanism of hellebrigenin on colorectal cancers were still unknown. Herein, the present study demonstrated hellebrigenin significantly reduced viability and triggered apoptosis via the intrinsic pathway in colorectal cancer cell lines HCT116 and HT29 in vitro and in… More >

  • Open Access

    ARTICLE

    PPARγ LBD and its ligand specificity reveal a selection of potential partial agonist: Molecular dynamics based T2D drug discovery initiative

    BIDYUT MALLICK1,#, ASHISH RANJAN SHARMA2,#, MANOJIT BHATTACHARYA3, SANG-SOO LEE1,*, CHIRANJIB CHAKRABORTY4,*
    BIOCELL, Vol.45, No.4, pp. 953-961, 2021, DOI:10.32604/biocell.2021.015530
    Abstract PPARγ is a peroxisome proliferator-activated receptor (PPAR) family protein and is a target for type 2 diabetes (T2D). In this paper, we have performed a molecular docking analysis between ligand molecules (CID9816265, CID11608015, CID20251380, CID20251343, CID20556263, CID624491, CID42609928, and CID86287562) and PPARγ to determine the ligand specificity. It also helps to understand the ligand-binding domain (LBD) activity of PPARγ during the binding of the ligand. Further, a molecular dynamics simulation study was performed to determine the ligand biding stability in the PPARγ LBD. Its ligand specificity informed us about the potentiality of selecting a partial agonist. The study also shows… More >

  • Open Access

    ARTICLE

    SOD1G93A Induces a Unique PSAP-Dependent Mitochondrial Apoptosis Pathway via Bax–Bak Interaction

    HAN NIU1,#, XIN CHEN1,#, XUEQI FU1, JINGTIAN ZHANG1, GUODONG LI4, YUXIANG WANG1, JIAYUE SONG1, XUETING MA1, CHEN HU5, XUEMIN XU3, FUQIANG ZHANG2,*, LINLIN ZENG1,*
    BIOCELL, Vol.45, No.4, pp. 963-970, 2021, DOI:10.32604/biocell.2021.015297
    Abstract Amyotrophic lateral syndrome (ALS) is a progressive degenerative disorder characterized by motor neuron death and axon degeneration. Mitochondrial dysfunction plays a key role in the pathogenesis of ALS, the mechanism of which remains poorly understood. The B-cell lymphoma-2 (Bcl-2) family of proteins that control and mediate mitochondrial function and apoptosis, including the pro-apoptotic members Bcl2-Associated X (Bax), are involved in ALS development. The death receptor 6 (DR6) regulates motor neuron death in ALS, and DR6 antibodies can prevent axon degeneration and motor neuron damage by blocking DR6. Previous studies demonstrated that PSAP localized to mitochondria and was required for DR6-induced… More >

  • Open Access

    ARTICLE

    Es-ATG7 is required for spermatogenesis of Eriocheir sinensis and modulates p53-dependent apoptosis in germ cells

    SHUANG-LI HAO, FEI-DA NI, BANG-HONG WEI, ZHEN-FANG LI, TONG YANG, WAN-XI YANG*
    BIOCELL, Vol.45, No.4, pp. 971-984, 2021, DOI:10.32604/biocell.2021.015178
    Abstract Spermatogenesis is a complicated and highly regulated male gamete differentiation process that begins with the proliferation and differentiation of spermatogonia to the release of the mature spermatozoa. The autophagy-related gene atg7 has been reported as closely related to spermatogenesis and communication of Sertoli cell-germ cells in mice, including acrosome biogenesis, sperm flagellum development, and ectoplasmic specialization assembly. However, the function of es-ATG7 and its molecular regulatory mechanism during spermatogenesis in Crustacea remain largely unknown. Here, we cloned and identified es-atg7 from the testes of the Chinese mitten crabs Eriocheir sinensis and found that the expression of es-atg7 was relatively high… More >

  • Open Access

    ARTICLE

    Fibroblast growth factor 9 promotes kidney cell proliferation via WNT signaling-mediated activation of ANXA4

    TING LI1,#, XINHUI SUN2,#, NANNAN LI1, HONGMIN GUO2,*
    BIOCELL, Vol.45, No.4, pp. 985-994, 2021, DOI:10.32604/biocell.2021.012371
    Abstract Fibroblast growth factors (FGFs) play pivotal roles in cell migration and proliferation. However, the identity of the FGF that plays a dominant role in kidney cell proliferation remains unclear. Therefore, in this study, we investigated the dominant FGF among all FGFs. To this end, RNA-sequencing, qRT-PCR, western blotting, and ChIP assays were performed. FGF9 showed the highest expression among all FGFs, and its overexpression significantly promoted proliferation in the mouse kidney cell line C57BL/6 and increased JNK and AKT phosphorylation levels. Further, RNA-seq analysis identified 365 upregulated and 276 downregulated genes in FGF9-overexpressed cells. These differentially expressed genes were classified… More >

  • Open Access

    ARTICLE

    Upregulation of microRNA-451a improves endothelial cell function in atherosclerosis by direct targeting of macrophage migration inhibitory factor

    HONGXIA HU*, PING GUO, QIAN ZHAO, HAORAN LI, HUALEI LIU, CAIHONG MA
    BIOCELL, Vol.45, No.4, pp. 995-1004, 2021, DOI:10.32604/biocell.2021.012851
    Abstract This study aims to detect the expression of selected circulating microRNAs (miRNA), including miRNA-451a, miRNA-486-5p and miR-10b-5p, and their prospective roles as biomarkers in patients with atherosclerosis. For this purpose, levels of miRNAs were detected by real-time quantitative polymerase chain reaction (RT-qPCR) in case (N = 30) and healthy control (N = 30) groups. Receiver operating characteristic (ROC) curve analysis was carried out to evaluate the diagnostic ability of miRNAs. The correlations of miR-451a with lipid parameters were evaluated using Pearson’s correlation coefficients. HUVEC tested by ox-LDL was used as a cellular model of atherosclerosis. Cell viability and apoptosis were… More >

  • Open Access

    ARTICLE

    Protective effects of Dioscin on TNF-α-induced collagen-induced arthritis rat fibroblast-like synoviocytes involves in regulating the LTB4/BLT pathway

    ZHIPING WEI1,2, YAJUN LIU1, MEIWEN YANG3, MENGDI LI1, KEXIN LI1, LUXI ZHENG1, HUIQIONG GUO1, FENFANG HONG4,*, SHULONG YANG1,*
    BIOCELL, Vol.45, No.4, pp. 1005-1012, 2021, DOI:10.32604/biocell.2021.014581
    Abstract Background and Objective: LTB4 has been shown to be involved in rheumatoid arthritis (RA) pathogenesis. The effect of Dioscin(Dio) on the LTB4 pathway of RA have not been reported yet. This study aimed at further exploring whether Dioscin’s effects on TNF-α induced collagen-induced arthritis (CIA) rat fibroblast-like synoviocytes (FLS) connected with the LTB4 and its receptor pathway. Materials & Methods: In this experiment, control group, TNF-α group, and different concentrations of Dioscin groups were established. Cell viability was evaluated using MTT assay. The levels of LTB4 in the samples of above groups were measured using ELISA. The mRNA expression levels… More >

  • Open Access

    ARTICLE

    Ultrastructural analysis shows persistence of adhesion and tight junction proteins in mature human hair

    LORENZO ALIBARDI1, BERND NOECKER2
    BIOCELL, Vol.45, No.4, pp. 1013-1022, 2021, DOI:10.32604/biocell.2021.013913
    Abstract The differentiation of cells composing mature human hairs produces layers with different corneous characteristics that would tend to flake away one from another, as in the corneous layer of the epidermis, without anchoring junctions. It is likely that cell junctions established in the forming cells of the hair bulb are not completely degraded like in the corneous layer of the epidermis but instead remain in the hair shaft to bind mature cuticle, cortex, and medulla cells into a compact hair shaft. During cell differentiation in hairs, cell junctions seem to disappear, and little is known about the fate of junctional… More >

  • Open Access

    ARTICLE

    Three-month effects of corneal cross-linking on corneal fibroblasts

    XINYAN CHEN1,2, HAIXIA ZHANG1,2, LIN LI1,2,*
    BIOCELL, Vol.45, No.4, pp. 1023-1032, 2021, DOI:10.32604/biocell.2021.014873
    (This article belongs to this Special Issue: Cellular Biomechanics in Health and Diseases)
    Abstract Corneal collagen crosslinking (CXL) has revolutionized the treatment of keratoconus in the past decade. In order to evaluate the 3-month effects of CXL on corneal fibroblasts, a longitudinal study at the tissue and cellular level was carried out with a total of 16 rabbits that underwent CXL, deepithelialization (DEP), or non-treatment (control) and kept for 1 to 3 months. The duration of corneal stromal remodeling after CXL was determined by examining the differentiation, apoptosis, and number changes of keratocytes in tissue sections from animals 1, 2, or 3 months post-treatment. Upon the finish of tissue remodeling, separate rabbits were used… More >

  • Open Access

    ARTICLE

    Long intergenic noncoding RNAs differentially expressed in Staphylococcus aureus-induced inflammation in bovine mammary epithelial cells

    JINGPENG ZHOU1,2,#, XIAOGUANG JI3,#, YUHAO WANG1,2, XIAOLONG WANG1,2, YONGJIANG MAO1,2,*, ZHANGPING YANG1,2,*
    BIOCELL, Vol.45, No.4, pp. 1033-1044, 2021, DOI:10.32604/biocell.2021.015586
    (This article belongs to this Special Issue: Decoding Gene (including circRNA, lincRNA miRNA and mRNA) Expression)
    Abstract Cow mastitis is the most common disease that affects the dairy farming industry and causes serious harm to dairy cows and humans, and Staphylococcus (S.) aureus is one of the main pathogens that cause mastitis in dairy cows. In this study, a mastitis model was established through the infection of bovine mammary epithelial cells (BMECs) with S. aureus (bacterial concentration of 1 × 109/mL), and these cells and a blank group (untreated) were analyzed by flow cytometry (10000 cells, 200 cells collected per second), hematoxylin and eosin (H&E) staining and immunohistochemistry. In addition, the lncRNAs (long non-coding RNAs) in the… More >

  • Open Access

    ARTICLE

    Angelica sinensis polysaccharides ameliorate 5-flourouracil-induced bone marrow stromal cell proliferation inhibition via regulating Wnt/β-catenin signaling

    HANXIANZHI XIAO, RONGJIA QI, ZILING WANG, MINGHE XIAO, YUE XIANG, YAPING WANG, LU WANG*
    BIOCELL, Vol.45, No.4, pp. 1045-1058, 2021, DOI:10.32604/biocell.2021.015039
    Abstract Chemotherapy may cause cellular oxidative stress to bone marrow. Oxidative damage of bone marrow hematopoietic microenvironment is closely related to chronic myelosuppression after chemotherapeutic treatment. Angelica sinensis polysaccharides (ASP) are major effective ingredients of traditional Chinese medicine Angelica with multi-target anti-oxidative stress features. In the current study, we investigated the protective roles and mechanisms of ASP on chemotherapy-induced bone marrow stromal cell (BMSC) damage. The human bone marrow stromal cell line HS-5 cells were divided into control group, 5-FU group, 5-FU + ASP group, and 5-FU + LiCl group to investigate the mechanism of ASP to alleviate 5-FU-induced BMSC proliferation… More >

  • Open Access

    ARTICLE

    Nicotine and menthol independently exert neuroprotective effects against cisplatin- or amyloid- toxicity by upregulating Bcl-xl via JNK activation in SH-SY5Y cells

    YIBIN RUAN1, ZHONGMING XIE2, QIONG LIU2, LIXIAO ZHANG2, XIKUI HAN2, XIAOYAN LIAO2, JIAN LIU1,*, FENGGUANG GAO2,*
    BIOCELL, Vol.45, No.4, pp. 1059-1067, 2021, DOI:10.32604/biocell.2021.015261
    Abstract Nicotine and menthol, agonists of nicotinic acetylcholine receptor (nAChR) and transient receptor potential melastatin type 8 (TRPM8), serve important roles in the prevention of cell death-involved neurodegenerative diseases. However, the potential synergistic effects of nicotine and menthol on anti-apoptotic ability are still uncertain. In the present study, the potential synergistic effects of nicotine and menthol on cisplatin or amyloid β1-42 induced cell model of the neurodegenerative diseases were explored by assessing cell viability, TNF-α expression, caspase-3 activation, and the collapse of mitochondrial membrane potential in human SH-SY5Y neuroblastoma cells. Statistical significance was tested using Student’s t-test or one-way ANOVA with… More >

  • Open Access

    REVIEW

    Development of high yield and tomato yellow leaf curl virus (TYLCV) resistance using conventional and molecular approaches: A review

    THARANGANI WELEGAMA1, MOHD Y. RAFII1,2,*, KHAIRULMAZMI AHMAD1,3, SHAIRUL I. RAMLEE1,2, YUSUFF OLADOSU1
    BIOCELL, Vol.45, No.4, pp. 1069-1079, 2021, DOI:10.32604/biocell.2021.014354
    Abstract Tomato (Solanum lycopersicum L.) belonging to the family Solanaceae is the second most consumed and cultivated vegetable globally. Since the ancient time of its domestication, thousands of cultivated tomato varieties have been developed targeting an array of aspects. Among which breeding for yield and yield-related traits are mostly focused. Cultivated tomato is extremely genetically poor and hence it is a victim for several biotic and abiotic stresses. Among the biotic stresses, the impact of viral diseases is critical all over tomato cultivating areas. Improvement of tomato still largely rely on conventional methods worldwide while molecular approaches, particularly Marker Assisted Selection… More >

  • Open Access

    ARTICLE

    Zinc oxide nanoparticles and epibrassinolide enhanced growth of tomato via modulating antioxidant activity and photosynthetic performance

    MOHAMMAD FAIZAN1, AHMAD FARAZ2, SHAMSUL HAYAT3, JAVAID A. BHAT4,*, FANGYUAN YU1,*
    BIOCELL, Vol.45, No.4, pp. 1081-1093, 2021, DOI:10.32604/biocell.2021.015363
    (This article belongs to this Special Issue: Molecular and Cellular Nanobiotechnology)
    Abstract Nanotechnology has greatly expanded the applications of nanoparticles (NPs) domain in the scientific field. In this context, the zinc oxide nanoparticles (ZnO-NPs) and 24-epibrassinolide (EBL) has been revealed to positively regulate plant metabolism and growth. In the present study, we investigated the role of ZnO-NPs and EBL in the regulation of plant growth, photosynthetic efficiency, enzymes activities and fruit yield in tomato. Foliar treatment of ZnO-NPs at three levels (10, 50 or 100 ppm) and EBL (10−8 M) were applied separately or in combination to the foliage of plant at 35–39 days after sowing (DAS); and the control plants were… More >

  • Open Access

    ARTICLE

    Development and characterization of Simple Sequence Repeat (SSR) markers from the genomic sequence of sweet potato [Ipomoea batatas L. (Lam)]

    HANNA AMOANIMAA-DEDE, JIACHENG ZHANG, CHUNTAO SU, HONGBO ZHU*
    BIOCELL, Vol.45, No.4, pp. 1095-1105, 2021, DOI:10.32604/biocell.2021.015053
    (This article belongs to this Special Issue: Advances in Plant Cell Biology Research)
    Abstract Sweet potato is a multifunctional root crop with many essential nutrients and bioactive compounds. Due to its genetic complexity and lack of genomic resources, efficient genetic studies and cultivar development lag far behind other major crops. Simple sequence repeats (SSRs) offer an effective molecular marker technology for molecular-based breeding and for locating important loci in crop plants, but only a few have previously been developed in sweet potato. To further explore new SSR markers and accelerate their use in sweet potato genetic studies, genome-wide characterization and development of SSR markers were performed using the recently published genome of sweet potato… More >

  • Open Access

    ARTICLE

    Genome-wide identification of WRKY gene family and expression analysis under abiotic stresses in Andrographis paniculata

    QICHAO WANG1, WUJING ZENG1, BASHARAT ALI2, XUEMIN ZHANG3, LING XU1,*, ZONGSUO LIANG1,*
    BIOCELL, Vol.45, No.4, pp. 1107-1119, 2021, DOI:10.32604/biocell.2021.015282
    Abstract Andrographis paniculata (A. paniculata) is a Chinese herbal medicine that clears away heat, reduces inflammation, protects the liver, and promotes choleretics. The WRKYs of A. paniculata are still not well characterized, although many WRKYs have been identified in various plant species. In the present study, 59 A. paniculata WRKY (ApWRKY) genes were identified and renamed on the basis of their respective chromosome distribution. These ApWRKYs were divided into three groups via phylogenetic analysis according to their WRKY domains and combined with WRKY of Arabidopsis. The 59 identified ApWRKY transcription factors were nonuniformity distributed on 23 chromosomes of A. paniculata. From… More >

  • Open Access

    ARTICLE

    Overexpression of rice F-box phloem protein gene OsPP12-A13 confers salinity tolerance in Arabidopsis

    CHUNKUN FAN, YONGPENG ZHANG, CHUNBAO YANG, YAWEI TANG, JI QU, BU JIE, DEJI QUZHEN, LIYUN GAO*
    BIOCELL, Vol.45, No.4, pp. 1121-1135, 2021, DOI:10.32604/biocell.2021.014336
    Abstract Salinity is a serious challenge for agriculture production by limiting the arable land. Rice is a major staple food crop but very sensitive to salt stress. In this study, we used Arabidopsis for the functional characterization of a rice F-box gene LOC_Os04g48270 (OsPP12-A13) under salinity stress. OsPP12-A13 is a nuclear-localized protein that is strongly upregulated under salinity stress in rice and showed the highest expression in the stem, followed by roots and leaves. Two types of transgenic lines for OsPP12-A13 were generated, including constitutive tissue over-expression using the CaMV35S promoter and phloem specific over-expression using the pSUC2 promoter. Both types… More >

  • Open Access

    ARTICLE

    Incidence, genomic diversity, and evolution of strawberry mottle virus in China

    LINGJIAO FAN, CHENGYONG HE, MENGMENG WU, DEHANG GAO, ZHENFEI DONG, SHENGFAN HOU, ZEKUN FENG, HONGQING WANG*
    BIOCELL, Vol.45, No.4, pp. 1137-1151, 2021, DOI:10.32604/biocell.2021.015396
    Abstract Strawberry mottle virus (SMoV) is one of the most common viruses infecting strawberries, causing losses to fruit yield and quality. In this study, 165 strawberry leaf samples were collected from six provinces of China, 46 of which tested positive for SMoV. The complete genome sequences of 11 SMoV isolates were obtained from Liaoning (DGHY3, DGHY16-2, DGHY17, DGHY20-2, DGHY21, DGHY26-2), Shandong (SDHY1, SDHY5, SDHY31-2, SDHY33-2), and Beijing (BJMX7). The RNA1 and RNA2 nucleotide identities between the 11 Chinese isolates were 95.4–99.3% and 96.3–99.6%, respectively, and they shared 78.4–96.6% and 84.8–93.5% identities with the available SMoV isolates in GenBank. Recombination analysis revealed… More >

  • Open Access

    ARTICLE

    Genome-wide identification and expression analysis of Aux/IAA gene family in strawberry (Fragaria vesca)

    LIYAN SU1,2,#, HONG YU3,#, JIUDONG ZHANG1, XIAOHONG ZHAO1, XUAN LIU1, QIANG LI1,4,*
    BIOCELL, Vol.45, No.4, pp. 1153-1166, 2021, DOI:10.32604/biocell.2021.014499
    Abstract Auxin signaling and its components (Auxin/Indole-3-Acetic Acid (Aux/IAA)) are critical for plant growth and development. Here, we performed a genome-wide annotation and identified twenty-one Aux/IAA genes in strawberry (Fragaria vesca). Most FveIAAs were located on chromosomes 1, 2, 4, 5, and 6, while no FveIAAs were found in chromosomes 3 and 7. Phylogenetic analysis divided these genes into nine subfamilies. Most FveIAAs contained the DNA-binding and Aux/IAA domains, as well as motifs I–IV. There were 2–6 exons in the FveIAA genes based on the gene structure analysis. Also, we found that four pairs of FveIAA genes underwent segment duplications. Moreover,… More >

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