Home / Journals / BIOCELL / Vol.33, No.2, 2009
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  • Open Access

    ARTICLE

    Immunohistochemistry of GluR1 subunits of AMPA receptors of rat cerebellar nerve cells

    ORLANDO J. CASTEJÓN1*, MICHAEL E. DAILEY2
    BIOCELL, Vol.33, No.2, pp. 71-80, 2009, DOI:10.32604/biocell.2009.33.071
    Abstract The localization of GluR1 subunits of ionotropic glutamate receptors in the glial cells and inhibitory neurons of cerebellar cortex and their association with the climbing and parallel fibers, and basket cell axons were studied. Samples of P14 and P21 rat cerebellar cortex were exposed to a specific antibody against GluR1 subunit(s) of AMPA receptors and were examined with confocal laser scanning microscopy. GluR1 strong immunoreactivity was confined to Purkinje cell and the molecular layer. Weak GluR1 immunoreactivity was observed surrounding some Golgi cells in the granule cell layer. Intense GluR1 immunoreactivity was localized around Purkinje, basket, and stellate cells. Purkinje… More >

  • Open Access

    ARTICLE

    Novel neurotrophic factor secreted by amniotic epithelial cells

    SANKAR VENKATACHALAM, TAMILSELVI PALANIAPPAN, PREM KUMAR JAYAPAL, SRIDHARAN NEELAMEGAN, SRIDHAR SKYLAB RAJAN, VIJAYA PRAKASH KRISHNAN MUTHIAH
    BIOCELL, Vol.33, No.2, pp. 81-89, 2009, DOI:10.32604/biocell.2009.33.081
    Abstract By virtue of expressions of glial and neural surface markers and capability of neurotransmitter metabolism, amniotic epithelial cells are considered as candidate cell type for transplantation strategies to treat neurological disorders. Previously, we have reported neurotrophism exhibited by human amniotic epithelial cells when transplanted after spinal cord injury in bonnet monkeys. Amniotic epithelial cells were believed to secrete an “Epidermal Growth Factor (EGF) - like” factor and exact identification was not made. At this juncture, through the present study it was found that, chicken neural retinal cells when grown alone failed to survive and contrarily when either co-cultured with chicken… More >

  • Open Access

    ARTICLE

    Impact of pituitary FSH purification on in vitro early folliculogenesis in goats

    D.M. MAGALHÃES1*, V.R. ARAÚJO1, I.B. LIMA-VERDE1, M.H.T. MATOS1, R.C. SILVA2, C.M. LUCCI2, S.N. BÁO2, C.C. CAMPELLO1, J.R. FIGUEIREDO1
    BIOCELL, Vol.33, No.2, pp. 91-97, 2009, DOI:10.32604/biocell.2009.33.091
    Abstract Porcine pituitary follicle stimulating hormone (pFSH) is known to regulate the production of growth factors that have an essential role in early foliculogenesis. However, the effects of different preparations of pFSH on the survival and development of caprine follicles are not yet known. The aim of this study was to evaluate the effects of different pFSH (Stimufol® and Folltropin®) on the in vitro survival and growth of caprine preantral follicles. Pieces of caprine ovarian tissues were cultured for either one or seven days in a supplemented Minimum Essential Medium, alone or containing either Stimufol® (50 ng/mL) or Folltropin® (10, 50,… More >

  • Open Access

    ARTICLE

    Storage lipids and proteins of Euterpe edulis seeds

    VÍCTOR PANZA1,2, DARIO PIGHIN3, VERÓNICA LÁINEZ2, RICARDO J. POLLERO4, SARA MALDONADO1,2,*
    BIOCELL, Vol.33, No.2, pp. 99-106, 2009, DOI:10.32604/biocell.2009.33.099
    Abstract Comparative studies on fatty acid and protein composition of the endosperm and embryo of palmito (Euterpe edulis Martius) were conducted using gas-liquid chromatography and sodium dodecyl sulfate–polyacrylamide gel electrophoresis. On a dry weight basis, the embryo contained extremely lower amounts of lipids and proteins than did the endosperm, which was associated with the scarce lipid and protein bodies previously reported in axis and cotyledon. The fatty acid composition also exhibited differences between both tissues: (I) the fatty acid diversity was greater in embryo than in endosperm; (II) embryo and endosperm contained predominantly linoleic, palmitic, oleic and stearic acids even though… More >

  • Open Access

    ARTICLE

    Simvastatin acts as an inhibitor of interferon gamma-induced cycloxygenase-2 expression in human THP-1 cells, but not in murine RAW264.7 cells

    CHANG SEOK LEE1, YONG JAE SHIN1, CHEOLHEE WON1, YUN-SONG LEE2, CHUNG-GYU PARK3, SANG-KYU YE1*, MYUNG-HEE CHUNG1
    BIOCELL, Vol.33, No.2, pp. 107-114, 2009, DOI:10.32604/biocell.2009.33.107
    Abstract Cyclooxygenase-2 (COX-2) is a key inflammatory response molecule, and associated with many immune functions of monocytes/macrophages. Particularly, interferon gamma (IFNγ)-induced COX-2 expression appears in inflammatory conditions such as viral infection and autoimmune diseases. Recently, statins have been reported to show variable effects on COX-2 expression, and on their cell and species type dependences. Based on the above description, we compared the effect of simvastatin on IFNγ-induced COX2 expression in human monocytes versus murine macrophages. In a result, we found that simvastatin suppresses IFNγ-induced COX-2 expression in human THP-1 monocytes, but rather, potentiates IFNγ-induced COX-2 expression in murine RAW264.7 macrophages. However,… More >

  • Open Access

    ARTICLE

    A six-rhabdomere, open rhabdom arrangement in the eye of the chrysanthemum beetle Phytoecia rufiventris: some ecophysiological predictions based on eye anatomy

    VICTOR BENNO MEYER-ROCHOW1,2,*, MONALISA MISHRA1
    BIOCELL, Vol.33, No.2, pp. 115-120, 2009, DOI:10.32604/biocell.2009.33.115
    Abstract We are describing a rhabdom organization of the eye of the chrysanthemum beetle Phytoecia rufiventris that to date has not been described from any other insect. In cerambycid beetles free rhabdomeres, forming a circular, open rhabdom, surround a central rhabdom made up of the rhabdomeres of one or two cells. In Phytoecia rufiventris the central rhabdomeres are missing throughout the eye and the microvilli of the outer 6 rhabdomeres are regularly oriented in three directions. Following the classification of rhabdom types suggested by Wachmann (1979), we suggest to name the rhabdom arrangement seen in the retina of Phytoecia rufiventris “Grundmuster… More >

  • Open Access

    ARTICLE

    Apoptosis as pathogenic mechanism of infection with vesicular stomatitis virus. Evidence in primary bovine fibroblast cultures

    A. LÓPEZ-HERRERA1*, J. RUIZ-SÁENZ2, Y.P. GÓEZ3, W. ZAPATA3, P.A. VELILLA3, A.E. ARANGO3, S. URCUQUI-INCHIMA3
    BIOCELL, Vol.33, No.2, pp. 121-132, 2009, DOI:10.32604/biocell.2009.33.121
    Abstract To determine whether fibroblasts from Blanco Orejinegro cattle, exhibit any level of resistance to infection against vesicular stomatitis virus (VSV) serotypes Indiana (VSV-I) or New Jersey (VSVNJ), 30 fibroblast cultures were phenotyped to evaluate their resistance/susceptibility. Thirty three % of Blanco Orejinegro fibroblast cultures were classified as very resistant, 50% as resistant, and 17% as susceptible to VSV-I infection, whereas 20% were classified as very resistant, 50% as resistant and 30% as susceptible to VSV-NJ infection. Therefore, there appears to be a large variation in phenotypic polymorphism among the fibroblasts to infection by VSV. To elucidate the mechanisms responsible for… More >

  • Open Access

    ARTICLE

    Brief note: Differences in intracellular localization of corn stunt spiroplasmas in magnesium treated maize.

    CLAUDIA NOME1*, PAULO CÉSAR MAGALHÃES2, ELIZABETH OLIVEIRA2, SERGIO NOME, IRMA GRACIELA LAGUNA1
    BIOCELL, Vol.33, No.2, pp. 133-136, 2009, DOI:10.32604/biocell.2009.33.133
    Abstract Maize plants infected with Spiroplasma kunkelii show symptoms similar to that of plants in a magnesium-deficient soil, and it has been shown that the spiroplasma alters the plants’ magnesium absorption. In the current study we compared changes associated to either spiroplasma infection, two soil magnesium levels and their combinations. Plant symptoms were recorded and correlated with transmission electron microscopy observations. Plants grown on a high magnesium treatment showed no macroscopical alterations nor organelle ultrastructural alterations, while plants on a low magnesium treatment showed macroscopical vein yellowing and, ultrastructurally, they had most chloroplasts and mitochondrial membranes altered. Infected plants on a… More >

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