Home / Journals / BIOCELL / Vol.47, No.6, 2023
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  • Open AccessOpen Access

    REVIEW

    Protein-mediated interactions in the dynamic regulation of acute inflammation

    RYAN STARK*
    BIOCELL, Vol.47, No.6, pp. 1191-1198, 2023, DOI:10.32604/biocell.2023.027838
    (This article belongs to this Special Issue: Biochemical and Epigenetics Changes in Health and Disease)
    Abstract Protein-mediated interactions are the fundamental mechanism through which cells regulate health and disease. These interactions require physical contact between proteins and their respective targets of interest. These targets include not only other proteins but also nucleic acids and other important molecules as well. These proteins are often involved in multibody complexes that work dynamically to regulate cellular health and function. Various techniques have been adapted to study these important interactions, such as affinity-based assays, mass spectrometry, and fluorescent detection. The application of these techniques has led to a greater understanding of how protein interactions are responsible for both the instigation… More >

  • Open AccessOpen Access

    REVIEW

    The progress of combination therapy with immune checkpoint inhibitors in breast cancer

    KAIMIN FAN, JUNWEI WENG*
    BIOCELL, Vol.47, No.6, pp. 1199-1211, 2023, DOI:10.32604/biocell.2023.028516
    (This article belongs to this Special Issue: Immunotherapy for Breast Cancer)
    Abstract Immunotherapy targets the dysfunctional immune system to induce cancer cell killing by CD8-positive T cells. Immune checkpoint inhibitors (ICIs), specifically anti-PD-1 antibodies, anti-PD-L1 antibodies, and anti-CTLA4 antibodies, have revolutionized the management of many malignancies due to their significant role in generating a durable clinical response. However, clinical data suggest that response rates to ICI monotherapy are low due to the immunologically silent characteristics of breast cancer (BC). Chemotherapy, surgery, radiotherapy, and targeted therapy were recently reported to alter the tumor microenvironment and enhance the ICI response. Some clinical studies supported that ICIs, in combination with other treatment strategies, show superior… More >

  • Open AccessOpen Access

    REVIEW

    Recent progress in microbial cell surface display systems and their application on biosensors

    HAIYING CHEN, YUQING WU, BAOJIAN HUANG, LEI HAN*
    BIOCELL, Vol.47, No.6, pp. 1213-1223, 2023, DOI:10.32604/biocell.2023.028035
    Abstract Microbial cell surface display technology is a recombinant technology to express target proteins on the cell membrane, which can be used to redesign the cell surface with functional proteins and peptides. Bacterial and yeast surface display systems are the most common cell surface display systems of prokaryotic and eukaryotic proteins, that are widely applied as the core elements in the field of biosensors due to their advantages, including enhanced stability, high yield, good safety, expression of larger and more complex proteins. To further promote the performance of biosensors, the biomineralized microbial surface display technology was proposed. This review summarized the… More >

  • Open AccessOpen Access

    REVIEW

    A broad overview of genotype imputation: Standard guidelines, approaches, and future investigations in genomic association studies

    MIRKO TRECCANI*, ELENA LOCATELLI, CRISTINA PATUZZO, GIOVANNI MALERBA*
    BIOCELL, Vol.47, No.6, pp. 1225-1241, 2023, DOI:10.32604/biocell.2023.027884
    (This article belongs to this Special Issue: Bioinformatics Study of Diseases)
    Abstract The advent of genomic big data and the statistical need for reaching significant results have led genome-wide association studies to be ravenous of a huge number of genetic markers scattered along the whole genome. Since its very beginning, the so-called genotype imputation served this purpose; this statistical and inferential procedure based on a known reference panel opened the theoretical possibility to extend association analyses to a greater number of polymorphic sites which have not been previously assayed by the used technology. In this review, we present a broad overview of the genotype imputation process, showing the most known methods and… More >

  • Open AccessOpen Access

    ARTICLE

    Vitamin D attenuates TGF-β1-induced lung fibroblast proliferation and migration through repression of RasGRP3

    GUANQIONG HU1, RISHENG HUANG2, LUELUE LU1, QINSHI PAN3, XIAOXIN CHEN1,*
    BIOCELL, Vol.47, No.6, pp. 1243-1251, 2023, DOI:10.32604/biocell.2023.027763
    (This article belongs to this Special Issue: Biochemical and Epigenetics Changes in Health and Disease)
    Abstract Background: Transforming growth factor-β1 (TGF-β1) is a pleiotropic cytokine that plays a central role in the pathogenesis of idiopathic pulmonary fibrosis (IPF). While previous studies have revealed a cross-talk between vitamin D and TGF-β1 signaling, it is still unclear how they interact with each other to regulate the progression of IPF. Methods: In this work, we searched for a novel mediator of TGF-β1 activity in lung fibroblasts and examined its regulation by vitamin D. In addition, we investigated the mechanism underlying the interaction between vitamin D and TGF-β1 signaling in lung fibroblast activation. Bioinformatic analysis was performed to identify TGF-β1… More >

  • Open AccessOpen Access

    ARTICLE

    Genetic algorithm-optimized backpropagation neural network establishes a diagnostic prediction model for diabetic nephropathy: Combined machine learning and experimental validation in mice

    WEI LIANG1,2,*, ZONGWEI ZHANG1,2, KEJU YANG1,2,3, HONGTU HU1,2, QIANG LUO1,2, ANKANG YANG1,2, LI CHANG4, YUANYUAN ZENG4
    BIOCELL, Vol.47, No.6, pp. 1253-1263, 2023, DOI:10.32604/biocell.2023.027373
    (This article belongs to this Special Issue: Bioinformatics Study of Diseases)
    Abstract Background: Diabetic nephropathy (DN) is the most common complication of type 2 diabetes mellitus and the main cause of end-stage renal disease worldwide. Diagnostic biomarkers may allow early diagnosis and treatment of DN to reduce the prevalence and delay the development of DN. Kidney biopsy is the gold standard for diagnosing DN; however, its invasive character is its primary limitation. The machine learning approach provides a non-invasive and specific criterion for diagnosing DN, although traditional machine learning algorithms need to be improved to enhance diagnostic performance. Methods: We applied high-throughput RNA sequencing to obtain the genes related to DN tubular… More >

  • Open AccessOpen Access

    ARTICLE

    Proteomics analysis provides novel biomarkers and therapeutic target candidates in the treatment of the Huang-Pu-Tong-Qiao formula in an AD rat model

    QIAN CHEN1,#, XIN LEI1,#, GUANHUA HU1,#, YAN WANG2, ZHENGQING FANG1, GUOQUAN WANG1, HANG SONG1, SHU YE1,*, BIAO CAI1,*
    BIOCELL, Vol.47, No.6, pp. 1265-1277, 2023, DOI:10.32604/biocell.2023.028811
    (This article belongs to this Special Issue: Bioinformatics Study of Diseases)
    Abstract Background: Huang-Pu-Tong-Qiao formula (HPTQ), a traditional Chinese herbal formula, has a variety of pharmacological effects. It has been used to treat Alzheimer’s disease (AD) for decades. This study aimed to screen differentially expressed proteins in the hippocampus of AD model rats treated with HPTQ. Proteomic studies of the effects of HPTQ on AD are key to understanding the therapeutic mechanisms of HPTQ and identifying potential therapeutic targets. Methods: We hence used the isobaric tags for relative and absolute quantification (ITRAQ) approach to investigate the differentially expressed proteins in the hippocampus of AD model rats before and after HPTQ administration and… More >

  • Open AccessOpen Access

    ARTICLE

    Prognosis and immunological role of HLA-DMA in lung adenocarcinoma

    QIN YU1,2,#, CHEN CHEN4,5,#, HAIYAN ZHANG6,#, JIN CHEN2, JUNKANG SHEN1,3,*, JUN YAN5,*
    BIOCELL, Vol.47, No.6, pp. 1279-1292, 2023, DOI:10.32604/biocell.2023.027553
    (This article belongs to this Special Issue: Bioinformatics Study of Diseases)
    Abstract Background: HLA-DMA presents pathogen-derived antigens to CD4+ and CD8+ T cells, respectively, and plays a significant part in initiating the immune response. So far, the impact of HLA expression on the prognosis of BC cells is controversial, because few studies have shown that the expressions of some HLA genes are related to the improvement of the survival rate. Up till now, however, the relationship between HLA-DMA and LUAD has not yet been assessed. Methods: We analyzed the TCGA database and assessed the prognostic value of HLA-DMA in LUAD. We conducted the Kruskal–Wallis and Wilcoxon signed-rank test and utilized logistic regression… More >

  • Open AccessOpen Access

    ARTICLE

    Bioinformatic analysis of lncRNA-associated competing endogenous RNA regulatory networks in synovial tissue of temporomandibular joint osteoarthritis

    CHUYAO WANG1,2,#, CHUAN LU2,#, LUXIANG ZOU2,*, DONGMEI HE2,*
    BIOCELL, Vol.47, No.6, pp. 1293-1306, 2023, DOI:10.32604/biocell.2023.028199
    (This article belongs to this Special Issue: Bioinformatics Study of Diseases)
    Abstract Background: Temporomandibular joint osteoarthritis (TMJOA) is an end-stage disease that seriously affects the patients’ quality of life. Molecular insights in advancing our understanding of TMJOA are the need of the hour. Methods: We performed RNA high-throughput sequencing and bioinformatics analysis of differentially expressed (DE) long non-coding RNA (lncRNAs), microRNAs (miRNAs), and messenger RNA (mRNAs) in human synovial TMJOA tissues. Firstly, synovium samples of TMJOA patients and non-TMJOA controls were collected for highthroughput sequencing of lncRNAs, miRNAs, and mRNAs. We then performed biological function analysis of the top 100 mRNAs with more than 2-fold differential expression, and their upstream regulated miRNAs… More >

  • Open AccessOpen Access

    ARTICLE

    Scutellarin alleviates complete freund’s adjuvant-induced rheumatoid arthritis in mice by regulating the Keap1/Nrf2/HO-1 pathway

    JIAN LI1, QINGQING WANG2, XIAOYING ZHANG1,*
    BIOCELL, Vol.47, No.6, pp. 1307-1316, 2023, DOI:10.32604/biocell.2023.028714
    (This article belongs to this Special Issue: Natural Products for Chronic Inflammatory Diseases: Pharmacology and Toxicology)
    Abstract Scutellarin (SCU) is a herbal flavonoid glucuronide with multiple pharmacological activities, including anti-oxidant, anti-inflammation, vascular relaxation, anti-platelet, and myocardial protection. However, the effect of SCU on complete Freund’s adjuvant (CFA)-induced rheumatoid arthritis (RA) had not been studied. In this study, we investigated the beneficial effects of SCU in the CFA-induced RA mice model and the anti-arthritic activity was evaluated by paw edema. Enzyme-linked immunosorbent assay (ELISA) was carried out to evaluate the plasma levels of immunoglobulin (Ig)G, IgE, tumor necrosis factor (TNF)-α, interleukin (IL)-1β, IL-6, receptor activator of nuclear factor-κB ligand (RANKL), and osteoprotegerin (OPG). Histological slides were prepared from… More >

  • Open AccessOpen Access

    ARTICLE

    Exploring the mechanisms of magnolol in the treatment of periodontitis by integrating network pharmacology and molecular docking

    DER-JEU CHEN, CHENG-HUNG LAI*
    BIOCELL, Vol.47, No.6, pp. 1317-1327, 2023, DOI:10.32604/biocell.2023.028883
    (This article belongs to this Special Issue: Natural Products for Chronic Inflammatory Diseases: Pharmacology and Toxicology)
    Abstract Background: Magnolol, a bioactive extract of the Chinese herb Magnolia officinalis has a protective effect against periodontitis. This study is aimed to explore the mechanisms involved in the functioning of magnolol against periodontitis and provide a basis for further research. Methods: Network pharmacology analysis was performed based on the identification of related targets from public databases. The Protein-protein interaction (PPI) network was constructed to visualize the significance between the targets of magnolol and periodontitis. Subsequently, Gene ontology (GO) and Kyoto Encyclopedia of Genes and Genomes (KEGG) analysis were performed to predict the functions and the signal regulatory pathways involved in… More >

  • Open AccessOpen Access

    ARTICLE

    Therapeutic targets and signal transduction mechanisms of medicinal plant formula Gancao Xiexin decoction against ulcerative colitis: A network pharmacological study

    CHENHAO SHI1, MAOHONG HUA2, GUANHUA XU3,*
    BIOCELL, Vol.47, No.6, pp. 1329-1344, 2023, DOI:10.32604/biocell.2023.028381
    (This article belongs to this Special Issue: Natural Products for Chronic Inflammatory Diseases: Pharmacology and Toxicology)
    Abstract Background: Ulcerative colitis (UC) is a chronic disease that often presents with abdominal pain, diarrhea, hematochezia, and significant morbidity. Gancao Xiexin decoction (GXD), a traditional Chinese medicine, has been applied for the clinical treatment of UC, while its action mechanisms are unclear. Methods: The active ingredients and their targets of GXD, and UC-related targets, were derived from public databases. Protein-protein interaction, Gene Ontology (GO), and the Kyoto Encyclopedia of Genes and Genomes (KEGG) were used to analyze the important active compounds, key targets, and signaling pathways. Then, molecular docking and animal experiments were performed to verify the findings. A total… More >

  • Open AccessOpen Access

    ARTICLE

    TianmaGouteng yin attenuates ischemic stroke-induced brain injury by inhibiting the AGE/RAGE pathway

    LUOJUN ZHENG, LUAN WENG, DIWEN SHOU*
    BIOCELL, Vol.47, No.6, pp. 1345-1352, 2023, DOI:10.32604/biocell.2023.028866
    (This article belongs to this Special Issue: Natural Products for Chronic Inflammatory Diseases: Pharmacology and Toxicology)
    Abstract Background: Ischemic stroke is characterized by permanent or transient obstruction of blood flow, leading to a growing risk factor and health burden. Tianmagouteng yin (TMG) is commonly used in Chinese medicine to treat cerebral ischemia. The aim of this study was to investigate the neuroprotective effects of TMG against ischemic stroke. Methods: Either permanent middle cerebral artery occlusion (pMCAO) or sham operation was performed on anesthetized Wistar male rats (n = 36). Results: Results demonstrated that TMG administration reduced the infarction volume and mitigated the neurobehavioral deficits. Hematoxylin and eosin (HE) staining and Prussian blue staining revealed that TMG attenuated… More >

  • Open AccessOpen Access

    ARTICLE

    The correlation of miRNA expression and tumor mutational burden in uterine corpus endometrial carcinoma

    YANYA CHEN1,#, HONGYUAN WU2,#, RUISI ZHOU5,#, HELING DONG4, XUEFANG ZHANG2, XUEWEI WU1, WENSHAN CHEN1, YANTING YOU5,*, YIFEN WU3,*
    BIOCELL, Vol.47, No.6, pp. 1353-1364, 2023, DOI:10.32604/biocell.2023.027346
    Abstract Background: The relationship between microRNA (miRNA) expression patterns and tumor mutation burden (TMB) in uterine corpus endometrial carcinoma (UCEC) was investigated in this study. Methods: The UCEC dataset from The Cancer Genome Atlas (TCGA) database was used to identify the miRNAs that differ in expression between high TMB and low TMB sample sets. The total sample sets were divided into a training set and a test set. TMB levels were predicted using miRNA-based signature classifiers developed by Lasso Cox regression. Test sets were used to validate the classifier. This study investigated the relationship between a miRNA-based signature classifier and three… More >

  • Open AccessOpen Access

    ARTICLE

    Clinical implication of naive and memory T cells in locally advanced cervical cancer: A proxy for tumor biology and short-term response prediction

    YUTING WANG1,2,3, PEIWEN FAN1,2,3, YANING FENG1,2,3, XUAN YAO4, YANCHUN PENG4, RUOZHENG WANG1,2,3,*
    BIOCELL, Vol.47, No.6, pp. 1365-1375, 2023, DOI:10.32604/biocell.2023.027201
    Abstract Background: This study was designed to investigate the feasibility of tumor-infiltrating immune cells with different phenotypic characteristics for predicting short-term clinical responses in patients with locally advanced cervical cancer (LACC). Methods: Thirty-four patients who received concurrent chemoradiotherapy and twenty-one patients who merely underwent radiotherapy were enrolled in this study. We retrospectively analyzed the T cell markers (i.e., CD3, CD4, CD8), memory markers (i.e., CD45, CCR7), and differentiation markers (i.e., CD27) in the peripheral blood and tumor tissues of patients with LACC before treatment based on flow cytometry. We also analyzed the relationship of T cell subsets between peripheral blood and… More >

  • Open AccessOpen Access

    ARTICLE

    Light-controlled phosphorylation in the TrkA-Y785 site by photosensitive UAAs activates the MAPK/ERK signaling pathway

    SHU ZHAO1,*, SHIXIN YE2
    BIOCELL, Vol.47, No.6, pp. 1377-1388, 2023, DOI:10.32604/biocell.2023.023874
    Abstract Background: This paper aims to establish a light-controlled phosphorylation detection method at the Y785 site of tropomyosin receptor kinase A (TrkA) receptor in mammalian cells by using genetic code expansion technology and detecting the effects of optical activation of this site on the downstream MAPK/ERK pathway. The study is based on the current situation that the regulatory mechanism of TrkA phosphorylation has not been fully elucidated. Methods: Two photosensitive unnatural amino acids, p-azido-L-phenylalanine (AzF) and photo-caged tyrosine (ONB) were introduced into the TrkA-Y785 site by genetic code expansion technology and site-directed mutagenesis. Western blotting and laser confocal imaging were conducted… More >

  • Open AccessOpen Access

    ARTICLE

    Capsaicin exerts anti-benign prostatic hyperplasia effects via inhibiting androgen receptor signaling pathway

    ZICHEN SHAO1,2,#, CHUNG-YI CHEN3,#, XUZHOU CHEN1, HANWU CHEN1, MENGQIAO SU1,2, HUI SUN1,2, YIDAN LI1,2, BINGHUA TU1, ZITONG WANG1, CHI-MING LIU1,*
    BIOCELL, Vol.47, No.6, pp. 1389-1396, 2023, DOI:10.32604/biocell.2023.028222
    Abstract Background: Benign prostatic hyperplasia (BPH) is a common condition in middle-aged and elderly men. Enlargement of the prostate causes lower urinary tract symptoms. Capsaicin is a phytochemical extracted from chili peppers and exerts many pharmacological actions, such as anti-tumor and anti-inflammatory effects. Methods: Our study investigated the effect of capsaicin in vitro and in a mouse model in vivo. A prostatic stromal myofibroblast cell line (WPMY-1) was co-incubated with testosterone (1 µM) and different concentrations of capsaicin (10–100 µM) for 24 and 48 h. Capsaicin (10–100 µM) significantly inhibited testosterone-treated WPMY-1 cell growth at 48 h by MTT assay. The… More >

  • Open AccessOpen Access

    ARTICLE

    Inhibition of H2O2-induced TM3 cell apoptosis by oxidative stress by lentinan functionalized selenium nanoparticles through JAK2/STAT-3 and P53 pathways

    MIAOMIAO LI1,#, ZILIN ZHENG1,#, JUNYI KE1, JIEYI LUO1, FAN JIANG1, YANXIA QU1, BING ZHU2, YINGHUA LI2,*, LIANDONG ZUO1,*
    BIOCELL, Vol.47, No.6, pp. 1397-1405, 2023, DOI:10.32604/biocell.2023.027971
    Abstract Background: Nano-selenium has been widely used in antiviral and anticancer therapy, and has the advantages of good targeting and low toxicity. For the first time, we combined male reproduction with nano-selenium to investigate its antioxidant effect. This study investigated the protective effect of lentinan functionalized selenium nanoparticles on oxidative stress injury of the hydrogen peroxide (H2O2)-induced Leydig cell line, TM3. Methods: The suitable concentration of nano-selenium treatment to promote cell proliferation was also discussed. The concentration of 4 μM could significantly promote the growth of TM3 cells. Oxidative stress damage was caused using an 800 μM concentration of hydrogen peroxide.… More >

  • Open AccessOpen Access

    ARTICLE

    LncRNA ZFAS1 regulates cardiomyocyte differentiation of human embryonic stem cells

    YANG CAO1,#, YINING LIU1,#, YANG YU1, XIAOFEI GUO1, XIUXIU WANG1, WENYA MA1, HANJING LI2, ZHONGYU REN2, XINLU GAO2, SIJIA LI2, HAOYU JI2, HONGYANG CHEN2, HONG YAN2, YANAN TIAN2, XIN WANG2, BENZHI CAI1,2,*
    BIOCELL, Vol.47, No.6, pp. 1407-1416, 2023, DOI:10.32604/biocell.2023.029080
    Abstract Background: Cardiomyocytes derived from human embryonic stem cells (hESCs) are regulated by complex and stringent gene networks during differentiation. Long non-coding RNAs (lncRNAs) exert critical epigenetic regulatory functions in multiple differentiation processes. However, the involvement of lncRNAs in the differentiation of hESCs into cardiomyocytes has not yet been fully elucidated. Here, we identified the key roles of ZFAS1 (lncRNA zinc finger antisense 1) in the differentiation of cardiomyocytes from hESCs. Methods: A model of cardiomyocyte differentiation from stem cells was established using the monolayer differentiation method, and the number of beating hESCs-derived cardiomyocytes was calculated. Gene expression was analyzed by… More >

  • Open AccessOpen Access

    ARTICLE

    Effects of Tsukamurella tyrosinosolvens P9 on growth, physiology and antioxdant enzyme of peanut under drought stress and after re-watering

    CHANGMEI LONG, TINGTING YANG, YUJIE HAN, LIZHEN HAN*
    BIOCELL, Vol.47, No.6, pp. 1417-1430, 2023, DOI:10.32604/biocell.2023.027485
    (This article belongs to this Special Issue: Physiology and Molecular Biology of Plant Stress Tolerance)
    Abstract Background: The plant-growth-promoting rhizobacterium Tsukamurella tyrosinosolvens is a rare strain of actinomycete, in order to recognize and expand the ecological functions of rare actinomycetes. Methods: In this experiment, we studied the effect of Tsukamurella tyrosinosolvens P9 on the drought resistance of peanut by inoculating peanut seedlings in pots and measuring the growth and physiological indicators of peanut under drought stress and re-watering conditions. Results: The results showed that during drought stress, the relative water content of the soil and leaves, chlorophyll content, and stomatal length, width, and aperture were significantly decreased while the levels of malondialdehyde (MDA), H2O2 and stomatal… More >

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