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Light-controlled phosphorylation in the TrkA-Y785 site by photosensitive UAAs activates the MAPK/ERK signaling pathway

SHU ZHAO1,*, SHIXIN YE2

1 School of Life Sciences, Nantong University, Nantong, 226019, China
2 Institute National de la Sante et de la Recherche Med icale (INSERM) U1195, Bicetre Hospital, Paris-Saclay University, Le Kremlin-Bicêtre, 94276, France

* Corresponding Author: Shu Zhao, email

BIOCELL 2023, 47(6), 1377-1388. https://doi.org/10.32604/biocell.2023.023874

Abstract

Background: This paper aims to establish a light-controlled phosphorylation detection method at the Y785 site of tropomyosin receptor kinase A (TrkA) receptor in mammalian cells by using genetic code expansion technology and detecting the effects of optical activation of this site on the downstream MAPK/ERK pathway. The study is based on the current situation that the regulatory mechanism of TrkA phosphorylation has not been fully elucidated. Methods: Two photosensitive unnatural amino acids, p-azido-L-phenylalanine (AzF) and photo-caged tyrosine (ONB) were introduced into the TrkA-Y785 site by genetic code expansion technology and site-directed mutagenesis. Western blotting and laser confocal imaging were conducted to analyze the effects of this site on activating the MAPK/ERK pathway and nerve cell differentiation before and after photostimulation. Results: Our results supplemented the light-controlled results of the TrkA-Y785 site based on our previous research and verified that Y785 also makes important contributions in regulating the MAPK/ERK pathway. Conclusion: This study demonstrated the significant contributions of the TrkAY785 site in regulating the ERK pathway by precisely controlling the phosphorylation state of a single tyrosine site.

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APA Style
ZHAO, S., YE, S. (2023). Light-controlled phosphorylation in the trka-y785 site by photosensitive uaas activates the MAPK/ERK signaling pathway. BIOCELL, 47(6), 1377-1388. https://doi.org/10.32604/biocell.2023.023874
Vancouver Style
ZHAO S, YE S. Light-controlled phosphorylation in the trka-y785 site by photosensitive uaas activates the MAPK/ERK signaling pathway. BIOCELL . 2023;47(6):1377-1388 https://doi.org/10.32604/biocell.2023.023874
IEEE Style
S. ZHAO and S. YE, "Light-controlled phosphorylation in the TrkA-Y785 site by photosensitive UAAs activates the MAPK/ERK signaling pathway," BIOCELL , vol. 47, no. 6, pp. 1377-1388. 2023. https://doi.org/10.32604/biocell.2023.023874



cc This work is licensed under a Creative Commons Attribution 4.0 International License , which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
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