Open Access
ARTICLE
Light-controlled phosphorylation in the TrkA-Y785 site by photosensitive UAAs activates the MAPK/ERK signaling pathway
SHU ZHAO1,*, SHIXIN YE2
1 School of Life Sciences, Nantong University, Nantong, 226019, China
2 Institute National de la Sante et de la Recherche Med
icale (INSERM) U1195, Bicetre Hospital, Paris-Saclay University, Le Kremlin-Bicêtre, 94276, France
* Corresponding Author: Shu Zhao,
BIOCELL 2023, 47(6), 1377-1388. https://doi.org/10.32604/biocell.2023.023874
Received 19 May 2022; Accepted 06 September 2022; Issue published 19 May 2023
Abstract
Background: This paper aims to establish a light-controlled phosphorylation detection method at the Y785 site
of tropomyosin receptor kinase A (TrkA) receptor in mammalian cells by using genetic code expansion technology and
detecting the effects of optical activation of this site on the downstream MAPK/ERK pathway. The study is based on the
current situation that the regulatory mechanism of TrkA phosphorylation has not been fully elucidated.
Methods: Two
photosensitive unnatural amino acids, p-azido-L-phenylalanine (AzF) and photo-caged tyrosine (ONB) were introduced
into the TrkA-Y785 site by genetic code expansion technology and site-directed mutagenesis. Western blotting and laser
confocal imaging were conducted to analyze the effects of this site on activating the MAPK/ERK pathway and nerve cell
differentiation before and after photostimulation.
Results: Our results supplemented the light-controlled results of the
TrkA-Y785 site based on our previous research and verified that Y785 also makes important contributions in
regulating the MAPK/ERK pathway.
Conclusion: This study demonstrated the significant contributions of the TrkAY785 site in regulating the ERK pathway by precisely controlling the phosphorylation state of a single tyrosine site.
Keywords
Cite This Article
APA Style
ZHAO, S., YE, S. (2023). Light-controlled phosphorylation in the trka-y785 site by photosensitive uaas activates the MAPK/ERK signaling pathway. BIOCELL, 47(6), 1377-1388. https://doi.org/10.32604/biocell.2023.023874
Vancouver Style
ZHAO S, YE S. Light-controlled phosphorylation in the trka-y785 site by photosensitive uaas activates the MAPK/ERK signaling pathway. BIOCELL . 2023;47(6):1377-1388 https://doi.org/10.32604/biocell.2023.023874
IEEE Style
S. ZHAO and S. YE, "Light-controlled phosphorylation in the TrkA-Y785 site by photosensitive UAAs activates the MAPK/ERK signaling pathway," BIOCELL , vol. 47, no. 6, pp. 1377-1388. 2023. https://doi.org/10.32604/biocell.2023.023874