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Bioinformatic analysis of lncRNA-associated competing endogenous RNA regulatory networks in synovial tissue of temporomandibular joint osteoarthritis

CHUYAO WANG1,2,#, CHUAN LU2,#, LUXIANG ZOU2,*, DONGMEI HE2,*

1 School of Stomatology, Qingdao University, Qingdao, 266000, China
2 Department of Oral Surgery, Ninth People’s Hospital, Shanghai Jiao Tong University School of Medicine, Shanghai Key Laboratory of Stomatology & Shanghai Research Institute of Stomatology, National Clinical Research Center of Stomatology, Shanghai, 200011, China

* Corresponding Author: Dongmei He, email
# Co-first authors

(This article belongs to the Special Issue: Bioinformatics Study of Diseases)

BIOCELL 2023, 47(6), 1293-1306. https://doi.org/10.32604/biocell.2023.028199

Abstract

Background: Temporomandibular joint osteoarthritis (TMJOA) is an end-stage disease that seriously affects the patients’ quality of life. Molecular insights in advancing our understanding of TMJOA are the need of the hour. Methods: We performed RNA high-throughput sequencing and bioinformatics analysis of differentially expressed (DE) long non-coding RNA (lncRNAs), microRNAs (miRNAs), and messenger RNA (mRNAs) in human synovial TMJOA tissues. Firstly, synovium samples of TMJOA patients and non-TMJOA controls were collected for highthroughput sequencing of lncRNAs, miRNAs, and mRNAs. We then performed biological function analysis of the top 100 mRNAs with more than 2-fold differential expression, and their upstream regulated miRNAs and lncRNAs were predicted separately. Intersections between predicted miRNAs/lncRNAs and differentially expressed miRNAs/ lncRNAs were determined, respectively. Regulatory networks among the selected lncRNAs, miRNAs, and mRNAs were constructed. Finally, Western Blotting and reverse transcription-quantitative polymerase chain reaction (RTqPCR) were used to explore the function of selected lncRNA and mRNAs. Results: Our analysis showed that the only upregulated lncRNAxist, was involved in intracellular protein degradation, cartilage matrix degradation, and osteoclast differentiation through four regulatory axes: miR-1271-5p/ctsb, miR-365a-3p/mmp3, miR-199a-3p/fos, and miR-27b-3p/ miR-1271-5p/grb2. RT-qPCR results showed that xist expression increased in both TMJOA synovial tissue and inflammatory-stimulated synovial cells. Inhibition of xist could promote the proliferation of synovial cells upon inflammatory stimulation, and xist could positively regulate the expression of GRB2. Targeting GRB2 could inhibit the expression of matrix degrading enzymes in synovial cells, thereby attenuating the inflammatory environment. Conclusion: Given the current lack of reports of lncRNA regulation in TMJOA synovitis, our results revealed that xist was significantly differentially expressed in human synovial TMJOA and could regulate the expression of GRB2, which may play a role in the progression of TMJOA.

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APA Style
WANG, C., LU, C., ZOU, L., HE, D. (2023). Bioinformatic analysis of lncrna-associated competing endogenous RNA regulatory networks in synovial tissue of temporomandibular joint osteoarthritis. BIOCELL, 47(6), 1293-1306. https://doi.org/10.32604/biocell.2023.028199
Vancouver Style
WANG C, LU C, ZOU L, HE D. Bioinformatic analysis of lncrna-associated competing endogenous RNA regulatory networks in synovial tissue of temporomandibular joint osteoarthritis. BIOCELL . 2023;47(6):1293-1306 https://doi.org/10.32604/biocell.2023.028199
IEEE Style
C. WANG, C. LU, L. ZOU, and D. HE "Bioinformatic analysis of lncRNA-associated competing endogenous RNA regulatory networks in synovial tissue of temporomandibular joint osteoarthritis," BIOCELL , vol. 47, no. 6, pp. 1293-1306. 2023. https://doi.org/10.32604/biocell.2023.028199



cc This work is licensed under a Creative Commons Attribution 4.0 International License , which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
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